LOCUS       NM_001163314           10579 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus post-GPI attachment to proteins 1 (Pgap1), mRNA.
ACCESSION   NM_001163314 XM_892623
VERSION     NM_001163314.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Feng,S., Xing,C., Shen,T., Qiao,Y., Wang,R., Chen,J., Liao,J.,
            Lu,Z., Yang,X., Abd-Allah,S.M., Li,J., Jing,N. and Tang,K.
  TITLE     Abnormal Paraventricular Nucleus of Hypothalamus and Growth
            Retardation Associated with Loss of Nuclear Receptor Gene COUP-TFII
  JOURNAL   Sci Rep 7 (1), 5282 (2017)
   PUBMED   28706241
  REMARK    Erratum:[Sci Rep. 2018 May 11;8(1):7629. doi:
            10.1038/s41598-018-25537-y.. PMID: 29752475]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10579)
  AUTHORS   Brommage,R., Liu,J., Hansen,G.M., Kirkpatrick,L.L., Potter,D.G.,
            Sands,A.T., Zambrowicz,B., Powell,D.R. and Vogel,P.
  TITLE     High-throughput screening of mouse gene knockouts identifies
            established and novel skeletal phenotypes
  JOURNAL   Bone Res 2, 14034 (2014)
   PUBMED   26273529
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10579)
  AUTHORS   McKean,D.M. and Niswander,L.
  TITLE     Defects in GPI biosynthesis perturb Cripto signaling during
            forebrain development in two new mouse models of holoprosencephaly
  JOURNAL   Biol Open 1 (9), 874-883 (2012)
   PUBMED   23213481
REFERENCE   5  (bases 1 to 10579)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   6  (bases 1 to 10579)
  AUTHORS   Mu,X., Zhao,S., Pershad,R., Hsieh,T.F., Scarpa,A., Wang,S.W.,
            White,R.A., Beremand,P.D., Thomas,T.L., Gan,L. and Klein,W.H.
  TITLE     Gene expression in the developing mouse retina by EST sequencing
            and microarray analysis
  JOURNAL   Nucleic Acids Res 29 (24), 4983-4993 (2001)
   PUBMED   11812828
REFERENCE   7  (bases 1 to 10579)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   8  (bases 1 to 10579)
  AUTHORS   Zoltewicz,J.S., Plummer,N.W., Lin,M.I. and Peterson,A.S.
  TITLE     oto is a homeotic locus with a role in anteroposterior development
            that is partially redundant with Lim1
  JOURNAL   Development 126 (22), 5085-5095 (1999)
   PUBMED   10529425
REFERENCE   9  (bases 1 to 10579)
  AUTHORS   Juriloff,D.M., Sulik,K.K., Roderick,T.H. and Hogan,B.K.
  TITLE     Genetic and developmental studies of a new mouse mutation that
            produces otocephaly
  JOURNAL   J Craniofac Genet Dev Biol 5 (2), 121-145 (1985)
   PUBMED   4019727
REFERENCE   10 (bases 1 to 10579)
  AUTHORS   Roderick,T.H. and Hawes,N.L.
  TITLE     Two radiation-induced chromosomal inversions in mice (Mus musculus)
  JOURNAL   Proc Natl Acad Sci U S A 67 (2), 961-967 (1970)
   PUBMED   5289032
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK041133.1, AK138171.1, CK392830.1, AC124538.4, CF745447.1,
            CN536101.1, BM250484.2, AK017146.1, BG803764.1, BM943932.1,
            CV558383.1, AK033485.1 and AK141991.1.
