LOCUS       NM_001005740            5350 bp    mRNA    linear   ROD 06-OCT-2024
DEFINITION  Mus musculus phosphatase and actin regulator 1 (Phactr1),
            transcript variant 2, mRNA.
ACCESSION   NM_001005740
VERSION     NM_001005740.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Xu,Z., Sadleir,L., Goel,H., Jiao,X., Niu,Y., Zhou,Z., de
            Valles-Ibanez,G., Poke,G., Hildebrand,M., Lieffering,N., Qin,J. and
            Yang,Z.
  TITLE     Genotype and phenotype correlation of PHACTR1-related neurological
            disorders
  JOURNAL   J Med Genet 61 (6), 536-542 (2024)
   PUBMED   38272663
  REMARK    GeneRIF: Genotype and phenotype correlation of PHACTR1-related
            neurological disorders.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5350)
  AUTHORS   Jiang,D., Liu,H., Zhu,G., Li,X., Fan,L., Zhao,F., Xu,C., Wang,S.,
            Rose,Y., Rhen,J., Yu,Z., Yin,Y., Gu,Y., Xu,X., Fisher,E.A., Ge,J.,
            Xu,Y. and Pang,J.
  TITLE     Endothelial PHACTR1 Promotes Endothelial Activation and
            Atherosclerosis by Repressing PPARgamma Activity Under Disturbed
            Flow in Mice
  JOURNAL   Arterioscler Thromb Vasc Biol 43 (8), e303-e322 (2023)
   PUBMED   37199156
  REMARK    GeneRIF: Endothelial PHACTR1 Promotes Endothelial Activation and
            Atherosclerosis by Repressing PPARgamma Activity Under Disturbed
            Flow in Mice.
REFERENCE   4  (bases 1 to 5350)
  AUTHORS   Wood,A., Antonopoulos,A., Chuaiphichai,S., Kyriakou,T., Diaz,R., Al
            Hussaini,A., Marsh,A.M., Sian,M., Meisuria,M., McCann,G.,
            Rashbrook,V.S., Drydale,E., Draycott,S., Polkinghorne,M.D.,
            Akoumianakis,I., Antoniades,C., Watkins,H., Channon,K.M., Adlam,D.
            and Douglas,G.
  TITLE     PHACTR1 modulates vascular compliance but not endothelial function:
            a translational study
  JOURNAL   Cardiovasc Res 119 (2), 599-610 (2023)
   PUBMED   35653516
REFERENCE   5  (bases 1 to 5350)
  AUTHORS   Ma,X., Su,M., He,Q., Zhang,Z., Zhang,F., Liu,Z., Sun,L., Weng,J.
            and Xu,S.
  TITLE     PHACTR1, a coronary artery disease risk gene, mediates endothelial
            dysfunction
  JOURNAL   Front Immunol 13, 958677 (2022)
   PUBMED   36091033
  REMARK    GeneRIF: PHACTR1, a coronary artery disease risk gene, mediates
            endothelial dysfunction.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5350)
  AUTHORS   Kim,J.Y., Choi,S.Y., Moon,Y., Kim,H.J., Chin,J.H., Kim,H. and
            Sun,W.
  TITLE     Different expression patterns of Phactr family members in normal
            and injured mouse brain
  JOURNAL   Neuroscience 221, 37-46 (2012)
   PUBMED   22766235
  REMARK    GeneRIF: This study demonistrated that Phactr1 appeared to be
            expressed in post-mitotic neurons in developmental brain.
REFERENCE   7  (bases 1 to 5350)
  AUTHORS   Zuchero,J.B., Belin,B. and Mullins,R.D.
  TITLE     Actin binding to WH2 domains regulates nuclear import of the
            multifunctional actin regulator JMY
  JOURNAL   Mol Biol Cell 23 (5), 853-863 (2012)
   PUBMED   22262458
REFERENCE   8  (bases 1 to 5350)
  AUTHORS   Kim,T.H., Goodman,J., Anderson,K.V. and Niswander,L.
  TITLE     Phactr4 regulates neural tube and optic fissure closure by
            controlling PP1-, Rb-, and E2F1-regulated cell-cycle progression
  JOURNAL   Dev Cell 13 (1), 87-102 (2007)
   PUBMED   17609112
REFERENCE   9  (bases 1 to 5350)
  AUTHORS   Trinidad,J.C., Specht,C.G., Thalhammer,A., Schoepfer,R. and
            Burlingame,A.L.
