LOCUS       NM_008819               1023 bp    mRNA    linear   ROD 14-MAY-2024
DEFINITION  Mus musculus phosphatidylethanolamine N-methyltransferase (Pemt),
            transcript variant 2, mRNA.
ACCESSION   NM_008819
VERSION     NM_008819.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Sun,C., Holstein,D.J.F., Garcia-Cubero,N., Moulla,Y., Stroh,C.,
            Dietrich,A., Schon,M.R., Gartner,D., Lohmann,T., Dressler,M.,
            Stumvoll,M., Bluher,M., Kovacs,P. and Guiu-Jurado,E.
  TITLE     The Role of Phosphatidylethanolamine N-Methyltransferase (PEMT) and
            Its Waist-Hip-Ratio-Associated Locus rs4646404 in Obesity-Related
            Metabolic Traits and Liver Disease
  JOURNAL   Int J Mol Sci 24 (23), 16850 (2023)
   PUBMED   38069170
  REMARK    GeneRIF: The Role of Phosphatidylethanolamine N-Methyltransferase
            (PEMT) and Its Waist-Hip-Ratio-Associated Locus rs4646404 in
            Obesity-Related Metabolic Traits and Liver Disease.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Trzaskalski,N.A., Vulesevic,B., Nguyen,M.A., Jeraj,N.,
            Fadzeyeva,E., Morrow,N.M., Locatelli,C.A., Travis,N., Hanson,A.A.,
            Nunes,J.R., O'Dwyer,C., van der Veen,J.N., Lorenzen-Schmidt,I.,
            Seymour,R., Pulente,S.M., Clement,A.C., Crawley,A.M., Jacobs,R.L.,
            Doyle,M.A., Cooper,C.L., Kim,K.H., Fullerton,M.D. and
            Mulvihill,E.E.
  TITLE     Hepatocyte-derived DPP4 regulates portal GLP-1 bioactivity,
            modulates glucose production, and when absent influences NAFLD
            progression
  JOURNAL   JCI Insight 8 (2), e154314 (2023)
   PUBMED   36472923
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1023)
  AUTHORS   Zhou,Q., Zhang,F., Kerbl-Knapp,J., Korbelius,M., Kuentzel,K.B.,
            Vujic,N., Akhmetshina,A., Horl,G., Paar,M., Steyrer,E., Kratky,D.
            and Madl,T.
  TITLE     Phosphatidylethanolamine N-Methyltransferase Knockout Modulates
            Metabolic Changes in Aging Mice
  JOURNAL   Biomolecules 12 (9), 1270 (2022)
   PUBMED   36139111
  REMARK    GeneRIF: Phosphatidylethanolamine N-Methyltransferase Knockout
            Modulates Metabolic Changes in Aging Mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1023)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1023)
  AUTHORS   Sprenger,R.R., Hermansson,M., Neess,D., Becciolini,L.S.,
            Sorensen,S.B., Fagerberg,R., Ecker,J., Liebisch,G., Jensen,O.N.,
            Vance,D.E., Faergeman,N.J., Klemm,R.W. and Ejsing,C.S.
  TITLE     Lipid molecular timeline profiling reveals diurnal crosstalk
            between the liver and circulation
  JOURNAL   Cell Rep 34 (5), 108710 (2021)
   PUBMED   33535053
REFERENCE   6  (bases 1 to 1023)
  AUTHORS   Oki,T., Yamazaki,K., Kuromitsu,J., Okada,M. and Tanaka,I.
  TITLE     cDNA cloning and mapping of a novel subtype of
            glutamine:fructose-6-phosphate amidotransferase (GFAT2) in human
            and mouse
  JOURNAL   Genomics 57 (2), 227-234 (1999)
   PUBMED   10198162
REFERENCE   7  (bases 1 to 1023)
  AUTHORS   Walkey,C.J., Yu,L., Agellon,L.B. and Vance,D.E.
  TITLE     Biochemical and evolutionary significance of phospholipid
            methylation
  JOURNAL   J Biol Chem 273 (42), 27043-27046 (1998)
   PUBMED   9765216
REFERENCE   8  (bases 1 to 1023)
  AUTHORS   Walkey,C.J., Donohue,L.R., Bronson,R., Agellon,L.B. and Vance,D.E.
  TITLE     Disruption of the murine gene encoding phosphatidylethanolamine
            N-methyltransferase
  JOURNAL   Proc Natl Acad Sci U S A 94 (24), 12880-12885 (1997)
   PUBMED   9371769
REFERENCE   9  (bases 1 to 1023)
  AUTHORS   Yang,Z., Hanlon,D.W., Marszalek,J.R. and Goldstein,L.S.
