LOCUS       NM_019540               3897 bp    mRNA    linear   ROD 07-AUG-2023
DEFINITION  Mus musculus pore forming protein-like (Pfpl), mRNA.
ACCESSION   NM_019540
VERSION     NM_019540.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Mould A, Morgan MA, Li L, Bikoff EK and Robertson EJ.
  TITLE     Blimp1/Prdm1 governs terminal differentiation of endovascular
            trophoblast giant cells and defines multipotent progenitors in the
            developing placenta
  JOURNAL   Genes Dev 26 (18), 2063-2074 (2012)
   PUBMED   22987638
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Ouseph MM, Li J, Chen HZ, Pecot T, Wenzel P, Thompson JC, Comstock
            G, Chokshi V, Byrne M, Forde B, Chong JL, Huang K, Machiraju R, de
            Bruin A and Leone G.
  TITLE     Atypical E2F repressors and activators coordinate placental
            development
  JOURNAL   Dev Cell 22 (4), 849-862 (2012)
   PUBMED   22516201
REFERENCE   3  (bases 1 to 3897)
  AUTHORS   Piao Y, Ko NT, Lim MK and Ko MS.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   4  (bases 1 to 3897)
  AUTHORS   Hemberger M, Himmelbauer H, Ruschmann J, Zeitz C and Fundele R.
  TITLE     cDNA subtraction cloning reveals novel genes whose temporal and
            spatial expression indicates association with trophoblast invasion
  JOURNAL   Dev Biol 222 (1), 158-169 (2000)
   PUBMED   10885754
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129773.4.
            
            On Feb 28, 2022 this sequence version replaced NM_019540.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052215.1, AK141262.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-123               AC129773.4         201389-201511
            124-3897            AC129773.4         201800-205573
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 8.72 cM"
     gene            1..3897
                     /gene="Pfpl"
                     /gene_synonym="Epcs5; Epcs50"
                     /note="pore forming protein-like"
                     /db_xref="GeneID:56093"
                     /db_xref="MGI:MGI:1860266"
     exon            1..123
                     /gene="Pfpl"
                     /gene_synonym="Epcs5; Epcs50"
                     /inference="alignment:Splign:2.1.0"
     exon            124..3897
                     /gene="Pfpl"
                     /gene_synonym="Epcs5; Epcs50"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..2282
                     /gene="Pfpl"
                     /gene_synonym="Epcs5; Epcs50"
                     /note="ectoplacental cone, invasive trophoblast giant
                     cells, extraembryonic ectoderm and chorion sequence 50"
                     /codon_start=1
                     /product="pore forming protein-like precursor"
                     /protein_id="NP_062413.2"
                     /db_xref="CCDS:CCDS50397.1"
                     /db_xref="GeneID:56093"
                     /db_xref="MGI:MGI:1860266"