  TITLE     Comprehensive identification of phosphorylation sites in
            postsynaptic density preparations
  JOURNAL   Mol Cell Proteomics 5 (5), 914-922 (2006)
   PUBMED   16452087
REFERENCE   10 (bases 1 to 5350)
  AUTHORS   de Chaldee,M., Brochier,C., Van de Vel,A., Caudy,N.,
            Luthi-Carter,R., Gaillard,M.C. and Elalouf,J.M.
  TITLE     Capucin: a novel striatal marker down-regulated in rodent models of
            Huntington disease
  JOURNAL   Genomics 87 (2), 200-207 (2006)
   PUBMED   16359841
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC061691.1 and CD551063.1.
            
            Transcript Variant: This variant (2) represents the longest
            transcript and encodes the longest isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061691.1, AK048208.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2156              BC061691.1         1-2156
            2157-2392           CD551063.1         349-584
            2393-5350           BC061691.1         2389-5346
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 21.05 cM"
     gene            1..5350
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /note="phosphatase and actin regulator 1"
                     /db_xref="GeneID:218194"
                     /db_xref="MGI:MGI:2659021"
     exon            1..270
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1970
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /note="isoform 2 is encoded by transcript variant 2; RPEL
                     repeat containing 1"
                     /codon_start=1
                     /product="phosphatase and actin regulator 1 isoform 2"
                     /protein_id="NP_001005740.1"
                     /db_xref="CCDS:CCDS49251.1"
                     /db_xref="GeneID:218194"
                     /db_xref="MGI:MGI:2659021"
                     /translation="MCVSLLLSPPPPFRLSPSPSLHLLLLSARRATLLLPPTLMAASS
                     EDDIDRRPIRRVRSKSDTPYLAEARISFNLGAAEEVERLAAMRSDSLVPGTHTPPIRR
                     RSKFANLGRIFKPWKWRKKKSEKFKHTSAALERKISMRQSREELIKRGVLKEIYDKDG
                     ELSISNEDDSLENGQSLSSSQLSLPALSEMEPVPMPRDPCSYEVLQASDIMDGPVSEE
                     SPSASESGVLLSQDPSAKPVLFLPPKKSAAFPGDHEETPVKQLSLHKQPPALPPKPTA
                     RIANHLTDPGAPVKLPCLPVKLSPPLPPKKVLICMPVGGPELTLASYAAQKSSQQAVA
                     QHHHTVLPSQMQHQLQYGSHGQHLPSSTGTLPMHPSGCRMIDELNKTLAMTMQRLESS
                     EQRVPCSTSYHSSGLHSSDGITKAGPMGLPEIRQVPTVVIECDDNKENVPHEPDYEDS
                     PCLYGREEEEEEEDEDDDASLYTSSLAMKVCRKDSLAIKLSNRPSKRELEEKNILPRQ
                     TDEERLELRQQIGTKLTRRLSQRPTAEELEQRNILKPRNEQEEQEEKREIKRRLTRKL
                     SQRPTVEELRERKILIRFSDYVEVADAQDYDRRADKPWTRLTAADKAAIRKELNEFKS
                     TEMEVHELSRHLTRFHRP"
     exon            271..435
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            436..516
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            517..684
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            685..891
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1213
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1461
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1618
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1674
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1736
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..1877
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..1954
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..5307
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2009..2014
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /note="hexamer: CATAAA"
     polyA_site      2030
