LOCUS       NM_130877               6677 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus paternally expressed 10 (Peg10), transcript variant 1,
            mRNA.
ACCESSION   NM_130877 XM_132985
VERSION     NM_130877.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   McRae,H.M., Leong,M.P.Y., Bergamasco,M.I., Garnham,A.L., Hu,Y.,
            Corbett,M.A., Whitehead,L., El-Saafin,F., Sheikh,B.N., Wilcox,S.,
            Hannan,A.J., Gecz,J., Smyth,G.K., Thomas,T. and Voss,A.K.
  TITLE     Loss of PHF6 causes spontaneous seizures, enlarged brain ventricles
            and altered transcription in the cortex of a mouse model of the
            Borjeson-Forssman-Lehmann intellectual disability syndrome
  JOURNAL   PLoS Genet 20 (10), e1011428 (2024)
   PUBMED   39405291
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Tixi,W., Maldonado,M., Chang,Y.T., Chiu,A., Yeung,W., Parveen,N.,
            Nelson,M.S., Hart,R., Wang,S., Hsu,W.J., Fueger,P., Kopp,J.L.,
            Huising,M.O., Dhawan,S. and Shih,H.P.
  TITLE     Coordination between ECM and cell-cell adhesion regulates the
            development of islet aggregation, architecture, and functional
            maturation
  JOURNAL   Elife 12, e90006 (2023)
   PUBMED   37610090
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6677)
  AUTHORS   Bohuslavova,R., Fabriciova,V., Lebron-Mora,L., Malfatti,J.,
            Smolik,O., Valihrach,L., Benesova,S., Zucha,D., Berkova,Z.,
            Saudek,F., Evans,S.M. and Pavlinkova,G.
  TITLE     ISL1 controls pancreatic alpha cell fate and beta cell maturation
  JOURNAL   Cell Biosci 13 (1), 53 (2023)
   PUBMED   36899442
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6677)
  AUTHORS   Clark,M.B., Janicke,M., Gottesbuhren,U., Kleffmann,T., Legge,M.,
            Poole,E.S. and Tate,W.P.
  TITLE     Mammalian gene PEG10 expresses two reading frames by high
            efficiency -1 frameshifting in embryonic-associated tissues
  JOURNAL   J Biol Chem 282 (52), 37359-37369 (2007)
   PUBMED   17942406
  REMARK    GeneRIF: the ORF1-2 protein of PEG10, synthesized utilizing the
            most efficient -1 frameshift mechanism yet documented in vivo, will
            have an essential function that is intrinsic to the importance of
            PEG10 in mammals
REFERENCE   5  (bases 1 to 6677)
  AUTHORS   Ono,R., Nakamura,K., Inoue,K., Naruse,M., Usami,T.,
            Wakisaka-Saito,N., Hino,T., Suzuki-Migishima,R., Ogonuki,N.,
            Miki,H., Kohda,T., Ogura,A., Yokoyama,M., Kaneko-Ishino,T. and
            Ishino,F.
  TITLE     Deletion of Peg10, an imprinted gene acquired from a
            retrotransposon, causes early embryonic lethality
  JOURNAL   Nat Genet 38 (1), 101-106 (2006)
   PUBMED   16341224
  REMARK    GeneRIF: Peg10 gene is imprinted, with preferential expression from
            the paternal allele, and its deletion results in early embryonic
            lethality.
REFERENCE   6  (bases 1 to 6677)
  AUTHORS   Manktelow,E., Shigemoto,K. and Brierley,I.
  TITLE     Characterization of the frameshift signal of Edr, a mammalian
            example of programmed -1 ribosomal frameshifting
  JOURNAL   Nucleic Acids Res 33 (5), 1553-1563 (2005)
   PUBMED   15767280
  REMARK    GeneRIF: Characterization of a RNA pseudoknot as a frameshift
            signal for Edr programmed -1 ribosomal frameshifting.
            GeneRIF: Programmed -1 ribosomal frameshifting in mouse and human
            Peg10 genes.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6677)
  AUTHORS   Ono,R., Shiura,H., Aburatani,H., Kohda,T., Kaneko-Ishino,T. and
            Ishino,F.