  TITLE     Identification, partial characterization, and genetic mapping of
            kinesin-like protein genes in mouse
  JOURNAL   Genomics 45 (1), 123-131 (1997)
   PUBMED   9339368
REFERENCE   10 (bases 1 to 1023)
  AUTHORS   Walkey,C.J., Cui,Z., Agellon,L.B. and Vance,D.E.
  TITLE     Characterization of the murine phosphatidylethanolamine
            N-methyltransferase-2 gene
  JOURNAL   J Lipid Res 37 (11), 2341-2350 (1996)
   PUBMED   8978486
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645526.14, AY334571.1, AK133744.1 and AA245746.1.
            
            On Feb 20, 2014 this sequence version replaced NM_008819.2.
            
            Transcript Variant: This variant (2) has an alternate 5' terminal
            exon and uses a downstream start codon compared to variant 1. It
            encodes isoform b which has a shorter N-terminus compared to
            isoform a. Variants 2, 3 and 4 encode the same isoform (b).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026796.1, AY334571.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               AL645526.14        17361-17501         c
            142-783             AY334571.1         1-642
            784-999             AK133744.1         746-961
            1000-1023           AA245746.1         244-267
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 37.81 cM"
     gene            1..1023
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /note="phosphatidylethanolamine N-methyltransferase"
                     /db_xref="GeneID:18618"
                     /db_xref="MGI:MGI:104535"
     exon            1..184
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /inference="alignment:Splign:2.1.0"
     exon            185..292
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..799
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /EC_number="2.1.1.17"
                     /EC_number="2.1.1.71"
                     /note="isoform b is encoded by transcript variant 2;
                     phospholipid methyltransferase"
                     /codon_start=1
                     /product="phosphatidylethanolamine N-methyltransferase
                     isoform b"
                     /protein_id="NP_032845.2"
                     /db_xref="CCDS:CCDS24782.1"
                     /db_xref="GeneID:18618"
                     /db_xref="MGI:MGI:104535"
                     /translation="MSWLLGYMDPTEPSFVAAVITIVFNPLFWNVVARWEQRTRKLSR
                     AFGSPHLACYSLGICILLLNILRSHCFTQAMMSQPKMEGLDNHTTYFLGLAFLGWGFV
                     FVLSSFYALGFTGTFLGDYFGILKESRVTTFPFSVLDNPMYWGSTANYLGWALMHASP
                     TGLLLTVLVAIVYVVALLYEEPFTAEIYRQKATRLHKRS"
     exon            293..408
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /inference="alignment:Splign:2.1.0"
     exon            409..554
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /inference="alignment:Splign:2.1.0"
     exon            555..666
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /inference="alignment:Splign:2.1.0"
     exon            667..741
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /inference="alignment:Splign:2.1.0"
     exon            742..1003
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      980..985
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /note="hexamer: AATAAA"
     polyA_site      1000
                     /gene="Pemt"
                     /gene_synonym="PEAMT; Pempt; Pempt2; PEMT2; PLMT"
                     /note="major polyA site"
ORIGIN      
        1 gttgaccccc tgcaaatcat tccctaacta cagaacattg ctctgaagtg tgtccaggtg
       61 cccagtgtgg tagaactgct atgtgccgcc ttcctttcag cttgtggggg ttctgcagct
      121 gagttcatca tcagggtgac ctgagctgtc cctggaggct ggctcccatt tccttctggt
      181 tctggctgat ctcttcgtta tgagctggct gctgggttac atggacccca cagagcccag
      241 ctttgtggcg gctgtgatca ccattgtgtt caacccactc ttctggaatg tggtagcgag
      301 atgggagcag agaactcgga agctgagcag agccttcggc tccccccacc tagcctgcta
      361 ttccctgggc atctgcatcc tgcttttgaa catcctccgc tcccactgct tcacacaggc
      421 tatgatgagc cagcccaaga tggagggcct ggacaaccac accacctact tcctgggcct
      481 tgcattccta ggttggggat tcgtgtttgt gctgtccagc ttctatgcac tggggttcac
      541 tgggaccttt ctaggtgact actttgggat cctcaaggag tccagagtga ccacatttcc
      601 cttcagcgtg ctggataacc ccatgtactg gggcagtaca gccaactacc taggctgggc
      661 actcatgcat gctagtccca caggcctgct gttgacggtg ctggtggcaa ttgtctacgt
      721 ggttgctctc ctatatgaag agcccttcac tgctgagatc taccgacaga aagctaccag
      781 gttgcacaaa agaagctgac agggccatga aggatctttg gaaagctgga ctggcctctc
      841 ggctgcccca agcaacaacc cttcccaggg agagcagcac tggccactgt acctgtgcct
      901 tagaaaccag tcatgggggc ctcaggcatt atgctgtgtg actgctgaga cccccatccc
      961 caccatgtcc cgacacacta ataaaggcat tgtgacctca gcaaaaaaaa aaaaaaaaaa
     1021 aaa
//
