LOCUS       NM_001159519            6369 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus phosphoribosylformylglycinamidine synthase (FGAR
            amidotransferase) (Pfas), mRNA.
ACCESSION   NM_001159519 XM_111232 XM_908501
VERSION     NM_001159519.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Mizukoshi,T., Yamada,S. and Sakakibara,S.I.
  TITLE     Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis
            during Brain Development
  JOURNAL   eNeuro 10 (10) (2023)
   PUBMED   37770184
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6369)
  AUTHORS   Mangold,C.A., Yao,P.J., Du,M., Freeman,W.M., Benkovic,S.J. and
            Szpara,M.L.
  TITLE     Expression of the purine biosynthetic enzyme phosphoribosyl
            formylglycinamidine synthase in neurons
  JOURNAL   J Neurochem 144 (6), 723-735 (2018)
   PUBMED   29337348
  REMARK    GeneRIF: Study observed FGAMS as distinct puncta throughout
            neuronal processes, where it co-localized with neurofilament heavy
            chain, tubulin, and mitochondria. Data provide insight into
            potential purine biosynthetic mechanisms utilized within neurons
            during homeostasis as well as viral infection.
REFERENCE   4  (bases 1 to 6369)
  AUTHORS   Baresova,V., Krijt,M., Skopova,V., Souckova,O., Kmoch,S. and
            Zikanova,M.
  TITLE     CRISPR-Cas9 induced mutations along de novo purine synthesis in
            HeLa cells result in accumulation of individual enzyme substrates
            and affect purinosome formation
  JOURNAL   Mol Genet Metab 119 (3), 270-277 (2016)
   PUBMED   27590927
REFERENCE   5  (bases 1 to 6369)
  AUTHORS   Palmer,K., Fairfield,H., Borgeia,S., Curtain,M., Hassan,M.G.,
            Dionne,L., Yong Karst,S., Coombs,H., Bronson,R.T., Reinholdt,L.G.,
            Bergstrom,D.E., Donahue,L.R., Cox,T.C. and Murray,S.A.
  TITLE     Discovery and characterization of spontaneous mouse models of
            craniofacial dysmorphology
  JOURNAL   Dev Biol 415 (2), 216-227 (2016)
   PUBMED   26234751
REFERENCE   6  (bases 1 to 6369)
  AUTHORS   Fairfield,H., Srivastava,A., Ananda,G., Liu,R., Kircher,M.,
            Lakshminarayana,A., Harris,B.S., Karst,S.Y., Dionne,L.A.,
            Kane,C.C., Curtain,M., Berry,M.L., Ward-Bailey,P.F., Greenstein,I.,
            Byers,C., Czechanski,A., Sharp,J., Palmer,K., Gudis,P., Martin,W.,
            Tadenev,A., Bogdanik,L., Pratt,C.H., Chang,B., Schroeder,D.G.,
            Cox,G.A., Cliften,P., Milbrandt,J., Murray,S., Burgess,R.,
            Bergstrom,D.E., Donahue,L.R., Hamamy,H., Masri,A., Santoni,F.A.,
            Makrythanasis,P., Antonarakis,S.E., Shendure,J. and Reinholdt,L.G.
  TITLE     Exome sequencing reveals pathogenic mutations in 91 strains of mice
            with Mendelian disorders
  JOURNAL   Genome Res 25 (7), 948-957 (2015)
   PUBMED   25917818
REFERENCE   7  (bases 1 to 6369)
  AUTHORS   Fairfield,H., Gilbert,G.J., Barter,M., Corrigan,R.R., Curtain,M.,
            Ding,Y., D'Ascenzo,M., Gerhardt,D.J., He,C., Huang,W., Richmond,T.,
            Rowe,L., Probst,F.J., Bergstrom,D.E., Murray,S.A., Bult,C.,
            Richardson,J., Kile,B.T., Gut,I., Hager,J., Sigurdsson,S.,
            Mauceli,E., Di Palma,F., Lindblad-Toh,K., Cunningham,M.L.,
            Cox,T.C., Justice,M.J., Spector,M.S., Lowe,S.W., Albert,T.,
            Donahue,L.R., Jeddeloh,J., Shendure,J. and Reinholdt,L.G.
  TITLE     Mutation discovery in mice by whole exome sequencing
  JOURNAL   Genome Biol 12 (9), R86 (2011)
   PUBMED   21917142
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 6369)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   9  (bases 1 to 6369)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645902.6 and AL714000.10.