  TITLE     Identification of a large novel imprinted gene cluster on mouse
            proximal chromosome 6
  JOURNAL   Genome Res 13 (7), 1696-1705 (2003)
   PUBMED   12840045
REFERENCE   8  (bases 1 to 6677)
  AUTHORS   Shigemoto,K., Brennan,J., Walls,E., Watson,C.J., Stott,D.,
            Rigby,P.W. and Reith,A.D.
  TITLE     Identification and characterisation of a developmentally regulated
            mammalian gene that utilises -1 programmed ribosomal frameshifting
  JOURNAL   Nucleic Acids Res 29 (19), 4079-4088 (2001)
   PUBMED   11574691
REFERENCE   9  (bases 1 to 6677)
  AUTHORS   Volff,J., Korting,C. and Schartl,M.
  TITLE     Ty3/Gypsy retrotransposon fossils in mammalian genomes: did they
            evolve into new cellular functions?
  JOURNAL   Mol Biol Evol 18 (2), 266-270 (2001)
   PUBMED   11158386
REFERENCE   10 (bases 1 to 6677)
  AUTHORS   Steplewski,A., Krynska,B., Tretiakova,A., Haas,S., Khalili,K. and
            Amini,S.
  TITLE     MyEF-3, a developmentally controlled brain-derived nuclear protein
            which specifically interacts with myelin basic protein proximal
            regulatory sequences
  JOURNAL   Biochem Biophys Res Commun 243 (1), 295-301 (1998)
   PUBMED   9473521
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ127881.1, AB091827.1,
            AC084315.4, CF735227.1 and BC094558.1.
            
            On May 18, 2006 this sequence version replaced NM_130877.1.
            
            Summary: This is a paternally expressed imprinted gene that is
            thought to have been derived from the Ty3/Gypsy family of
            retrotransposons. It contains two overlapping open reading frames,
            RF1 and RF2, and expresses two proteins: a shorter, gag-like
            protein (with a CCHC-type zinc finger domain) from RF1; and a
            longer, gag/pol-like fusion protein (with an additional aspartic
            protease motif) from RF1/RF2 by -1 translational frameshifting (-1
            FS). While -1 FS has been observed in RNA viruses and transposons
            in both prokaryotes and eukaryotes, this gene represents the first
            example of -1 FS in a eukaryotic cellular gene. This gene is highly
            conserved across mammalian species and retains the heptanucleotide
            (GGGAAAC) and pseudoknot elements required for -1 FS. It is
            expressed in adult and embryonic tissues (most notably in placenta)
            and reported to have a role in cell proliferation, differentiation,
            apoptosis and cancer development. Knockout mice lacking this gene
            showed early embryonic lethality with placental defects, indicating
            the importance of this gene in embryonic development. [provided by
            RefSeq, Oct 2014].
            
            Transcript Variant: This variant (1) has two overlapping open
            reading frames (RFI and RF2). This longer isoform (1) is derived
            from RF1/RF2 by -1 translational frameshifting.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CJ128728.1, BU519162.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            imprinted gene         :: PMID: 12840045
            RefSeq Select criteria :: based on expression, longest protein
