LOCUS       NM_011070               1730 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus prefoldin 2 (Pfdn2), transcript variant 1, mRNA.
ACCESSION   NM_011070
VERSION     NM_011070.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Chintalapudi,S.R., Morales-Tirado,V.M., Williams,R.W. and
            Jablonski,M.M.
  TITLE     Multipronged approach to identify and validate a novel upstream
            regulator of Sncg in mouse retinal ganglion cells
  JOURNAL   FEBS J 283 (4), 678-693 (2016)
   PUBMED   26663874
  REMARK    GeneRIF: Knocking down prefoldin-2 (Pfdn2) expression in primary
            murine retinal ganglion cells(RGCs) significantly reduced Sncg
            expression, confirming that Pfdn2 regulates Sncg expression in
            murine RGCs.
REFERENCE   3  (bases 1 to 1730)
  AUTHORS   Eddleston,J., Murdoch,J.N., Copp,A.J. and Stanier,P.
  TITLE     Physical and transcriptional map of a 3-Mb region of mouse
            chromosome 1 containing the gene for the neural tube defect mutant
            loop-tail (Lp)
  JOURNAL   Genomics 56 (2), 149-159 (1999)
   PUBMED   10051400
REFERENCE   4  (bases 1 to 1730)
  AUTHORS   Vainberg,I.E., Lewis,S.A., Rommelaere,H., Ampe,C.,
            Vandekerckhove,J., Klein,H.L. and Cowan,N.J.
  TITLE     Prefoldin, a chaperone that delivers unfolded proteins to cytosolic
            chaperonin
  JOURNAL   Cell 93 (5), 863-873 (1998)
   PUBMED   9630229
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC087229.27.
            
            On Feb 15, 2018 this sequence version replaced NM_011070.3.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.3554099.1,
                                           SRR7652917.242608.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               AC087229.27        91047-91158
            113-201             AC087229.27        101902-101990
            202-325             AC087229.27        102077-102200
            326-1730            AC087229.27        103222-104626
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 79.35 cM"
     gene            1..1730
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
                     /note="prefoldin 2"
                     /db_xref="GeneID:18637"
                     /db_xref="MGI:MGI:1276111"
     exon            1..112
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..502
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     prefoldin subunit 2"
                     /codon_start=1
                     /product="prefoldin subunit 2 isoform 1"
                     /protein_id="NP_035200.2"
                     /db_xref="CCDS:CCDS15491.1"
                     /db_xref="GeneID:18637"
                     /db_xref="MGI:MGI:1276111"
                     /translation="MADSSGRVGKSGGSGAGKGAVSAEQVIAGFNRLRQEQRGLASKA
                     AELEMELNEHSLVIDTLKEVDETRKCYRMVGGVLVERTVKEVLPALEGNKEQIQKIIE
                     TLSQQLQAKGKELNEFREKHNIRLMGEDEKPAAKENSEGAGAKASSAGVLVS"
     exon            113..201
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
                     /inference="alignment:Splign:2.1.0"
     exon            202..325
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
                     /inference="alignment:Splign:2.1.0"
     exon            326..1730
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
                     /inference="alignment:Splign:2.1.0"
     regulatory      622..627
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
                     /note="hexamer: ATTAAA"
     polyA_site      646
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
                     /note="major polyA site"
     regulatory      1711..1716
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
                     /note="hexamer: ATTAAA"
     polyA_site      1730
                     /gene="Pfdn2"
                     /gene_synonym="ESTM27"
ORIGIN      
        1 gagagtgcgc aggccggaaa gcttgcaggt ggggaagatg gcggacagca gcggtcgtgt
       61 gggcaagagc ggcgggagcg gcgcggggaa gggggcggtg tcggcggaac aggtaattgc
      121 tggctttaat cgccttcggc aggaacagag gggcttggct tccaaagcag ctgagctgga
      181 gatggaattg aatgagcaca gcctggtgat cgatacactg aaggaagtgg atgagacccg
      241 caagtgctac cgcatggttg gaggtgtgct ggtggagcgg actgtcaaag aagtgctgcc
      301 tgccttggag ggcaacaagg agcagataca gaagatcatt gagacactgt cacagcagct
      361 tcaggcaaag ggcaaagaac tgaacgaatt ccgggaaaag cacaacattc gtctcatggg
      421 ggaagatgag aagccagcag ccaaggaaaa ctcagaaggg gctggggcta aggccagctc
      481 agcaggggtg ttggtctctt aggagctaag gcctctgcgg gttttttttt ttttaccctg
      541 attcccactt gtaatttctt tttattgtcg ttattattat ttctctgcta ttgtaatatt
      601 ttgtttttgt tgttgttgtt gattaaatgt tttggtcaaa tacttagcta tagcctgaac
      661 ctttatctca tttagggcta caccgacctc atagagctgg gtgtggtgcc acacatgcct
      721 ttaataatct tagtgcttgg gtgacaacaa gaggcagatc tcggagttcc aggccagcct
      781 ggtctacaaa tcgagttccc agtaatccaa ggctatatat agacacagtg agaccctgtc
      841 tcaaacaaaa caaaaaaata tgatttgcat tgtttctacc aggttaatgg ctcactatca
      901 gaaaggaacc tcagttcggt ctttcctttg caaataaagt aatctgttta tttgatgctc
      961 ttctaaaacg gattcagagg gctggagaga tggctcagat ggctcaacag tgaagagctc
     1021 tgtctgctct tccagggggc atctgtttta ttatccccaa caaccacatg gtggctcaca
     1081 accatctata atggggtctg atgccctctt ctggcacaaa ggtgtacatg caaatagggc
     1141 acttatataa gtaaaatgta taaatcttct aaaggattca gagacaaaac tctcggattg
     1201 aaatttaata agtatttgaa gtagggcaat agctctgatg agatcgcttt gtaggtttgc
     1261 cacaacacag agtgcctccc tgctgttctt tagatgaaga attccaggat gcaaacctgt
     1321 gggctagctg tcctgccgtc ctcaccaaaa cccccaacca aataaagctg gtcattccaa
     1381 aggcaagtcc gatggcccac gcgcttcagt ccacggtctg tactggggaa gtggaaccac
     1441 agaggcggag acttgcctgt gggttgagag cagaaggtgg ttactgtggc attctgctcc
     1501 ggccttaccc agtacgcatg cctgtcatcg ttcccgtttg atttgtcagc cttttcagat
     1561 gttcagtatt tatattgtct attgtatttt gtttttgaaa cagggtctca ctctgtcacc
     1621 ctggctagtt tcacactcta gatcaagttg gctggcctca aaaactacca gagatctgcc
     1681 tgcctctgcc tctgcctccc aagtgctgga attaaaagtg tgaatcacta
//
