LOCUS       NM_028806               5100 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phosphatase and actin regulator 3 (Phactr3),
            transcript variant 1, mRNA.
ACCESSION   NM_028806 XM_130742
VERSION     NM_028806.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5100)
  AUTHORS   Miyata,T., Kikuchi,K., Ihara,D., Kaito,M., Ishibashi,Y.,
            Hakamata,T., Yamada,T., Ishikawa,M., Mizukoshi,M., Shoji,S.,
            Fukuchi,M., Tsuda,M., Hida,Y., Ohtsuka,T., Kaneda,M. and Tabuchi,A.
  TITLE     Neuron-enriched phosphatase and actin regulator 3 (Phactr3)/
            nuclear scaffold-associated PP1-inhibiting protein (Scapinin)
            regulates dendritic morphology via its protein phosphatase
            1-binding domain
  JOURNAL   Biochem Biophys Res Commun 528 (2), 322-329 (2020)
   PUBMED   32423795
  REMARK    GeneRIF: Neuron-enriched phosphatase and actin regulator 3
            (Phactr3)/ nuclear scaffold-associated PP1-inhibiting protein
            (Scapinin) regulates dendritic morphology via its protein
            phosphatase 1-binding domain.
REFERENCE   4  (bases 1 to 5100)
  AUTHORS   Park,C.S., Rehrauer,H. and Mansuy,I.M.
  TITLE     Genome-wide analysis of H4K5 acetylation associated with fear
            memory in mice
  JOURNAL   BMC Genomics 14, 539 (2013)
   PUBMED   23927422
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5100)
  AUTHORS   Kim,J.Y., Choi,S.Y., Moon,Y., Kim,H.J., Chin,J.H., Kim,H. and
            Sun,W.
  TITLE     Different expression patterns of Phactr family members in normal
            and injured mouse brain
  JOURNAL   Neuroscience 221, 37-46 (2012)
   PUBMED   22766235
  REMARK    GeneRIF: This study demonistrated that Phactr1 appeared to be
            expressed in post-mitotic neurons in developmental brain.
REFERENCE   6  (bases 1 to 5100)
  AUTHORS   Worch,S., Hansmann,I. and Schlote,D.
  TITLE     Paramutation-like effects at the mouse scapinin (Phactr3) locus
  JOURNAL   J Mol Biol 377 (3), 605-608 (2008)
   PUBMED   18295233
  REMARK    GeneRIF: Phactr3 exon 1C splice variant is potentially strain
            specific regulated, leading to the expression of only one allele of
            the reciprocal crosses.
REFERENCE   7  (bases 1 to 5100)
  AUTHORS   Worch,S., Fiedler,E., Hansmann,I. and Schlote,D.
  TITLE     Genomic organization and expression pattern of scapinin (PHACTR3)
            in mouse and human
  JOURNAL   Cytogenet Genome Res 115 (1), 23-29 (2006)
   PUBMED   16974080
  REMARK    GeneRIF: Expression analysis in the mouse revealed a
            tissue-specific complex transcription pattern in the brain and a
            specific pattern was observed during prenatal development
REFERENCE   8  (bases 1 to 5100)
  AUTHORS   Allen,P.B., Greenfield,A.T., Svenningsson,P., Haspeslagh,D.C. and
            Greengard,P.
  TITLE     Phactrs 1-4: A family of protein phosphatase 1 and actin regulatory
            proteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (18), 7187-7192 (2004)
   PUBMED   15107502
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK135144.1, BC058621.1 and AL772217.10.
            
            [WARNING] On Oct 26, 2024 this sequence was replaced by
            NM_028806.3.