            ribosomal slippage     :: PMID: 11574691, 17942406
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-74                CJ127881.1         1-74
            75-2461             AB091827.1         1-2387
            2462-2605           AC084315.4         19161-19304
            2606-3714           AB091827.1         2388-3496
            3715-4080           CF735227.1         296-661
            4081-6626           AB091827.1         3862-6407
            6627-6677           BC094558.1         3692-3742
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 1.81 cM"
     gene            1..6677
                     /gene="Peg10"
                     /gene_synonym="Edr; HB-1; Mar2; Mart2; MEF3L; MmPEG10;
                     MyEF-3; Rtl2"
                     /note="paternally expressed 10"
                     /db_xref="GeneID:170676"
                     /db_xref="MGI:MGI:2157785"
     exon            1..273
                     /gene="Peg10"
                     /gene_synonym="Edr; HB-1; Mar2; Mart2; MEF3L; MmPEG10;
                     MyEF-3; Rtl2"
                     /inference="alignment:Splign:2.1.0"
     exon            274..6667
                     /gene="Peg10"
                     /gene_synonym="Edr; HB-1; Mar2; Mart2; MEF3L; MmPEG10;
                     MyEF-3; Rtl2"
                     /inference="alignment:Splign:2.1.0"
     CDS             join(371..1480,1480..3390)
                     /gene="Peg10"
                     /gene_synonym="Edr; HB-1; Mar2; Mart2; MEF3L; MmPEG10;
                     MyEF-3; Rtl2"
                     /ribosomal_slippage
                     /note="protein translation is dependent on -1 ribosomal
                     frameshift; isoform 1 is encoded by transcript variant 1;
                     retrotransposon-derived protein PEG10; MEF3-like protein
                     1; ty3/Gypsy-like protein; myelin expression factor 3;
                     paternally expressed gene 10 protein; myelin expression
                     factor 3-like protein 1; mammalian retrotransposon-derived
                     protein 2; retrotransposon-derived gag-like polyprotein;
                     retrotransposon gag domain-containing protein 3; embryonal
                     carcinoma differentiation regulated protein; MyEF-3 like"
                     /codon_start=1
                     /product="retrotransposon-derived protein PEG10 isoform 1"
                     /protein_id="NP_570947.2"
                     /db_xref="GeneID:170676"
                     /db_xref="MGI:MGI:2157785"
                     /translation="MAAAGGSSNCPPPPPPPPPNNNNNNNTPKSPGVPDAEDDDERRH
                     DELPEDINNFDEDMNRQFENMNLLDQVELLAQSYSLLDHLDDFDDDDEDDDFDPEPDQ
                     DELPEYSDDDDLELQGAAAAPIPNFFSDDDCLEDLPEKFDGNPDMLGPFMYQCQLFME
                     KSTRDFSVDRIRVCFVTSMLIGRAARWATAKLQRCTYLMHNYTAFMMELKHVFEDPQR
                     REAAKRKIRRLRQGPGPVVDYSNAFQMIAQDLDWTEPALMDQFQEGLNPDIRAELSRQ
                     EAPKTLAALITACIHIERRLARDAAAKPDPSPRALVMPPNSQTDPTEPVGGARMRLSK
                     EEKERRRKMNLCLYCGNGGHFADTCPAKASKNSPPGKLPGPAVGGPSATGPERIRSPP
                     SEASTQHLQVMLQIHMPGRPTLFVRAMIDSGASGNFIDQDFVIQNAIPLRIKDWPVMV
                     EAIDGHPIASGPIILETHHLIVDLGDHREILSFDVTQSPFFPIVLGIRWLSTHDPHIT
                     WSTRSIVFNSDYCRLRCRMFAQIPSNLLFTVPQPNLHPYLLHHVHPHVHPHMHQHLHQ
