LOCUS       NM_008825               7308 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
            (Pfkfb2), transcript variant 1, mRNA.
ACCESSION   NM_008825
VERSION     NM_008825.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Harold,K.M., Matsuzaki,S., Pranay,A., Loveland,B.L.,
            Batushansky,A., Mendez Garcia,M.F., Eyster,C., Stavrakis,S.,
            Chiao,Y.A., Kinter,M. and Humphries,K.M.
  TITLE     Loss of Cardiac PFKFB2 Drives Metabolic, Functional, and
            Electrophysiological Remodeling in the Heart
  JOURNAL   J Am Heart Assoc 13 (7), e033676 (2024)
   PUBMED   38533937
  REMARK    GeneRIF: Loss of Cardiac PFKFB2 Drives Metabolic, Functional, and
            Electrophysiological Remodeling in the Heart.
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Li,M., Wu,X., Pan,Y., Song,M., Yang,X., Xu,J., Plikus,M.V., Lv,C.,
            Yu,L. and Yu,Z.
  TITLE     mTORC2-AKT signaling to PFKFB2 activates glycolysis that enhances
            stemness and tumorigenicity of intestinal epithelial cells
  JOURNAL   FASEB J 38 (5), e23532 (2024)
   PUBMED   38451470
  REMARK    GeneRIF: mTORC2-AKT signaling to PFKFB2 activates glycolysis that
            enhances stemness and tumorigenicity of intestinal epithelial
            cells.
REFERENCE   3  (bases 1 to 7308)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 7308)
  AUTHORS   Guzel,S., Yalcin,A., Gurpinar,Y. and Guler,S.
  TITLE     Expression of Pfkfb isoenzymes during in vitro differentiation of
            mouse embryonic stem cells into insulin-producing cells
  JOURNAL   Turk J Med Sci 53 (6), 1565-1573 (2023)
   PUBMED   38813509
  REMARK    GeneRIF: Expression of Pfkfb isoenzymes during in vitro
            differentiation of mouse embryonic stem cells into
            insulin-producing cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7308)
  AUTHORS   Fu,C., Yu,S., Liu,Z., Wang,J., Liu,P. and Su,G.
  TITLE     PFKFB2 Inhibits Ferroptosis in Myocardial Ischemia/Reperfusion
            Injury Through Adenosine Monophosphate-Activated Protein Kinase
            Activation
  JOURNAL   J Cardiovasc Pharmacol 82 (2), 128-137 (2023)
   PUBMED   37155368
  REMARK    GeneRIF: PFKFB2 Inhibits Ferroptosis in Myocardial
            Ischemia/Reperfusion Injury Through Adenosine
            Monophosphate-Activated Protein Kinase Activation.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7308)
  AUTHORS   Cheverud,J.M., Ehrich,T.H., Hrbek,T., Kenney,J.P., Pletscher,L.S.
            and Semenkovich,C.F.
  TITLE     Quantitative trait loci for obesity- and diabetes-related traits
            and their dietary responses to high-fat feeding in LGXSM
            recombinant inbred mouse strains
  JOURNAL   Diabetes 53 (12), 3328-3336 (2004)
   PUBMED   15561968
REFERENCE   7  (bases 1 to 7308)
  AUTHORS   Donthi,R.V., Ye,G., Wu,C., McClain,D.A., Lange,A.J. and
            Epstein,P.N.
  TITLE     Cardiac expression of kinase-deficient
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase inhibits
            glycolysis, promotes hypertrophy, impairs myocyte function, and
            reduces insulin sensitivity
  JOURNAL   J Biol Chem 279 (46), 48085-48090 (2004)
   PUBMED   15331593
REFERENCE   8  (bases 1 to 7308)
  AUTHORS   Minchenko,O., Opentanova,I. and Caro,J.
  TITLE     Hypoxic regulation of the
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene family
            (PFKFB-1-4) expression in vivo
  JOURNAL   FEBS Lett 554 (3), 264-270 (2003)
   PUBMED   14623077
REFERENCE   9  (bases 1 to 7308)
  AUTHORS   Shin,D.J. and McGrane,M.M.
  TITLE     Vitamin A regulates genes involved in hepatic gluconeogenesis in
            mice: phosphoenolpyruvate carboxykinase,
            fructose-1,6-bisphosphatase and
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
  JOURNAL   J Nutr 127 (7), 1274-1278 (1997)
   PUBMED   9202079
REFERENCE   10 (bases 1 to 7308)
  AUTHORS   Batra,R.S., Brown,R., Brown,G.K. and Craig,I.W.
