LOCUS       NM_001162920            3243 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus piggyBac transposable element derived 1 (Pgbd1),
            transcript variant 2, mRNA.
ACCESSION   NM_001162920
VERSION     NM_001162920.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Talluri,T.R., Kumar,D., Glage,S., Garrels,W., Ivics,Z.,
            Debowski,K., Behr,R. and Kues,W.A.
  TITLE     Non-viral reprogramming of fibroblasts into induced pluripotent
            stem cells by Sleeping Beauty and piggyBac transposons
  JOURNAL   Biochem Biophys Res Commun 450 (1), 581-587 (2014)
   PUBMED   24928388
  REMARK    GeneRIF: Thus SB and PB transposons represent a promising non-viral
            approach for iPS cell derivation.
REFERENCE   3  (bases 1 to 3243)
  AUTHORS   Sarkar,A., Sim,C., Hong,Y.S., Hogan,J.R., Fraser,M.J.,
            Robertson,H.M. and Collins,F.H.
  TITLE     Molecular evolutionary analysis of the widespread piggyBac
            transposon family and related 'domesticated' sequences
  JOURNAL   Mol Genet Genomics 270 (2), 173-180 (2003)
   PUBMED   12955498
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY706648.1, BC059882.1 and BC089360.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Both variants 1 and 2 encode the same
            isoform (a), which is longer than isoform b.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC059882.1, SRR1660825.120755.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-9                 BY706648.1         1-9
            10-1367             BC059882.1         1-1358
            1368-3243           BC089360.1         1105-2980
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 7.79 cM"
     gene            1..3243
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /note="piggyBac transposable element derived 1"
                     /db_xref="GeneID:319207"
                     /db_xref="MGI:MGI:2441675"
     exon            1..294
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            295..615
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             481..1728
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /note="isoform a is encoded by transcript variant 2"
                     /codon_start=1
                     /product="piggyBac transposable element-derived protein 1
                     isoform a"
                     /protein_id="NP_001156392.1"
                     /db_xref="CCDS:CCDS49210.1"
                     /db_xref="GeneID:319207"
                     /db_xref="MGI:MGI:2441675"
                     /translation="MLRNFTACDFGTSVTMRQTYPLQRGDEAATLLENLASERGDTGQ
                     QATLCAQEQDMHLVVTEYPGASMEEYQSLHLLQSSVTPWTRDFPELPLGCERSLHLQR
                     SKSAKKSLKTPCYIFDVTSSTTAGNDTELNRDSPSPTEKAVSTLNTVGSCHPPHDSWA
                     KMHIASLQYITQKGREKAKFRVIELLQGHAFSDESETEEDNEPEVQPERKKIKASSIP
                     EKSWTKRDSKPNFPSWSALDSGLLNLKSEKLNPVELFELFFDGETFNLIVNETNNYAS
                     QKNVSLEVTVQEMRCVFGVLLCSGFVRHPRMGMYWEISDSDQTLVRNEIRRDRFELIF
                     SCLHFADNKHLDQKDKFSNLRPLIKQMKNFPLVCPPEEYYCFDKSMCECFDCDQFLNG
                     KPLQIGYKIWCGTTNYARLSGLV"
     exon            616..767
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            768..878
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            879..3228
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2634..2639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2653
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /note="major polyA site"
     regulatory      3199..3204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /note="hexamer: AATAAA"
     regulatory      3203..3208
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
                     /note="hexamer: AACAAA"
     polyA_site      3220
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
     polyA_site      3228
                     /gene="Pgbd1"
                     /gene_synonym="4921509E05Rik"
ORIGIN      
        1 tttggactcc ttcccgccga ccttcatcgt cgcgggctcg gagactaaca gcaagaaccg
