LOCUS       NM_173019               3377 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
            (Pfkfb4), transcript variant 1, mRNA.
ACCESSION   NM_173019
VERSION     NM_173019.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Wang,S., Bei,Y., Tian,Q., He,J., Wang,R., Wang,Q., Sun,L., Ke,J.,
            Xie,C. and Shen,P.
  TITLE     PFKFB4 facilitates palbociclib resistance in oestrogen
            receptor-positive breast cancer by enhancing stemness
  JOURNAL   Cell Prolif 56 (1), e13337 (2023)
   PUBMED   36127291
  REMARK    GeneRIF: PFKFB4 facilitates palbociclib resistance in oestrogen
            receptor-positive breast cancer by enhancing stemness.
REFERENCE   3  (bases 1 to 3377)
  AUTHORS   Dai,T., Rosario,S.R., Katsuta,E., Sawant Dessai,A., Paterson,E.J.,
            Novickis,A.T., Cortes Gomez,E., Zhu,B., Liu,S., Wang,H.,
            Abrams,S.I., Seshadri,M., Bshara,W. and Dasgupta,S.
  TITLE     Hypoxic activation of PFKFB4 in breast tumor microenvironment
            shapes metabolic and cellular plasticity to accentuate metastatic
            competence
  JOURNAL   Cell Rep 41 (10), 111756 (2022)
   PUBMED   36476868
  REMARK    GeneRIF: Hypoxic activation of PFKFB4 in breast tumor
            microenvironment shapes metabolic and cellular plasticity to
            accentuate metastatic competence.
REFERENCE   4  (bases 1 to 3377)
  AUTHORS   Chen,Y., Wu,J., Zhang,S., Gao,W., Liao,Z., Zhou,T., Li,Y., Su,D.,
            Liu,H., Yang,X., Su,P. and Xu,C.
  TITLE     Hnrnpk maintains chondrocytes survival and function during growth
            plate development via regulating Hif1alpha-glycolysis axis
  JOURNAL   Cell Death Dis 13 (9), 803 (2022)
   PUBMED   36127325
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3377)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3377)
  AUTHORS   Chesney,J., Clark,J., Lanceta,L., Trent,J.O. and Telang,S.
  TITLE     Targeting the sugar metabolism of tumors with a first-in-class
            6-phosphofructo-2-kinase (PFKFB4) inhibitor
  JOURNAL   Oncotarget 6 (20), 18001-18011 (2015)
   PUBMED   26221874
  REMARK    GeneRIF: Inhibition of PFKFB4 suppresses glycolysis and
            proliferation of multiple human cancer cell lines.
REFERENCE   7  (bases 1 to 3377)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 3377)
  AUTHORS   Leite,T.C., Coelho,R.G., Da Silva,D., Coelho,W.S.,
            Marinho-Carvalho,M.M. and Sola-Penna,M.
  TITLE     Lactate downregulates the glycolytic enzymes hexokinase and
            phosphofructokinase in diverse tissues from mice
  JOURNAL   FEBS Lett 585 (1), 92-98 (2011)
   PUBMED   21074528
REFERENCE   9  (bases 1 to 3377)
  AUTHORS   Minchenko,O.H., Ogura,T., Opentanova,I.L., Minchenko,D.O. and
            Esumi,H.
  TITLE     Splice isoform of
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4: expression
            and hypoxic regulation
  JOURNAL   Mol Cell Biochem 280 (1-2), 227-234 (2005)
   PUBMED   16311927
REFERENCE   10 (bases 1 to 3377)
  AUTHORS   Minchenko,O., Opentanova,I. and Caro,J.
  TITLE     Hypoxic regulation of the
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene family
            (PFKFB-1-4) expression in vivo
  JOURNAL   FEBS Lett 554 (3), 264-270 (2003)
   PUBMED   14623077
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CA316033.1, AY646092.2 and BC057594.1.
            
            [WARNING] On Jul 19, 2024 this sequence was replaced by
            NM_173019.6.