            
            On Oct 25, 2011 this sequence version replaced NM_001163314.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.233339.1,
                                           SRR17253012.6464119.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-776               AK041133.1         2-777
            777-1825            AK138171.1         717-1765
            1826-2045           CK392830.1         360-579
            2046-2456           AK138171.1         1986-2396
            2457-2629           AC124538.4         153085-153257       c
            2630-2734           AC124538.4         148467-148571       c
            2735-3019           AC124538.4         147175-147459       c
            3020-3730           CF745447.1         1-711
            3731-3892           CN536101.1         436-597
            3893-4505           BM250484.2         1-613               c
            4506-5215           AK017146.1         533-1242
            5216-5561           BG803764.1         255-600
            5562-5808           AC124538.4         144386-144632       c
            5809-6290           BM943932.1         1-482
            6291-6788           CV558383.1         234-731
            6789-7917           AK033485.1         809-1937
            7918-8238           AK141991.1         306-626
            8239-9383           AK141991.1         628-1772
            9384-10579          AK141991.1         1774-2969
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 27.25 cM"
     gene            1..10579
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /note="post-GPI attachment to proteins 1"
                     /db_xref="GeneID:241062"
                     /db_xref="MGI:MGI:2443342"
     exon            1..251
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..2873
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /note="post-GPI attachment to proteins factor 1"
                     /codon_start=1
                     /product="GPI inositol-deacylase"
                     /protein_id="NP_001156786.1"
                     /db_xref="CCDS:CCDS48260.1"
                     /db_xref="GeneID:241062"
                     /db_xref="MGI:MGI:2443342"
                     /translation="MFLHSVNLWNLAFYVFMVFLATLGLWDVFFGFEENKCSMSYMFE
                     YPEYQKIELPKKLTKRYPAYELYLYGEGSYAEEHKILPLTGIPVLFLPGNAGSYKQVR
                     SIGSIALRKAEDIDFKYHFDFFSVNFNEELVALYGGSLQKQTKFVHECIKAILKLYKG
                     QEFAPTSVAIIGHSMGGLVARALLTLKNFKQDLINLLVTQATPHVAPVMPLDRFITEF
                     YMNVNNYWILNARHINLTTLSVAGGFRDYQVRSGLTFLPKLSHYTSALSVVSSAVPKT
                     WVSTDHLSIVWCKQLQLTTIRAFFDLIDADTKQITQKPKKKLSVLNHHFIRHPAKQFE
                     ENPSIISDLTGTSMWVPVKVSRWSYVAYNESDKIYFAFPLANHRKIYTHAYCQSTMLD
                     TNSWIFGCINSTSMCRQGVDLSWKAELLPTIKSLTLRLQDYPSLSHIVVYVPSVHGSK
                     FVVDCEFFKKEARSMQLPVTHLFSFGLSSRKVTLNTNGLYYNIELLNFGQIYQAFKVN
                     VVSKCTGSKEEITSIYKLHIPWSYEDSLTIAQVPSSTDISLKLHVAQPENDSHVALLK
                     MYTSSDCQYEVTIKTSFPQILGQVVRFHGGALPAYVVSSILLAYGGQLYSLLSTGYCL
                     EYSTILDKEAKPYKVDPFVIMIKFLLGYKWFKELWDAVLLPELDAIVLTSQSMCFPLV
