LOCUS       NM_001374173            1922 bp    mRNA    linear   ROD 08-FEB-2025
DEFINITION  Mus musculus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
            (Pfkfb1), transcript variant 1, mRNA.
ACCESSION   NM_001374173 XM_006528755
VERSION     NM_001374173.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 1922)
  AUTHORS   Sakakibara,I., Wurmser,M., Dos Santos,M., Santolini,M.,
            Ducommun,S., Davaze,R., Guernec,A., Sakamoto,K. and Maire,P.
  TITLE     Six1 homeoprotein drives myofiber type IIA specialization in soleus
            muscle
  JOURNAL   Skelet Muscle 6 (1), 30 (2016)
   PUBMED   27597886
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1922)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 1922)
  AUTHORS   Leite,T.C., Coelho,R.G., Da Silva,D., Coelho,W.S.,
            Marinho-Carvalho,M.M. and Sola-Penna,M.
  TITLE     Lactate downregulates the glycolytic enzymes hexokinase and
            phosphofructokinase in diverse tissues from mice
  JOURNAL   FEBS Lett 585 (1), 92-98 (2011)
   PUBMED   21074528
REFERENCE   6  (bases 1 to 1922)
  AUTHORS   Shin,D.J. and McGrane,M.M.
  TITLE     Vitamin A regulates genes involved in hepatic gluconeogenesis in
            mice: phosphoenolpyruvate carboxykinase,
            fructose-1,6-bisphosphatase and
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
  JOURNAL   J Nutr 127 (7), 1274-1278 (1997)
   PUBMED   9202079
REFERENCE   7  (bases 1 to 1922)
  AUTHORS   Batra,R.S., Brown,R., Brown,G.K. and Craig,I.W.
  TITLE     Molecular cloning and tissue-specific expression of mouse kidney
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
  JOURNAL   FEBS Lett 393 (2-3), 167-173 (1996)
   PUBMED   8814283
REFERENCE   8  (bases 1 to 1922)
  AUTHORS   Kurland,I.J., el-Maghrabi,M.R., Correia,J.J. and Pilkis,S.J.
  TITLE     Rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.
            Properties of phospho- and dephospho- forms and of two mutants in
            which Ser32 has been changed by site-directed mutagenesis
  JOURNAL   J Biol Chem 267 (7), 4416-4423 (1992)
   PUBMED   1339450
REFERENCE   9  (bases 1 to 1922)
  AUTHORS   Darville,M.I., Crepin,K.M., Vandekerckhove,J., Van Damme,J.,
            Octave,J.N., Rider,M.H., Marchand,M.J., Hue,L. and Rousseau,G.G.
  TITLE     Complete nucleotide sequence coding for rat liver
            6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a
            cDNA clone
  JOURNAL   FEBS Lett 224 (2), 317-321 (1987)
   PUBMED   2856848
REFERENCE   10 (bases 1 to 1922)
  AUTHORS   Van Schaftingen,E. and Hers,H.G.
  TITLE     Inhibition of fructose-1,6-bisphosphatase by fructose
            2,6-biphosphate
  JOURNAL   Proc Natl Acad Sci U S A 78 (5), 2861-2863 (1981)
   PUBMED   6265919
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672150.19.
            
            On Sep 12, 2019 this sequence version replaced XM_006528755.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189679.70403.1,
                                           SRR5189682.92026.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-347               AL672150.19        44965-45311
            348-473             AL672150.19        59123-59248
            474-567             AL672150.19        61543-61636
            568-634             AL672150.19        63450-63516
            635-709             AL672150.19        64414-64488
            710-766             AL672150.19        71306-71362
            767-888             AL672150.19        72619-72740
            889-1096            AL672150.19        75694-75901
            1097-1243           AL672150.19        79277-79423
            1244-1348           AL672150.19        85060-85164
            1349-1478           AL672150.19        90118-90247
            1479-1541           AL672150.19        92452-92514
            1542-1606           AL672150.19        93706-93770
            1607-1922           AL672150.19        94100-94415
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 68.46 cM"
     gene            1..1922
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /note="6-phosphofructo-2-kinase/fructose-2,6-biphosphatase
                     1"
                     /db_xref="GeneID:18639"
                     /db_xref="MGI:MGI:107816"
     exon            1..347
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     CDS             251..1666
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /EC_number="2.7.1.105"
                     /EC_number="3.1.3.46"
                     /note="isoform 1 is encoded by transcript variant 1;
                     6PF-2-K/Fru-2,6-P2ase liver isozyme; PFK-2/FBPase-2 gene