            
            On or before Apr 19, 2009 this sequence version replaced
            XM_908501.3, XM_111232.8.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253014.2699922.1,
                                           SRR17253011.10124058.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                AL645902.6         23644-23701         c
            59-235              AL645902.6         18937-19113         c
            236-371             AL645902.6         17721-17856         c
            372-477             AL645902.6         16974-17079         c
            478-667             AL645902.6         16348-16537         c
            668-773             AL645902.6         16115-16220         c
            774-914             AL645902.6         15690-15830         c
            915-1039            AL645902.6         15486-15610         c
            1040-1168           AL645902.6         15250-15378         c
            1169-1300           AL645902.6         13154-13285         c
            1301-1429           AL645902.6         12905-13033         c
            1430-1503           AL645902.6         9499-9572           c
            1504-1660           AL645902.6         9049-9205           c
            1661-1722           AL645902.6         8807-8868           c
            1723-1884           AL645902.6         8538-8699           c
            1885-2007           AL645902.6         8157-8279           c
            2008-2139           AL645902.6         7896-8027           c
            2140-2370           AL645902.6         7421-7651           c
            2371-2551           AL645902.6         7097-7277           c
            2552-2635           AL645902.6         6926-7009           c
            2636-2791           AL645902.6         6579-6734           c
            2792-2911           AL645902.6         6281-6400           c
            2912-3057           AL645902.6         5914-6059           c
            3058-3265           AL645902.6         5609-5816           c
            3266-3357           AL645902.6         5411-5502           c
            3358-3472           AL645902.6         5212-5326           c
            3473-3796           AL645902.6         3608-3931           c
            3797-5310           AL645902.6         2001-3514           c
            5311-6369           AL714000.10        2936-3994           c
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 42.26 cM"
     gene            1..6369
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /note="phosphoribosylformylglycinamidine synthase (FGAR
                     amidotransferase)"
                     /db_xref="GeneID:237823"
                     /db_xref="MGI:MGI:2684864"
     exon            1..58
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            59..235
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..4107
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /EC_number="6.3.5.3"
                     /note="FGAM synthase; formylglycinamide ribotide
                     synthetase; formylglycinamide ribotide amidotransferase;
                     FGAR amidotransferase; formylglycinamide ribonucleotide
                     amidotransferase"
                     /codon_start=1
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="NP_001152991.1"
                     /db_xref="CCDS:CCDS48823.1"
                     /db_xref="GeneID:237823"
                     /db_xref="MGI:MGI:2684864"
                     /translation="MAPVLHFYVRPSGHEGAASGRVFRRLQEKLPTLQSVETELCYNV
                     HWAAETLPWAEEMKKLMWLFGCPLVRDDVAQEPWLVPGSNDLLLEVGPRLNFSTPAST
                     NIVSVCQAAGLRAVDRVETTRRYRLSFTDHPTAEMEAISLAALHDRMTEQHYPDPIQS
                     FSPQSIPAPLKGSIDILAEGRPALEKANQELGLALDSWDLDFYTKRFQELQRNPSTVE
                     VFDLAQSNSEHSRHWFFKGQLHVDGKKLAHSLFESIMSTQASSNPNNVLKFCDNSSAI