            
            On Apr 28, 2010 this sequence version replaced NM_028806.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK135144.1, BC058621.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-84                AK135144.1         2-85
            85-2676             BC058621.1         1-2592
            2677-5100           AL772217.10        94603-97026
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 99.67 cM"
     gene            1..5100
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /note="phosphatase and actin regulator 3"
                     /db_xref="GeneID:74189"
                     /db_xref="MGI:MGI:1921439"
     exon            1..567
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     CDS             453..2129
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /note="isoform 1 is encoded by transcript variant 1;
                     scaffold-associated PP1-inhibiting protein"
                     /codon_start=1
                     /product="phosphatase and actin regulator 3 isoform 1"
                     /protein_id="NP_083082.1"
                     /db_xref="CCDS:CCDS17158.1"
                     /db_xref="GeneID:74189"
                     /db_xref="MGI:MGI:1921439"
                     /translation="MAASEDGSSCLVSRGRSQSDPSFLSDSSATSTDAGENPDEMDQT
                     PPARSEPLVSGIRTPPVRRNSKLATLGRIFKPWKWRKKKNEKLKQTTSALEKKMAGRQ
                     GREELIKQGLLEMMEQDSENKACSPKEGSQPVQSEPPAGEQETLTSEGAQPGSPSASG
                     TDQVSQDELLSSDAHLDDTANIPSASTAEEADAGSLLPTTDEPSQALAGSDSLDSPPR
                     SLERSVSQLPSPPLLPTPPPKASSKATKNVTGQAALFQGPSMKNNEPALRGQLATPTG
                     SPHVTTVHRPLPPSRVMEELHRALATKHRQDSFQGRECRGSPKKRMDVRLSRTSSMER
                     GKERDEAWSFDGASENKWTATKDSEENKENLMLSSELKDDMLLYQDEEALNDSIISGT
                     LPRKCKKELLAVKLRNRPSKQELEDRNIFPRRTDEERQEIRQQIEMKLSKRLSQRPAV
                     EELERRNILKQRNDQTEQEERREIKQRLTRKLNQRPTVDELRDRKILIRFSDYVEVAR
                     AQDYDRRADKPWTRLSAADKAAIRKELNEYKSNEMEVHASSKHLTRFHRP"
     exon            568..729
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            730..807
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            808..990
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1200
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1375
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..1623
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1777
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1833
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1895
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..2036
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2113
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..5100
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2651..2656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /note="hexamer: ATTAAA"
     polyA_site      2680
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /note="major polyA site"
     regulatory      2808..2813
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /note="hexamer: AATATA"
     polyA_site      2838
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
     regulatory      3047..3052
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /note="hexamer: AATACA"
     polyA_site      3063
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
     regulatory      3077..3082
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /note="hexamer: AATAAA"