                     /translation="MNSFIVTVLIWTTVAYAEEEKALKEIHEYGFQKCQNALNLPVLE
                     VLPGGGWDNLRNMDMGQVMDLTYNNCKTTEDGQYIIPDDVFTIPQKESNLEMNSEILD
                     SWENYRSTTSFSINLNLDYRPRVNGKFSSEFQRIKTTQVRDQAVTTRAQVRNLVYTVK
                     ANPNAELNLGFKKELMEICDRLEKNQTKMATYLAELLILNYGTHIITSVDAGATLVQE
                     DHVKSSYLKNNKGNRAAVAASAGFTFAKVVNFKIETGFEYQNNLATGYLQNRTGSRVQ
                     SIGGIPFYPGMTIETWQKGIINHLVAVDRAGLPLHFFIKPEKLPAFPRHLVEQLAKTV
                     ETAAESYYNFNTYPGCTNINSPNFDFQANTDDGSCDGKLVNAPFGGVYQLCRQLSGHD
                     YDDMCLDFHQKNPLTGDFSCPPDYTPVHLLSQTHEKGYTGMECRDKCILKIFCRTSCK
                     DEFRVAKAEVSSYWCAASSQVPENSGILFGGVFTDKSINPMTNEPSCPTSYFPLKLFE
                     NVKVCVSLDHELGPKYSVPFGGFFSCTKGNPLYGSFTSGDLEKSFLPKCPGGFSQQLA
                     AIIDGCQVSYCVKAGVFTRASLAPARLPPYTQLPMSQLSTDSVEMTGESPISWVKDPY
                     TLQWNLEESSKSGRLSGGSTAAITIVVILALGVLTAMAIYGNRRFKKNKPNGLPRQQA
                     LLPFPRVRVFPDGVQDPNPA"
ORIGIN      
        1 gcgacggcgt cttcattttc ggcaaaagaa tctggcactg gagactgaca agagaacaga
       61 acagtgagcc aagattgtaa caagcctgag agagtgaaga aagcactaca gcagactctt
      121 caggctctct ggtggatgtt taggactggt gtgtgtctgg gactacctgc accatgaaca
      181 gcttcattgt cacagtcctt atctggacca cagtggcata tgctgaagag gagaaggcac
      241 ttaaagagat ccatgagtat ggtttccaaa agtgccagaa tgctctgaat ctacctgttt
      301 tggaagtgct acctggagga ggctgggata atctgaggaa tatggacatg gggcaagtga
      361 tggacttgac atacaacaac tgtaagacca cagaagacgg acagtacatc atccctgatg
      421 atgtctttac tattcctcag aaagagagca acctggagat gaactcagaa atcctggatt
      481 cctgggagaa ttaccgaagc actacttcct tttccattaa cttgaatctc gattatcgtc
      541 ccagagtcaa cggcaagttc tcttctgagt tccaaaggat aaagaccact caagtgagag
      601 accaggctgt aaccacacga gctcaggtga gaaatttggt ctacacagtg aaagccaacc
      661 caaatgcaga actcaacttg gggtttaaga aggagctcat ggaaatctgt gaccgtctag
      721 agaaaaacca gacgaagatg gccacctacc tggcagaact cttgatcctc aactatggca
      781 cacacataat cactagtgtg gatgctgggg ctacactggt tcaggaggat catgtaaagt
      841 cctcctatct taagaacaat aagggaaacc gtgctgctgt ggctgcatct gctggattta
      901 ctttcgcaaa ggttgtgaac ttcaaaatag agacaggctt tgagtatcaa aataacttgg
      961 ccacaggcta tcttcaaaac agaaccggtt ctagggttca gagtattgga ggaattccct
     1021 tctacccagg gatgaccatc gaaacctggc agaagggcat cattaaccac ctggtggcag
     1081 tggaccgtgc tggcttgcct ctgcatttct ttatcaaacc tgaaaagctt cctgcttttc
     1141 cacgccactt ggtggagcaa ctggcaaaga cagtggaaac tgctgcggaa agctattaca
     1201 actttaatac atacccaggc tgcacaaata tcaactcccc caactttgat tttcaagcca
     1261 atacagatga tggctcttgt gatggtaaac tggtcaacgc tccctttgga ggggtttatc
     1321 agttgtgccg tcaattatca gggcatgatt acgatgacat gtgcctagat ttccatcaga
     1381 agaatccact tactggtgat ttctcttgtc cgcctgacta cacccctgtc catctgctat
     1441 ctcagaccca tgagaagggt tatactggaa tggaatgtag agataaatgt atcctcaaga
     1501 tattctgtcg tacatcgtgt aaagatgagt tcagagtggc caaggctgaa gttagctctt
