LOCUS       NM_001081321            8999 bp    mRNA    linear   ROD 09-JUN-2024
DEFINITION  Mus musculus PDS5 cohesin associated factor A (Pds5a), mRNA.
ACCESSION   NM_001081321 XM_006504117 XM_893051
VERSION     NM_001081321.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Bsteh,D., Moussa,H.F., Michlits,G., Yelagandula,R., Wang,J.,
            Elling,U. and Bell,O.
  TITLE     Loss of cohesin regulator PDS5A reveals repressive role of Polycomb
            loops
  JOURNAL   Nat Commun 14 (1), 8160 (2023)
   PUBMED   38071364
  REMARK    GeneRIF: Loss of cohesin regulator PDS5A reveals repressive role of
            Polycomb loops.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Arruda,N.L., Bryan,A.F. and Dowen,J.M.
  TITLE     PDS5A and PDS5B differentially affect gene expression without
            altering cohesin localization across the genome
  JOURNAL   Epigenetics Chromatin 15 (1), 30 (2022)
   PUBMED   35986423
  REMARK    GeneRIF: PDS5A and PDS5B differentially affect gene expression
            without altering cohesin localization across the genome.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8999)
  AUTHORS   Viera,A., Berenguer,I., Ruiz-Torres,M., Gomez,R., Guajardo,A.,
            Barbero,J.L., Losada,A. and Suja,J.A.
  TITLE     PDS5 proteins regulate the length of axial elements and telomere
            integrity during male mouse meiosis
  JOURNAL   EMBO Rep 21 (6), e49273 (2020)
   PUBMED   32285610
  REMARK    GeneRIF: PDS5 proteins regulate the length of axial elements and
            telomere integrity during male mouse meiosis.
REFERENCE   4  (bases 1 to 8999)
  AUTHORS   Morales,C., Ruiz-Torres,M., Rodriguez-Acebes,S., Lafarga,V.,
            Rodriguez-Corsino,M., Megias,D., Cisneros,D.A., Peters,J.M.,
            Mendez,J. and Losada,A.
  TITLE     PDS5 proteins are required for proper cohesin dynamics and
            participate in replication fork protection
  JOURNAL   J Biol Chem 295 (1), 146-157 (2020)
   PUBMED   31757807
REFERENCE   5  (bases 1 to 8999)
  AUTHORS   Carretero,M., Ruiz-Torres,M., Rodriguez-Corsino,M., Barthelemy,I.
            and Losada,A.
  TITLE     Pds5B is required for cohesion establishment and Aurora B
            accumulation at centromeres
  JOURNAL   EMBO J 32 (22), 2938-2949 (2013)
   PUBMED   24141881
REFERENCE   6  (bases 1 to 8999)
  AUTHORS   Zhang,B., Chang,J., Fu,M., Huang,J., Kashyap,R., Salavaggione,E.,
            Jain,S., Kulkarni,S., Deardorff,M.A., Uzielli,M.L., Dorsett,D.,
            Beebe,D.C., Jay,P.Y., Heuckeroth,R.O., Krantz,I. and Milbrandt,J.
  TITLE     Dosage effects of cohesin regulatory factor PDS5 on mammalian
            development: implications for cohesinopathies
  JOURNAL   PLoS One 4 (5), e5232 (2009)
   PUBMED   19412548
  REMARK    GeneRIF: While Pds5A(-/-) and Pds5B(-/-) mice die at birth, embryos
            harboring 3 mutant Pds5 alleles die between E11.5 and E12.5 most
            likely of heart failure, indicating that total Pds5 gene dosage is
            critical for normal development.
            Erratum:[PLoS One. 2009;4(5). doi:
            10.1371/annotation/ea5b7eb5-5087-448a-8325-c8efff1f54d9.
            Shashikant, Kulkarni [corrected to Kulkarni, Shashikant]]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC112263.4.
