LOCUS       NM_021793               3480 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus post-glycosylphosphatidylinositol attachment to
            proteins 6 (Pgap6), mRNA.
ACCESSION   NM_021793
VERSION     NM_021793.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Lee,G.H., Fujita,M., Takaoka,K., Murakami,Y., Fujihara,Y.,
            Kanzawa,N., Murakami,K.I., Kajikawa,E., Takada,Y., Saito,K.,
            Ikawa,M., Hamada,H., Maeda,Y. and Kinoshita,T.
  TITLE     A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling
            in embryos by shedding CRIPTO
  JOURNAL   J Cell Biol 215 (5), 705-718 (2016)
   PUBMED   27881714
  REMARK    GeneRIF: PGAP6 plays a critical role in Nodal signaling modulation
            through CRIPTO shedding.
REFERENCE   3  (bases 1 to 3480)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   4  (bases 1 to 3480)
  AUTHORS   Motohashi,T., Miyoshi,S., Osawa,M., Eyre,H.J., Sutherland,G.R.,
            Matsuda,Y., Nakamura,Y., Shibuya,A., Iwama,A. and Nakauchi,H.
  TITLE     Molecular cloning and chromosomal mapping of a novel five-span
            transmembrane protein gene, M83
  JOURNAL   Biochem Biophys Res Commun 276 (1), 244-250 (2000)
   PUBMED   11006113
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK044384.1, AC140047.8 and BP766233.1.
            
            On Dec 10, 2009 this sequence version replaced NM_021793.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB045293.1, AK044384.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2596              AK044384.1         2-2597
            2597-2758           AC140047.8         142024-142185
            2759-3480           BP766233.1         1-722               c
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 13.06 cM"
     gene            1..3480
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /note="post-glycosylphosphatidylinositol attachment to
                     proteins 6"
                     /db_xref="GeneID:60455"
                     /db_xref="MGI:MGI:1926283"
     exon            1..252
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..2444
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /EC_number="3.1.1.4"
                     /note="type I transmembrane protein; transmembrane protein
                     8A; transmembrane protein 6; GPI processing phospholipase
                     A2; transmembrane protein 8 (five membrane-spanning
                     domains); ribitol xylosyltransferase 1; post-GPI
                     attachment to proteins factor 6"
                     /codon_start=1
                     /product="post-GPI attachment to proteins factor 6
                     precursor"
                     /protein_id="NP_068565.1"
                     /db_xref="CCDS:CCDS28546.1"
                     /db_xref="GeneID:60455"
                     /db_xref="MGI:MGI:1926283"
                     /translation="MGRVGAGGTAREAATGSLLLLLLLLARPPPAAASNSKESEAGLV
                     SEHFSQAPQKLSFYSWYGSTRLFHFRVPPDTVLLRWLLHVSQGSPSCTDEEITVHFRY
                     GAPPVINPLGTSFPDNTLSHASFHIRALLSTLMLDNTSVNISHPAPGDWFLVAHLPPS
                     SQKIQVKGFVPTCAYIFQPDMLVMRVVEVSTLEPDVPLPQTLLSYPSYLKIFVPEYTQ
                     ELRLELQGCVSSVSPGCPVRVTVGATTLPRNFQRVLTCTGLAPSCHLLLSSPPWGRWL
                     QVTFESLAEPHVTVGFTAKAVFTVCRPWSVTIHHLIQNNPNQTYDTSAIQLSQSAVHR
                     DLGRSSRVDSGPFCLLNYPVLREDTDVVSVHFQPLNGAFVLVHSSMPSVMQLRLDTGM
                     DSGGSFIIVLRTNKTEVTNGTLVAACVNAASPFLSFNTSLNCTTAFFQGYPMFLRASS
                     HMANLIMPFPETDNWYLSLQLVCPESPEDCEQAVVRVETILYLVPCLNDCGPYGQCLL
                     LRRYGYVYAGCSCKAGWRGWSCTDNSTAQTVAQQRAAALLLTLSNLMFLAPIAISLHR
                     SFLVEASVYFYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGASTWVTILCMA
                     RLKTILKQVLLVLGTLVIAMSLQMDRRGIWNLMGPCVFAFVIMASMWIYRCGHRGQCY
                     PTSWQRWVFYLLPGISMASVGIAMYTSMMTSDNYYYTHSIWHILLAGSAAFLLPPREE
                     KAGSWACLQKFPCHYQICRNDRDELYTVT"
     exon            253..427
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            428..635
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            636..763
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            764..1032
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1352
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1443
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1567
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1704
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1883
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2030
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2147
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..3464
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2540..2545
