LOCUS       NM_175306               4585 bp    mRNA    linear   ROD 04-AUG-2023
DEFINITION  Mus musculus phosphatase and actin regulator 4 (Phactr4),
            transcript variant 1, mRNA.
ACCESSION   NM_175306 XM_911911
VERSION     NM_175306.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Wang Y, Yang Z, Gu J, Zhang Y, Wang X, Teng Z, Wang D, Gao L, Li W,
            Yeh S and Han Z.
  TITLE     Estrogen receptor beta increases clear cell renal cell carcinoma
            stem cell phenotype via altering the circPHACTR4/miR-34b-5p/c-Myc
            signaling
  JOURNAL   FASEB J 36 (2), e22163 (2022)
   PUBMED   35061326
  REMARK    GeneRIF: Estrogen receptor beta increases clear cell renal cell
            carcinoma stem cell phenotype via altering the
            circPHACTR4/miR-34b-5p/c-Myc signaling.
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Hooper JE, Jones KL, Smith FJ, Williams T and Li H.
  TITLE     An Alternative Splicing Program for Mouse Craniofacial Development
  JOURNAL   Front Physiol 11, 1099 (2020)
   PUBMED   33013468
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4585)
  AUTHORS   Rohacek AM, Bebee TW, Tilton RK, Radens CM, McDermott-Roe C, Peart
            N, Kaur M, Zaykaner M, Cieply B, Musunuru K, Barash Y, Germiller
            JA, Krantz ID, Carstens RP and Epstein DJ.
  TITLE     ESRP1 Mutations Cause Hearing Loss due to Defects in Alternative
            Splicing that Disrupt Cochlear Development
  JOURNAL   Dev Cell 43 (3), 318-331 (2017)
   PUBMED   29107558
REFERENCE   4  (bases 1 to 4585)
  AUTHORS   Cho HM, Kim JY, Kim H and Sun W.
  TITLE     Phosphatase and actin regulator 4 is associated with intermediate
            filaments in adult neural stem cells and their progenitor
            astrocytes
  JOURNAL   Histochem Cell Biol 142 (4), 411-419 (2014)
   PUBMED   24748504
  REMARK    GeneRIF: The Phactr4 forms functional associations with
            intermediate filament networks in adult neural stem cells.
REFERENCE   5  (bases 1 to 4585)
  AUTHORS   Huet G, Rajakyla EK, Viita T, Skarp KP, Crivaro M, Dopie J and
            Vartiainen MK.
  TITLE     Actin-regulated feedback loop based on Phactr4, PP1 and cofilin
            maintains the actin monomer pool
  JOURNAL   J Cell Sci 126 (Pt 2), 497-507 (2013)
   PUBMED   23203801
  REMARK    GeneRIF: Data discloses the central role of Phactr4 in a feedback
            loop, where actin monomers regulate their own number via the
            activation of a key regulator of actin dynamics.
REFERENCE   6  (bases 1 to 4585)
  AUTHORS   Amante FH, Stanley AC, Randall LM, Zhou Y, Haque A, McSweeney K,
            Waters AP, Janse CJ, Good MF, Hill GR and Engwerda CR.
  TITLE     A role for natural regulatory T cells in the pathogenesis of
            experimental cerebral malaria
  JOURNAL   Am J Pathol 171 (2), 548-559 (2007)
   PUBMED   17600128
REFERENCE   7  (bases 1 to 4585)
  AUTHORS   Kim TH, Goodman J, Anderson KV and Niswander L.
  TITLE     Phactr4 regulates neural tube and optic fissure closure by
            controlling PP1-, Rb-, and E2F1-regulated cell-cycle progression
  JOURNAL   Dev Cell 13 (1), 87-102 (2007)
   PUBMED   17609112
  REMARK    GeneRIF: Phactr4 is critical for the spatially and temporally
            regulated transition in proliferation through differential
            regulation of PP1 and the cell cycle during neurulation and eye
            development
REFERENCE   8  (bases 1 to 4585)
  AUTHORS   Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M,
            Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A and
            Cortese R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 4585)
  AUTHORS   Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng
            EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC,
            Babbitt PC and Ferrin TE.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 4585)
  AUTHORS   Kasarskis A, Manova K and Anderson KV.