  TITLE     Molecular cloning and tissue-specific expression of mouse kidney
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
  JOURNAL   FEBS Lett 393 (2-3), 167-173 (1996)
   PUBMED   8814283
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC139231.11.
            
            On Jun 23, 2009 this sequence version replaced NM_008825.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.815.1, BC018418.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               AC139231.11        132468-132568
            102-212             AC139231.11        133713-133823
            213-338             AC139231.11        140088-140213
            339-435             AC139231.11        140684-140780
            436-502             AC139231.11        141171-141237
            503-577             AC139231.11        141372-141446
            578-634             AC139231.11        141807-141863
            635-759             AC139231.11        142788-142912
            760-967             AC139231.11        145851-146058
            968-1114            AC139231.11        146438-146584
            1115-1219           AC139231.11        147415-147519
            1220-1349           AC139231.11        148179-148308
            1350-1412           AC139231.11        149039-149101
            1413-1477           AC139231.11        149546-149610
            1478-7308           AC139231.11        150204-156034
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 56.89 cM"
     gene            1..7308
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     2"
                     /db_xref="GeneID:18640"
                     /db_xref="MGI:MGI:107815"
     exon            1..101
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            102..212
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..1678
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="isoform 1 is encoded by transcript variant 1;
                     PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase 2;
                     6PF-2-K/Fru-2,6-P2ase heart-type isozyme; PFK-2/FBPase-2
                     gene B;
                     6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 2 isoform 1"
                     /protein_id="NP_032851.2"
                     /db_xref="CCDS:CCDS35701.1"
                     /db_xref="GeneID:18640"
                     /db_xref="MGI:MGI:107815"
                     /translation="MSENSTFSPEDCNSSYKPHASNLRRAGKTCSWASYMTNSPTLIV
                     MIGLPARGKTYVSKKLTRYLNWIGVPTKVFNLGVYRREAVKSYQSYDFFRHDNEEAMK
                     IRKQCALVALEDVKAYFTEESGQIAVFDATNTTRERRDMILNFAKQNAFKVFFVESVC
                     DDPDVIAANILEVKVSSPDYPERNRENVMEDFLKRIECYKVTYQPLDPDNYDKDLSFI
                     KVINVGQRFLVNRVQDYIQSKIVYYLMNIHVHPRTIYLCRHGESEFNLLGKIGGDSGL
                     SVRGKQFAHALKKFLEEQEIQDLKVWTSQLKRTIQTAESLGVTYEQWKILNEIDAGVC
                     EEMTYSEIEQRYPEEFALRDQEKYLYRYPGGESYQDLVQRLEPVIMELERQGNILVIS
                     HQAVMRCLLAYFLDKGADELPYLRCPLHIIFKLTPVAYGCKVETITLNVDAVDTHRDK
                     PTHNFPKSQTPVRMRRNSFTPLSSSNTIRRPRNYSVGSRPLKPLSPLRALDMQEGADQ
                     PKTQVSIPVV"
     exon            213..338
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            339..435
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            436..502
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            503..577
