LOCUS       NM_025944               2989 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus post-GPI attachment to proteins GalNAc transferase 4
            (Pgap4), transcript variant 1, mRNA.
ACCESSION   NM_025944
VERSION     NM_025944.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Hirata,T., Kobayashi,A., Furuse,T., Yamada,I., Tamura,M.,
            Tomita,H., Tokoro,Y., Ninomiya,A., Fujihara,Y., Ikawa,M., Maeda,Y.,
            Murakami,Y., Kizuka,Y. and Kinoshita,T.
  TITLE     Loss of the N-acetylgalactosamine side chain of the GPI-anchor
            impairs bone formation and brain functions and accelerates the
            prion disease pathology
  JOURNAL   J Biol Chem 298 (3), 101720 (2022)
   PUBMED   35151686
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK042679.1 and AK045523.1.
            
            On Sep 20, 2017 this sequence version replaced NM_025944.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK045523.1, AK042679.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2075              AK042679.1         2-2076
            2076-2989           AK045523.1         1924-2837
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 26.63 cM"
     gene            1..2989
                     /gene="Pgap4"
                     /gene_synonym="2810432L12Rik; 9330170P15Rik; Tmem246"
                     /note="post-GPI attachment to proteins GalNAc transferase
                     4"
                     /db_xref="GeneID:67063"
                     /db_xref="MGI:MGI:1914313"
     exon            1..253
                     /gene="Pgap4"
                     /gene_synonym="2810432L12Rik; 9330170P15Rik; Tmem246"
                     /inference="alignment:Splign:2.1.0"
     exon            254..2989
                     /gene="Pgap4"
                     /gene_synonym="2810432L12Rik; 9330170P15Rik; Tmem246"
                     /inference="alignment:Splign:2.1.0"
     CDS             329..1540
                     /gene="Pgap4"
                     /gene_synonym="2810432L12Rik; 9330170P15Rik; Tmem246"
                     /note="transmembrane protein C9orf125 homolog;
                     transmembrane protein 246"
                     /codon_start=1
                     /product="post-GPI attachment to proteins factor 4"
                     /protein_id="NP_080220.2"
                     /db_xref="CCDS:CCDS18177.1"
                     /db_xref="GeneID:67063"
                     /db_xref="MGI:MGI:1914313"
                     /translation="MTTSTSPAAMLLRRLRRLSWGSTAVQLFILTVVTFGLLAPLACH
                     RLLHSYFYLRHWHLNQMSQDFLQQSLKEGEAALHYFEELPSANGSVPIVWQATPRPWL
                     VITIITVDRQPGFHYVLQVVSQFHRLLQQCGPQCEGHQLFLCNVERSVSHFDAKLLSK
                     YVPVANRYEGTEDDYGDDPSTNSFEKEKQDYVYCLESSLQTYNPDYVLMVEDDAIPEE
                     QIFPVLEHLLRARFSEPHLQDALYLKLYHPERLQHYINPEPMRILEWVGVGMLLGPVL
                     TWIYMRFACRPGFSWPVMLFFCLYSMGLVELVGRHYFLELRRLSPSLYSVVPASQCCT
                     PAMLFPAPAARRTLTYLSQVYCHKGFGKDMALYSLLRAKGERAYVVEPNLVKHIGLFS
                     SLRYNFHPSLL"
     regulatory      2057..2062
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgap4"
                     /gene_synonym="2810432L12Rik; 9330170P15Rik; Tmem246"
                     /note="hexamer: AATAAA"
     polyA_site      2075
                     /gene="Pgap4"
                     /gene_synonym="2810432L12Rik; 9330170P15Rik; Tmem246"
                     /note="major polyA site"
     regulatory      2243..2248
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgap4"
                     /gene_synonym="2810432L12Rik; 9330170P15Rik; Tmem246"
                     /note="hexamer: AATACA"
     polyA_site      2262
                     /gene="Pgap4"
                     /gene_synonym="2810432L12Rik; 9330170P15Rik; Tmem246"