                     HLHQFLHPDPHQYPHPDPHYHHHQQADMQHQLQQYLYQYLYYHLYPVMHHHLPPDQHE
                     HLHEYLHQYLHQYLHQFLHHHLHPDLHQYLYQYLHNHMNPDPHHHPHPDPPQDPHHPP
                     HQDPHQHPDPHQDPPHQDPHQHPDPHQDPPHQDPHQHPDPHQDPPHQDPHQDAHQHQD
                     PHQDAHQDPHQDAHQDPHMDPHLHQHQHPQPQPHPQQHPNHPQQPPFFYHMAGFRIYH
                     PVRYYYIQNVYTPVDEHVYPGHRVVDPNIEMIPGAHSLPSGHLYSMSESEMNALRNFV
                     DRNVKDGLMTPTVAPNGAQVLQVKRGWKLQVTYNCRAPQSGTIQNQYLRMSLPNMGDP
                     AHLASYGEFVQVPGYPYPAYVYYTSPHMMTAWYPVGRDVHGRIIVVPVVITWSQNTNR
                     QPPVPQYPPPQPPPPPPPPPPPPPPPPASSCSAA"
     regulatory      1489..1541
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Peg10"
                     /gene_synonym="Edr; HB-1; Mar2; Mart2; MEF3L; MmPEG10;
                     MyEF-3; Rtl2"
                     /note="recoding_pseudoknot"
                     /function="stimulates -1 ribosomal frameshifting"
     regulatory      6642..6647
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Peg10"
                     /gene_synonym="Edr; HB-1; Mar2; Mart2; MEF3L; MmPEG10;
                     MyEF-3; Rtl2"
                     /note="hexamer: AATAAA"
     polyA_site      6667
                     /gene="Peg10"
                     /gene_synonym="Edr; HB-1; Mar2; Mart2; MEF3L; MmPEG10;
                     MyEF-3; Rtl2"
                     /note="major polyA site"
ORIGIN      
        1 ggatttggcg ccccccctcc taggatctct ctatataagg caagcagtcc ggactcccga
       61 tacacgcgtt tccaacttga gttggtgtgt gtcgaagaat cctgaccaac tacgacctgg
      121 ggagagcagc caaccgagaa ggtccaccga gcctcgccta ggtctgctgc gcgggctgcg
      181 gtccggagcc ttctccggac cgcggtcacc cagtggaccg ggccgtcgcg ggacccctca
      241 tccttcgtgg catcgcagag gaatcctcgt gtggaacagg cgggttttaa gaaccaaaag
      301 acgccaacca cgagggtccc aggatccagg gctccctccc caggggagtg aagcccctct
      361 caccgcagcc atggctgctg cgggtggttc ctccaactgc ccgccccctc cccctccccc
      421 tcctcccaac aacaacaaca acaacaacac cccaaagagc ccaggcgtgc ctgacgccga
      481 agatgatgat gaacgcagac acgatgagct ccctgaagac atcaacaact ttgacgaaga
      541 catgaacagg cagtttgaga atatgaacct gctggatcag gtggagttgc ttgcacagag
      601 ctacagtctg ctggatcatt tagatgactt tgatgatgat gatgaagacg atgactttga
      661 tccagaacct gaccaggatg agctccctga gtacagtgac gatgatgacc tggagcttca
      721 gggtgctgca gcagccccta tcccaaactt tttctccgat gatgactgcc ttgaagacct
      781 tcctgagaag ttcgatggca accctgacat gctgggtcct ttcatgtatc agtgccagct
      841 cttcatggaa aagagcacca gagatttctc agttgaccgc atccgtgtgt gcttcgtgac
      901 aagcatgctg atcggccgtg ccgcccgctg ggctactgcc aagctgcaaa gatgtactta
      961 cctgatgcac aactacactg cctttatgat ggagctgaag catgtctttg aagaccctca
     1021 gagacgtgaa gctgccaaac gcaagatcag acgtctgcgc cagggccctg ggcctgttgt
     1081 ggactactcc aatgcattcc agatgattgc ccaggacctg gattggactg agcctgccct
     1141 gatggatcag ttccaggaag gtctcaaccc agacattcgc gcagagctgt ctcgccagga
     1201 ggcccccaag accctggctg ctctgattac tgcctgtatt cacatcgaga gaaggctggc
     1261 tcgtgacgct gctgcaaagc ccgatccttc acccagagcc ttggtgatgc ctccaaacag
     1321 ccagaccgat cccaccgagc ctgtgggagg tgcccgcatg cgcctgtcca aggaagaaaa
     1381 ggagagacgc cgcaaaatga atttgtgtct ctactgtggc aatggaggcc atttcgccga
