LOCUS       NM_001177756            5153 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
            (Pfkfb3), transcript variant 5, mRNA.
ACCESSION   NM_001177756
VERSION     NM_001177756.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Kwon,H.J., Hahn,K.R., Moon,S.M., Yoo,D.Y., Kim,D.W. and Hwang,I.K.
  TITLE     PFKFB3 ameliorates ischemia-induced neuronal damage by reducing
            reactive oxygen species and inhibiting nuclear translocation of
            Cdk5
  JOURNAL   Sci Rep 14 (1), 24694 (2024)
   PUBMED   39433564
  REMARK    GeneRIF: PFKFB3 ameliorates ischemia-induced neuronal damage by
            reducing reactive oxygen species and inhibiting nuclear
            translocation of Cdk5.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Gallerand,A., Dolfi,B., Stunault,M.I., Caillot,Z., Castiglione,A.,
            Strazzulla,A., Chen,C., Heo,G.S., Luehmann,H., Batoul,F.,
            Vaillant,N., Dumont,A., Pilot,T., Merlin,J., Zair,F.N.,
            Gilleron,J., Bertola,A., Carmeliet,P., Williams,J.W.,
            Arguello,R.J., Masson,D., Dombrowicz,D., Yvan-Charvet,L., Doyen,D.,
            Haschemi,A., Liu,Y., Guinamard,R.R. and Ivanov,S.
  TITLE     Glucose metabolism controls monocyte homeostasis and migration but
            has no impact on atherosclerosis development in mice
  JOURNAL   Nat Commun 15 (1), 9027 (2024)
   PUBMED   39424804
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5153)
  AUTHORS   Wang,X., Liu,X., Wu,W., Liao,L., Zhou,M., Wang,X., Tan,Z.,
            Zhang,G., Bai,Y., Li,X. and Zhao,M.
  TITLE     Hypoxia activates macrophage-NLRP3 inflammasome promoting
            atherosclerosis via PFKFB3-driven glycolysis
  JOURNAL   FASEB J 38 (15), e23854 (2024)
   PUBMED   39096131
  REMARK    GeneRIF: Hypoxia activates macrophage-NLRP3 inflammasome promoting
            atherosclerosis via PFKFB3-driven glycolysis.
REFERENCE   4  (bases 1 to 5153)
  AUTHORS   Imbert-Fernandez,Y., Chang,S.M., Lanceta,L., Sanders,N.M.,
            Chesney,J., Clem,B.F. and Telang,S.
  TITLE     Genomic Deletion of PFKFB3 Decreases In Vivo Tumorigenesis
  JOURNAL   Cancers (Basel) 16 (13), 2330 (2024)
   PUBMED   39001392
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5153)
  AUTHORS   Guzel,S., Yalcin,A., Gurpinar,Y. and Guler,S.
  TITLE     Expression of Pfkfb isoenzymes during in vitro differentiation of
            mouse embryonic stem cells into insulin-producing cells
  JOURNAL   Turk J Med Sci 53 (6), 1565-1573 (2023)
   PUBMED   38813509
  REMARK    GeneRIF: Expression of Pfkfb isoenzymes during in vitro
            differentiation of mouse embryonic stem cells into
            insulin-producing cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5153)
  AUTHORS   Duran,J., Navarro-Sabate,A., Pujol,A., Perales,J.C., Manzano,A.,
            Obach,M., Gomez,M. and Bartrons,R.
  TITLE     Overexpression of ubiquitous 6-phosphofructo-2-kinase in the liver
            of transgenic mice results in weight gain
  JOURNAL   Biochem Biophys Res Commun 365 (2), 291-297 (2008)
   PUBMED   17991424
REFERENCE   7  (bases 1 to 5153)
  AUTHORS   Mykhalchenko,V.G., Minchenko,D.O., Tsuchihara,K., Moenner,M.,
            Komisarenko,S.V., Bikfalvi,A., Esumi,H. and Minchenko,O.H.
