LOCUS       NM_001382592            2000 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus post-GPI attachment to proteins 2 (Pgap2), transcript
            variant 6, mRNA.
ACCESSION   NM_001382592 XM_006507650
VERSION     NM_001382592.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Mucha,M., Skrzypiec,A.E., Kolenchery,J.B., Brambilla,V., Patel,S.,
            Labrador-Ramos,A., Kudla,L., Murrall,K., Skene,N.,
            Dymicka-Piekarska,V., Klejman,A., Przewlocki,R., Mosienko,V. and
            Pawlak,R.
  TITLE     miR-483-5p offsets functional and behavioural effects of stress in
            male mice through synapse-targeted repression of Pgap2 in the
            basolateral amygdala
  JOURNAL   Nat Commun 14 (1), 2134 (2023)
   PUBMED   37185241
  REMARK    GeneRIF: miR-483-5p offsets functional and behavioural effects of
            stress in male mice through synapse-targeted repression of Pgap2 in
            the basolateral amygdala.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2000)
  AUTHORS   Lukacs,M., Roberts,T., Chatuverdi,P. and Stottmann,R.W.
  TITLE     Glycosylphosphatidylinositol biosynthesis and remodeling are
            required for neural tube closure, heart development, and cranial
            neural crest cell survival
  JOURNAL   Elife 8, e45248 (2019)
   PUBMED   31232685
  REMARK    GeneRIF: The authors identified a novel mouse mutant (cleft
            lip/palate, edema and exencephaly; Clpex) with a hypo-morphic
            mutation in Post-Glycophosphatidylinositol Attachment to Proteins-2
            (Pgap2), a component of the GPI biosynthesis pathway. The Clpex
            mutation decreases surface GPI expression. They found that the
            Clpex phenotype is due to apoptosis of neural crest cells (NCCs)
            and the cranial neuroepithelium.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2000)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2000)
  AUTHORS   Perez-Garcia,V., Fineberg,E., Wilson,R., Murray,A., Mazzeo,C.I.,
            Tudor,C., Sienerth,A., White,J.K., Tuck,E., Ryder,E.J., Gleeson,D.,
            Siragher,E., Wardle-Jones,H., Staudt,N., Wali,N., Collins,J.,
            Geyer,S., Busch-Nentwich,E.M., Galli,A., Smith,J.C., Robertson,E.,
            Adams,D.J., Weninger,W.J., Mohun,T. and Hemberger,M.
  TITLE     Placentation defects are highly prevalent in embryonic lethal mouse
            mutants
  JOURNAL   Nature 555 (7697), 463-468 (2018)
   PUBMED   29539633
REFERENCE   6  (bases 1 to 2000)
  AUTHORS   Lee,G.H., Fujita,M., Takaoka,K., Murakami,Y., Fujihara,Y.,
            Kanzawa,N., Murakami,K.I., Kajikawa,E., Takada,Y., Saito,K.,
            Ikawa,M., Hamada,H., Maeda,Y. and Kinoshita,T.
  TITLE     A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling
            in embryos by shedding CRIPTO
  JOURNAL   J Cell Biol 215 (5), 705-718 (2016)
   PUBMED   27881714
REFERENCE   7  (bases 1 to 2000)
  AUTHORS   Stottmann,R.W., Moran,J.L., Turbe-Doan,A., Driver,E., Kelley,M. and
            Beier,D.R.
  TITLE     Focusing forward genetics: a tripartite ENU screen for
            neurodevelopmental mutations in the mouse
  JOURNAL   Genetics 188 (3), 615-624 (2011)
   PUBMED   21515572
REFERENCE   8  (bases 1 to 2000)
  AUTHORS   Ishii,H., Inageta,T., Mimori,K., Saito,T., Sasaki,H., Isobe,M.,
            Mori,M., Croce,C.M., Huebner,K., Ozawa,K. and Furukawa,Y.
  TITLE     Frag1, a homolog of alternative replication factor C subunits,
            links replication stress surveillance with apoptosis
  JOURNAL   Proc Natl Acad Sci U S A 102 (27), 9655-9660 (2005)
   PUBMED   15983387
  REMARK    GeneRIF: The Frag1 signal pathway, by linking replication stress
            surveillance with apoptosis induction, plays a central role in
            determining whether DNA damage is compatible with cell survival or
            whether it requires cell elimination by apoptosis.
REFERENCE   9  (bases 1 to 2000)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC118592.7 and AC139579.3.
