LOCUS       NM_001163487            2894 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phosphofructokinase, muscle (Pfkm), transcript variant
            1, mRNA.
ACCESSION   NM_001163487
VERSION     NM_001163487.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ayyangar,U., Karkhanis,A., Tay,H., Afandi,A.F.B., Bhattacharjee,O.,
            Ks,L., Lee,S.H., Chan,J. and Raghavan,S.
  TITLE     Metabolic rewiring of macrophages by epidermal-derived lactate
            promotes sterile inflammation in the murine skin
  JOURNAL   EMBO J 43 (7), 1113-1134 (2024)
   PUBMED   38418556
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2894)
  AUTHORS   Marinelli,I., Parekh,V., Fletcher,P., Thompson,B., Ren,J., Tang,X.,
            Saunders,T.L., Ha,J., Sherman,A., Bertram,R. and Satin,L.S.
  TITLE     Slow oscillations persist in pancreatic beta cells lacking
            phosphofructokinase M
  JOURNAL   Biophys J 121 (5), 692-704 (2022)
   PUBMED   35131294
  REMARK    GeneRIF: Slow oscillations persist in pancreatic beta cells lacking
            phosphofructokinase M.
REFERENCE   4  (bases 1 to 2894)
  AUTHORS   Gao,L., Ruan,Z. and Chen,G.
  TITLE     MicroRNA-383-5p Regulates Oxidative Stress in Mice with Acute
            Myocardial Infarction through the AMPK Signaling Pathway via PFKM
  JOURNAL   Dis Markers 2021, 8587535 (2021)
   PUBMED   34917202
  REMARK    GeneRIF: MicroRNA-383-5p Regulates Oxidative Stress in Mice with
            Acute Myocardial Infarction through the AMPK Signaling Pathway via
            PFKM.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2894)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2894)
  AUTHORS   Nakajima,H., Noguchi,T., Hamaguchi,T., Tomita,K., Hanafusa,T.,
            Kono,N., Tanaka,T., Kuwajima,M. and Matsuzawa,Y.
  TITLE     Expression of mouse phosphofructokinase-M gene alternative
            transcripts: evidence for the conserved two-promoter system
  JOURNAL   Biochem J 303 (Pt 2) (Pt 2), 449-453 (1994)
   PUBMED   7980403
REFERENCE   7  (bases 1 to 2894)
  AUTHORS   Yamasaki,T., Nakajima,H., Kono,N., Hotta,K., Yamada,K., Imai,E.,
            Kuwajima,M., Noguchi,T., Tanaka,T. and Tarui,S.
  TITLE     Structure of the entire human muscle phosphofructokinase-encoding
            gene: a two-promoter system
  JOURNAL   Gene 104 (2), 277-282 (1991)
   PUBMED   1833270
REFERENCE   8  (bases 1 to 2894)
  AUTHORS   Gekakis,N., Gehnrich,S.C. and Sul,H.S.
  TITLE     Phosphofructokinase isozyme expression during myoblast
            differentiation
  JOURNAL   J Biol Chem 264 (7), 3658-3661 (1989)
   PUBMED   2521854
REFERENCE   9  (bases 1 to 2894)
  AUTHORS   Nakajima,H., Noguchi,T., Yamasaki,T., Kono,N., Tanaka,T. and
            Tarui,S.
  TITLE     Cloning of human muscle phosphofructokinase cDNA
  JOURNAL   FEBS Lett 223 (1), 113-116 (1987)
   PUBMED   2822475
REFERENCE   10 (bases 1 to 2894)
  AUTHORS   Barbehenn,E.K., Wales,R.G. and Lowry,O.H.
  TITLE     The explanation for the blockade of glycolysis in early mouse
            embryos
  JOURNAL   Proc Natl Acad Sci U S A 71 (4), 1056-1060 (1974)
   PUBMED   4275392
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI930496.1, CX218942.1, AK002711.2 and AA420165.1.