       61 ccaggctgtg gcgctctggc ctcttggctc tggtcgcagg cttttttcat gagcttcggc
      121 acatcctcac gtcctcgtag cacacgttcc ctaaactttt aagcagcaaa tagacaccct
      181 tagctgctgg gcgctggcgt atataatttt agcgtagatc tgggcaggtt agagccctgg
      241 cgaaggcact acaggtacat ctattacgtt cgtccggtct ttgttgggta tcagatgttc
      301 atctagaaat gcatttctga aacacagact tcagatagct gagctgttct gcagcttcta
      361 agttcaacat gtatgaagtt ttgctgggtt ctgctcctga gaatgaaggc cgccttgtga
      421 cagtgaaggt ggaagatccc acctggggac agccaggcaa ctcacaggag ggcagggcgc
      481 atgctcagga actttaccgc ctgcgatttc ggcacttctg ttaccatgag gcagacatat
      541 cctctgcaga gaggtgatga agcggcgaca ttactggaga atctagcctc ggaacgtgga
      601 gacacagggc agcaggctac tctctgtgct caggaacagg acatgcacct agtggtgaca
      661 gagtatccag gagcctctat ggaggagtat cagagcctcc atctcctgca gtcttcggtc
      721 actccctgga caagagactt tccagagctc cccctgggat gtgagagatc tctacattta
      781 cagagatcca aatctgctaa aaagagcctc aaaactcctt gttacatctt cgatgttaca
      841 tcctcaacca ctgcaggaaa tgatactgaa ctgaacagag attctccttc ccccactgaa
      901 aaggcagtct ccactttgaa cactgtcggg agctgccacc caccccatga ctcctgggcc
      961 aagatgcaca ttgcgtcctt gcagtacatt actcagaaag ggagggaaaa agccaaattt
     1021 agagtgatcg agctgctcca aggccatgcg ttttctgatg agtcagagac agaggaagac
     1081 aatgagcctg aggtacagcc tgagagaaag aagataaagg catccagcat ccctgagaag
     1141 agctggacca aacgagacag taagcctaac tttccaagct ggtcagccct ggattctgga
     1201 cttttgaatc ttaagagtga gaagttgaac ccagtagagc tctttgaatt attttttgat
     1261 ggtgaaacat tcaacttgat tgtcaatgaa accaataatt atgcttctca gaaaaatgtc
     1321 agcctagaag tcacagtgca ggaaatgagg tgtgtgtttg gtgttctgct ctgcagtgga
     1381 tttgtaaggc atcccagaat ggggatgtat tgggaaatct ctgattctga tcagaccctg
     1441 gttagaaatg aaattagaag ggacagattt gaattaattt tctcatgcct gcattttgca
     1501 gataataagc acctcgatca aaaagataag ttttcaaatt tgaggcctct cataaagcaa
     1561 atgaaaaatt ttcctcttgt atgcccccct gaggaatact attgttttga caagtcaatg
     1621 tgtgaatgct ttgattgtga ccaatttctg aatgggaagc ctcttcaaat tggctataaa
     1681 atttggtgtg gtacaactaa ctacgcaagg ctatctggtt tggtttgagc cttaccaaga
     1741 agactcgggt gtggataagg agctggacct tgggccaggt ggaaatctaa taatgagttt
     1801 tgctgatgtt cttctggaga aaggtcacca tccctatcac ttgagttttg atagcttctt
     1861 tacacctgtt aaactgatgt cagctttgaa gaagaaggga gtgaaggcta caggatcgct
     1921 ttgtgaaaac aaaacaaaaa tgtccactta tgaatgtgga acttatgaaa aagatgaacc
     1981 gggggtattt tgatttcata gtggaagaaa aggaggagat atttttatgc cgttggcatg
     2041 gtgatggctt tattagtctg tgttcaaatg ctgtaggcat agaaccagtc agcaaagcaa
     2101 gctgtgtagc tgacgataag gagttccttc cttcaggtaa catccattgt gaagttatgt
     2161 gaggaatgca ggaaaggtgt agctaaaatg gatcaaatta tttccaaata cagggtggga
     2221 ttaagaagta agaagcggct ctgggttttg gtgagttaca tggtcgatgt agccatgagc
     2281 aatgcgtggc agttgcacag aatctgtaac ccaggctccc ctttagatct cccaggtttc
     2341 cagaaatgtg tggcatgttt tcgcttggga catgatgcta atctgtctga ttaggacgcg
     2401 taaagaacac agtaacaaca cacacacaca aattataagt gtagtaaatc tacattacca
     2461 aagcaaatgt aatggagaag gtgtgattat gtcactggta ttgtttacat caaggattgt
     2521 tacagcgaac aatgctttaa aaataacatt ctgtgagtgt tggatttgtg ttgcttttat
     2581 gtctagtttt gggtcagata tatgaaatga cttacttgtt aaatatttta tatattaaaa
     2641 gagcaaaaac ctaccatttc ctcactttca gttttgtttt agagattgac attttgaaaa
     2701 tgcaggcaat atcacactct atagaatgac actgaattca tagtccagaa tagatacaaa
     2761 acagatatac aactcatgcg ggaggatatg ctaacggata taggtataag ggagtaaata
     2821 tttcagctgg atcacactat atgtatatag ttgagacagg gccaggcatg aaaattgctc
     2881 tgtgtgtgtg tgagtgtgtg tgtgtgtgtg tgtgtagctg tgcctttgaa tactgaagaa
     2941 aatgttatga gttgggagaa gtcagagaga ggaaagtaat gaagcaatct gtgtaacagt
     3001 tatcctgaga agaaagcaag tataaggtga ttggacatag tctagattga ggtcatcagt
     3061 ggttactcat ccaatgagag acatttggag caagtgaact atgactccat aatgctgaag
     3121 atttgtaggg gtaaattaaa ctaaaggcaa ggaggattcg acaccatttc tgtgattatt
     3181 atttaattat ttatataaaa taaacaaaca catctcatta gttctaaaaa aaaaaaaaaa
     3241 aaa
//