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            578..634
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            635..759
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            760..967
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1114
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1219
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1349
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1412
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1477
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1478..7308
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7281..7286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /note="hexamer: AATAAA"
     polyA_site      7308
                     /gene="Pfkfb2"
                     /gene_synonym="4930568D07Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggagcccgga ctacttcagt ctgtctgcca gctttggtac ccggtgctcc ggtggcgaca
       61 ctgggtctac agcagggagg acgaatagga caggtcatag ggtgcctcct gaagaactac
      121 catgtctgag aatagtacat tttccccaga agactgcaac agcagctata aaccccacgc
      181 ctcaaatctg cgaagggcag ggaagacatg ctcatgggct tcctatatga ccaactcccc
      241 aacactcatt gttatgattg gcttgccagc ccggggtaaa acttatgtgt ctaagaaact
      301 aacacgctac ctcaactgga ttggagtacc caccaaagtg tttaatcttg gggtatatcg
      361 acgtgaagca gtcaagtcct atcagtctta tgatttcttt cgacatgaca atgaagaggc
      421 tatgaagatc cgcaaacagt gtgctctggt ggcactggaa gatgttaaag cctattttac
      481 tgaagagagt gggcagattg cggtgtttga tgccaccaat accactcggg agaggaggga
      541 catgattttg aactttgcca agcagaatgc cttcaaggta ttttttgtag aatctgtctg
      601 tgatgatcct gatgtcattg ctgccaatat tctggaggta aaagtgtcga gccctgacta
      661 ccccgaaagg aatagggaga atgtgatgga ggacttcctg aagagaattg agtgctacaa
      721 ggtcacttac cagccccttg acccagacaa ctatgataag gatctttctt tcataaaggt
      781 gataaatgta ggccagagat tcctggtcaa cagagttcag gactacatcc agagtaagat
      841 tgtctactac ctgatgaata tccacgtcca tcctcgcacc atctaccttt gccggcacgg
      901 cgagagcgag ttcaaccttt tggggaagat tgggggtgac tctggcctct ccgtgcgagg
      961 aaagcagttt gctcatgctc tgaagaagtt tctggaggaa caggagatcc aggaccttaa
     1021 agtgtggacg agccagttga agaggacaat tcagactgct gagtctcttg gggtgaccta
     1081 tgagcagtgg aagatcctca atgagattga tgctggcgtg tgtgaggaga tgacatattc
     1141 agagattgag caacggtacc cagaggaatt tgcactccga gatcaagaga agtatctgta
     1201 ccgataccct ggtggggagt cctaccagga cctggtgcag cggctggagc ctgtcatcat
     1261 ggagctagag cgtcaaggca acatcctcgt tatctctcac caggctgtca tgcgctgcct
     1321 cctggcctac ttcttggata aaggcgcaga tgagttacca tacttgaggt gccccctgca
     1381 catcatcttc aaacttactc ctgtggccta tggttgcaaa gtggaaacaa ttactctgaa
     1441 tgtggatgct gtagacacac atcgtgacaa gccaactcac aacttcccca agagccaaac
     1501 ccctgtaagg atgagaagga acagctttac gcctctgtcc agttcgaaca caataaggcg
     1561 tccaagaaat tacagtgttg ggagccgacc cctcaagccc ctcagccctc tccgtgctct
     1621 ggacatgcaa gaaggggcgg accagccgaa gacccaagtc agcattccgg tggtgtaact
     1681 gtgtgtttcc ctccagcttt ggcctcttgc ccttgccact aatcaaccaa gaagtcattt
     1741 aacttccccc cactcctgtc ccaacgttaa tctgaaaaca gaatctgagc tcatgcgtgt
     1801 gcagagcagc ctcagccatt ctctagtgta aacacgtcac ccgggggcaa gatggcaagt
     1861 tgggtcttta ggacatgtcc ccagatggga tgatctgaaa gaagaggagc caagcatgag
     1921 tcatagactt ggttcttctg tttcctgatg tctgacccag tctgtgaagt ctgtgtgtgt