  TITLE     A phenotype-based screen for embryonic lethal mutations in the
            mouse
  JOURNAL   Proc Natl Acad Sci U S A 95 (13), 7485-7490 (1998)
   PUBMED   9636176
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY294063.1, BB849287.1, BC075672.1 and AK029356.1.
            
            [WARNING] On Jan 11, 2024 this sequence was replaced by
            NM_175306.5.
            
            On Jun 17, 2009 this sequence version replaced NM_175306.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC075672.1, AK049209.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                BY294063.1         44-60
            18-38               BB849287.1         13-33
            39-2640             BC075672.1         1-2602
            2641-2939           BC075672.1         2605-2903
            2940-4563           AK029356.1         220-1843
            4564-4585           BC075672.1         4528-4549
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 65.24 cM"
     gene            1..4585
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /note="phosphatase and actin regulator 4"
                     /db_xref="GeneID:100169"
                     /db_xref="MGI:MGI:2140327"
     exon            1..102
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            103..156
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..2225
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /note="isoform 1 is encoded by transcript variant 1;
                     humdy; protein Humpty dumpty"
                     /codon_start=1
                     /product="phosphatase and actin regulator 4 isoform 1"
                     /protein_id="NP_780515.2"
                     /db_xref="CCDS:CCDS18724.1"
                     /db_xref="GeneID:100169"
                     /db_xref="MGI:MGI:2140327"
                     /translation="MEDPSEEAEQPSGDPGMGMDSVEAGDTTPPTKRKSKFSALGKIF
                     KPWKWRKKKSSDKFKETSEVLERKISMRKPREELVKRGVLLEDPEQDGEDSGKLSHAA
                     LKNGHTTPIGSARSSSPVLVEEEPERSLRNLTPEEESKKRLGSTGSQPNSEAEPGPEH
                     APKQPLLPPKRPLSSSCEAKEVPAGSTARSVSSTSGSTTVTSAATTAATDMTKTVKSF
                     VGPTPAPAPAPRTLPAAPASANTAATTTAPAKQPPIPPPKPAQRNSNPIIAELSQAMN
                     SGTVLSKPSPPLPPKRGIPSTSIPSLEPAASFTTKTANDQREKTVSLCLEPPLIIPPS
                     SPSPPLPTHIPPEPPRSPLVPAKTFQIVPEVEFSSSSDLFQDISQQEDQKTEVPKKIQ
                     DQSFGESHIPSRLPPLPLHIRIQQALTSPLPVTPPLEGTHRAHSLLFENSDSFSEDTG
                     TLGRTRSLPITIEMLKVPDDEEEEQTCPFVEDVTSTSATPSLPLCLREEEKESDSDSE
                     GPIKYRDEEEDDDDDESHQSALANRVKRKDTLAMKLSSRPSEPETNLNSWPRKSKEEW
                     NEIRHQIGNTLIRRLSQRPTAEELEQRNILQPKNEADRQAEKREIKRRLTRKLSQRPT
                     VAELLARKILRFNEYVEVTDAHDYDRRADKPWTKLTPADKAAIRKELNEFKSSEMEVH
                     VDSKHFTRYHRP"
     exon            157..330
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            331..411
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            412..570
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            571..945
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1540
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1722