                     QGKKVKFLRPEDSTRPSCFQQQQGLRHVVFTAETHNFPTGVAPFSGATTGTGGRIRDV
                     QCTGRGAHVVAGTAGYCFGNLHIPDYNLPWEDPSFQYPGNFARPLEVAIEASNGASDY
                     GNKFGEPVLAGFARSLGLQLPDGQRREWIKPIMFSGGIGSMEAKHVGKKPPEPGMEVV
                     KVGGPVYRIGVGGGAASSVQVQGDNTSDLDFGAVQRGDPEMEQKMNRVIRACVEAPGG
                     NPICSLHDQGAGGNGNVLKELSDPEGAIIYTSRFQLGDPTLNALEIWGAEYQESNALL
                     LRPSDRDFLSRASARERCPACFVGTITGDKRIVLVDDRECLVGKTGQGDAPLTPPTPV
                     DLDLDWVLGKMPQKEFFLQRKPPVLQPLALPPELSVRQALNRVLRLPAVASKRYLTNK
                     VDRSVGGLVAQQQCVGPLQTPLADVAVVALSHQECIGAATALGEQPVKSLLDPKAAAR
                     LAVSEALTNLVFALVTDLRDVKCSGNWMWAAKLPGEGAALADACEAMVAVMAALGVAV
                     DGGKDSLSMAARVGTETVQAPGSLVISAYAVCPDITATVTPDLKHPGGKGHLLYVPLS
                     PGQHRLGGTALAQCFSQLGEHPPDLDLPENLVRAFHITQGLLKECRLCSGHDVSDGGL
                     VTCLLEMAFAGNCGIEVDVPAPGIHALPVLFAEEPGLVLEVQEADVAGVRQRYESAGL
                     RCLELGHTGEAGPQAMARISVNKAVVVEEPVGELRALWEETSFQLDLLQAEPRCVIEE
                     KQGLKERTGPSYYLPPTFPVASVPCKPGGPVPRVAILREEGSNGDREMADAFHLAGFE
                     VWDVTMQDLCSGAIRLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFNPQAREELGRFR
                     RRPDTFSLGVCNGCQLLALLGWVGSDPSEEQAEPGQDSQPTQPGLLLRHNLSGRFESR
                     WATVRVEPGPALMLRGMEGSVLPVWSAHGEGYMAFSSPELQAKIEAKGLVPLHWADDD
                     GNPTEQYPLNPNGSPGGIAGICSQDGRHLALMPHPERAVRLWQWAWRPSPFDVLPTSP
                     WLQLFINARNWTQEDSC"
     exon            236..371
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            372..477
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            478..667
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            668..773
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            774..914
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1039
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1168
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1300
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1429
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1503
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1660
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1722
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1884
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2007
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2139
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            2140..2370
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            2371..2551
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2635
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            2636..2791
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            2792..2911
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            2912..3057
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            3058..3265
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            3266..3357
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            3358..3472
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            3473..3796
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     exon            3797..6369
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5594..5599
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /note="hexamer: ATTAAA"
     polyA_site      5617
                     /gene="Pfas"
                     /gene_synonym="4432409B16Rik; FGAMS; FGAR-AT; FGARAT;
                     Gm18; PURL; Sofa"
                     /note="major polyA site"
ORIGIN      
        1 gtacaacgag cgcgcgccaa ggactcgtgg gtctctggcg caaactggcg ggcttcagga
       61 gccaaattct tttctaaaag gtcatctgca gaaatggccc ctgtccttca cttttatgtc
      121 cgcccctctg gccatgaggg ggcagcatct ggacgtgttt ttcggagact acaagagaaa
      181 ctgccaacac ttcagagtgt ggagactgag ctgtgctaca atgtacactg ggcagctgag