                     SLILFLFGTCTAYWSGLLSSTSVQLLSSLWLALKRPAELPKDIKVMSPDLPVLTVVFL
                     IVSWTTCGALAILLSYLYYVFKVVHLQASLTTFKNNQPVNPKHSRRSEKKSNHHKDSA
                     VQSLRLCANDAEDSLRMHSTVINLLTWVVLLSMPSLIYWLKNLRYYFKLSPDPCKPLA
                     FLLIPAIAILGNTHTVSVKSSKLLKTVSQFPLPLAVGVIAFGSSHLYRVPCFVIIPLV
                     FHALCNFM"
     exon            252..405
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            406..581
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            582..753
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            754..911
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            912..964
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1031
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1137
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1193
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1277
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1324
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..1376
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1454
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1530
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1531..1604
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1656
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1723
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1832
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1833..1871
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1872..1965
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            1966..2056
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            2057..2254
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            2255..2390
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            2391..2441
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2629
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            2630..2734
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     exon            2735..10579
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8011..8016
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /note="hexamer: AATAAA"
     polyA_site      8033
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /note="major polyA site"
     regulatory      10552..10557
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /note="hexamer: AATAAA"
     polyA_site      10572
                     /gene="Pgap1"
                     /gene_synonym="5033403E17Rik; 9030223K07Rik; A530084K22;
                     D230012E17Rik; oto"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcgcgc gctgctcagc tgggcacggc ttggcaccgg agtctttccc tcgctctgtc
       61 taggctgccg agcttctcct ggtgtagagg cgctcgtctg caccatgttt ctccactcag
      121 tcaacctctg gaatctggcg ttctatgtct tcatggtgtt tctggcgacc ctggggctgt
      181 gggatgtctt cttcggcttc gaggaaaaca agtgcagcat gagctacatg ttcgagtacc
      241 ccgagtacca gaaaatagag ctcccaaaga aactgacaaa gcgttatcca gcatatgagt
      301 tatatcttta cggagaagga tcgtacgctg aagaacacaa aattctccct ttgacaggaa
      361 ttcctgttct ctttcttcct ggtaatgctg gaagctataa gcaagttcga tctatcggct
      421 ctattgcact taggaaagca gaagacatcg acttcaagta ccacttcgac ttcttcagtg