     polyA_site      3107
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
     regulatory      3587..3592
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
                     /note="hexamer: ATTAAA"
     polyA_site      3608
                     /gene="Phactr3"
                     /gene_synonym="1500003N10Rik; 4930415A02Rik; H17739;
                     mKIAA4224; Scapin1; Scapinin"
ORIGIN      
        1 agattaaagc aattgcaaga gcaaagattc tcctttttcc ctttttttcg ggggggtggg
       61 gggtggggtg gggggaggga gcacccccag acattcctgg acatctcccc cagccccaag
      121 cacgccataa acactgacaa gaaaaagttt ttatttcctg gttcaacttt tttttttccc
      181 ctggaatata agcagaagaa tgggaataaa tacgaataaa taacaaagcg aggccgcgca
      241 agccgggatg cgcctggctg cagcctgcaa ggcttttgtc tccccaccct gaagccagcc
      301 tggcgtcctc ctccgttctt tttctttcct ggtccctttt gcttttcctt ttaaaaaaga
      361 cgccctctcc agccccctcg ccggtgacct tggccgccta ggatgctctg attccgcatc
      421 tccctccaac ttgcccctgc gtcgcctttc ccatggccgc atccgaggac ggcagcagct
      481 gccttgtgtc gcggggccgc tcgcagagtg accccagctt cctcagcgac tcctcagcca
      541 cctccacgga cgccggggag aacccagatg agatggacca aactccccca gcacgctcag
      601 agcctctggt ctcagggatt cgaactcccc ctgtgaggag gaacagcaag ctggccaccc
      661 tgggcaggat cttcaaacct tggaaatgga ggaaaaagaa aaacgagaaa ctgaagcaga
      721 cgacgtcagc gctggagaag aagatggctg gcaggcaagg ccgggaggaa ctcatcaagc
      781 agggactgct ggagatgatg gagcaagatt ctgaaaataa ggcatgcagt cccaaggaag
      841 gctcccaacc tgtgcagagt gagccgcccg ctggcgagca ggagacgctg acctcggagg
      901 gcgcccagcc cggaagcccc tcggccagtg gaacagatca ggtgtcccag gatgagcttc
      961 tgtcctccga tgctcatctg gatgatacag ccaatatacc gtctgcatcc actgcggaag
     1021 aagcagacgc tggcagcctc ctgcccacca ctgatgagcc ctctcaagcc ttagctgggt
     1081 ctgactccct ggacagtcct cccagatccc tggagagatc tgtgagccag ctccccagcc
     1141 ctccactgct gcccaccccg ccacccaagg ccagctctaa agccacaaaa aatgtcacag
     1201 gacaagctgc gctcttccaa ggccccagca tgaagaacaa tgagccggct ctcagaggac
     1261 agcttgccac ccccacaggg tcccctcatg tcaccactgt ccaccggcca ctgcccccca
     1321 gccgcgtgat ggaggagctg catagggcac tggctacaaa gcaccgtcag gacagttttc
     1381 aagggcggga atgcagaggg tctccaaaga agcggatgga tgtgcgtctg tctaggacgt
     1441 ccagcatgga gcggggcaag gagagggacg aggcgtggag ctttgacggg gcctcagaga
     1501 acaagtggac ggctaccaaa gactccgagg agaacaagga gaatctgatg ctgagctccg
     1561 agctcaagga tgacatgctc ctgtatcagg acgaggaggc gctcaatgac tccatcatct
     1621 cgggaacttt gccgaggaaa tgcaagaagg agttgctggc agtgaagctg aggaaccggc
     1681 cgagcaagca ggagctagag gaccggaaca tcttccccag gaggaccgat gaggagagac
     1741 aggagatccg acagcagata gagatgaagc tgtccaaaag gctgagccag agacccgccg
     1801 tggaggaact ggagagaaga aatatcctga aacaaaggaa tgatcagaca gagcaggaag
     1861 aaaggagaga aatcaagcaa agattaacaa gaaagcttaa ccagagaccc actgttgatg
     1921 aattgagaga cagaaaaatt ctgatccgtt tcagtgatta tgtggaagta gcaagagcac
     1981 aggactatga caggagggca gacaagccct ggacaagact gtcagcagca gataaggcag
     2041 caattcgtaa agaattaaat gaatataaaa gtaacgaaat ggaggtacac gcatccagca
     2101 agcacttgac aagattccac aggccataga gattttcttc tgagaagaat ttgtgtttaa
     2161 tttttgatac caacactgaa cattcatcag ggaactttcc tcaagtctaa ctgctgtgtt
     2221 tgtgagaaga gcagaatcac ggagacgcaa tcctgaccac tgactacaag aggagcaacc
     2281 cagaggatac actcccctct ctggtgtctg aggagtgtga accgttgggg tcactttagg
     2341 cccagagtaa ctctcccaga agaaaacttg tttgccttca accttgtttt ccagttctgc