      241 acccttccat gggctgagga gatgaagaag ttgatgtggc tgttcggctg ccctttggtg
      301 cgggatgatg ttgctcagga gccctggctt gttcctggct ccaacgattt gttgctggag
      361 gtgggaccca ggctgaattt ctcgactcca gcatcgacca acattgtttc tgtgtgccag
      421 gcagccgggc tgagggctgt ggatcgggta gagaccaccc gacgttaccg actttcgttt
      481 accgaccacc ctacagctga aatggaagcc atctctctgg ccgccttgca cgaccggatg
      541 acagagcagc actaccctga ccccatccag agcttctctc ctcagagcat tccagcccca
      601 ctcaagggct ccatcgacat actagctgag ggtcggcctg ccctggagaa ggccaaccag
      661 gagctaggtt tggctcttga ctcctgggac ctcgatttct ataccaagcg ctttcaagag
      721 ctgcagcgga accccagtac agtggaggtc tttgacttgg ctcagtccaa tagtgagcac
      781 agccgacatt ggttcttcaa gggccagctc catgtggatg ggaagaagct agcgcattcc
      841 ctctttgaat ccatcatgag cactcaggca tcctccaacc ctaacaatgt cctcaagttc
      901 tgtgacaaca gcagtgcaat ccaggggaag aaagtcaaat tcctgcggcc ggaggactct
      961 acccgcccaa gctgcttcca gcaacagcag gggcttagac atgttgtctt cacagcagag
     1021 acacataact tccccacagg agtggccccc ttcagcggtg caaccacagg cacgggtggc
     1081 cgcatcagag acgtccagtg cacaggccgt ggggcccacg tggtagctgg cactgctggc
     1141 tattgctttg gaaacctgca catcccagat tataatctgc cctgggagga cccaagcttc
     1201 cagtatcctg ggaactttgc caggcctttg gaggttgcta ttgaggccag caatggagca
     1261 tctgactatg gcaacaagtt tggagagcca gtgctggctg gatttgcccg ctctttgggt
     1321 ctccagcttc cagatggtca gagacgtgag tggatcaagc ccatcatgtt cagtggcggc
     1381 attgggtcca tggaagccaa acatgtaggc aaaaagcccc cagagccagg catggaggtt
     1441 gtaaaggttg gaggtcctgt ttataggatt ggagttgggg gtggagctgc ttcgtctgtg
     1501 caggtccagg gagacaacac cagtgacctg gactttggag ctgtgcaacg gggagacccg
     1561 gaaatggagc aaaagatgaa tcgtgtgatc cgggcttgtg tggaggcccc aggaggaaac
     1621 cccatctgca gcctccatga ccagggtgct ggtggcaatg gcaatgtcct aaaggagctg
     1681 agtgacccag aaggagccat catttacacc agccgcttcc agctaggtga cccaaccttg
     1741 aatgcactgg aaatctgggg ggctgagtac caggagtcaa atgcactact gcttaggccc
     1801 tctgaccggg acttcctgag tcgtgccagt gcccgggaac gctgcccagc ttgttttgtg
     1861 ggcacaatca ctggagacaa gagaattgta ctagtggatg atcgtgaatg cctagtagga
     1921 aaaactggtc agggagatgc acccctgaca cctccaaccc ctgtagacct ggaccttgac
     1981 tgggtgctgg gaaagatgcc tcagaaggag ttcttcctcc agaggaaacc ccctgtgctg
     2041 cagcctttgg ctttgccccc agaactgagt gtgcgacagg ctctgaaccg ggtcctaagg
     2101 ctgcctgctg tggccagcaa acgctacctc accaacaagg tggatcgctc tgtgggtggc
     2161 cttgttgccc aacagcagtg tgtcggaccc ctgcagactc ctctggctga tgtggctgtt
     2221 gtagcactga gccaccagga gtgcataggg gctgccacag ccctgggaga gcagccagtc
     2281 aagagcctgc tggaccccaa ggctgctgcc aggcttgctg tgtctgaggc cctcaccaac
     2341 ctggtgtttg ctctggtcac cgacctccga gatgtgaagt gcagtgggaa ctggatgtgg
     2401 gcagccaagc tcccaggtga gggtgcagcg ctggctgatg cctgtgaggc tatggtggca
     2461 gtgatggcag cactgggtgt ggcagtagat ggtggcaaag actccctcag catggctgcc
     2521 cgggtcggca ctgagacggt gcaggctcct gggtcactgg tcatttcagc atatgctgtc
     2581 tgtccagaca tcacagccac tgtgacccca gacctcaaac atcctggtgg gaaaggtcat
     2641 ttgctttatg tgcctttgag ccccgggcag caccggctgg gaggaacggc tctagcccag
     2701 tgtttctccc agcttgggga gcaccctcct gatctagacc ttcccgagaa ccttgtgcgt
     2761 gcctttcata tcacccaggg gctgctgaaa gaatgccgtc tttgctcagg ccatgatgtc
     2821 agcgatgggg ggcttgtcac ctgccttcta gagatggcct ttgctgggaa ctgtgggata
     2881 gaggtagatg tacctgcccc tgggatccat gcattgcctg tgctgtttgc ggaagagccc
     2941 ggtctggtgt tggaggtaca ggaggcagat gtggccggag tacggcagag gtacgagagt
     3001 gctggcctac ggtgtctgga actgggccac acaggcgagg ctggacccca ggccatggcc
     3061 cgaatatcag tgaataaggc tgtggtagta gaggagcctg ttggggagtt aagagccctc
     3121 tgggaggaaa caagtttcca gctggaccta ctgcaggcag agccacgctg tgtgatagag
     3181 gagaaacagg gcctgaagga gcggacaggg cccagctact acctgccgcc cacctttcct
     3241 gtggcctctg tgccctgtaa acctggtgga cccgtccctc gagttgccat cttgcgagaa