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /note="major polyA site"
     regulatory      2098..2103
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /note="hexamer: AAGAAA"
     polyA_site      2099
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /note="major polyA site"
     polyA_site      2123
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
     regulatory      4730..4735
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
                     /note="hexamer: AATAAA"
     polyA_site      4750
                     /gene="Phactr1"
                     /gene_synonym="9630030F18Rik; Rpel1"
ORIGIN      
        1 cccttcccct cctctccttc tcccctcccc tccccctttc tatgtgtgtg agcctcctcc
       61 tttcccctcc tccccctttc cgcctctctc cctccccctc tctccatctc cttctcctct
      121 cagctcgccg ggcgaccctg ctcctgcctc ccacattaat ggcggcttct tcggaggatg
      181 acatagaccg gcggcccatc agaagagtcc gctccaagag cgacacgcca tatctcgcag
      241 aggccaggat ctcctttaac ctgggggcag ccgaggaagt ggagaggctg gcagccatgc
      301 gttccgactc gctggtccca ggtacgcaca ccccgcccat ccggaggagg agtaagtttg
      361 ccaacctggg gaggattttc aaaccctgga aatggaggaa gaagaaaagt gaaaagttca
      421 agcacacgtc agcagctctg gaaaggaaga tttcgatgag gcagagcaga gaggagctga
      481 tcaagagagg ggtcttgaag gagatctacg ataaagatgg agaactctct atatcaaatg
      541 aagatgactc cctggagaac ggacagtccc tgagctccag ccaactctct ttgcctgctc
      601 tgtcggaaat ggagcctgtc ccaatgccca gggacccatg ctcatatgag gtgctccaag
      661 cttcagacat tatggatgga ccagtgtctg aagagagtcc ctctgccagt gagtctggag
      721 tcctcctgtc ccaagatcct tcagccaaac cagttctgtt cctgcccccc aaaaaatctg
      781 ctgctttccc tggagaccat gaggagaccc cagtgaaaca gttgtccctt cacaagcagc
      841 ccccggccct gcctcccaaa cccactgccc ggattgccaa ccacttaaca gatcctggcg
      901 cccctgtgaa attgccttgt ctgccagtga aactgtcgcc tccgctacct ccaaagaaag
      961 tcctgatctg tatgcctgta ggggggccag agctcactct ggcatcctat gcagcccaga
     1021 agagcagcca gcaggccgtg gcccagcacc accacaccgt cctaccatcc cagatgcagc
     1081 accagctgca gtatggcagt cacggccagc atctcccatc ctccaccggc accttgccca
     1141 tgcacccctc aggctgcagg atgatcgacg agctgaacaa gacactcgcc atgacgatgc
     1201 agaggctgga aagctccgag caacgagtcc cctgttccac gtcttaccac agttctggtt
     1261 tgcactcaag cgacggcatc acaaaagcag gacccatggg ccttccggaa ataagacaag
     1321 tgccaactgt tgtgattgaa tgtgatgaca ataaggaaaa tgtgcctcat gaacccgact
     1381 atgaagactc gccctgcctg tatggccggg aggaggagga agaggaggaa gatgaagatg
     1441 atgacgcctc tttgtacacc agctcactgg ccatgaaggt gtgcaggaag gactccttag
     1501 ccatcaaact cagcaacagg ccctctaagc gagagctaga agaaaagaac atcctcccca
     1561 gacagacgga tgaggaacgg ctggagctca gacagcagat tggcaccaag ctcaccaggc
     1621 ggctgagcca gagaccaact gcagaggaac tggaacagag gaacattttg aagcctcgga
     1681 atgaacaaga agaacaggag gagaagcggg aaatcaagag gaggctgact cgcaagctca
     1741 gccaaaggcc cacagtggaa gaactccggg agaggaagat ccttatccgc ttcagtgact
     1801 acgtggaagt ggcagatgcc caagactatg accgaagggc agacaagcca tggacccgcc
     1861 tcaccgctgc agacaaagct gccattcgga aggaactcaa tgagtttaaa agtaccgaga
     1921 tggaagtcca cgagttaagt agacacttaa ccaggtttca tcgaccttaa cagtgggatt
     1981 actcttgagt gctctgctgt cttcaaaaca taaatttcta agaaccataa gtgctggcgt
     2041 ctgtccactt ccccgttacc atgtaaatcc gaactgcctt tttttaaaaa agagagaaag
     2101 aaagagagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag
     2161 gaaaaaggaa gaagaaagga aaggaaacac agccaggatt ctgtgtgcag gaacaccatg
     2221 gtgagcactc ctcagcattg ctggcaccac ttctgctaca gaaaggtgac ccggcccccc
     2281 gagacaggga cctacctgag gattgtccag aaaccgcctt gtcccctcca tccagaccca
     2341 gcaagcacca cattggttca gtcttcggtg aaccccttcc tcacaaggga ctgttgcctg
     2401 agtccaaaga agcccagggc ttgacatggg aatggctagg gtgacagtca taatccaact