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /note="hexamer: AATAAA"
     polyA_site      2557
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /note="major polyA site"
     regulatory      3446..3451
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /note="hexamer: AATAAT"
     polyA_site      3464
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
ORIGIN      
        1 ggcggcaggt ctatcggagc catgggagct ccgggcagag gcagtggcgg cggccgcaga
       61 ggcggcgggg gctgcccagg cgggggcgag cggcgcggga cgcgggaggg gtagctcggg
      121 ctgagcgccg agccatgggc cgggttgggg ccgggggcac agcgcgggag gcagcgaccg
      181 ggtcactgct gctgctgctg ctgctcctgg ctcggcctcc gcctgccgcc gccagcaaca
      241 gcaaggagag cgaggctgga ctggtatctg aacacttctc ccaggcccca cagaagctgt
      301 ccttctacag ctggtatggc agcacccggc tcttccactt ccgcgtgccc ccggacactg
      361 tgctgctccg ctggctgctg cacgtgtccc agggaagtcc ctcatgcact gacgaagaga
      421 tcacagtgca tttccgctat ggcgcccctc ctgtcatcaa ccctctgggc accagcttcc
      481 ctgacaacac cttgagccac gcctccttcc atatcagggc attgttgagc accttgatgc
      541 tggacaacac ctcagtcaac atctcccacc ctgcccctgg ggactggttc ctggttgctc
      601 acctccctcc ctcctcccag aagatccagg tgaagggctt tgttccaacc tgtgcctaca
      661 tcttccagcc tgatatgctg gtaatgaggg tggttgaagt ctccacccta gagcctgatg
      721 ttcccctccc acagacgctg ctctcctacc ccagctacct caagatcttt gtccctgaat
      781 acacccagga gcttcggttg gaactgcagg gctgtgtgtc cagtgtgagc cccggctgcc
      841 cagtacgtgt cacagtgggg gcaaccaccc tgcctagaaa cttccagagg gtgctgactt
      901 gcactggcct cgctccatcc tgccatttgc tactgtcctc tccaccctgg ggccggtggt
      961 tacaagtgac atttgagagc ctggctgaac ctcatgtgac agtgggcttt actgctaaag
     1021 ctgtctttac agtatgtagg ccatggagtg tgaccatcca tcatcttata caaaacaacc
     1081 caaaccagac ttatgatacc tctgcaatcc agctgtccca aagcgctgtc caccgggacc
     1141 tgggcaggag tagcagggtg gacagtggcc ccttctgtct cctgaactat ccagtcctga
     1201 gggaggacac agatgtggtg tctgtacact tccagcccct gaatggggcc ttcgtgctag
     1261 tgcattcaag catgccttct gtaatgcagc tccgccttga tacaggcatg gacagcggag
     1321 gctccttcat catcgtcctg aggactaaca agacagaggt cacaaatggc accttggtag
     1381 cagcttgtgt gaatgctgcc tcccctttcc tcagcttcaa cacttcactc aactgcacca
     1441 cagccttttt ccagggctac cccatgtttc tgagagcctc atctcacatg gccaacctca
     1501 tcatgccctt cccggagaca gacaactggt acctctccct gcagcttgtg tgccctgaga
     1561 gtcctgagga ttgtgaacag gctgtggttc gcgtggagac catcttgtat ctggtgccct
     1621 gtttgaatga ctgtgggccc tacggccagt gtctcctgct ccgtagatac ggctatgtgt
     1681 acgcaggctg cagctgcaag gcaggttggc gtgggtggag ctgcaccgac aacagcacag
     1741 cgcagaccgt agcccagcag agggcagcag cgcttctgct cacgctcagc aacctcatgt
     1801 tcctggcccc cattgctata tccctgcacc gttccttcct ggtcgaggcc tctgtctact
     1861 tttacaccat gttcttctcc acgttctacc acgcctgtga ccaacccggg gaggcagtgt
     1921 tgtgcatcct cagttatgac acgctacagt attgcgactt tctgggctct ggggcgtcca
     1981 cctgggtgac cattctttgc atggcccgtc tgaagacaat cctgaaacag gtactacttg
     2041 tcctcggcac actcgtcatc gccatgtcct tgcagatgga tcgcaggggc atctggaact
     2101 tgatgggacc ttgcgtcttt gcttttgtga tcatggcctc catgtggata taccgctgtg
     2161 ggcaccgggg acagtgctac ccaacctcct ggcagcgctg ggtcttctac ctcctgcctg
     2221 gcatctccat ggcttccgtt ggcatcgcca tgtatacttc aatgatgacc agtgacaatt
     2281 actactacac ccacagcatt tggcacatcc tactggctgg tagtgcagca tttcttctgc
     2341 caccacgaga ggaaaaagct gggtcctggg cctgtttgca gaagttccct tgtcactacc
     2401 agatctgcag gaatgatcgg gatgagttgt acacagtgac ctgataaggc tggttcatgg
     2461 acacctgaaa ctctaatgac ctcttcagct aggacaagcg agagaggtgc ttgtctagcc
     2521 ccaaccaggt ttccatctga ataaaattgt cctgagacgc ttggcttctt tctccagaga
     2581 agaccccctc caccccaaaa aatctttatg gctgctgcca attcctctga aggtcacagc
     2641 tgtttccacc tggagcccac aggtgccacg tgtgggctga tcacgctgtt atggatgggg
     2701 atacagataa gggattcctg tggtcccctt cctttggaaa gccgtgatat gagacccaca
     2761 agcaaggaag tctgagtgca cagaggtcac tcactgtctc tgacatcctg tacccttaat
     2821 gccatcctgc cttggcatgg ggataccctc ctgctgcagt tgctatggta gagtccaggg
     2881 tcatcagaga gctgattaag acctggagtg ctgtgtgctg tccctccctc attgtctgtt
     2941 ggagcaggag gccttcggga gagcctgagg tagcaggctg ctcagctgta tgaggtctgg
     3001 ctccagaagg cacacaatgc tcgctagtgt tcccttctct cttaaggggt ccaaggcccc
     3061 agtctggtga gcattctcct gtgctggccc tagacacttg gatgaggctg acccctactg
     3121 gatcaactga ggattaagga gtgggttctg gatccccagc ccaatctttc tggtctcctg
     3181 gggtcactga gctggtagtg ctgtgtgccc aggccatttg gttcccactg tgaggctcat
     3241 ctgctctgag tactgggatt gcaggtgtgg gcttctgggc tccttcccaa gctactaaca
     3301 tcctttattt ggcaccaaag cacactttag cccctgaaag gggaaatgtg tatattctgt
     3361 tgggggtggg gggcaggtgg taaaatgtta ctgttctaag atatgaaggc atatttttat
     3421 gtaattggtg aatttaatat tctacaataa tttttcaaat caaaaaaaaa aaaaaaaaaa
//
