LOCUS       NP_067294                530 aa            linear   ROD 28-APR-2025
DEFINITION  N-acetylmuramoyl-L-alanine amidase isoform a precursor [Mus
            musculus].
ACCESSION   NP_067294 XP_893174 XP_997926
VERSION     NP_067294.2
DBSOURCE    REFSEQ: accession NM_021319.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 530)
  AUTHORS   Basu,P., Das,A.A., Siddiqui,K.N., Mondal,P.C. and Bandyopadhyay,A.
  TITLE     Novel role of peptidoglycan recognition protein 2 in activating
            NOD2-NFkappaB inflammatory axis in coronary artery disease
  JOURNAL   Atherosclerosis 389, 117436 (2024)
   PUBMED   38277990
  REMARK    GeneRIF: Novel role of peptidoglycan recognition protein 2 in
            activating NOD2-NFkappaB inflammatory axis in coronary artery
            disease.
REFERENCE   2  (residues 1 to 530)
  AUTHORS   Dabrowski,A.N., Conrad,C., Behrendt,U., Shrivastav,A., Baal,N.,
            Wienhold,S.M., Hackstein,H., N'Guessan,P.D., Aly,S., Reppe,K.,
            Suttorp,N. and Zahlten,J.
  TITLE     Peptidoglycan Recognition Protein 2 Regulates Neutrophil
            Recruitment Into the Lungs After Streptococcus pneumoniae Infection
  JOURNAL   Front Microbiol 10, 199 (2019)
   PUBMED   30837960
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 530)
  AUTHORS   Arentsen,T., Khalid,R., Qian,Y. and Diaz Heijtz,R.
  TITLE     Sex-dependent alterations in motor and anxiety-like behavior of
            aged bacterial peptidoglycan sensing molecule 2 knockout mice
  JOURNAL   Brain Behav Immun 67, 345-354 (2018)
   PUBMED   28951252
  REMARK    GeneRIF: Study shows sex-dependent alterations in motor and
            anxiety-like behavior of aged bacterial peptidoglycan sensing
            molecule 2 (Pglyrp2) knockout mice. The findings suggest a novel
            role for Pglyrp2 as a key regulator of motor and anxiety-like
            behavior in late life.
REFERENCE   4  (residues 1 to 530)
  AUTHORS   Dziarski,R., Park,S.Y., Kashyap,D.R., Dowd,S.E. and Gupta,D.
  TITLE     Pglyrp-Regulated Gut Microflora Prevotella falsenii,
            Parabacteroides distasonis and Bacteroides eggerthii Enhance and
            Alistipes finegoldii Attenuates Colitis in Mice
  JOURNAL   PLoS One 11 (1), e0146162 (2016)
   PUBMED   26727498
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 530)
  AUTHORS   Gowda,R.N., Redfern,R., Frikeche,J., Pinglay,S., Foster,J.W.,
            Lema,C., Cope,L. and Chakravarti,S.
  TITLE     Functions of Peptidoglycan Recognition Proteins (Pglyrps) at the
            Ocular Surface: Bacterial Keratitis in Gene-Targeted Mice Deficient
            in Pglyrp-2, -3 and -4
  JOURNAL   PLoS One 10 (9), e0137129 (2015)
   PUBMED   26332373
  REMARK    GeneRIF: Pglyrp-2 expression was induced after infection in WT
            mice. Pglyrp-3 expression was low before and after infection in WT
            mice, while Pglyrp-4 expression was slightly elevated after
            infection in WT, Pglyrp-2 and -3 null mice
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 530)
  AUTHORS   Xu,M., Wang,Z. and Locksley,R.M.
  TITLE     Innate immune responses in peptidoglycan recognition protein
            L-deficient mice
  JOURNAL   Mol Cell Biol 24 (18), 7949-7957 (2004)
   PUBMED   15340057
  REMARK    GeneRIF: In contrast to its critical role in immunity in flies,
            PGRP-L is largely dispensable for mammalian immunity against
            bacteria and fungi.
REFERENCE   7  (residues 1 to 530)
  AUTHORS   Gelius,E., Persson,C., Karlsson,J. and Steiner,H.
  TITLE     A mammalian peptidoglycan recognition protein with
            N-acetylmuramoyl-L-alanine amidase activity
  JOURNAL   Biochem Biophys Res Commun 306 (4), 988-994 (2003)
   PUBMED   12821140
REFERENCE   8  (residues 1 to 530)
  AUTHORS   Kibardin,A.V., Mirkina,I.I., Baranova,E.V., Zakeyeva,I.R.,
            Georgiev,G.P. and Kiselev,S.L.