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1876
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..1932
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            1933..1994
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2132
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2209
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
     exon            2210..4571
                     /gene="Phactr4"
                     /gene_synonym="3110001B12Rik; C330013F19Rik; mKIAA4120"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcccctgggg ccgaaggctc tggctggagc caggcggcca gagaccaggt aggatttccg
       61 ggagaaagtg ctggggaacc cgaggaggca gcggcgactt ggaaactact ccttctctcc
      121 ctacactggt tagcagtggc atggaagacc catcggaaga agcagagcag ccctctggag
      181 acccaggcat gggcatggac agtgtggaag caggggacac aacacctccc accaaaagga
      241 aaagcaagtt ctcagcgtta ggcaagatct tcaagccgtg gaaatggagg aaaaaaaaaa
      301 gcagtgataa gttcaaagag acatcagaag ttttagagcg aaaaatatct atgcgaaaac
      361 caagagaaga actggttaaa cgaggggttc tgttggagga ccccgagcag gatggtgagg
      421 attcaggaaa gctgagccat gctgcattaa agaacggaca caccaccccc ataggcagtg
      481 ccaggtcctc tagcccagtc ctggtagaag aagagccaga gagaagtctc aggaacctca
      541 ctccagaaga ggagtcaaag aagagacttg gctcaactgg aagccagcct aactctgaag
      601 ccgagcctgg tcctgagcat gcacctaagc agcctctgct gccccccaag agacccttgt
      661 cctcttcttg tgaagcaaag gaggttccgg ctggcagtac agcaaggtcc gtctcctcca
      721 cctctggctc taccaccgtg acatctgctg ccaccactgc tgccacagac atgacaaaga
      781 ctgttaagtc ctttgtgggc cccacccccg ctcctgcccc cgcccccagg actctgcctg
      841 ctgctcctgc cagtgctaac actgctgcca ccactacagc ccctgccaag caacccccca
      901 tcccaccccc taaaccagct cagagaaaca gcaaccccat cattgctgaa ctatcccaag
      961 caatgaacag tggcacagtg ctgtcgaaac catccccacc cttgccaccc aagagaggca
     1021 ttccatcaac ttcgataccc agcttggagc ctgctgcttc gttcacaact aagacagcaa
     1081 atgatcaaag agaaaaaact gtctctttgt gtttggaacc accactgata atcccgcctt
     1141 cgtccccatc acccccactg cctacccaca tacctcccga gcctccacgg tcccctctag
     1201 tgcctgctaa gacttttcaa attgtgccag aggttgagtt ttcctcttct tcagatctat
     1261 tccaggacat ttcccagcag gaggatcaga aaacagaagt ccccaagaag atacaggatc
     1321 agagctttgg ggagtcccat ataccctcca ggctgccccc actcccactg catatccgaa
     1381 ttcagcaggc ccttaccagc ccccttcctg tgacccctcc cttggaaggc actcacagag
     1441 cccattcact gctttttgag aacagtgaca gcttctccga ggacactggt accctgggtc
     1501 gtaccaggtc acttcccatc acgattgaga tgttgaaagt tccagatgat gaagaggaag
     1561 agcagacctg cccgtttgtt gaagacgtga cgtctacctc agccactcct agcctaccac
     1621 tgtgtctgcg agaggaagag aaggagagtg actcagattc agagggtccc attaagtacc
     1681 gagatgaaga agaagatgat gatgatgatg aaagccatca gagtgccctg gccaacagag
     1741 tgaagaggaa ggacacgctg gcaatgaagc tgagcagcag acccagtgaa ccagagacca
     1801 acctgaattc ttggcctcgt aaaagcaagg aggagtggaa tgaaatacgg caccagatcg
     1861 ggaacacgct gatccggcgg ctgagtcaga gaccaacagc agaagaattg gagcagcgga
     1921 acatactaca gcctaaaaat gaagctgatc ggcaagcaga gaaacgggaa atcaagcgtc
     1981 gcctcactag aaagctcagt caaaggccaa ctgtggctga actgcttgcc aggaagattc
     2041 tgcggtttaa tgagtatgtg gaagtaactg atgctcatga ctatgaccgg cgagcagaca