      481 tcaacttcaa tgaagaactg gtggcattgt atggtggaag ccttcagaag cagaccaagt
      541 ttgtacacga gtgcattaaa gcgattctca aactctacaa gggtcaagaa tttgctccta
      601 caagtgtggc aataattggt cactcaatgg gtggccttgt tgccagagca ttgcttacac
      661 tgaaaaattt taagcaagat ctgatcaatc ttcttgttac acaagctact cctcatgtgg
      721 cccctgtgat gccattagat cgtttcatta cagagtttta tatgaatgta aacaactatt
      781 ggattttaaa tgctcggcac ataaatttga ctacactttc tgtggctgga ggattccggg
      841 attaccaagt tcgttcagga ctgacttttc taccaaaatt gagccattac accagtgcct
      901 tatctgttgt gagctcagca gtgcctaaga cctgggtctc aacagaccac ctctccatcg
      961 tgtggtgtaa acaattgcag ttgactacaa ttcgagcgtt ctttgatctt attgatgccg
     1021 ataccaaaca aataactcaa aagcctaaga agaaattgtc cgttttgaat caccatttta
     1081 taagacaccc agctaaacag tttgaggaaa atccgtccat aatttctgac ctaacaggga
     1141 catctatgtg ggttccagta aaagtgtcca gatggtccta tgtggcttat aatgaatctg
     1201 ataagattta ctttgcattt cctcttgcaa atcatagaaa aatctacact catgcctatt
     1261 gtcagagcac catgctggat acaaatagtt ggatttttgg ctgcataaac agcacttcca
     1321 tgtgccggca aggggttgat ttgtcatgga aagctgagct gttaccaaca attaagtctc
     1381 tgacgctaag acttcaagac tatccatcct tgtcgcatat tgttgtctat gtgccatctg
     1441 tccacggaag taagtttgtt gtcgactgtg aattctttaa gaaagaagcg agatccatgc
     1501 agctccctgt gactcacctt ttttcctttg gactgtcttc caggaaggtg acgttaaata
     1561 caaacggcct atactacaac atagagctgc tgaactttgg acagatatac caagctttta
     1621 aagtcaacgt ggtcagcaag tgcactggaa gcaaagaaga aataaccagt atctataaac
     1681 ttcatatccc ctggtcctat gaagattcac taaccatcgc acaggttcca tcttccacag
     1741 acatttctct gaagcttcat gttgctcagc cagaaaatga cagccatgtg gcactgttaa
     1801 aaatgtacac gtcgtcagac tgtcagtatg aggtgacaat taagacttcc ttcccacaaa
     1861 tacttggaca ggtagttagg tttcatggtg gagcccttcc tgcctacgtt gtatctagca
     1921 tccttcttgc gtatggagga cagttatact cactcctctc aacaggttat tgcttagaat
     1981 attctaccat attggataaa gaagccaaac catacaaagt tgacccattt gtaattatga
     2041 ttaagttttt gttggggtat aaatggttta aggaattatg ggatgcagtg ttattaccgg
     2101 aattagatgc catcgttttg actagccaga gtatgtgttt ccctcttgta tccttgattc
     2161 tctttctgtt tggaacatgt actgcatact ggagtggtct actctcttct acatctgtgc
     2221 agcttctttc ttcattgtgg ctagctctga aaagacctgc tgaacttcct aaagatatca
     2281 aggtgatgtc accagacttg cctgttttga cagttgtctt tctcatagtg agttggacaa
     2341 cttgtggagc acttgccata cttctgtctt atctgtacta tgtgtttaag gttgttcatc
     2401 tgcaagccag cctaactaca tttaaaaata atcagccagt gaatcccaaa cactctagaa
     2461 gaagtgaaaa aaagtccaac caccataaag actctgctgt acaaagtctt cgcttatgtg
     2521 ctaatgatgc tgaagacagt cttcgcatgc acagcaccgt gattaacttg ctgacgtggg
     2581 ttgtgctgct cagcatgccg tctctaatct actggttaaa gaaccttagg tattatttta
     2641 aacttagtcc tgacccatgc aaacccctgg catttctcct tattccagct atagcgattc
     2701 tcggaaacac tcacactgtt tcagtaaagt ccagtaaatt gctgaagacc gtctcccagt
     2761 tcccacttcc tctggctgtt ggcgtgattg cttttggctc ctcccactta tacagggtcc
     2821 cgtgttttgt gatcattcct cttgtgttcc atgcgttatg caactttatg taagactgga
     2881 aggagaagcg gctcatgcct tttggaccag tgacacagta cagcgccacc tccaggaaca
     2941 gtgaggtcct ctggagaaaa caagtctatt tttacagtat tgaaaatagg aaattagttt