                     A; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1"
                     /codon_start=1
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase 1 isoform 1"
                     /protein_id="NP_001361102.1"
                     /db_xref="CCDS:CCDS90763.1"
                     /db_xref="GeneID:18639"
                     /db_xref="MGI:MGI:107816"
                     /translation="MSREMGELTQTRLQKIWIPHSSSSSLLQRRRGSSIPQFTNSPTM
                     VIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEFFRPDNMEAQ
                     LIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESI
                     CNDPDIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYI
                     KIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGL
                     SARGKQYAYALANFIRSQSISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVC
                     EEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVIC
                     HQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDK
                     PENVDITREPEEALDTVPAHY"
     exon            348..473
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            474..567
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            568..634
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            635..709
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            710..766
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            767..888
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            889..1096
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1243
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1348
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1478
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1541
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1606
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1922
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1881..1886
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /note="hexamer: AATAAA"
     polyA_site      1922
                     /gene="Pfkfb1"
                     /gene_synonym="PFK/FBPase 1"
                     /note="major polyA site"
ORIGIN      
        1 agaggcctct gctgacttct gctcagcctc tggctctcgg cacccacatc cccacttgta
       61 catccgggag cagaagaaaa agacagagct ggagataacc tgtgagaggc tcagaagccc
      121 agtgcatccc cctgtcatca cccctgccta cctgctgctg tggccacggc aacagacaag
      181 acagctagga acataggaag tgaggtccaa taccttgtgg gcagtggtgt cattagctgc
      241 aacgcctaag atgtctcgag agatgggaga actcactcaa accaggttgc agaagatctg
      301 gattccacat agcagcagta gcagcctgct gcagcggcga aggggctcct ccataccaca
      361 gttcactaat tcccccacaa tggtgatcat ggtgggttta ccagctcgag gcaagaccta
      421 catctctacg aagctcacac gctatctcaa ctggatagga acaccgacta aagtgtttaa
      481 tttaggtcag tatcgacgag aggcagtgag ctacaggaac tatgaattct ttcgcccaga
      541 caacatggag gcccaactta tcaggaagca gtgtgctcta gcagccctaa aggatgtcca
      601 taagtatctc agccgtgagg aaggtcacgt tgcggttttt gatgccacca acactaccag
      661 agaacgacga tcattgattc tgcagtttgc taaagaacat ggttacaagg tcttctttat
      721 tgagtctatt tgtaatgacc cagacatcat tgcagaaaac atcaagcaag tgaaactcgg
      781 cagtcctgat tacatagact gtgaccaaga aaaggtttta gaagactttc taaagagaat
      841 tgagtgctat gagatcaact accagccttt ggatgaggaa ttggacagcc acctgtccta
      901 catcaagatc ttcgatgtgg gcacacgcta catggtaaat cgagtgcaag accacgttca
      961 aagccgtaca gcctactacc tcatgaacat ccatgtcaca cctcgatcta tctacctatg
     1021 ccgacatggt gagagtgaac tcaaccttag aggccgcatt ggaggtgact ctggcctctc
     1081 agctcggggc aagcagtatg cctatgccct ggccaacttc attcggtctc aaagcatcag
     1141 ctccctgaag gtgtggacta gccacatgaa gaggaccatt cagacagctg aagccctagg
     1201 cgtcccctat gaacagtgga aggccctgaa tgagattgat gcgggtgtct gtgaagagat
     1261 gacttatgaa gaaattcagg aacactaccc tgaagaattt gcactacggg accaggataa
     1321 atatcgttac cgctatccca agggagagtc ctatgaggat ctggttcagc gtcttgagcc
     1381 agttataatg gagctagaac ggcaagaaaa tgtactggtg atctgtcacc aggctgtcat
     1441 gcggtgcctt ctagcatact tcctggataa aagttcagat gagctgccct atctcaagtg
     1501 tcctctgcat acagtgctca aactcacacc tgtggcttat ggctgcagag tggagtccat
     1561 ctacctgaat gtggaggctg tgaacacaca ccgggacaag cctgagaatg tggacatcac
     1621 ccgtgaaccc gaggaagccc tggacactgt ccctgcccat tactgagccc ttttcaagtg
     1681 atcagattgt ctctgttctc actaccttcc tcctgtagga actgctggcc ttgttctcct
     1741 taagcagact ctggctttgg cctgagtgtg ccctacctcc agagaataag tttgtagcag
     1801 ctcccaaaca ggtctcaatt cctagccata actaagccta cctagttgtg gataaagttc
     1861 tttttaaata ttctctgatg aataaagaca tttattcctg cctgtaggac aggaaaaagt
     1921 ta
//