     1981 gtatgtgtgt gtatgtatgc atgtgtgtgt atgtatgtgt gtgtgtgtat gtatgtatgt
     2041 gtgtgtatgt atgtatgtat gtgtatgtat gtatgtgtgt gtatgtatgt atgtatgtgt
     2101 gtgtatgtat gtatgtatgt gtgtgtatgt gtatgtatgt gtgtgtatgt atgtatgtat
     2161 gtatgtgtgt gtatgtatgt atgtgtgtgt ttgtgtatgt gtgtggaagg ggggttgttg
     2221 ggaggggatg tgggatagtg tgagatgtaa caacctaatt gtccttcaca tgtgtccttt
     2281 aagtctatcc ttatatcctt aacttccctg tcctatagct tctctttggg ttgtctggtg
     2341 tgatgcatgg cagtatgatg tcttaaagta agggactgtt gataagtatc taagccccat
     2401 tggtgctgtg tgcactcggg aatggatcta cactgggaga gtcttttccc ttcccttctc
     2461 ccttcctctc cagccaggtc cagcaagggt cagcccaggc tacagaacat ccacagaaag
     2521 ccctggagtt tgcaaatgga catagggtga gagctcttgc ctcttttcat gagaaacaat
     2581 cctaagtttt tgctgccctg gagagctggc tctaagaatg tggatatgtc ttggtttgcg
     2641 ttccgaactt gaggagcccg acagtccttt gccggctgtg aaatggggct gggccagggt
     2701 aaagtctagt tcttagaaat gtacagttca gaatttttgt cctatggctc tcattttctt
     2761 tgccattcca gtaactttcc tgtagcccgt gacctttggg gctgtttaaa agcaccattg
     2821 agccctgagt ttctccaggc aaaagtgaag gtctccaata cgttcagtaa agctgctcat
     2881 tgtttaccag tactttttgg aataatttcc taaagctaag tttttacatg aaaagttatt
     2941 ctttggttgt gaaaaaagtt ttgatgatga atttataacc tgacttattg ggggcatttt
     3001 gtttatgtaa gacaatatca caatattcta agaagtcttc tgggatttgg ctcttgaaag
     3061 tttatttgct taaaggggat tctccatatt gtcccagggc ccagttttgc taagggagca
     3121 ccctgctgcc gagctcaatg ccaggccact ctggaacatg attctaaggc acaaatctaa
     3181 aggtctcgtg agatttttca gaaaagacac atgactccct acctctcagt ttgtcatgtg
     3241 gggccaggtg gtgagagaaa gttagcaatt ttctttgttt ttgtcttgta atagaaagct
     3301 ttttgcatgg ttcttatcca gaaacactcc ttatactgtg ccatagaaat tttgaaatac
     3361 atacatacat acatacacac acacacacac acacacacac acacacacac acaccttggt
     3421 tcccttgaga cagactgagt gctgcctcca aactctctgg gattgggttc ttgatatttg
     3481 ggtgtctaga taacttccta aagagttagt cccaggttta gaatgccact cgaggagctg
     3541 gagagatggc tcagttacaa cagctcagtt aagcttccag aggtccaaag cttagtccct
     3601 agcattccta ttgagtggct tatagcagcc tgtaactcca gctctgtggt atctgatgcc
     3661 ttcttctggg tgccccaggt actttcacat atgtagctca tatacacaca ggcacacata
     3721 tacacataaa taatactaaa actaaatctt ttttttcagt cccactttat gaagctgaat
     3781 agaaaaattc cagtctatta gtatcttaga caaagtacca gactggaaat tatgaattct
     3841 tggttctgaa aagacagttc tttggaatta gatgcttagg cagaacagtt ttgacagaag
     3901 tcaccccttc aaaggcaggc tgctttgtta gaaaggtatt ctagattcta gtcccatctt
     3961 ttatccttgg cactgcaagg gggctatgca acaaattatt gatttaaaca catgttcttg
     4021 gtgcacactg aaaatgtact caaggtgaaa tgcactgtga tggaatgcat ggcctgatgg
     4081 gggttctcat cacaggctgc tgacaactgg tccatataga tgctacctac cttcctgaaa
     4141 ctcatccaag acaaagcaac tgggaatcca gtcaggcagt gtgtactgtc tcctgtttgt
     4201 ctttgatggt gggctctggg tgtagtgtgg aaaactatga cagaacaaaa atagtcagtt
     4261 ttctggacct ggtgactgca aacaagcaaa acaggagaac agcaacaaat gacctttgag
     4321 cagtcagacg tgtacctgtg ttcaaggctg agattagata acctatctaa aggcttgttc
     4381 ctggagaaga ctaaatcttc ctctctcagg agccattgag tacatgtggc tcttcatcta
     4441 gagtgagtat gtatgtgtgt atatatatat atacacacat atacatatac acacatacac
     4501 atacatacat atatatatat acacatacat acatacatat atatatatat atatatacac
     4561 acatgtacaa gccacaataa ctagaaggga ttatgatatt gagggagtgg gagaaacctt