     2101 aaccatggac caaactgact cctgctgaca aggctgccat aaggaaagaa ttaaatgaat
     2161 ttaagagctc ggagatggag gttcatgtag acagcaaaca ctttacacgc taccatcgcc
     2221 catgaagctg aagcctgaga aaggagctaa aggaccacag gaaaaggaaa agcatggctg
     2281 gtctgcttct tgtctccagg gcgagcacag cacttctcac acagccagag ctgctctggg
     2341 atctgggcag ggcaggcagc gcttgtacgc gtgtccttgt gctcccctta gagaagaatg
     2401 agacaccgat gctttgagcc ttttaacaac actgcagtag acttgagggt gtctctcacc
     2461 agccacagaa gcacagggcc tcttacaggt tttgagtttt tctggctacg gcacacgctg
     2521 ccctaagaca acttggtgtg ctgaggccgt ttgtaccgtg agccttacct gtctgtcctg
     2581 tctgagagcc gctcagggag gctcattcca accctcaggt tctcttacca ggaaaaaaaa
     2641 cattcggaca caccagccct tccagcaaag gcccgcttcc agaaaagtgc ttggacttct
     2701 ctgcataagg tctgaccaga aggaagccag tgcaggctcc ggcctgttcc tccctccatt
     2761 tggcacttgt catcctaggt cctttttgtt ttgagacaag atcttatcct gtggctcagg
     2821 ctggcctcaa actcactgtg tagcccaggc tggcctcaaa cttctgtctc agcctcccaa
     2881 gtgctgagat tataagcatg agccttcatg ctctcatcct gatttcaaag tgagcttaaa
     2941 gaaatgggga aaatccattc attatgtagt ctgagctgta atcagaatcc aaatgatcaa
     3001 atgagaaagc cacgtgcatt ttctatgtac aaaccaagaa aaaggcctta ctgttaatgc
     3061 cggtttggca gagaactgac tcacggggca gactttatta tacgttccct gtaccaaatg
     3121 atactataaa tcactttggc ctgtgggagc actgtgtggg agcactgtgc gctacaggtg
     3181 cccatgctca ctccttggtg ctgttggccc tccttactgc acagcccctc tactgaatta
     3241 gcccctcccc ctcccgacaa cgaacacctc acttgccttc ccattcctgt tgcagccagg
     3301 cctccccact tgtattgggg tgcagatttg gttatgtttt tcaaagaact tttttcctga
     3361 aacccacaca gccttactgt tcctgcctgg attctactta cattgtaagt ggaagggaaa
     3421 tgtaactggg tttgtcgctt ttctttttgt tttagtctat acctcagtat gtagcccagg
     3481 ctagcctttg acttgaagtc ttccttactc agccttccca gtctgcgatc atcgagggca
     3541 acggccacac ccagcttcta ctcaccttaa cagctagtgg attgtggctc ttaaaaaggc
     3601 tttgctttct tgtttgtttg ttttttgctt tattctattt cctgtctgta ttctttcagc
     3661 aattttctcc ttgtcagcat cacacttatt aaagagttag aatatagggc aaacaaaaaa
     3721 agctaaagtg accttaaaac tagtactttc ttctctatga aggaaaaagt gcttttgttt
     3781 gttttttctc ttcctgccat gtagtttctg tctatggaac aaagactcct tcccactccg
     3841 tccccatctc ctcaaacctt gatattgctt gagtaaagga agagaaaaca acagttctgt
     3901 caagtctgtg agttgtctag atacaaggtt gccagagctg ctaacctttg cgattacatc
     3961 ctgaccaagg ctcagttctc acaagcaagg ccggtgctcc gcccctgctc cgcccctgct
     4021 ccgccctgct ccaccctgct ccgccccgtt tctccttctc tgtcccaggt ttagagagta
     4081 ctttgcatat gcctgtgctg tctggggtgt ggtaaccttt tttttagaca gggtcttatg
     4141 tagcctcact atgtagccaa ggatcccctg cctccacctc ccgagtacag ggctcacaga
     4201 tgcacccacc atacctgccg gccagcaacg gcttctctca gctgttctcc ctcacgtgcc
     4261 aatcctgagc tcccgtctgc ttgtgtgtgc catcaagggc tctgactctg ttcatctcat
     4321 ctgtcttctg aagcaggata tgttagggct gcatctaggc agtggttttg tttttatgta
     4381 tataaaagtt gattatgtgt cttctttctg taagtggaag cttacagtcc taatgtaaag
     4441 gacttaaagc ctcgctgtcg catgttaaga tgcttcccta ctgtacagaa tcctgtaaaa
     4501 tacataatta cgttgtatat cagccttcct accaatctta attattaaag tattttagaa
     4561 tatcaaaaaa aaaaaaaaaa aaaaa
//