     3001 tgtaatgctc gagggacgta gttgaagcat ggtttcagcc cgtggtgtag catcggtata
     3061 cttgtcgtat ctgacagatt agtgaaggga tgctgtggcc tctgtcttaa ggactcctgt
     3121 acataccaac tagctttcat ctgccatccc aggaaccttc taaagactat agatgcagtg
     3181 ctgtgtgcag tctaagtgaa acttctgcta gtttcatcag actttgtttt gaaaagtggg
     3241 ttttatattt gttataattt acatattcaa aataagtctt aaaagattgt tagaagtgac
     3301 gactgttttc tcagaagtaa aatagtcatg gagtctggtc agtttttggc agccatggtg
     3361 ggcaaagttt gatccatcat cttacatctg tgaaaaagag cctccaatta aagcgagggt
     3421 tttttgttaa ccgctgaagt tcagattcaa aacaatggca ttttcatgaa ctttcaaata
     3481 tgaatcttac agtgtatact tggtgaatat tagaagcatg gaagagatct atcaaaagat
     3541 ctattaagaa aagaatgttc ttgcagattc tgcattggca cagtcagcct ccgcccctga
     3601 cttaaatcct tgccccagtg gttgccagaa gtgcctgtag caatgatagg ttgttaaagg
     3661 gataactcca gaggccagaa caactctggg tttgcatcat ttgtttgtct actgaggagg
     3721 aagggtctca gcataaatac aacattgtac ttgatgtaca aagctatata ctaatagaga
     3781 aacaatggac agcctttact gaaaatgtga acactgcctg aatagattgc acacctgtat
     3841 cactcattaa gagcaaaatc ttggtttgta gaggtgtctc ctaagactac aattttgcca
     3901 ctggggggag cgggggtaac attttatttc atttccaatt atagttcatc cttagctata
     3961 gaaaaatagg aaggtcagga gcttgaagaa tatagttgtt ctttgccttc agttagaata
     4021 aattcaagct gagtcagacc tgtgtcgtca aacccttcac ttagatgtgt gtcctataaa
     4081 tgcccataag cctttcccgt gaagtcctcc agtgttacgg aattcacagt tgcacttgtg
     4141 aacttatgtc cttctcagtg cccaggaatg gtactaagag aatcagcttt gttttatcgg
     4201 ctgttacaca cggtgtcaca gcctctcagc tgctactgcc aaccagaggg ttctgcccac
     4261 gtcagccatg ttagttcttc cctggacctt tgcttcttca gagctaatca aagggtcagg
     4321 ttattaccac agaagttagt tggtttgtat gattctgtca ctgtctgact ggcatcgtgc
     4381 ttgttaaata ttcatatttt cacttattaa gacttttaaa ggaaagccat cgtactagac
     4441 atggcagacc ctttagtccc ttgtttcaga agagtgtcaa aaggaataaa ctgtaccata
     4501 gtgtttgttg agtacccaag agtgagttca ttggataatt acaattttct catggcacaa
     4561 cacttacata tctaagtagg aatgtatctg ctctaacttt gctggagatg tgtggcctct
     4621 ctacacatgc tgtctttcat tctgtcagtg gtattataga cgtagctcaa tttcatggca
     4681 actgaatact tcaactttct gaatttgaac atatatatac acacagaaag aactttccaa
     4741 agtttggtgt tagacttttt gacagccatg attatttatt tatgagatct aaagtctccc
     4801 tgcttagcca gtattgctca tgaatgggtt gtatcaagtt tgattttacg agtagtatat
     4861 ttgttattaa agcacattaa tactaacaac aatatttttg tctgaaagta ctaattccct
     4921 aagcccttaa agtcttattt atgccagcaa aatatttcaa ataatagtag cctgggtctt
     4981 cagccatttg ccacacatac tcagagatac acactcacac ataaacacag atgggcatgc
     5041 tttgatagaa tcttcatcaa gcatttagac acagaaagct aggtagacta tctgattagg
     5101 aaaagtgtta tcccttaaaa ggaaatgatt tcatataaac atcacatact tttgtctatc
     5161 atgtttacat gaatataggt tatggtagag attaatacag caaatttttg agttctattg
     5221 tcatgttttt gtgttttttt ttaagaaatt gagttttggg gtaaatgtac ttatttaaaa
     5281 ctgggttctt gcttgtaaat agattgggta tatatcagac agttcatctg tctgattatt
     5341 gaatgcttag tattttttaa atgccctcat agattattgc tattataatt tagacctgat
     5401 catttttttg aagagtgatt gtgtgtgtgt gtgtgtgtgt gtacatatgt gtgctggtgc
     5461 tcattgtggc cagcagaggg cggttagagg tgggttgtga attgctggat atcaatacta