  TITLE     Expression of mouse
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 mRNA
            alternative splice variants in hypoxia
  JOURNAL   Ukr Biokhim Zh (1999) 80 (1), 19-25 (2008)
   PUBMED   18710022
  REMARK    GeneRIF: Possible role of PFKFB-3 splice isoform in cell adaptation
            to hypoxic conditions.
REFERENCE   8  (bases 1 to 5153)
  AUTHORS   Chesney,J., Telang,S., Yalcin,A., Clem,A., Wallis,N. and Bucala,R.
  TITLE     Targeted disruption of inducible 6-phosphofructo-2-kinase results
            in embryonic lethality
  JOURNAL   Biochem Biophys Res Commun 331 (1), 139-146 (2005)
   PUBMED   15845370
  REMARK    GeneRIF: Inducible 6-phosphofructo-2-kinase expression is essential
            for the survival of the growing embryo.
REFERENCE   9  (bases 1 to 5153)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   10 (bases 1 to 5153)
  AUTHORS   Minchenko,O., Opentanova,I. and Caro,J.
  TITLE     Hypoxic regulation of the
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene family
            (PFKFB-1-4) expression in vivo
  JOURNAL   FEBS Lett 554 (3), 264-270 (2003)
   PUBMED   14623077
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY267778.1, BC052400.1, EU034679.1, BB553577.2 and BP764506.1.
            
            Transcript Variant: This variant (5) lacks one alternate exon in
            the 3' coding region, compared to variant 2. The resulting isoform
            (5) has a shorter and distinct C-terminus, compared to isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU034679.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                BY267778.1         1-23
            24-390              BC052400.1         1-367
            391-2152            EU034679.1         1-1762
            2153-4735           BC052400.1         2096-4678
            4736-5124           BB553577.2         274-662
            5125-5153           BP764506.1         26-54               c
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 8.73 cM"
     gene            1..5153
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     3"
                     /db_xref="GeneID:170768"
                     /db_xref="MGI:MGI:2181202"
     exon            1..466
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            467..592
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            593..689
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            690..756
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            757..831
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            832..888
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1013
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1014..1221
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1368
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1473
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1603
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1666
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1731
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..1818
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1992
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2015
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2016..5129
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4712..4717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /note="hexamer: AATAAA"
     polyA_site      4734
