LOCUS       NP_068565                769 aa            linear   ROD 28-APR-2025
DEFINITION  post-GPI attachment to proteins factor 6 precursor [Mus musculus].
ACCESSION   NP_068565
VERSION     NP_068565.1
DBSOURCE    REFSEQ: accession NM_021793.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 769)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 769)
  AUTHORS   Lee,G.H., Fujita,M., Takaoka,K., Murakami,Y., Fujihara,Y.,
            Kanzawa,N., Murakami,K.I., Kajikawa,E., Takada,Y., Saito,K.,
            Ikawa,M., Hamada,H., Maeda,Y. and Kinoshita,T.
  TITLE     A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling
            in embryos by shedding CRIPTO
  JOURNAL   J Cell Biol 215 (5), 705-718 (2016)
   PUBMED   27881714
  REMARK    GeneRIF: PGAP6 plays a critical role in Nodal signaling modulation
            through CRIPTO shedding.
REFERENCE   3  (residues 1 to 769)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   4  (residues 1 to 769)
  AUTHORS   Motohashi,T., Miyoshi,S., Osawa,M., Eyre,H.J., Sutherland,G.R.,
            Matsuda,Y., Nakamura,Y., Shibuya,A., Iwama,A. and Nakauchi,H.
  TITLE     Molecular cloning and chromosomal mapping of a novel five-span
            transmembrane protein gene, M83
  JOURNAL   Biochem Biophys Res Commun 276 (1), 244-250 (2000)
   PUBMED   11006113
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK044384.1, AC140047.8 and BP766233.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB045293.1, AK044384.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 13.06 cM"
     Protein         1..769
                     /product="post-GPI attachment to proteins factor 6
                     precursor"
                     /EC_number="3.1.1.4"
                     /note="type I transmembrane protein; transmembrane protein
                     8A; transmembrane protein 6; GPI processing phospholipase
                     A2; transmembrane protein 8 (five membrane-spanning
                     domains); ribitol xylosyltransferase 1; post-GPI
                     attachment to proteins factor 6"
                     /calculated_mol_wt=82161
     sig_peptide     1..33
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=3186
     mat_peptide     34..769
                     /product="Post-GPI attachment to proteins factor 6.
                     /id=PRO_0000022540"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ESN3.1)"
                     /calculated_mol_wt=82161
     Site            138
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9ESN3.1)"
     Site            411
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9ESN3.1)"
     Region          542..725
                     /region_name="DUF3522"
                     /note="Protein of unknown function (DUF3522); pfam12036"
                     /db_xref="CDD:463441"
     Site            544..564
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ESN3.1)"
     Site            568..588
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ESN3.1)"
     Site            604..624
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ESN3.1)"
     Site            628..648
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ESN3.1)"
     Site            652..672
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ESN3.1)"
     Site            689..709
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ESN3.1)"
     Site            716..736
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9ESN3.1)"
     CDS             1..769
                     /gene="Pgap6"
                     /gene_synonym="GPI-PLA2; M83; Rxylt1; Tmem8; Tmem8a"
                     /coded_by="NM_021793.2:135..2444"
                     /db_xref="CCDS:CCDS28546.1"
                     /db_xref="GeneID:60455"
                     /db_xref="MGI:MGI:1926283"
ORIGIN      
        1 mgrvgaggta reaatgslll lllllarppp aaasnskese aglvsehfsq apqklsfysw
       61 ygstrlfhfr vppdtvllrw llhvsqgsps ctdeeitvhf rygappvinp lgtsfpdntl
      121 shasfhiral lstlmldnts vnishpapgd wflvahlpps sqkiqvkgfv ptcayifqpd
      181 mlvmrvvevs tlepdvplpq tllsypsylk ifvpeytqel rlelqgcvss vspgcpvrvt
      241 vgattlprnf qrvltctgla pschlllssp pwgrwlqvtf eslaephvtv gftakavftv
      301 crpwsvtihh liqnnpnqty dtsaiqlsqs avhrdlgrss rvdsgpfcll nypvlredtd
      361 vvsvhfqpln gafvlvhssm psvmqlrldt gmdsggsfii vlrtnktevt ngtlvaacvn
      421 aaspflsfnt slncttaffq gypmflrass hmanlimpfp etdnwylslq lvcpespedc
      481 eqavvrveti lylvpclndc gpygqclllr rygyvyagcs ckagwrgwsc tdnstaqtva
      541 qqraaalllt lsnlmflapi aislhrsflv easvyfytmf fstfyhacdq pgeavlcils
      601 ydtlqycdfl gsgastwvti lcmarlktil kqvllvlgtl viamslqmdr rgiwnlmgpc
      661 vfafvimasm wiyrcghrgq cyptswqrwv fyllpgisma svgiamytsm mtsdnyyyth
      721 siwhillags aafllppree kagswaclqk fpchyqicrn drdelytvt
//