     1441 cacgtgtcca gcgaaagcct ccaagaattc gccgccggga aactccccgg ccccgctgta
     1501 gggggacctt cagcgacagg gccagaacga ataaggtccc caccctccga ggcttcgact
     1561 cagcacctgc aagtgatgct ccagattcat atgccgggca gacccaccct gtttgtccga
     1621 gctatgattg attctggtgc atctggcaac ttcattgatc aagactttgt catacaaaat
     1681 gcaattcctc tcagaatcaa agactggcca gtgatggtgg aagctattga tgggcatcca
     1741 attgcctcgg gcccaatcat tttggaaacc caccacctga tagttgatct gggagaccac
     1801 cgtgagatac tgtcatttga tgtgactcag tctccattct ttcctattgt cctaggaatt
     1861 cgttggctga gcacgcatga ccctcacatt acctggagta cccgctccat tgtcttcaac
     1921 tctgattact gccgacttcg ctgccggatg tttgcacaga taccttctaa cttactgttt
     1981 acagtgccac aaccgaattt gcatccgtat ctacttcatc atgtgcatcc gcatgtccat
     2041 ccgcatatgc atcagcatct gcatcagcat ctgcatcagt ttctgcatcc agatccgcat
     2101 cagtatccgc atccggatcc gcattatcat catcatcagc aggcggatat gcagcaccaa
     2161 ctgcagcagt atctatatca gtatttgtat taccatctgt atccggttat gcaccaccat
     2221 ctgcctccag atcagcatga gcatctgcat gagtatctgc atcagtatct gcatcagtat
     2281 ctgcatcagt ttctgcatca ccatctgcat ccggatctgc atcagtatct gtatcagtat
     2341 ctgcataacc atatgaatcc ggatccacat caccatcctc atccagatcc ccctcaggat
     2401 ccacatcacc ctccacatca ggatccacat cagcatccgg atccccatca ggatcctcca
     2461 catcaggatc cacatcagca tccggatccc catcaggatc ctccacatca ggatccacat
     2521 cagcatccgg atccccatca ggatcctcca catcaggatc cacatcagga tgcacatcag
     2581 catcaggatc cccatcagga tgcacatcag gatccacatc aggatgcaca tcaggatccc
     2641 catatggatc cacacctgca tcagcaccag catccgcagc cgcagccgca tccacaacag
     2701 catcctaacc atcctcagca gccaccattc ttctaccaca tggctggatt cagaatttac
     2761 caccctgtaa ggtattacta tattcagaat gtgtatacac ctgttgatga gcatgtctat
     2821 ccgggtcacc gggtggttga ccctaacatt gagatgattc ctggagcgca cagcctgccc
     2881 agtggacatt tgtactcaat gtctgagtct gaaatgaatg ctctgcgaaa tttcgtggac
     2941 aggaatgtta aagatgggct catgactccc actgtggcgc ccaatggagc ccaagtcctg
     3001 caagtgaaaa gagggtggaa actccaagtc acttacaatt gccgagctcc acagagtggc
     3061 accatccaaa atcagtacct acgcatgtct cttccaaata tgggagaccc tgcacacctg
     3121 gcaagctatg gtgaatttgt ccaagttcct ggctacccat atccagccta tgtttactat
     3181 acaagcccgc atatgatgac tgcgtggtac ccagtaggac gagatgtaca tggacgaata
     3241 atcgttgtgc ctgttgtaat cacctggtct caaaatacga accgccagcc tccggtgccc
     3301 cagtatcctc ctccgcagcc acctccacca ccaccaccac ctccaccgcc accaccacct
     3361 ccaccagcat catcctgcag tgctgcgtag aacctgtcat gtcctttgta gtctctgccc
     3421 tcaacttgat cctgtgcagc ttctcaatct atgactgtgt ggtactggac cttcagaggc
     3481 gcacagagct caagtcagtt ttcgtcttga ctgccacttt ataagttgac aggcctgggt
     3541 tttacttgtt aaaacctctc accatctcaa tcacaggctg ccaagtgtct ttacaaagaa
     3601 gctgatacaa acacaggcca tgctgatttc ttacagaggg agagaagagg aagagaagaa
     3661 gaaagaggag gaagaggaca tgacttgccc atatgctggg caccttataa aggaagccag
     3721 acttttcggt gcagtatgga aaggcttccg tgattctctt gctgcacccc acgaaacttc
     3781 accaccttca aactccattt tcacggttcc gttaattttc aaggagcagc aactcgactg
     3841 gttctctgct acatgaaaca cctcagcttg aaaaggaagt gctctctcag actgacttgt
     3901 gagtgtgcct tcacattctg gtgcaaatca tgtgtaccca agaactctga catagcatct
     3961 taccatcatc atgccaggaa ttggtttcct taaagtgtga cacttggcaa ccagaagcgt
     4021 ctggtggctt caggtgtgtt agggttttgt agaaagttgt aaccacctct cagtcatgca