     3301 gagggcagca atggagaccg agaaatggct gatgcctttc acttggcagg ctttgaggtg
     3361 tgggatgtga ccatgcaaga tctctgctct ggggccatca ggttggacac attccgtggt
     3421 gtggcctttg tgggcggctt cagctacgca gacgtcctgg gctctgctaa aggttgggca
     3481 gctgcggtga cctttaatcc acaggccagg gaagagctgg gccgattccg aaggcggccg
     3541 gacaccttca gcctgggcgt gtgtaatggc tgtcagctgc tggctcttct gggctgggtg
     3601 ggaagtgatc ccagtgaaga gcaagctgag ccaggccagg actcccagcc aacacagccg
     3661 ggcctcctgc tccgccacaa tctgtctggg cggttcgagt cccgatgggc caccgtacgt
     3721 gtggagccgg ggccagccct gatgctccga gggatggaag gctctgtgct gcccgtgtgg
     3781 agtgctcacg gggaaggcta catggcattt tcttctcctg aactccaagc caagatcgag
     3841 gccaagggcc tggttcctct gcactgggca gacgatgatg ggaaccccac agagcagtac
     3901 cctctgaacc ccaatggatc tccagggggc atagctggta tctgttccca agatggccga
     3961 cacctggcac tcatgccgca ccctgagcgg gctgtaaggc tttggcagtg ggcatggcga
     4021 ccctctccct ttgatgttct tcccacctcc ccgtggctgc agctcttcat caatgctagg
     4081 aactggaccc aggaggacag ctgctgagga ggccgcatga ccaccctggc ccacagctca
     4141 ccctttgact ccgttgcctt ctttccccat tgctttcagg agtatcccat gtgatttcca
     4201 aaagcatctt agcaagaacc aaaatgctgg gatgacctca gctgcattgc tcattttagc
     4261 cttgcccctg caagagctgc tttcagctct tgagtttaac atggatcttc agcaagcggc
     4321 tcaggctccg aggacagctg acaggcagag gggtgggcag agatttactg gcagccctcc
     4381 cgggatcatc gccttgtgat ccttcattct cttggttaga gctctgagtg agctctcaca
     4441 tcctcgtttt ctttggcttt gtgagatcgg ctttcagtct atagcccagg ctggccctaa
     4501 actcagaatt cttttgcgtt accccgggtg agttgccaag gcgcttggca ttggcttagg
     4561 gtctaggaat aatgtacatc agtccctccc agcctctgcc cagctgaaaa gagcagcttt
     4621 ccggatccct tttcagacat gcactgctag ttgttaggaa tcattgcttc tgtccatgct
     4681 caaggcgctg acctgtgcct atctcagatc acattcctga ttcttgcttc tcttaattta
     4741 gcagccacct tacagtagtt gataccttcc ttcttagagc agagagccct ggagcttgtt
     4801 ttttgttgtt ttggcttttc aagacagggt tgggtttttg tttttgtttt tgttttttgg
     4861 gtttttttgt tttgttttgt ttttatgtgt gtatagccct ggctgtcctg gaacttatgt
     4921 tgtagaccgg ctggcctcgc actcagagat ccatctgagt gctggggtta aggcatgtgc
     4981 gactactgcc tggccattca tttttcttct tgttgcttga tcagtagtgg tgcctgacat
     5041 tggtactgac ctaatggagt ttgtagtctt tttttttttt tttttttttt ttttttggtt
     5101 tggtttggtt tcaggcaagg tctctctgtg tagccctggc tgtcctggaa ctcactttgt
     5161 agaccaggct ggcctcgaac tcagaaatcc acctgcctct gcctcccgag tgctgggatt
     5221 aaaggcatgc gccaccacgc ccggcttgtt tgtagtctta ataggattga aataataata
     5281 gctccttagc ttgtactgcc aacctaaata ggtgctgggt aacttatgtg tctatcataa
     5341 agattaaggt atttgagagg tgtttcccag ttaacaaagc aaagtttagt gataaactgt
     5401 ctatgattgg ttccaactta aaatgaacag caaggcgccc tacccaccca gctacatctg
     5461 cctgtgaaga tcctgcagtc acagatcacg tcttggtagt agctgttgta ccgacagcca
     5521 ccttcatcat ccgttgcttc ctacttactt gtacggtaga ctcgggtagc cccctaagaa
     5581 gctcgatgtc atgattaaag gagtttcctt ataaaaacag actagcatgt tggatttcta
     5641 aatatttcag ggtttggggg aatgcagaac acttgtgctt ctgcagaagt agcaccccaa
     5701 gaaggtacta atggtgaata ggtgttaagc tgcaggttgt atgtccatct gagtattaga
     5761 acacttgcct agtatctgtg tttcctgagt ttgattcccg tccagcacca ccaaaggaag
     5821 atatttggag atgggaagtc agatctttct ccgttttgca ttgcaaagta acttttccta
     5881 actagagtga ttctaaggat tttgtttgta ttttcgagac ccatatgatg ttctggctgg
     5941 ctttgaactc atagctgaga gtaatcttga aatcctggtc ttctaccaag tgctgtgttc
     6001 tgacctaagt accattttgg acatgtgaac attccctttg atgctgtgga agaatatata
     6061 ttttgcttat ggtggtacat gccttttatc ccaacactta ggagataggg gcaggtggat
     6121 ctttgtaagt tcaaggccag cctagtgagt tccaggctag tcaggacact gtctcaaatt
     6181 tatatattta aagtatttat gaaagtattt ttgtaagcct gagtaggcaa gatggcttag
     6241 tgcagaagtt ttcaaccttc ctaatgctgt aaccctgtaa tacagttcct catgttattg
     6301 tgggcacaac cataaagttt tggttgctat tttataactg tagttttgct gctattaaaa
     6361 atcataatg
//