     5521 ggaaccaaac tcaggtcctc cagaagagca agcactctga acgaagcccc ctctccagcc
     5581 cctggacctg ataaataggt agcatttgct attgcagttc tgtgtctatg tgaatgagta
     5641 tagtagggat tgaatccgga gcctgtgtat gttagttatg ttctctacta ttgagtaaca
     5701 cacagcttgt ctggattcat tccatgccaa aattaagtaa attttactct tttcttggtg
     5761 tcactataat gaaaacttct aaagaaacca tttatgctta attaacaccg atttttgagg
     5821 ttgccctatg tctatattga tcatgtattg attagtcttt taaatctttt tatattttag
     5881 tattgtaatt tcagctgtct atgaagggat actttttatt gagtgatctt tataattatc
     5941 acacatgtca atatgacaca ttttcacaat gctcaccaac tcatacaagg ctgattgtct
     6001 ttctatcaag tccctgtaca ttttttttaa gccttttact cttttcactt atagcataat
     6061 gtgttcattt caccatgcac tctcttagtc cctcctatgg gagatactga agtagaggga
     6121 agcagaactc agtttttagt tcttgaaagc cgcaggaggc atatgagaag taggcctgga
     6181 gagatggttc agacagtcac atgcctgcgg tgcaagcatg aggacccaag tgtagactgc
     6241 cagagtctat gtgagaaact ggctaactaa tgcatgcctg caatctcagc actggggcaa
     6301 tggagacaga tagatctttg gggcttgctg gctggccagc ctggccatat tggcaagctt
     6361 caggattggt gaaagatttt atgtcaaaaa aatgaggtgg aaagtgattg aggaaaacac
     6421 ccagcattga cctctccctg caccttcata tatacacatg ggtgcacata cgtgaacatg
     6481 taaacacaca cacacacaca caaatactca agaggtgggg atccacaaat atgaagtaaa
     6541 ccagagcagt tgattccata actgtggaat gagtctggtg cctcctgtct ccccttagac
     6601 aggtctgaat taccttgctt gatttggttt gatgcaaact tttagaatgc tgtatttaag
     6661 catataagaa gtggttctaa aaaatcattt ggaaaaaaat ccaaaagtat gttgatgtgt
     6721 tggatagcta ggtttttgtt gattgttttt catctgtgtg gaagatttgt gagtttgtat
     6781 atcataggaa acaagagact ataaagttta tttagggtca cggtccattt ggggagtgtg
     6841 tcacttgcca gaataaaggg aaccatggga gtttctgggt ttaagacatt acttccttcc
     6901 gggaaatctt ctttaccaga taaagtaaat gtagaactca ccctacaaac tgtaaggttg
     6961 acagctagaa ttttactgca gactttttga acaagaaaca taattctgtg aatatctact
     7021 ctttcaccat aaggcttgct gatatgtgag gaaacgagaa agctctgggc tgttcacact
     7081 cctaactttt cagagtgtta tggaaaatgg gagtcaggtc agcagtccga atgtgtgcta
     7141 taaatgctta aacttctgca attgtaaaac ataccacgaa taaataaaaa acagaatttt
     7201 atatgttggt aacttggttt tgagaacaga gtaagatttg gaaatgctga tgctaggcat
     7261 gttgttactc tgttaatacc ataaatgtag tagtgcagct aaggtgacca tgttcgaagt
     7321 tggcactatc taacctacag cttgattaac gtgtgcgaat aataatttta aaatcgagaa
     7381 cagtttttat tttcagtgct tgttatgtaa tgctgccaaa aaacgtcctt atactacgaa
     7441 gccagttaat ttctgatgac ttgatgtaga gttggtatct cgagtcagac tcaacctgta
     7501 agcattctgt cattgttgac ttgaacatca gtgggaaatg ctatctattg gtgtttagtt
     7561 accgtaaact ctgcatatct gaattctaat cttagtctaa gaagcctgtg gggattttgt
     7621 aaagttcatg tgctatttta aatgttttta cttttagtat ttgtaaggta gtgatgttac
     7681 tatgacaaaa gtttatagac actgtgtagc actactaact gctccagagc caaacagaca
     7741 gtgtattttg tatttcatgt gtactttttc tgtttcatat tggatttttt acatcccttt
     7801 tttaggggaa aaaaaaaagg atacatttaa attcttttta gggtcatctg tattggaaac
     7861 taagtattct caaggttagc aaacatactt taccaccaaa gctgaatgaa actgtaagat
     7921 acctctggag atttgtgcca atgaactttt cttagtaatt tggggttaaa acaaaagtaa
     7981 tcttttcagt atttttcatc caggacttac aataaatgtg gatatcatga aaacatgttc