     1561 attggtgtgc ggccagtagc caagtaccag aaaactcagg aattctcttt ggaggagtct
     1621 tcactgacaa gagcatcaac cctatgacaa atgaaccatc gtgcccaacc agttactttc
     1681 ctctgaagct gtttgagaac gtcaaggtat gtgtttctct ggatcatgag ttggggccca
     1741 aatattcagt cccctttggt gggtttttta gctgcacaaa agggaacccc ttgtatggtt
     1801 ctttcacatc tggagactta gagaaatcat ttctgccaaa gtgtcccggg ggcttcagcc
     1861 aacagctcgc tgcgatcatt gatggatgcc aagtgtccta ctgtgtcaag gctggagtct
     1921 ttacacgagc atctctggct cctgctaggc tcccacctta cactcagcta cctatgagtc
     1981 agctttccac tgactcagtt gagatgacag gggagagtcc catatcctgg gttaaggacc
     2041 cctataccct gcaatggaac ctagaggagt catcaaaaag tggtagacta tcaggaggat
     2101 caactgctgc aatcacaatt gtggttatcc tagcattagg agttttaact gctatggcca
     2161 tctatggcaa ccggaggttt aagaagaata aacccaatgg acttccaaga caacaggcat
     2221 tgcttccttt tccaagagtt agagtctttc ctgatggagt gcaggatcca aatccagctt
     2281 aattcttctt taaagaaaca gttcctctca tctccagaca caggttcaag ctcctctttc
     2341 agtcctttct cctctacctc tgccttccta agagctaaag cagaagtcgc aaccgaaaga
     2401 aggtttttta taactttgtg gttatgttta tgactataca ttgggaatta aaggatctgt
     2461 gcaaataagg caggggaagg aaaacttgca acccagtcag caaaaacaag cccaggttct
     2521 cagccattgt cggaatgctc attcccacac aagccactgg tgtcttcacc tgaatctctg
     2581 catgaaagaa caactgggtg gagagcaaca agaactttgg gatgaagctg ctcttattgt
     2641 gaagttggca aaggttttcc aatttgtgag cagaacactt tgccttccct gtatcttgtt
     2701 ttgtatttta gcctcttaaa gacaacccaa actctgagaa catgcagatg gtccagttca
     2761 accagtgttg tggagactcc aaaactctgt actgatgact gcgtcatctc cctgtgtgcc
     2821 tgaagggcac agagttcaga acaagtccac tttctgtctg ctgcacagtt agaatttacc
     2881 ttgttcaacc tacttttaga gcaattttaa cttgtgaact tcccatgcaa tgtccaaggc
     2941 aaaagggtgg tggggaactg gaaataaatc aagaaatgta aaaactgaag aggactccga
     3001 tgttttagga acttgctggt ttcttttagc caactgatgt ctacctttga gctcatcata
     3061 tagtggacag ggaaagggac tgttgttgag atggatcaat tattatggtt tagttttcca
     3121 tgttaccaaa agtataaccc tttctctttc tgcaactagc actcaccctt aatgtcttcc
     3181 catcagaagt gtgaatacag atacaaagtc acatgaatag atacaccctt attacagcaa
     3241 gaagtattta ttttcctaga agtgaaagat gtcaaaatca atataatccc acatttaact
     3301 attattttct ccctaaaact aatgccaaac agaagacatc atgtcctgtc catttaggtt
     3361 tgcttgtttt cactgctaca aagagggctg atgtgataaa aagttgcatc attaagttag
     3421 ctggaacaga cacccaaaat cacctgtgac atgcatttca gatccaggat ggcttccatg
     3481 gggcatccag caggctgcat gactgacacc tggcaatagc tgggttttgc tcaattttta
     3541 tatctaagaa tttaattctt tacaacatga aaattcctct acagctgctt tagaaattat
     3601 gtatctattt accatagcac atcatcagtg agctatcttc tatgaaaaac gatgtgttct
     3661 ctcctttatg ctaacaatca agggcttgtg cacatagtag accctcctga agagctccat
     3721 gtgtctcagc ctctgggatt aggggaaaaa ttattcattt gttttcagct gagatgtgga
     3781 gaggagtgaa tagctgagtt tgaagtctgc cctactggct atccacccaa ggggttgatt
     3841 gtatatgctc ttcacaaaac ctgccttttt cagtattaaa atgttcagta ttaagac
//