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /note="major polyA site"
     polyA_site      4769
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
     regulatory      5108..5113
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
                     /note="hexamer: AATAAA"
     polyA_site      5129
                     /gene="Pfkfb3"
                     /gene_synonym="E330010H22Rik; iPFK-2; uPFK-2"
ORIGIN      
        1 gccgcgctac cggtcagatc ttaaaaggcc gggcagtgcg tgtggcatcc cgacagaact
       61 ggagaggagc aagtggcagt gctgggtgtt gggaccgtgg ctgtcactgc gctggagcat
      121 ccctgagctt ttgaacagag ccgactagtc agcagtgcag cagccgcaac tccacgccgc
      181 tctcatctgt ctcgtgtctc cgctcaggcg atccttcctc cgagactccg cacaaagcac
      241 attttccaaa gcagtgcaag ggagcgccca gccgcgtgca ggcggacgca cccttcctgt
      301 cctttgttcc ggggggatct gctgctgctc ttctccccaa gtcggagtct ttcctccagg
      361 ggtggcgggt cgccgaatac agctacgaag atgccgttgg aactgaccca gagccgggta
      421 cagaagatct gggtgcccgt cgatcaccga ccctcgttgc ccagatcctg tgggccgaag
      481 ctgaccaact ccccaaccgt gattgtgatg gtggggctcc cagcccgggg taagacttac
      541 atctccaaga agctgactcg ctacctcaac tggataggtg ttccaacgaa agtgttcaat
      601 gtgggagagt atcggcgtga ggctgtgaag cagtacagct cttacaactt cttccgccct
      661 gacaatgagg aagccatgag agtccgaaaa cagtgtgcct tagctgccct gagagatgtc
      721 aaaagctacc tgacaaagga aggtggacag attgcagttt ttgatgccac caatacaact
      781 agagaaagaa ggcacatgat ccttaatttt gccaaagaaa atgacttcaa ggcatttttc
      841 atcgagtcgg tctgtgacga ccctactgtt gtggcctcca acatcatgga agttaaaatc
      901 tccagcccgg attacaaaga ctgcaactca gcagaagcca tggacgactt catgaagaga
      961 atcaattgct atgaagccag ctaccagcct cttgaccctg ataaatgtga cagggacttg
     1021 tcactcatca aagtgattga tgttggccgg aggttcttgg tgaacagagt gcaggaccac
     1081 atccagagcc gaattgtata ctacctgatg aacatccacg tgcagccccg taccatctac
     1141 ctgtgtcggc atggcgagaa tgagtacaac ttgcagggca agattggggg cgactcaggc
     1201 ttgtccagca gaggcaagaa gttcgccaat gccctgagca agttcgtaga agaacagaat
     1261 ctgaaggacc tccgtgtgtg gaccagccag ctgaaaagta ccattcagac cgcggaggcc
     1321 ctgaggctcc cctacgagca gtggaaggca ctcaacgaga tcgatgctgg tgtgtgtgag
     1381 gaactaacct atgaggagat cagagatacc taccctgagg agtacgcact gcgtgaacag
     1441 gacaagtact actatcgcta tcccacggga gagtcctacc aggatcttgt ccaacgcctg
     1501 gagcctgtga tcatggaatt agagcgccaa gagaatgtat tggtcatctg tcaccaggct
     1561 gttctacgct gcctactagc ctacttcctc gacaagagtg cagaggagat gccatacctg
     1621 aaatgtccgc tccacactgt cctgaaactg acacctgtgg catatgggtg ccgggtggag
     1681 tccatttact tgaatgtaga atcggtgagc acacaccggg agaggtcaga ggctgtcaaa
     1741 atacaacact ttgccagtgt agtcagacct tcctcttaca ccgaacttga ctttcagtct
     1801 gtggagtccg caaaacagga tgcaaagaag ggacctaacc cgctcatgag acgcaatagt
     1861 gtcaccccac tagccagtcc cgagcccacc aaaaagcctc gcatcaacag ctttgaggag
     1921 cgtgtggctt ccacttctgc tgccctgcca agctgcctgc ccccggaagt gcccacgcag
     1981 ctgcccggac aacctttgct agggaaagcc tgtctaacat gaagagctcc cggagcggtg
     2041 ccgactcttc ccagaagcac taaagccgag atgtgggttc cattccattt ccacagcctg
     2101 ccttgtgcct gctcatcttc tcgaggccaa gtgatcgcaa ggacttctgg actcgggaga
     2161 atggaggtcg gcatgttgaa gagtagaagc ccttcccccc actgaaatgt tggggtggca
     2221 aagacaacta ggaagttgta cagatcccag cttcctgtgt agagcagcag accttcctac
     2281 gctctgttct ctttcgactt ccagggctgt ctgacctctc aggcccttcc aaagcttggg
     2341 gagggctata gttctagagg aggtgagatc agacccctaa gtgggggccc tggacaccgc