            
            Transcript Variant: This variant (1) represents the predominant
            transcript. Variants 1, 2, 3 and 4 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK002711.2, AK049773.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AI930496.1         6-51
            47-708              CX218942.1         39-700
            709-2874            AK002711.2         612-2777
            2875-2894           AA420165.1         462-481
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 54.09 cM"
     gene            1..2894
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /note="phosphofructokinase, muscle"
                     /db_xref="GeneID:18642"
                     /db_xref="MGI:MGI:97548"
     exon            1..141
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            142..234
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..2492
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /EC_number="2.7.1.11"
                     /note="phosphofructokinase, polypeptide X;
                     phosphofructokinase-1 A isozyme; 6-phosphofructokinase,
                     muscle type; phosphofructokinase-M (connecting peptide);
                     phosphohexokinase; phosphofructokinase M variant 3/4;
                     6-phosphofructokinase type A; phosphofructokinase 1;
                     phosphofructo-1-kinase isozyme A; phosphofructokinase 4"
                     /codon_start=1
                     /product="ATP-dependent 6-phosphofructokinase, muscle
                     type"
                     /protein_id="NP_001156959.1"
                     /db_xref="CCDS:CCDS27786.1"
                     /db_xref="GeneID:18642"
                     /db_xref="MGI:MGI:97548"
                     /translation="MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFT
                     GARVFFVHEGYQGLVDGGEHIREATWESVSMMLQLGGTVIGSARCKDFREREGRLRAA
                     HNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQKDGKITAEEATKSSYLNIVG
                     LVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGRHCGYLALV
                     TSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITS
                     EDIKNLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPAC
                     VVSLSGNQAVRLPLMECVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVR
                     PPVSKGGLHTVAVMNVGAPAAGMNAAVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEE
                     AGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNIQGLVIIGGFEAYTGGLEL
                     MEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSAAGTKRR
                     VFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGL
                     VLRNEKCNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAK
                     AMNWMSGKIKESYRNGRIFANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQ
                     WWLKLRPILKILAKYEIDLDTSDHAHLEHISRKRSGEAAV"
     exon            235..308
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            309..386
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            387..576
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            577..742
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            743..787
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            788..896
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            897..992
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1085
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1211
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1276
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1340
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1490
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1561
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1649
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1802
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1967
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..2029
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            2030..2141
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..2241