     4081 agagggtaca tcaaaaagac ttctaaattt ggtacccaga ctttgacaag agcctgtgag
     4141 ttagttcatt gtaaccaact tgtcaggaac ttcagcaaga attttcatga atgttgttaa
     4201 ttggctggag aaataccagc aaagttttgc aatcatccac ccatttgtct gccattgtgc
     4261 atctggacat ccatcaaccg cccactcagt ggcatctacc atgaagatgt ttagagacgg
     4321 gaaatgattt tgcacccaga tccacactcc tctcatgtca gatatttgga attagagcac
     4381 cgggaatcgt cttgaagata tgaacttcta agaatggagt ctcttgctac taccttgaaa
     4441 catttgttta tcctctcttt ctgtttgatt ctactactac cattaaccct gctgcaggat
     4501 ttgtcaccat tctgcctgac tgctgagact ccattttgct gctatgcaag gagatgaaag
     4561 aggcaggcct aaaggagcag taggacataa catggttttt atttctatct gtcatgatct
     4621 taatggtgaa gtttcctttt tcgtcagcat ttatctcttt gttatcctga catgttttaa
     4681 ttagtttagt gggttttttt tttctattgg tggtggtgtt cttttgttgt tgttttttgt
     4741 ggtcgtcatt ttgattttgg atcacttcgt gttttacagt aattactttt aaatggtgca
     4801 tttgcttctg attttttttt tttatgaagc atcacatcag tttacctcat atctcaattc
     4861 catccttcat gcattttttt tttaactcat ttgatcttca agctgcagag ggcctagcaa
     4921 tgggtcatca cctgcagccc tggcatgtac acacggacat ttgccaccac tgaaagcaaa
     4981 cagttggaga agttggccac ctgagtcaag gaggtggtgc tggtgtgagt tcacagctca
     5041 caggggacgg tgaacgttga cattgacttt ggcagtgtgt actatgctct actcctatat
     5101 atactctata gatgttaggc attaaggata agtgatctta aatttactga aattttgtta
     5161 agttgattag atttacatta attgtttata ttgtgttttt tcttttattt tagcacttcc
     5221 aacaaaaatg cctttattca tttattagga agaaattgga gtggcgaaca caaactagca
     5281 aaattaatta attggctgtg ggcccaaatt tgaattcctt attaattcaa agtgaaggaa
     5341 gtgtgagagt ttctattgct ggtcatgaag ttcaccacga agagtcagtt tagtttgtac
     5401 cgagaggcat ttagctgaga gtgatttgag ttgggcatct ctatgatgca gtccagactt
     5461 gtgtttaagt ttacaggtac ctcttggact cctgaattga tctcgatgtc acacatcatc
     5521 gacatcccgc atcccacaca gttccaagat tggcgacaga gagcatcctg ttggagagac
     5581 ctgaccagtc acgaaggacc gcctgcagac tcttctgcag gaccatgcta catattcagc
     5641 tggagaggga acacgcatct gatgggaatg gattgttttt gcttgtttca gaattttgct
     5701 gttgagatgt tgttaaactt tgcattgttt ttcttttttc tttttttttc cctatagtca
     5761 attaagaata aggggtagat aatcataagt attttgggct gggagggatt gttaagtaat
     5821 cttaggtggg tgggtaattt aggaaattaa gttaggataa gataggataa gataagataa
     5881 gctaggataa gttagataaa ttaggataag ttagataagt taggataaat tccataagat
     5941 aggataagtt ccataagata ggataagtta gttcacatag gataagcata ggataagtta
     6001 gatcacatag gataagttag atcacatagg ataagttaga tcaaatacct caacaagtgg
     6061 acgagtgtac ttattggtcc cttaccccta ccaactttac ctttaaggcc gagctcagag
     6121 ggaactacag ggaaatcagt gttaggagtg aattggacat ggatgacatt ctagtgaagg
     6181 ttaggacgat tagagtaatc taatacaggc tctgggaatt aactcaaggg attttaggaa
     6241 tgccagatga cagtcatcat ttgataaaac tgttgctgga ataaagttta gaatgttgaa
     6301 tggtaaatgt taatggaaat tgtacgaaaa tgaataaaag ctctttccat acccctcccc
     6361 ctccctaccc cactctttct ttcctccccc cctccataac ctaccccttc tcccaaacac
     6421 acccctccat ttcctaactc accacgtctc cccttccact ccctaaccca ccccttcttc
     6481 cctccctccc tccagaaccc gcccccatct tccccctccc taacacaccc cttcttcctt
     6541 tccccctcca accccaccct tcaaactata ttactgtatt ctctttatta tatttctttg
     6601 tattaataaa actgtattga tgttacaata aaaattcaat aaataaagac ttagtggtta
     6661 aggcaaaaaa aaaaaaa
//