     2401 cacgctgttt tgttttgttt ctttggatcc tggcctggca tgtcgatagt cagaagataa
     2461 gagtggcagg gaaccctgag attcagagtt agaccccact tgccagaggc tcagggacaa
     2521 gttgcacatt ctcatgtggc ttttatgcat cttggcctag gaggaggcct cagagacaag
     2581 aggcttgggc attgctgcat tgggtatggg atttctcctc ctgaggaaac cttgagctga
     2641 ggcctactgg tcagctgagc aatgacttgc cctgactcct ctacaggtcc acatgtcttc
     2701 tgggtcccaa gttaagggac agactggttg tatcagtgga aggaaggatt tttcagaaaa
     2761 aaataaccag ggctcccctc ttcccagagt cccttaggcc ttctgttctt caccttgggc
     2821 ctggaggcac ctgagtagat tctgttgttg gctgtttgtt tgcacttttc cttctttctt
     2881 tccttccttc cttccttcct tccttccttc cttccttcct tcctccctcc ttccttctct
     2941 tcttctctcc ttccttccct ccctccccac acctcacttc aagaacctag ggtgctggcc
     3001 cttggaggct tgcagcttct gaatgggagg agttggggtg tctttaagat ggggagtcta
     3061 agagccttca acttcactcc cattgttcaa gaagggacgg tggcccaagg aaggctcatg
     3121 tcaggccgcc tgctcagagc tgtccgcata ttttggccaa gtgtctgctg ctgtatgcct
     3181 cataccacca tgctggccat cttgaggagt cacccccagt gtagaaaagc atccttgtcg
     3241 gctttatggc attggcgaca tttttgcatg ggttgaaaag tagcgctaat gtctttgtct
     3301 tcctgtttct cccctttgtc cacccatgtt ctgggggagg acgggacatc ccaagcctgg
     3361 aagcaaccaa tgggaaactc agcagtgtca gcccaaagga ccctttatga cgatgctgca
     3421 caactagggc cgaaggtcac tctttacttt ctgcttcctt cctgggagtc ctagggatcc
     3481 tctcttcccc agctcagggg acaacagatc ctggggtgac acagggcatg cttcagacac
     3541 tttaaagtca ccttgtgttt tcatagctaa ctactcaaac atttggccca gcccactacc
     3601 ctttatatct gtggttcacc aggatataga tagatgatag atagaaagat agatagatag
     3661 atagatagat agatagatag atagatagat agatagatga tagatatctc catctgaaga
     3721 cttggacttt tttctctatg gcttgagtct tgacaaggat atatgaactg cagacgtggg
     3781 ctgacatgtg tgcaggctga taatattttt ggacctgaag atctgcagcg ttgatttggg
     3841 ggatagggtt gggggtgggg tagggaacag gataagaact gtgtagccca aactagcctc
     3901 aaactcatgg ccgtcctcct gtctcagcct ctcaaatgtt agctgttcgg gttataagca
     3961 tgagccacca ccacaccacg cctggtacta tggctttgta cctaaatgtg gtttcttcga
     4021 tgcttcctcc tgaccatatc cattttatgg ttatagaacc agcttttaca acatagagac
     4081 tctcaaggct gagacaggta ggggtggtca tgtgtgacca gcctaagtca atacatcaag
     4141 agaaagcttt gcccaatgtg atttcacatt ctgtgaacac ctatgaataa gatcaggccc
     4201 aggagatctt gatgcgggtg gaacatgcat ccttcaaaag ccagtgactt ggtggatggg
     4261 gtgggtagca cccatagaac ttccactctc ccacccaaaa cagcaaaata aaatggggac
     4321 caatgttttt aacgttgcat atttgttttg ggggtaaatt tctccccttt ccttcaacaa
     4381 tgggcactac ccttgattta tggggagaag aaaaactgtt ggaaccacac caatgatatt
     4441 ttcatttgta atacttgaag tttatttttt tattattttg atagcagatg tgctatttat
     4501 ttatttaata tgtataaggg agcctaaaac tagaaagctg tctagattgg ctttaattgt
     4561 tcattggttt ggggcctttc aatgtgtgac tgatgacttc ttcatgttct gtgtcattgt
     4621 ctctgtccgt gacctaggaa gaagctttgc aagcttttta acaggacacg ccttcctttc
     4681 agacatctgt gtagtttgca gttgcaagaa caataaaaac ctggttgaga tacacctgcg
     4741 ggactcgctt ctcaggtttt tgcggagaac aagtgtcagt tttctttcta agcatttagt
     4801 gcgtggttgt gtgtgtgtgt gcgtgcgcat cagtgtgtgt gtgccagctc atacatgtgc
     4861 acgggggttc acacatgtag gggtcagagg acagcttgac aggagtccat ctctcccagc
     4921 ctccagagat tgagcccagg cctccaggct tgtcagtgcc tttacttgag tcaatcattt
     4981 tactggacca cagggtcttt tattgagatt tatggccagc gcatctctca gctccttgca
     5041 gaaccctgtt ttatcttcct ttaaataaga aacaacagat tgtcaagttt gtatatttag
     5101 gaaatataat aaaatgtgtt aattttaaaa aaaaaaaaaa aaaaaaaaaa aaa
//