            
            On Jun 19, 2019 this sequence version replaced NM_001081321.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.5826.1,
                                           SRR10223970.7494.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-487               AC112263.4         171808-172294       c
            488-662             AC112263.4         170821-170995       c
            663-866             AC112263.4         140166-140369       c
            867-953             AC112263.4         138486-138572       c
            954-1051            AC112263.4         137914-138011       c
            1052-1178           AC112263.4         133665-133791       c
            1179-1259           AC112263.4         132405-132485       c
            1260-1400           AC112263.4         130326-130466       c
            1401-1516           AC112263.4         128847-128962       c
            1517-1611           AC112263.4         128063-128157       c
            1612-1757           AC112263.4         126446-126591       c
            1758-1909           AC112263.4         125203-125354       c
            1910-2023           AC112263.4         124451-124564       c
            2024-2105           AC112263.4         121982-122063       c
            2106-2154           AC112263.4         121146-121194       c
            2155-2294           AC112263.4         120391-120530       c
            2295-2410           AC112263.4         118078-118193       c
            2411-2516           AC112263.4         112615-112720       c
            2517-2677           AC112263.4         111800-111960       c
            2678-2801           AC112263.4         109373-109496       c
            2802-2960           AC112263.4         108230-108388       c
            2961-3029           AC112263.4         105771-105839       c
            3030-3166           AC112263.4         103977-104113       c
            3167-3290           AC112263.4         102057-102180       c
            3291-3495           AC112263.4         101618-101822       c
            3496-3610           AC112263.4         101143-101257       c
            3611-3743           AC112263.4         97978-98110         c
            3744-3863           AC112263.4         97038-97157         c
            3864-3926           AC112263.4         94608-94670         c
            3927-4072           AC112263.4         93678-93823         c
            4073-4181           AC112263.4         93002-93110         c
            4182-4522           AC112263.4         89322-89662         c
            4523-8999           AC112263.4         79777-84253         c
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5"
     gene            1..8999
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /note="PDS5 cohesin associated factor A"
                     /db_xref="GeneID:71521"
                     /db_xref="MGI:MGI:1918771"
     exon            1..487
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            488..662
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             528..4526
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /note="PDS5, regulator of cohesion maintenance, homolog A"
                     /codon_start=1
                     /product="sister chromatid cohesion protein PDS5 homolog
                     A"
                     /protein_id="NP_001074790.1"
                     /db_xref="GeneID:71521"
                     /db_xref="MGI:MGI:1918771"
                     /translation="MDFTQPKPATALCGVVSADGKIAYPPGVKEITDKITTDEMIKRL