     8041 catactctgt ttttctttga aaattttcaa agatttagaa cagtgtgttg ttctgtgttc
     8101 ttttgtgtct ggtttcctga cattgctagc taaaaggtga atccacttag tagtaatctt
     8161 ttagcagtta attgcggtaa taatcatttg aagtaatctg accgtggctt gttttgtggt
     8221 gtaaaaaaaa aaaaaaaatc agttgttttg cacaatccat ttttggtgta gatgtctggt
     8281 tactacagtt gaaaccttcc ctgtggtgtt tcttttgctt tctttatgtc tctgctcagt
     8341 ccccagtgtt catccactag aacctaatat agtataaggc aaaccagtta gcatgcatat
     8401 cttgtgccaa atactctgaa atacagttgt taatgaaaaa ccctggcatt ggtacggtgt
     8461 tagataaacc tagaactata gccacagagc agtcacgaga atgctagttc ctcctcagag
     8521 gagcggcatg ggaattgcag tgttccttta gtttgcttag catctactgc ttctttgccc
     8581 tacagtactg tgatgttggt acatttttca taagttagcc tcacaattaa agagacagca
     8641 cagaaaacca gatattgaaa tattcggtct tgatcgagta atgattatct aagtaactgt
     8701 tggggactgt ggataaatta gcaggagggt agtttatgag agctggcttc aggacgctct
     8761 gtcctctgga aggagtttgt cttctgtgtg cagtgatctt agcttaggcg ggagatgcct
     8821 gtcccttaac ataagcaggc tctgtgctta tgaaggcaga ggtttgtgca gatctccgtc
     8881 ccctgctgtg tggttgggaa agaaatcttc atttttattg tgtataaatt aggaagttgt
     8941 gctgcatact ttttttgaat atttttttga aaataggcac tgacgtgtat ccttttcaag
     9001 tgatgctgtg gttttttaaa cgtaggagga actataaacg tagcaacatg cccaagataa
     9061 accaaaactg agtatacccc atcactgtga ccttgaactt atttgggata aaacagactg
     9121 tgccttaaaa tgacaaagca atgccctgca gtcttgtaac tgtattttac tgtgggtggt
     9181 cttctagagg ttgtctgaaa cccctttctt tcaagttgtc gagataaaag ttttaagtac
     9241 caatggaccc acatttactt tagtatccct tagctgctgt tcctgaggcc gcccttaatt
     9301 tccagagctt tttgttttga ttttattttt atttaaaaat tgacttagtc taaatatatg
     9361 tgagaaatta gatttggcta aagtttctgg gggaaaatat tttaagggta gtcagggcac
     9421 ttctgcttct aaactcgctg aatatgaaaa gattaatcac tgaaatgtat cttagtctta
     9481 ttgataataa tcctatcaaa gatgtgcatt ccctctgaga cttgtggtca gtgttgtggc
     9541 agatcaagat gcgtttatat tttcaaataa gggttattag gtttatagta aggatttccc
     9601 agtgactaaa ctccaatgaa tgtagctctt gttttgagca ctaccgtttc actttccttg
     9661 ttctttgtcc tgcctcatta gctttatggt attaaggggg cattagctcc tctgttttta
     9721 atttaatgta aacttttata tttgtatgtg ttgatttaaa ataatgagta taatttccat
     9781 taaagtccaa attactcatt ttatagtata atcatattct ttgcccaaag atgctcattt
     9841 ctgggaactc agtctgttgt ttatttttac atgttacttt tgggcataaa ctcaaatgtt
     9901 tatgcctcct agttcacctc cattacaatt acgattttat atttaaataa gagtcttaaa
     9961 aatatgtaga aatgacaata tgtctttttt ttttaagcct ggtcagattg acaaagaagt
    10021 tgtgcaagca gtgggtgtct agtgagatca gtttctacgg ttgagcgttg agagcacaca
    10081 ctcctcatgg ctgctgaggg agactgtgta cagagatcat ttatttgtat gctaatggtt
    10141 atattcttat ccataaaatg tagtgtagta acaatgcgtc atgaagggag cctgtcctga
    10201 cgtgagcagt gagaacggtc tcggtgcaga tctccctgtc tcaacatgca tgcttgtccc
    10261 agtgctgtgt gccatgtgca gtgcactaat acacctctgt gtacttttct gtgtattttt
    10321 ggtatggtat aattaactgg ttatgttaca tttaactggt tgtgattcgg gggggaaagt
    10381 gctacttatt ctaatttgta gcgtgtcatg aatggtttta cctgtgtttc agcattgtga
    10441 tgtggtttta ttatgtgaat atttgccttc agtctccgct caggtattaa cgtgtagcga
    10501 tttttggtgc tttgttttta atttgtcata actggtgttc tgaatgttca aaataaattt
    10561 ggtgaataag cacatttgg
//