ORIGIN      
        1 aacccccacc cccaagccag ctttgggggc tgcagttgct ccgcagctct caggtcacgt
       61 ggcctggcag agcgagggga gccctgcacc ggagcgaatg ctggaggagg cgagccttcc
      121 gctactctcc gcccggcagg gttctgccaa ggcgccacca tcgagaccag ccagcctggg
      181 ctacagcagc ggtggcctgg gctacagcgg cggtggcctt ggcaagccag gacgtagctg
      241 acgcctccac ggggtttgac ttcatgctac tgccctgaga tccaggatgg tttgttcttg
      301 ggaccagaca tgaccaaaag ttgacaccat gaccacgtca acctctccag ctgccatgct
      361 tctccggagg cttcggcgac tctcctgggg cagcacagct gtgcagctct tcattctaac
      421 cgtggtgaca tttggcttac tggccccgct ggcctgccac cggctcctcc actcatattt
      481 ctatctgcgt cattggcatc taaaccagat gagccaggac ttcctgcagc agagcctgaa
      541 ggagggggaa gctgcccttc actactttga agagctgccc tctgccaatg ggtctgtgcc
      601 catcgtctgg caggccactc cccgcccctg gctggtgatt accataatca ctgtggatag
      661 gcagcctggc tttcattatg tcttacaggt agtatcccag ttccatcggc ttctgcagca
      721 gtgcggcccc cagtgtgaag ggcaccagct cttcctgtgc aatgtggaac ggagtgtgag
      781 ccattttgat gctaagctgc tctctaagta cgtccctgtg gccaaccgct acgagggcac
      841 cgaagacgat tacggtgatg acccttccac taactcattt gagaaagaga agcaagacta
      901 tgtgtattgc ctggagtcat cgctgcaaac ctacaatcca gactatgtcc tgatggtgga
      961 ggatgatgct attccagaag aacagatctt cccagtattg gagcacctcc tgcgtgctcg
     1021 cttctctgag ccacacctcc aagatgccct gtatctaaag ctctatcacc cagagaggct
     1081 acagcactac atcaacccag agcctatgcg gatcctggag tgggttggtg tgggcatgct
     1141 gctggggcct gtgctaacct ggatctacat gaggtttgcc tgccgcccag gcttcagctg
     1201 gcctgtcatg cttttcttct gtctctacag catggggctg gtggaactgg tgggccgaca
     1261 ctattttctg gaactgcggc gcctgagtcc ctccttgtac agtgtggttc ctgcctctca
     1321 gtgttgtacc ccggctatgc tcttccctgc ccctgctgcc cgcaggaccc tcacctacct
     1381 gtcccaggtg tactgccaca agggctttgg caaagacatg gcactgtact ctctgctgag
     1441 agccaagggg gagagggcct atgtggtgga gcccaacctt gtgaaacaca ttgggctctt
     1501 ctccagccta cggtacaatt ttcatcccag cctactgtag gagtgccaag aaatgctttt
     1561 ctgctattag ctatttctct gggagaggca cacactggat tctttattta gatgtggttg
     1621 cttgcagata tttcattggt caatgttgct aggtgttgca ggcactggga caggggagga
     1681 atgcatcgag tgaaaagccc tgactgggaa acctccagaa gaagcaggag tttgccacaa
     1741 gttggatctc tctccctcta cacagccaca ctgcctcatg cagtctgcct ctctccgtga
     1801 ggatgaaagt cagtggtagt tatcttctct gtgtctgcaa gttctggctc gtgacagagc
     1861 ccaggactgc caaaaatctt gtgcactgtg ctgcgagact tgaattagga ttttaagggt
     1921 agagtatggt gaacagtcag acgcttttga acaaattcct ttcccacggt tcaagagcgg
     1981 atgtgtgctg agaatctatg cccaaagaag gcaggaccat gttgccatat tctatttgtt
     2041 ttccttggcc tggtgtaata aaagtgtcaa agttgttctg tgtcagttgt cttggaggtg
     2101 gtgatctggc tcttctgaga tacagtgact tcttttccca gtacctgttg tgtattcaga
     2161 gtaaatgcta tggatgaagg ctttaagcac agcagaatct ccgtgcgctt tctgaataac
     2221 ccaagtcggg ttgcttctgt taaatacaca gctaatgcag aacatttctg tgatagtgtg
     2281 attccttggc aggtggatat ttcagaagga gacagaagaa tgacctccat actaggagcg
     2341 tagccttctc cctacatttg tgacctgaag cttcaccctc attccatttt ttactggtga
     2401 cagtgtagca tagaagacca agtatggcct gggaagtgaa aaacctcctg acttgtatat
     2461 tcatggcttc agggagacac gcttgagaga tttttatatc taaaatattt taattgtatg
     2521 tgggaggagg catgtttatg tgtgtggaaa tacgttcctg tatctgtagg ttattacgga
     2581 ggtcagaggt tgacatctgg tgtctttctc agttgttctc tgctgacagg gggcctcact
     2641 gaacgcatca ttcaccagtt tgtgctcgac tggctaagca gcaaactcca gagaatcccc
     2701 tgcctccgct tccctgctac acggttgcag atatgtggca ctctgcctgc ctgctgggga
     2761 acaagctcag atcctcaccc ttctgtggca cgcactgtat caactgagcc atctacctag
     2821 cccacgtttg tacattttag taagatattt gtgtttaatg gggacttagg gcgtgtggct
     2881 ttcaactgaa tagtagctgt ttgagcccaa ccactgctaa aaacagtgac tggtttttca
     2941 ttgtggtttc taattattta cagttcagct tacatctagc cccacctcc
//
