LOCUS       NM_028376               1870 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus profilin family, member 4 (Pfn4), transcript variant
            1, mRNA.
ACCESSION   NM_028376 XM_356571
VERSION     NM_028376.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Umer,N., Phadke,S., Shakeri,F., Arevalo,L., Lohanadan,K.,
            Kirfel,G., Sylvester,M., Buness,A. and Schorle,H.
  TITLE     PFN4 is required for manchette development and acrosome biogenesis
            during mouse spermiogenesis
  JOURNAL   Development 149 (16) (2022)
   PUBMED   35950913
  REMARK    GeneRIF: PFN4 is required for manchette development and acrosome
            biogenesis during mouse spermiogenesis.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT025683.13, BC094614.1 and BI135172.1.
            
            On Mar 2, 2018 this sequence version replaced NM_028376.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK013595.1, BC116888.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-975               CT025683.13        118118-119092
            976-1113            CT025683.13        123215-123352
            1114-1219           CT025683.13        124243-124348
            1220-1306           CT025683.13        127003-127089
            1307-1848           BC094614.1         4234-4775
            1849-1870           BI135172.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 2.09 cM"
     gene            1..1870
                     /gene="Pfn4"
                     /gene_synonym="2900024P18Rik"
                     /note="profilin family, member 4"
                     /db_xref="GeneID:382562"
                     /db_xref="MGI:MGI:1920121"
     exon            1..975
                     /gene="Pfn4"
                     /gene_synonym="2900024P18Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             859..1248
                     /gene="Pfn4"
                     /gene_synonym="2900024P18Rik"
                     /note="profilin IV"
                     /codon_start=1
                     /product="profilin-4"
                     /protein_id="NP_082652.1"
                     /db_xref="CCDS:CCDS25790.1"
                     /db_xref="GeneID:382562"
                     /db_xref="MGI:MGI:1920121"
                     /translation="MSHLQNLLLDTLLGTKHVDSAALIKLQEKTLCVTSPGFSVMPSD
                     VRTLLNGFAKNPLLTRREGLYFKEKDYKCVRADDYSLYAKNENTGVVVVKTNMYLVVA
                     TYTAGMYPSVCVEATEKLGEYLRKKGN"
     exon            976..1113
                     /gene="Pfn4"
                     /gene_synonym="2900024P18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1114..1219
                     /gene="Pfn4"
                     /gene_synonym="2900024P18Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1853
                     /gene="Pfn4"
                     /gene_synonym="2900024P18Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1831..1836
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfn4"
                     /gene_synonym="2900024P18Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      1848
                     /gene="Pfn4"
                     /gene_synonym="2900024P18Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtgctacgcc cgcccctagc ggagcctcgt ctcctcttgt agcccctact ctgtgtttag
       61 ttcccggcct ccaggactca ggattcagtt aggctaccgg cctcccatca attagtcctc
      121 cgaaggcagc ggcgggagag agtcgttcgc cttactagga acctgcggca aaagagtgtt
      181 tgtggcggtt gaccctttga gggccgtcca agatggcagc acatgagcag taaatgtagc
      241 tctaaagagc ctccaaggtg gtcttagagc tcaggtttgt tgaccggtgt cttgtctaca
      301 gcgttgatgc tggccaagga ggaggaatga aaagagtatc ttttttaaat cggtggtgca
      361 ccttgagtgg ttttgagatt ttgtggataa ctggttgctt ttttttgttt ttactttatt
      421 ttacttttat ttatttgttt gttcattttt gcggagctga gaattgaatg gaggacttat
      481 tcgcagatct ttatttttct aagacagggt ctctttggcc ttgaacttgt gaccttcctg
      541 cccttagagt acccaaaata ataggcatag acctttgttt ttaaatctca ggtaaatcat
      601 taaatagata aaaatattga ggctctgatt tttttttcaa actcaccttc ataaaccatt
      661 ctgtgaattc tgatttttct ataatagttt ttcttcccct ctgagtactg gtgaaccctg
      721 gatgatggac cactattttc actcgtccac cgttttagag gacacacact gataataagc
      781 tggaacgtac acaagaagca cttgtattgt ccctcgtgga gtcttcagct gcaggttctg
      841 gttcagagcc gaagaagcat gagtcacttg cagaacttac tgttagatac cctgctgggg
      901 acgaaacacg tggacagcgc agccctcatc aaactccagg aaaagaccct gtgtgtgaca
      961 tcaccaggat ttagtgtaat gccaagtgat gttcgaacac ttctgaatgg atttgccaaa
     1021 aacccattat taacgcgaag ggaagggttg tatttcaagg agaaggatta caaatgtgtt
     1081 cgggcagatg actattctct ctatgctaag aatgagaaca ctggtgtggt ggtggtgaag
     1141 accaacatgt atcttgtggt ggcaacttac actgcaggca tgtatcctag cgtctgcgtg
     1201 gaagccacag agaagctggg agagtatcta aggaaaaagg gaaactaaga ccgaagataa
     1261 ctagtgaaga caacttggtt gttttagaag aaaagtatag atccttaaga aaaaatattt
     1321 tgtggttgaa aacactgcat ggaagattaa aacaaagact gagctaaggg aaaaagtttt
     1381 cttatttcgg aagagcatcc tctctaactg gaaggacctg accagctttt cccttttctt
     1441 taagctttgg tcacagacat gacacctgag tgcatttatg gtttttccca gggcaagtct
     1501 cctgggagtc ttatctccaa gtccccacac atcttgccag atatgccaag aatataggcc
     1561 ctagccactc atatgtgtga accccattca catcgaagtc agagggcagc cataggagtc
     1621 acctctcctc tgggtgatgg gagtggaacg caggccataa ggcttggtgg caagtaaacc
     1681 catgagtcat ctcactgctt ccgggaacca ctctgtagct agggccatgc cagtctcatg
     1741 cctccttaaa ggcctaacgt aatacttgga gggaaaactc gctaagtgct ttctgtcaaa
     1801 gatgtagagt tcctgattca gtgccccttt attaaaatat agaccacagt gcaaaaaaaa
     1861 aaaaaaaaaa
//