                     KMVVKTFMDMDQDSEDEKQQYLPLALHLASEFFLRNPNKDVRLLVACCLADIFRIYAP
                     EAPYTSHDKLKDIFLFITRQLKGLEDTKSPQFNRYFYLLENLAWVKSYNICFELEDCN
                     EIFIQLFRTLFSVINNSHNTKVQMHMLDLMSSIIMEGDGVTQELLDSILINLIPAHKN
                     LNKQSFDLAKVLLKRTVQTIEACIANFFNQVLVLGRSSVSDLSEHVFDLIQELFAIDP
                     QLLLSVMPQLEFKLKSNDGEERLAVVRLLAKLFGSKDSDLATQNRPLWQCFLGRFNDI
                     HVPVRLESVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVTIITAAKRDLA
                     LVNDQLLGFVRERTLDKRWRVRKEAMMGLAQLYKKYCLHGEAGKEAAEKVSWIKDKLL
                     HIYYQNSIDDKLLVEKIFAQYLVPHNLETEERMKCLYYLYASLDPNAVKALNEMWKCQ
                     NMLRSHVRELLDLHKQPTSEANCSAMFGKLMTIAKNLPDPGKAQDFVKKFNQVLGDDE
                     KLRSQLELLISPTCSCKQADVCVREIARKLANPKQPTNPFLEMVKFLLERIAPVHIDS
                     EAISALVKLMNKSIEGTADDEEEGVSPDSAIRSGLELLKVLSFTHPTSFHSAETYESL
                     LQCLRMEDDKVAEAAIQIFRNTGHKIETDLPQIRSTLIPILHQKAKRGTPHQAKQAVH
                     CIHAIFSNKEVQLAQIFEPLSRSLNADVPEQLITPLVSLGHISMLAPDQFASPMKSVV
                     ANFIVKDLLMNDRSTGEKNGKLWSPDEEVSPEVLAKVQAIKLLVRWLLGMKNNQSKSA
                     NSTLRLLSAMLVSEGDLTEQKRISKSDMSRLRLAAGSAIMKLAQEPCYHEIITPEQFQ
                     LCALVINDECYQVRQIFAQKLHKALVKLLLPLEYMAIFALCAKDPVKERRAHARQCLL
                     KNISIRREYIKQNPMATEKLLSLLPEYVVPYMIHLLAHDPDFTRSQDVDQLRDIKECL
                     WFMLEVLMTKNENNSHAFMKKMAENIKLTRDAQSPDESKTNEKLYTVCDVALCVINSK
                     SALCNADSPKDPVLPMKFFTQPEKDFCNDKSYISEETRVLLLTGKPKPTGVLGTVNKP
                     LSATGRKPYVRSAGTETGSNINANSELSPSAGSRSREQSSEASETGVSENEENPVRII
                     SVTPVKNIDTVKNKEINSDQSTQGNISSDRGKKRIVTAAGAENIQKPDEKVDESGPPA
                     PSKPRRGRRPKSESQGNATKNDDLNKPVSKGRKRAAGSQESLEAGNAKAPKLQDGAKK
                     AVPAERQIDLQR"
     exon            663..866
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            867..953
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1051
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1178
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1259
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1400
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1516
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1611
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1757
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1909
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2023
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2024..2105
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2154
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2155..2294
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2295..2410
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2411..2516
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..2677
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..2801
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..2960
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2961..3029
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3030..3166
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3290
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3291..3495
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..3610
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3611..3743
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3744..3863
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3864..3926
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3927..4072
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4073..4181
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4182..4522