            
            On Aug 3, 2006 this sequence version replaced NM_173019.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK155172.1, AY646092.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-211               CA316033.1         1-211
            212-1848            AY646092.2         98-1734
            1849-3377           BC057594.1         1801-3329
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 59.63 cM"
     gene            1..3377
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     4"
                     /db_xref="GeneID:270198"
                     /db_xref="MGI:MGI:2687284"
     exon            1..211
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     CDS             115..1524
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="PFK/FBPase 4; 6PF-2-K/Fru-2,6-P2ase 4;
                     6PF-2-K/Fru-2,6-P2ase testis-type isozyme"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 4"
                     /protein_id="NP_766607.3"
                     /db_xref="CCDS:CCDS23541.1"
                     /db_xref="GeneID:270198"
                     /db_xref="MGI:MGI:2687284"
                     /translation="MASPRELTQNPLKKIWMPYSNGRPALHASQRGVCMTNCPTLIVM
                     VGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDIVKTYKSFEFFLPDNEEGLKI
                     RKQCALAALSDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVESICV
                     DPEVVAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKI
                     MDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSP
                     RGREFSKHLAQFISDQNIKDLKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEE
                     MTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQ
                     AVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQ
                     NVDISRPSEEALVTVPAHQ"
     exon            212..328
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            329..425
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            426..492
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            493..567
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            568..624
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            625..746
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            747..954
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1101
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1206
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            1207..1336
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1399
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1464
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..3361
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3322..3327
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /note="hexamer: AATAAA"
     regulatory      3338..3343
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /note="hexamer: AACAAA"
     regulatory      3343..3348
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /note="hexamer: AACAAA"
     polyA_site      3344
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
                     /note="major polyA site"
     polyA_site      3358
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
     polyA_site      3361
                     /gene="Pfkfb4"
                     /gene_synonym="C230090D14"
ORIGIN      
        1 agagttggag ccgctagagg gtgggcgggg ccggaggggc gggacctcgg gtggcccgag
       61 gtggcccggt tgctgggtga gtagcagcgc agtccgattc atcccagccc agggatggcg
      121 tctccacggg aactgaccca gaatcccctg aagaagatct ggatgccgta cagcaatggc
      181 aggcccgctc tgcacgccag ccagcgtggt gtctgcatga ctaactgtcc gacactcatt
      241 gtcatggtgg gcctgccagc ccggggcaag acctacattt ctaagaagct gacgcggtac
      301 ctcaactgga ttggcgtgcc tactcgggaa ttcaacgttg gtcagtatcg ccgggacata