            
            On May 17, 2020 this sequence version replaced XM_006507650.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.2318062.1,
                                           SRR7652917.1302651.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AC118592.7         124444-124523
            81-192              AC118592.7         124752-124863
            193-367             AC118592.7         127644-127818
            368-620             AC139579.3         21328-21580
            621-727             AC139579.3         21682-21788
            728-836             AC139579.3         22356-22464
            837-2000            AC139579.3         22635-23798
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7"
     gene            1..2000
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /note="post-GPI attachment to proteins 2"
                     /db_xref="GeneID:233575"
                     /db_xref="MGI:MGI:2385286"
     exon            1..80
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /inference="alignment:Splign:2.1.0"
     exon            81..192
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /inference="alignment:Splign:2.1.0"
     exon            193..367
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /inference="alignment:Splign:2.1.0"
     CDS             203..967
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /note="isoform 2 is encoded by transcript variant 6; FGF
                     receptor activating protein 1; post-GPI attachment to
                     proteins factor 2"
                     /codon_start=1
                     /product="post-GPI attachment to proteins factor 2 isoform
                     2"
                     /protein_id="NP_001369521.1"
                     /db_xref="GeneID:233575"
                     /db_xref="MGI:MGI:2385286"
                     /translation="MYQVPLTLDRDGTLVRLRFTMVALITVCCPLVAFFFCILWSLLF
                     HFKETTSTHCGVPNYLPSVSSAIGGEVPQRYVWRFCIGLHSAPRFLTAFAYWNHYLSC
                     ASPCPGYRLLCRINFSLNVVENLALLVLTYVSSSEDFTIHENAFIVFIAASLGYMLLT
                     CILWRLTKKHTVSQEDRKSYSWKQRLFVINFISFFSALAVYFRHNMYCEAGVYTIFAI
                     LEYTVVLTNMAFHMTAWWDFGNKELLITSQPEEKRF"
     exon            368..620
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..727
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /inference="alignment:Splign:2.1.0"
     exon            728..836
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /inference="alignment:Splign:2.1.0"
     exon            837..2000
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1976..1981
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /note="hexamer: AATAAA"
     polyA_site      2000
                     /gene="Pgap2"
                     /gene_synonym="1810006G21Rik; clpex; Frag1"
                     /note="major polyA site"
ORIGIN      
        1 actcgccccc gggccgggct ggggctcccg gttccgcgcc cctggctacg gggtacggcc
       61 ccctatccac cagtacttgg ggccttttgc tcgagattcc tccaggcact gcagatggag
      121 gcgtggggga gccctggaat ctcagactag ttttgaatcc cagctctgcc agttactagc
      181 ttgtggcttt gagtctgaca agatgtacca ggtcccactg acattggacc gggatgggac
      241 cctagtccgg ctccgcttca ctatggtggc cctgatcact gtctgctgtc cacttgtcgc
      301 cttcttcttc tgcatcctgt ggtccctgct ctttcacttc aaggagacaa catctacaca
      361 ctgtggggtg cccaattacc tgccatcagt gagctccgcc attggtgggg aggtccccca
      421 acgctacgtg tggcgtttct gcattggcct gcactcggca ccccgcttct tgacagcctt
      481 cgcctattgg aaccactacc tcagctgtgc gtctccatgt ccgggttacc gccttctctg
      541 ccgcattaac ttcagtctca atgtggtgga gaacctcgcg ctgctcgtgc tcacctacgt
      601 ctcctcctcc gaggacttca ccatccacga aaatgctttc attgtgttta tcgcggcatc
      661 cctcggttac atgctcctca cctgcattct ctggcggctg accaagaagc acacagtaag
      721 tcaggaggat cgcaagtcct acagctggaa acagcggctc ttcgtcatca acttcatctc
      781 cttcttctcg gcgctggctg tctacttccg gcacaacatg tattgtgagg ctggagtgta
      841 taccatcttt gccatcctgg agtacactgt tgtcctaacc aacatggcgt tccacatgac
      901 agcctggtgg gacttcggga acaaagagct gctaataacc tctcagcctg aggaaaagag
      961 attctaaccc cgtctgtgat gctggaaaac aagaaaccac tctggccttc cctatctcca
     1021 cgttctctct gggccctggg agagaagagc gggaagggta tgacagggct gaccccagca
     1081 ctgctgacct ctccccacca ctcatgggga tcttcaagaa gcatcaccat tcactgaaag
     1141 gtcctaaaaa actgagctgg caggaaagct ccaccacttg gtgctaaaaa aaaaaaaaag
     1201 gtcctgaggt tcactaaggt tagggttcag agcagtggct gctacctgaa gaccgcagcc
     1261 atttctctct ggtcattcat ctgagtgatg tgtctagtga tctaccgtgc acacccagag
     1321 tgtgtgcacc caggtggtag gtccataaat ttgcccaggt gttcaagatg taccctcttc
     1381 cccatcagtt tcattgtgtt cctgtgtgtg tgactgggga aagtacgctc tatgatttct
     1441 aagggctttg tttcttgagg ttatccagtg gaagtagtat tgtgctttgt cttgacagaa
     1501 aatttgtccc agaagaagca tcaagtatag gatatatttt tcttccccat ctaaaacttc
     1561 tagctttttg gaattatgtc tgtaatccca gcatgttgga gggtccatgc caacctgggc
     1621 ttcataatga gaccttgtct caaaaatgaa gtaagatgcc aggtatggtg gcacatgcct
     1681 ttaatcccaa cacagcagag acagtcaaat atctgttgag ttcaagccag cctggtctac
     1741 agttagtttc aggtgagcca gggctatata gtaagacact gttttagaaa aaaaaataat
     1801 aataataatt aaaggaccct aaaatcacag gcttataaga cctttccctc ctttgatcag
     1861 ggggaagggg tcagaggtag atttccctac tccagaatgg gttacttggt tttcctttat
     1921 cttctctact gtcccaggtc cccagctttt atttgtatat tttatttcaa aggacaataa
     1981 atgtttttct tttgccaaca
//