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            4523..8999
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5649..5654
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /note="hexamer: AATAAA"
     polyA_site      5671
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /note="major polyA site"
     regulatory      6917..6922
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /note="hexamer: AATAAA"
     polyA_site      6945
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
     regulatory      8972..8977
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      8999
                     /gene="Pds5a"
                     /gene_synonym="9030416H16Rik; B230308N11Rik;
                     E230024D05Rik"
ORIGIN      
        1 gctctcgcgg gctctcgcgc cgctcgcgtg acgggctccg gctgtgcgcg gcgacgctca
       61 ggctccgcgg ggcacgggcg ggccgggagc gcgccgcggc gagagggcgt ctggtgcggg
      121 tgggcgcgag gtgcgggcgg ccggccggcg agcgagcgag cggccgggcg ggcgcggggg
      181 acgcgcgggc gtgcacccgc gcgcgggctc tcggaggagc cgcggccgag gctgcgcttc
      241 ctcagcccga cggagagcgg cagtgtcgcc ccggccggcg cacgctcggc ccgcttctcc
      301 ccccgcggcg gctgctcctc ggcaccgcca gccccagcgc cgctccgggg cgggcgggcg
      361 gcggcggcgg cggcggcggg acggacggag ccgctttgtg tgcagcccga cgaggggcgg
      421 cggcggcggc gcaaccacct gacagaggcc cggcgctcgc tgcaccgtcc gcccgcatga
      481 ggaggagagg ccggtagagg actgtgagag aaaagttatt ccccaggatg gacttcacgc
      541 agccgaagcc tgccactgcc ctctgtggcg tcgtgagtgc ggacgggaag atcgcttacc
      601 ctccgggagt aaaagagatc accgacaaga tcaccaccga tgaaatgatc aaacgtctga
      661 agatggtagt gaaaactttt atggatatgg atcaggattc agaagatgaa aagcaacagt
      721 atctcccact agccttgcat ctcgcatctg aattctttct caggaaccct aataaagatg
      781 tgcgccttct cgtagcatgt tgtttggctg atatatttcg aatctatgcc ccagaagctc
      841 catatacttc ccatgataaa cttaaggaca tatttctctt tattaccaga cagttaaaag
      901 gtttggagga tacaaagagt ccacagttta atagatattt ttatttatta gagaatttag
      961 cttgggttaa atcatataac atctgctttg agttggaaga ttgcaatgaa atttttattc
     1021 agctttttag aactctcttc tcggtaatca acaatagcca caatacgaaa gtacaaatgc
     1081 acatgttaga cttgatgagt tctatcatca tggaaggtga tggagttact caagaattat
     1141 tggattccat tcttatcaac ctcatccctg cacacaagaa cttaaataaa cagtccttcg
     1201 accttgcaaa agtcctactg aaaaggacag tccagactat tgaagcatgt attgccaatt
     1261 ttttcaatca agtcctggtg ctgggcagat catcagtcag cgacctgtct gaacacgtat
     1321 ttgatctgat tcaggaactt tttgctatcg atcctcagtt actgttatct gtcatgccac
     1381 agcttgaatt caaactgaag agcaacgatg gtgaagaacg cctagctgtg gttcgactcc
     1441 tcgcaaaatt gttcggctct aaagattcag atttagcaac acagaatcgg cctctctggc
     1501 agtgctttct tgggcgattt aatgacattc atgttcctgt gaggttagaa agtgtgaagt
     1561 ttgccagcca ctgtttgatg aatcacccag acttagcgaa ggatctgaca gaatatttga
     1621 aagttaggtc acatgatcca gaagaagcca ttcgtcatga tgttattgtt actataataa
     1681 cagctgccaa aagagacctt gccttagtaa atgatcagtt gcttggcttt gtcagggaaa
     1741 ggacactgga taaacggtgg cgagtaagaa aagaagccat gatgggtctg gctcagctct
     1801 ataagaaata ctgtcttcat ggggaagcag gaaaggaagc cgcggagaaa gtcagctgga
     1861 taaaggacaa gctgctgcat atctactatc agaacagcat tgatgacaaa ctattggtag
     1921 agaaaatctt tgctcagtat cttgttcccc acaacctgga aacagaagag agaatgaaat
     1981 gcttatatta tttatatgct agcttggatc caaatgctgt aaaagctctc aatgaaatgt
     2041 ggaaatgtca gaacatgctt cgaagtcatg tgcgtgaact attggactta cacaagcagc
     2101 ctacatcaga agcgaactgt tctgccatgt ttgggaaact gatgaccata gcaaagaatt
     2161 tgcctgaccc tggaaaagca caagattttg taaagaaatt taaccaggtt cttggtgatg