      361 gtcaagacgt acaaatcttt tgagtttttc ctcccagaca atgaagaagg cctgaaaatc
      421 aggaagcagt gtgccttagc agccctcagt gatgtccgga agttcctcag tgaggagggg
      481 ggacatgtgg cggtttttga tgcaaccaat acgacccgag agcggagagc gatgatcttt
      541 aactttggag aacagaatgg ctacaagacc ttctttgttg aatctatctg tgtggatcct
      601 gaggttgttg ctgccaacat cgtgcaagtg aagctgggca gccctgacta tgtcaaccgg
      661 gacagcgacg aagccaccga agacttcatg aggcgcattg aatgctatga aaactcatat
      721 gagtcgctgg atgaagacct ggacagggat ctgtcctaca tcaagatcat ggacgtgggc
      781 cagagctatg tggtgaaccg tgttgctgat cacatccaga gtcgcatcgt ttattacctc
      841 atgaacatcc atgtgacacc ccgctccatc tacctctgcc ggcatgggga gagcgagcta
      901 aacctcaagg gccggattgg tggggatcct ggactgtccc cccggggccg ggagttttcc
      961 aagcatctgg cccagttcat cagtgaccag aacattaagg atctgaaggt ctggacgagc
     1021 cagatgaaga ggacgatcca gacagccgag gcgctgagcg tcccttatga gcagtggaag
     1081 gtcctcaacg agatcgatgc gggcgtctgt gaggaaatga cctacgaaga aatccaggat
     1141 cactacccgc tggagtttgc cctgcgggat caggacaagt accggtaccg gtacccgaag
     1201 ggtgagtcct atgaagacct ggtgcagcgg ctggagcccg tcatcatgga actggagagg
     1261 caggagaatg tgttggtcat ttgccaccag gctgtcatgc gctgcctcct ggcctacttc
     1321 cttgacaagg cagctgaaga gctgccctac ctcaaatgcc ccttgcacac agtcctgaag
     1381 ctcacacccg tggcttacgg ttgtaaagtg gagtccatat tcctgaacgt ggcagctgtg
     1441 aacactcacc gagacagacc tcagaatgta gacatatcca ggccttcaga ggaagccctt
     1501 gtcacagtcc ctgctcacca gtgaccctga cctctgtcag aggggtcact ccaggccatc
     1561 ttgtgtttgc tgtactgatg gtgccaccat gcaggaacac tcctgaagcc atgggtggct
     1621 agcaacactc agaaccttca gcctggccca ccggcctctt tgttgaccac cagtgacagg
     1681 ttgggtatgg ctgctgagaa tccctgggct atgtgctctg ctgccagccc ctctgctaca
     1741 gcactcgagc agtccactca ccaggctggc tgcttcatga cgtttgcaac cttaaatgag
     1801 acactgagac aaaaaactct cactaagaca ttccactgtg gccctgctgc ctgatggggg
     1861 ccaggcaacc cctggaagag ttctactgcc ctctccatag ctactcttgg agccagaggc
     1921 ttgatcttca tggggcctta tgtggacacc accccaggtg aagtggaaag cccttccttc
     1981 cctgcctgga acagaagatg gagcagggag cttctaccca catctaacct ctgcatctgt
     2041 gggtactcag gagagtaagt gtggaaagga ccatgcgtgg cgcccattcc taacgtctac
     2101 caagttatac actggaatac aggagagctg cggcccaggc tgcctcgtcc taaattctct
     2161 ttcgggggat cctgtaaagg cagggcagct ttaggaagta ccttcttggt gcacacacca
     2221 aagtgcttat ggaaactggt ctcccctacc tggccgttgc tgtcactgtc cacacagaac
     2281 tgtacactgg taacggggac atgcctttgt catgagaggc tcagaactgg tggtcctgtc
     2341 ggactgtctt cccatttgct ggcctctacc tgtaggactg aggaaggaaa ggacaaggca
     2401 tgaggcagcc cagaccccag gaggagtcgc tggccccact ctagttttga ttcaagtaca
     2461 aattgagtcc tagtgcaaac agccttatga agcctggtgc ctcccatgcc ccgagttctt
     2521 gtgggtccac atagcagagt caggctccat gtttcagctg accagcaagg tgataggcag
     2581 gttgtggagc agggcaagaa actttctaca gcagtaactg gttgctctag tgtccacaga
     2641 gcagccatgg ggaagccatg ccctgcatcc aagaaccgtt caaagcccaa atctggcaga
     2701 atagtccacc ttttccagcc ctgccagtcc tgggctgtgt agcttgcagc tgccatctgc
     2761 ccctgagaag tcagtgccat tattccaaac tgctgtctgt gcgggcctag ggcgggactc
     2821 ctgggctcat ctcctcagac ctccatgtcc tagctcggag ctggaagacc ataccactgc
     2881 cttcgggaca tgggccctgc agcttctgcc ccgtgtccct cagagtctga gtgacgttca
     2941 tcatatcatc tggacacttg ttagccatag cagatgcctg tggtcggcca ctgtggataa
     3001 tggctggatg cagcttgagg caaagaccac caaagccttt ttcctctgtg ccccgggaaa
     3061 gcaggaactg atggacccag aggtgtaaga gcccagggtg ggggcagagg gatgtcagtg
     3121 gagcaggagt tgtgctggaa cagcccctcc ctccagcccc tgaagtggct tccgcaaggc
     3181 agtcgcatgg ggggctggtt gtcaggcttt agctgagttg gttcaggggt tcacaggcgg
     3241 actgcctaca tttcctgctt tccctagaat gtattatcct ggttttcact tgtgcattgc
     3301 actgtgtcca gcattctttg caataaatac tttaaaaaac aaaacaaaca aacaaaaaca
     3361 aaaaaaaaaa aaaaaaa
//