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            2242..2347
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     exon            2348..2880
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2857..2862
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /note="hexamer: AATAAA"
     polyA_site      2880
                     /gene="Pfkm"
                     /gene_synonym="ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka;
                     Pfkx"
                     /note="major polyA site"
ORIGIN      
        1 ggcctgtaga ggtttggaag cctctcctcc tcccgcggct gcctccacct ggctcctccc
       61 cgtgttcgcc aagctcccct ccccctttcc ccaaggacaa tctgcaagaa agcagcggcg
      121 gaggagagct aaaactacaa gagtggatca tgacccatga agagcatcat gcagccaaaa
      181 ccctggggat cggcaaggcc atcgccgtgt tgacctctgg tggagatgcc caaggtatga
      241 atgctgccgt cagggctgtg gtccgagttg gtatcttcac gggcgcccgc gtcttctttg
      301 tccatgaggg ttaccaaggc ctggtggacg gtggcgagca catcagggag gccacgtggg
      361 agagcgtgtc tatgatgctt cagctgggtg gcacagtgat tggaagtgcc cgatgcaagg
      421 acttccgaga gcgagaagga cgactccggg ccgcccacaa cctggtgaag cgggggatca
      481 ccaatctgtg tgtcatcgga ggcgatggca gcctcactgg ggctgacact ttccgttcag
      541 agtggagcga cttgctgaat gatctccaga aagacgggaa gatcacagcc gaggaggcta
      601 caaagtccag ctacctgaac atcgtgggcc tggttggctc aatcgacaat gacttctgtg
      661 gcactgatat gaccattggt accgactctg ccctgcacag gattgtggag atcgtagacg
      721 ccatcaccac cacggctcag agccaccaga ggacgtttgt gttagaagtg atgggccgcc
      781 actgtggata cctggccctt gtcacctctc tgtcctgtgg ggccgactgg gtttttattc
      841 ctgagtgtcc gccagacgat gactgggaag aacacctttg tcgccggctc agtgagacaa
      901 ggacccgtgg ctctcgtctc aacatcatca ttgttgctga aggtgccatc gacaagaacg
      961 ggaagccaat cacctcagaa gacatcaaga acctggtggt gaagcgcctt ggatatgaca
     1021 cccgggtcac tgttctggga catgtacagc ggggtgggac accatcagcc tttgaccgga
     1081 tcctgggcag caggatgggt gtggaagcag tgatggcact tttggagggg accccagaca
     1141 ccccagcctg tgtggtgagc ctctctggta accaggctgt gcgtttgccc ctcatggagt
     1201 gcgtgcaggt gaccaaagac gtgaccaagg ctatggatga gaagagattt gatgaagcca
     1261 ttaagctgag aggccggagc ttcatgaaca actgggaggt atacaagctt ctagctcatg
     1321 tcagaccccc agtctctaag ggtgggttgc acacagtggc cgtgatgaat gtgggggccc
     1381 cagctgctgg catgaatgcc gctgttcgct ctaccgtgag gattggcctt atccagggca
     1441 accgggtgct ggtcgtacat gatggctttg agggtctggc caaaggtcag attgaggagg
     1501 ctggctggag ctatgttgga ggctggactg gtcaaggtgg ttccaaactt ggtactaaaa
     1561 ggactctccc caagaagaac ctggaacaga tcagtgccaa cataaccaag tttaacatcc
     1621 agggccttgt catcattggg ggctttgagg cttacacagg gggcttggag ctgatggaag
     1681 gcaggaagca gtttgatgag ctctgcatcc cgtttgtggt cattccggcc acggtttcca
     1741 ataacgtccc tgggtcagac ttcagcatcg gggctgacac agcactgaac accatctgca
     1801 cgacttgtga ccgaatcaag cagtctgcag caggcaccaa acgtcgggtg tttatcatcg
     1861 agactatggg tggctactgt ggctacctgg ccaccatggc agggctggcc gctggggctg
     1921 atgctgccta catttttgag gaacccttca ccattcgcga tctccaggtg aatgttgaac
     1981 atctggtgca gaagatgaaa acaactgtga agagaggcct ggtgctgagg aatgagaaat
     2041 gcaatgagaa ctacactact gacttcattt tcaacctgta ctctgaggag gggaagggca
     2101 tctttgatag caggaagaac gtgcttggcc acatgcagca gggtggaagc ccaactccct
     2161 ttgacaggaa ttttgccact aagatgggtg ctaaggctat gaactggatg tctgggaaaa
     2221 tcaaggagag ttaccgtaat ggacggatct ttgccaacac ccctgactca ggctgcgttc
     2281 tggggatgcg taagagggcc ctggtctttc agccagtaac tgagctgaag gaccagacag
     2341 actttgaaca ccgaatcccc aaagaacagt ggtggctgaa gctgaggcca atcctcaaaa
     2401 tcctagccaa gtacgagatt gatctggaca cctccgacca cgcccacctg gagcacattt
     2461 caaggaaacg gtctggagaa gccgccgtct agacctctct ggagtgaggg ggaaagatca
     2521 tgtgaccatg gtcagctcac accctggtag atcatggctt ctcagtgttc tagcccagcc
     2581 cctccccttc taggtttccc tgtactccat gcctgcaacc aggatcagct gtggccagga
     2641 gctggaggag gggcggtgag tgactctcct aggtggctgt ccttcctccc ttccactcca
     2701 gcttcatcgg tcacacaggc tgggtctctc tagtgctacg gctagatttc agctactcaa
     2761 gagttttcct aaaaataagc tttatttatt tctttgtgat aacaaagagt cttaggtccc
     2821 tttactcgca ccacagtgat gaagtgtaac tacactaata aatgccagct ggccactgtg
     2881 aaaaaaaaaa aaaa
//