  TITLE     The differentially spliced mouse tagL gene, homolog of tag7/PGRP
            gene family in mammals and Drosophila, can recognize Gram-positive
            and Gram-negative bacterial cell wall independently of T phage
            lysozyme homology domain
  JOURNAL   J Mol Biol 326 (2), 467-474 (2003)
   PUBMED   12559914
REFERENCE   9  (residues 1 to 530)
  AUTHORS   Liu,C., Xu,Z., Gupta,D. and Dziarski,R.
  TITLE     Peptidoglycan recognition proteins: a novel family of four human
            innate immunity pattern recognition molecules
  JOURNAL   J Biol Chem 276 (37), 34686-34694 (2001)
   PUBMED   11461926
REFERENCE   10 (residues 1 to 530)
  AUTHORS   Kibardin,A.V., Mirkina,I.I., Korneeva,E.A., Gnuchev,N.V.,
            Georgiev,G.P. and Kiselev,S.L.
  TITLE     Molecular cloning of a new mouse gene tagL containing a
            lysozyme-like domain
  JOURNAL   Dokl Biochem 372 (1-6), 103-105 (2000)
   PUBMED   10935177
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK050019.1, AY282722.1 and CT485616.11.
            
            On Oct 25, 2007 this sequence version replaced NP_067294.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a). Variants 1 and 2 encode the same isoform (a, also known as
            TagL-alpha).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF149837.1, SRR5189682.143816.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 17.56 cM"
     Protein         1..530
                     /product="N-acetylmuramoyl-L-alanine amidase isoform a
                     precursor"
                     /EC_number="3.5.1.28"
                     /note="N-acetylmuramoyl-L-alanine amidase; peptidoglycan
                     recognition protein long"
                     /calculated_mol_wt=55332
     sig_peptide     1..22
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2393
     mat_peptide     23..530
                     /product="N-acetylmuramoyl-L-alanine amidase.
                     /id=PRO_0000023921"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8VCS0.1)"
                     /calculated_mol_wt=55332
     Site            61
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8VCS0.1)"
     Site            80
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8VCS0.1)"
     Site            174
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8VCS0.1)"
     Site            219
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96PD5; propagated from
                     UniProtKB/Swiss-Prot (Q8VCS0.1)"
     Site            335
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:16944957; propagated from
                     UniProtKB/Swiss-Prot (Q8VCS0.1)"
     Region          360..505
                     /region_name="PGRP"
                     /note="Animal peptidoglycan recognition proteins
                     homologous to Bacteriophage T3 lysozyme; smart00701"
                     /db_xref="CDD:128941"
     Site            order(390,427,502,509,510)
                     /site_type="active"
                     /note="amidase catalytic site [active]"
                     /db_xref="CDD:133475"
     Site            order(390,502,510)
                     /site_type="other"
                     /note="Zn binding residues [ion binding]"
                     /db_xref="CDD:133475"
     Site            order(391..392,423,427,441,448..449,454,502,506,509,510)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:133475"
     Site            427
                     /site_type="other"
                     /note="Important for catalytic activity, essential for
                     amidase activity and zinc hydrate coordination.
                     /evidence=ECO:0000250|UniProtKB:P00806; propagated from
                     UniProtKB/Swiss-Prot (Q8VCS0.1)"
     Site            465
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000250; propagated from
                     UniProtKB/Swiss-Prot (Q8VCS0.1)"
     CDS             1..530
                     /gene="Pglyrp2"
                     /gene_synonym="C730002N09Rik; Pglyrpl; PGRP-L; tagL"
                     /coded_by="NM_021319.5:113..1705"
                     /note="isoform a precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS37557.1"
                     /db_xref="GeneID:57757"
                     /db_xref="MGI:MGI:1928099"
ORIGIN      
        1 mkawgalwiv lglllwpepg aasslpllmd siiqalaele qkvpvteasi tasawilsak
       61 nssthnslhq rlllkapshn ttepdphsls pelqalisev aqhdvqngre ygvvlapdgs
      121 tvavkpllfg leaglqahsv anlpsdclai pcdtgdtlan iratwpglmd afpnasspdv
      181 gatlpndkak tpttvdrlla itlagdlglt flhrsqtwsp pglgtegcwd qltaprvftl
      241 ldpqasrltm aflngaldga llgnhlsqip rphpplshll reyygagvng dpvfrsnfrr
      301 qngaaltsap tlaqqvweal vllqklepeh lqlqnisqeq laqvatlatk efteaflgcp
      361 aihprcrwga apyrghptpl rlplgflyvh htyvpappct tfqscaadmr smqrfhqdvr
      421 kwddigysfv vgsdgylyqg rgwhwvgaht rgynsrgfgv afvgnytgsl pneaalntvr
      481 dalpscaira gllrpdykll ghrqlvlthc pgnalfnllr twphfteven
//