     2221 atgagaaact gaggtctcag ttggaattat taatcagccc aacctgttca tgcaagcagg
     2281 ctgacgtttg tgtgagggaa atagctcgga aacttgcaaa tcctaagcag ccaaccaacc
     2341 cttttctaga gatggtcaaa tttcttttgg aaagaattgc tcctgtgcac atcgattcag
     2401 aagccataag tgcactggta aaactgatga ataagtcaat cgaagggacg gccgatgatg
     2461 aagaggaggg tgtcagtcca gactcagcca tccgctcagg acttgagctt cttaaggttc
     2521 tgtctttcac acatcctacc tcgttccact ctgcagagac atatgagtcc ttgttacagt
     2581 gcctaagaat ggaggatgac aaggtagcag aagctgcaat acaaattttt agaaacacag
     2641 gccacaaaat agaaactgac cttccccaga tacggtcaac cttgatcccc attttacatc
     2701 agaaagccaa gcggggcact ccacaccaag caaagcaggc tgttcactgc atccatgcca
     2761 tcttctcaaa caaggaggtc cagctggcac agatttttga gccactcagt aggagtctga
     2821 atgctgatgt accagagcaa cttataactc cattagtttc actgggccac atttccatgt
     2881 tagcaccaga tcagtttgcc tccccgatga aatctgtagt ggcaaacttt attgttaaag
     2941 accttctaat gaacgacagg tcaacaggtg agaagaatgg aaaattatgg tctccagatg
     3001 aggaagtttc tcctgaagtt ctagcaaagg tacaggcaat taaacttctg gtaaggtggc
     3061 tgttgggtat gaaaaacaac cagtctaaat ctgccaactc aactcttcgg ttattatcag
     3121 ccatgctggt tagtgagggc gacctgacag agcaaaagag gatcagtaaa tctgatatgt
     3181 ctcgcttgcg attagctgct ggtagtgcca taatgaagct tgctcaggaa ccttgctacc
     3241 atgaaattat taccccagaa cagtttcagc tctgtgcact ggttattaat gatgagtgct
     3301 accaagtaag gcagatattt gcccagaagc ttcataaggc acttgtgaag ttgcttctcc
     3361 cactggagta tatggccatc tttgctttgt gtgccaaaga ccctgtgaaa gaaaggagag
     3421 cacacgctcg gcagtgtttg ttaaagaaca tcagcatccg cagggagtac atcaaacaga
     3481 accccatggc cactgagaaa ttattgtcac tgctgcctga atatgtggtt ccatacatga
     3541 ttcacctcct agcccatgat cctgatttca cacggtcaca agatgttgat caacttcgtg
     3601 atataaaaga gtgcctgtgg tttatgcttg aagtcttaat gacaaagaat gaaaacaaca
     3661 gccatgcatt catgaaaaag atggcagaga atatcaagct aaccagagac gcccagtctc
     3721 ccgatgaatc caagacaaat gaaaaacttt atacggtttg tgacgtggct ctgtgtgtta
     3781 taaatagtaa aagtgctttg tgcaatgcag actcaccaaa ggacccagtc ctcccaatga
     3841 agttctttac acagcctgaa aaggacttct gtaatgacaa aagctatatt tcagaagaga
     3901 caagagttct tctgttaaca ggaaagccaa agcctactgg agtattaggt acagtgaaca
     3961 agcctttatc agcaacggga aggaagcctt atgttagaag cgccggcaca gagactggaa
     4021 gcaatattaa cgccaattca gagctgagtc cttcagccgg aagtcgttca agggaacaga
     4081 gttcagaggc atcagaaact ggagttagtg aaaatgagga gaatcctgtg agaataattt
     4141 ctgtcacacc tgtaaagaat attgatactg taaagaataa ggaaattaat tctgatcagt
     4201 ctacccaagg caacatcagc agtgacagag gaaagaaaag aattgtaaca gcagctggtg
     4261 cagagaatat ccaaaagcca gacgagaaag tagatgagtc aggaccgcct gccccttcca
     4321 aaccccggag aggacgtcgc cccaaatctg aatctcaggg caatgcaacc aaaaacgatg
     4381 atctaaataa acctgttagc aagggaagga aaagagctgc aggcagccag gagagtctgg
     4441 aggcagggaa tgccaaagcg cccaagctac aagatggagc caaaaaggca gttccagcag
     4501 agagacaaat tgatttacaa aggtaaaagg agaaaacgaa ggccaaacag aagcagagtc
     4561 caggttctgt aaaaacttgg attccaatgt ccccaaacag aaaacgaagc tcacttcaaa
     4621 tacacacttt ctgccttgaa aactgaacga agctagcctt cctttttaca cagcctaagt
     4681 cctctggtga aatgtacagc agactctaga gaggctaaga gcatctctgt tcttcccctc
     4741 ccccagactt ttcctgggaa aagtcaataa ttaagcaaat tgcttaacac ttggttccag
     4801 gtcctgcata tctggaactt aaaggcatag taaccatttt ttccatgctg cagatttcat
     4861 tttagagagg aacagggcgt ccattcactg atgctgagaa ctggtccatc ctagagccca
     4921 gaaccccgtg ccacacaggc gagcaggtgt ctactgaact gaggctttac aagcgagaga
     4981 gcagatgaca tatttcttcg ttttcctttt ggaaacttat gtattattct ttctgcctgt
     5041 ctacttttct gcaaaaaatg agatgtacag atttctgttc cctgctatga aaagtaatgt