     2461 gtccttctag acgtggtcag acacatgggc tccagatggg gcatctgctt tgggggccct
     2521 tgggaagggg gtgacgacct ctcagtctcc tccacacata cacaggcaca cccacgcacg
     2581 aacatgtgca cacacaaaca cacatgcttt ctcttcagat ttgtcctgga ggagcctgtg
     2641 ttggcacttc agtcataccc atgtgtgctg tgggcatagg aggctaaacc tgagcgtcca
     2701 ctttgacccc catcccaaac tgaactgcct tccagaagac tctggtaggg atagtagaca
     2761 tctgctcagt tcacattcat tgaccagttt gtgttcacaa agcccagggc aatgggtgat
     2821 ctaatgcttt aatgcccttg ccaaggttcc ctgtgatatt catgagtgtg aactgaccag
     2881 ggagccatca accccatggg ccacagtttt ctttgaccct gtagcacacc tgggccagtg
     2941 gtgatgtggc ttggtagttg atagcttctg agtaccagtg tatggaggcc agtgcagggg
     3001 accaatatcc cactgtcatt ccagagcctt ccactcttct gtctttgcaa ctgaagagtt
     3061 tctgcagaga gatgcacatc tcagcagggc tagcactggg tcaaactttg ctagccatga
     3121 ctacctgtag tgtcccttac tgccccactt acccagagag accctgctct ctctctctct
     3181 ctctctctct ctctctcaga atatgcctcc aggcaccctg ttgacagggc cccctaaagg
     3241 ttgagtgtgt actgagggaa gggcacccta gatcctaagg cggtcttcta cagggctgtt
     3301 tttctcccta accaagctca cagacccttg ggtgcaaggt ttctgccgca acccccgtgg
     3361 tgtgcccagc tttcggagtc actacggtgt cactaagtaa cacacctggc atttagaaag
     3421 atgccagctg gctagcctga ccaaccacaa aggagttatc cccttgtcaa actggttggg
     3481 accatggtac ttttaggata cagccgaaac aaggccattc aattctccgg agcgttaatg
     3541 gctccgtgac cagttgctaa cccaggaacc agagttacag gaaagcttag attagcccta
     3601 caggtagcgc catgcagctg ctgctatcct ggtaccgtct ggatggggca cacctctgtc
     3661 cagaccaaag acaacaccat gactgggtct ttctgtcatc gctgtcatca tcatcatcat
     3721 tatttgaatg tcaagagagg tattcagggg aaatgtggtg gggattttaa catggctgta
     3781 aatatggtct tcccaaaagg cttactgact tttgaatgtg tacatggtag gaggaaagat
     3841 gcagaaatct ttccttgacc ttccctaggg tgaaggatag gcacatgttc taggtggagg
     3901 tccctgccct gcaggcctga ggtctgaact tcctctaatt agcagctgtg ctgcaggctg
     3961 ctgaggtggc ctggcctgac aggccttccc caaccaggaa gagtcctaaa gaactcctcc
     4021 cacaaaagcc catggcttcc ccaccggcct tggttctagg gcccttctga ttgcctggga
     4081 acagaaatcc caaactcacc tccgtctctg ctgccagcca gcagccttcc ttgttccctg
     4141 ccagcttgcc ctgagatggc ccacctcttt acatatggaa gaaactggac aggatgccca
     4201 catggcttcc ttcggggtac ccttttgcag aagtcacttg ctcagagtta ggggtgtgcc
     4261 tctcatgctc ctaaaaggtt accacctttg gggctgggct atctcaaggc cacgataagt
     4321 ggttcttagc caggctcagc agcctcccca gctgtaggat gggacttctg ctctcctgaa
     4381 aacctgggct caggcttcag ggagctgtaa ctacacactt cccccacttt gtgaggacaa
     4441 ctgaagtgac ttgagtagtt tccaaagttg acaatttgac attaattagg gggaaataga
     4501 aggcaaggga gttggcttga gacatccctt tctggaaaag ctgcagtttt caagtgagtt
     4561 catcttgagc ctagacagtc ccatctgagt accatggagt tcgggttagc aagaaaggag
     4621 aaaactaaat gtgtcagatc ttacgttttt tttttttctc tcaaaatcag gcactgctgg
     4681 acccccaccc ccatccctgt ccgcttcccc gacagaagag cctgccataa ataaactggc
     4741 tcttccaaaa tcctgatcac ttcattcccc agaccccttc aaggccctgc caggttttta
     4801 gaacgggagc ctttctgccc gtagagaaac caaacgactc cagggtcagt gtgaggagat
     4861 actcctagtt tctttttctc ctgcttatga acatgtatgc gtacttttgg aatgagcatc
     4921 gtgatgtgtc actccgatga atgggatccc aggttctccc tgcgcacggc cagtgggcag
     4981 gtgtcacctt tggtgacttc ttcctcccat gagtctctaa gtaggttgct ttctccaatt
     5041 gcttcctggt gtctgtacat agttcgtctt tgtataggag tgattgtggt gaccgtcgct
     5101 cccgcgccct cccgggttct aacttaacag atgacggccg tatgaagaag atactgtaca
     5161 tagactataa atgaaactgg atctctgtgg cctaggtttt gtatatacag aaactgcatg
     5221 gtatttaaat tattgtttct ctctgatgat gtatgcagtt tctttaaaaa caaaccaaaa
     5281 aaaaaaagtt taaaaaaaaa aaaaacaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5341 aaaaaaaaaa
//