     4621 ggaagagttg gatggggagc aggaggccta gaatacagtg tgtgtgtgtg tgtgtgtgtg
     4681 tgtgtgtgtg tgtgtgtgtg tgtgtgtgaa atattaaaaa taaaaaagac aggagatgca
     4741 attgatagat gaatatttga aaaacaaaca tcctagaaga gctaacatta gagtagagta
     4801 taacttgtcc tctgactgtt tccttgttct gtggctgtga gacccaaggg ggtggcatgt
     4861 cctctcatca cagtgtgagg atgagatatg gcctgcttgg tgtgtctggc acttttttca
     4921 tagttgcctc gtctactgct tagttattgc aactctggtc caaattggaa ttcagtcttt
     4981 cttctgctgc aaggcaagag tagtccagag ctactgagtg ggaagagaaa gaacattgat
     5041 ctaagagcta ctaaggatct agcttgtaag aggagacttt tctgaccact tggaattgag
     5101 ctagcctgtt gaacgtgcca tctttaggag tggactctca gtctactgct tgctcttctc
     5161 attcccactg tgttagttca cttctatagg aagtcttgtt atccctgcat aacaaggaac
     5221 aaaagttgga gctgaaaagc agcaggagtc tcaacatcct tttagaacaa ggaccttccg
     5281 agtagctgag caggagcagt agggagacac gagtagaaag tacttattcc agagtttagg
     5341 gaagtttacc aggctgtgct tgtgtctatc acaaacccca caaagagaat ataacctcta
     5401 gagtacctgg agttgagact ttcagtttcc tcatggggaa aataggtgaa gacttgtata
     5461 aaacagggaa gagaaaataa tagtttgctt atttatttat atttatttat tttttaacac
     5521 acaactgaat ggaaaataaa accctgaaaa atggtgggag gggtcctgga gatatagttc
     5581 agtggttgag tacttgccta gcctatagga ggccctgggt ttcatcccca gtactgggga
     5641 aaacaaaaac aaaacacaaa acaacactga atgagaaagt cagggaatgg acttgaaaga
     5701 gcaactgaaa ggagatttag agaaccagta ctgtcctgcc ttgtttccaa actagaattt
     5761 agcattttct gatcattggg ctagggcttt ttctcttggt aaatggatct tttaatcatt
     5821 cttagacatg cattatgctg cgtgcttgcc attatttctg gttcctaagg caacattagc
     5881 gatacaacat acttctgttc aaggccagcc tggttctaca gagtgagttc caggacagcc
     5941 agggctacac agagacacct tgtcttgaaa aacaaaacaa aaaaacaaac aaacaaacaa
     6001 aaacccccca aaatccaaac agtacagttg ttagggaata aatctgcctg atgattcttg
     6061 tggggacagg tgtgatctaa gtcccagagg atctcattag tgcttggggt ggcgggtgac
     6121 aggagagacg tgccaggtag ttgaggcagc tctgagtgat gaggtttcat gcaccttcca
     6181 ctgccctggc atctcagaag aatgttgtgt ttgctgacaa cagtttcagt aaattaccca
     6241 gctctccttc cttgatagac ttgtgtttta aggcattttt attttaaaag tgcctaacag
     6301 ttaagcagga cggctatgct agatcagagg tgatggacac acatcagaag tatgcctaag
     6361 aatttagttt tgtagtccag aagactaatg gtctttttgc caacatagtc catcctttct
     6421 cattgtgctg actggtagga tatgcacatt attatatctc cttgcctagt attcctatca
     6481 gaaacccaac attttttttt atatttcctt tatggaaaag ttctgtacac catggctttt
     6541 cacagacaat attgtaaaat agtggctcaa cgtctcctta tttttcaaaa gatgatgctg
     6601 tagattactt gtgtggcatt ttgtgatctg agctaaggta atgccagaag agttgtgact
     6661 ttacttgaag tcatttcatc cagtctcctg cctcagggta gaacttttct tttactatct
     6721 gttcctgaac cttctgtatc cttacccttc cctgttgtgg gctgtagtat gctcactgag
     6781 agggctgctg tttttcatct gctgtgtgtt tagcctttac tcttaatctt atacaaaggg
     6841 aaatatgacc ctaagagtga gtcagagtat tccttcccat gagcctttta cagggtatgt
     6901 aactaatacg catatgactt ggttcaaaaa cattcagaat tgtgtgtagt tttttgttgt
     6961 tgaagcaaca aagtcaactt actcagatgt ggattttctg tggcaaatgc aaagggctta
     7021 cttattacaa gtggcgtgtg atgtagagag gtgccgagca ttccttgttt ggatggaaat
     7081 gggcagaatt gggaagccca gtaacagttt tattgcagat ttcttagtca cagctctgct
     7141 gaaagtgaag atgttaatcc ttagtagcat tctgatttat tcctgtgata ttgataatga
     7201 aatattgcaa caggtccatt taaagtgtat ataattgtat ttgaactgtg ttttatgtgc
     7261 atgtctgtat agaatgggtt aataaaattg ttgagtaact tgaactta
//