     2401 agcgtcatgg aggatgggcg ggcgccatgc atgtgcaggc tcacactccc agcctccgcc
     2461 acgccacgag ggacctgtga cccagtgtca ttcagtattt tgttgagaag acatttttat
     2521 cctaatgatt tgaaaactaa gttcatgtta tagagtggtt tactgtacta tactttctct
     2581 tttttatatg atgtctaaca aaaaaaaaat acagctccaa cattttgatt cctgttaatt
     2641 ttgttcttta attaaatgac tacttattac aggaaatgaa taaaggcttt cactttcttg
     2701 gggttgggat gaggtatagc tggggacttt gaaaaaaaaa aagtcagcgc ttttgaatat
     2761 aaacacaaga aaggaaatgc caactttgtt ctctgtagat tctgtcaaat atatacaggc
     2821 ttgtgccttg acaaactagt taccattttt tcaagttaat taattgaaaa tttatgaaag
     2881 agtattgaac acatttaacc tagaaggaac ttactggaaa ccctaacaaa aggaaaatct
     2941 ataccttctt taagaaaagt tgtaagtttt catttgtctg cattatcaaa acccctatta
     3001 taagaggttt agatggaatg aaggtttgga gtaagtagtt tactgaaata caaggggaag
     3061 aaatgagctg acaaacaata aaaatcccct acgtttgttt atgggttttt ttgttgtttt
     3121 tatttatttg atttgttttt taagttttct ctcagaaaga agccttccta cccctctgcc
     3181 caggataatg gccagcggta aacaatggct tggagctcaa agacctacac tcaaatgtag
     3241 gcctctgtgc acttcaagca ggtgacttgc tctttaggat ggcctcacac ttctgtaata
     3301 tgtggtaact aacatgctgg cacaagccag gcacactctc ggcactcagt ttgttagcta
     3361 gagagcactg agaccaagca agtgtactga acaaattgac tcagctacca catcccattg
     3421 ctctcccagc tctctaagac ttcttaggac atttgtggca gttctctggc taaagaggct
     3481 gatgatcagt tctgatacta gcaaacattc cactaattga attcattttc aaaaaagaaa
     3541 ttacagcaga acgattctag ctttacctgt tctatgatgt gtgtgaatta aactttttgt
     3601 gggtggaagc cagtgactga atttcacaag catgtagaat atttgaatat ggtagtgtct
     3661 attgattcct tagttgtggc cagttaatat caaagcatgt ctttccctat ttcaagatac
     3721 atatcaacag ctcctatttt atattttgct tagtttaaaa catgagtggt ccaaagtttt
     3781 acacacacac acacacacac acacacacac acacacacac acacacacca aaatttcagt
     3841 ctctaagaaa tacccacata actattacag gaaaagggga acctgctgct cttctagaaa
     3901 tagcactaac ccacagggga ttaactctgc ttagagcgga gccaagtgga gactttctct
     3961 gctccagcca agtcccatag atagtggctg aaaaaaactt aaggtaaatt ataggatatc
     4021 caaggaagga tttcagtttg gttaaggtca gagctgcaat ataactaaaa gtgttagaaa
     4081 ttttcatttt aatcaaggaa ttggcaatag tacccaattt ctaccatttt agttttatta
     4141 atcataactt tctatgtttc atattcctat gagtgtcata aggggaatta ggagatttga
     4201 tagataaatc tgataaactc cttaactttg cagtgactaa gtgtgtaacc catcttaagc
     4261 agcttccaaa gtagattcac accatataga gcagtccact tatatggcca tggtgctatg
     4321 catatgcagt cacctatgac aatctgagct tgaaaacaaa atttattttc aagtctagag
     4381 gcttaagaaa agtatttctg ttgccttaga ggtctttatt cctagtggca ctgagaaggt
     4441 tatgtgtcaa ctttacaaat catttgctta cattttcaca aaatgtgcta gagcaaaatt
     4501 acttaatgca agtcttaaaa agattacaaa ccttacttaa acatctgtaa ctttaaggta
     4561 agtattaaaa atttaaagaa atctaaagtt tagttttctg acctgaagaa tttttggatc
     4621 cttgaatttg ggaaaacaaa ggcctcctag gatcaagagg tccatcttgt ggaaatctat
     4681 ggtaactaca ggtttaagct ttccatcaca tagtagcaag tcagattcta gctgtagtca
     4741 gaagcaaatt caagtccatt aaaatatggg caccagggtg gtgtacttat gtaaacccag
     4801 ctccttggat gaagatcagg gttttaagcc agccttagct acataagact cagcataaaa
     4861 ccaaaccaaa ccgagcaaaa ccccagattt gtcctgaatc ttttcagtac ccatttagca
     4921 agttattttt caatggagca actgaaaata tgcaaataaa accatatata ccagtgtctt
     4981 caaacagaca atacctgtaa gatatcccta actccatctt ggtgcactga agtacactgc
     5041 ttaagcaatg ggaagctagc agtcatggtt ctgtgagaat aacagtttta tattcagagc
//