     5101 ggcaatttta taaatgttgc tttctgattt ttatcagaat gagaaaataa ttaaaattat
     5161 tgatctgcaa gtagtaaaca cttcatatta tgatttctcc cattttagtt taatataatt
     5221 tgcaataaat gtacatattg tttcataagg catatcagtt tagaatggtt tttactcctg
     5281 tgattatgat ggaataattg ggttttcagg gagtaaagac tgtccaggat ttggttttat
     5341 aattgtcacc agatttttat tattaatgta aaaaaaggtt ggttttgttt ttagggtttg
     5401 ttttgttttt ttggaactat cagtagatgg acactggcag ctttgtgagg gacagagata
     5461 tgctctcgac gtctggcacc gtgctgttgg gaaataggcc ttttattttt gtctgataat
     5521 tggggctcag tttttatttt tattttacag acaactgtta caatattgta ttccttggca
     5581 ttcattgttc agcatagata ctgtgtacag tatttctgtg cacataatcc tatttaattt
     5641 ttgcataaaa taaatgcttt tagatgtctt atgtaagtct tatcttttta tcatgctttg
     5701 ctttcttagg aatttttatg tgaaagggct aaagtcacag aaggacatga ctacagccaa
     5761 ctctccctta tctatgtaaa atagtgactg tgcctcaggt gctcggtttg taggactatg
     5821 gacatgtgca agaatgagga gacgtttgaa attaattttc tcttatcctc ctcaggtgtt
     5881 ttgcctgtct ccactgtttc tgcttggctt gtacccaaaa tcctagtggg acgagctgct
     5941 ctgtttgcat tatgacttgg tcttgctggg atgtgtaaaa tcactgaagt atcaaaactc
     6001 tccagaatga aattctttga tagtatgtca attaggttct catagataag tgagagttgg
     6061 ctataaattg attctgggtg aaaggtgggc ttcttttaag tattttacat gtacatgttt
     6121 aggtgtcaga agatggctag agaacagtga ataccaatga tagaaacaat atttcttgaa
     6181 agggcgtaaa agccctgctc ttcctgttgc ctccaatctg ctgacagagc atctatgcat
     6241 accgtcagtc aggtccctca ttcagtcagt gctatattct atttacaagt ttgacttagg
     6301 gccaccagtt tgtcactacc aaaacttctt tgctggaacc cagtgaactc tatgttgagc
     6361 agttagtcac acagtgtaag gaagaaggtt cctaaatgtt aagggcactg gtcagaatac
     6421 agcagaagcg ttcacatctg ttacagctct ttggagtttt gcattgaaac agttactgtc
     6481 agggtctgtt cctcatgagt gcctggtctc tggcaccaag agtaaactgg ttggggatta
     6541 agcaaattga gagggacttg tttactggaa atgtgtcagg ttttctttga aaaagaaaat
     6601 gttaaaatct cttaggaatt tggtatttac atctcagaga aatacaacac aaaagtgcag
     6661 acttaaaatt gagaattaat gttaaccctt tgtgtctagt ttgaagcttc ttgtatttgt
     6721 ctaaaactac aagccagaat tttgtatctc ctttgataaa aagtgtgtat actgtaaagt
     6781 agttttgcat attcttgtgc tgcacatggg ctggattttt agaatttttt taaagacttt
     6841 aagccgaacc ttgtaatttg tgtaaatgac aagtgtaaaa tccttccata aaatgcttaa
     6901 aatatgcact gtttcaaata aaaccaagaa atgcagcatt atgtagaagt gtggaaacgt
     6961 gaatattcat gtgcccatgt gaagggctga tgaagggacc atcgtgcact ttccattttt
     7021 caagcctctg atgtagctca cagtgacagt acagggggat caattctgat ggatctttgc
     7081 caaagtttct taaagatgat cattgtttcc tactattctc tgtgtcacaa atggtggaca
     7141 ttatatacta ggttttttgt ttttgttttt tttgagacag ggtttccctg cctcccgagt
     7201 gctgagatta aaggtgtgca ccaccaccgc ctgaccatta tatactagtt taataacttt
     7261 aatcggagtg aaccgatgtc taagattcgg atagaataag aggttaagag aggccatcag
     7321 cctatctatg tctgatgcct cccatagttg gcctcttgat agtctataac gtggctaaaa
     7381 aagactaaag gcaaagaata ttggggaggg gggcttattt gtactgtttt tttttttttc
     7441 attagtctcc agactgcact atatattagc cctttgtttt aataaaaact ctatgatcag
     7501 cgttcccaga atgaagtata aggcagaaca ggtctcagtc ttatgtggtc actgcactct
     7561 ttcatatggg cctagaacat ttacaaaggt aggatatctc aaaggtaggt tgagcatagg
     7621 cctcaaccaa tgtccatagt gagggaccag tgtattgatc agacttctct gtctacattc
     7681 caacaagtct cttaccagct ctgacaactg aacttgctga ggtaatgtag ccctcaggct
     7741 gccaagcctt ctgtcactca ttctctgaca ctgttccttt cctggttgtt gttttattaa
     7801 gatttgcttg aatttatgcc tgttttgatt gcatgtgtac cacatgtatg cctagtgccc
     7861 ttggaggtta gaaaagagtg ttgaataccc tggaagtata gttgtaagcc cccatgtggg
     7921 ggccaggaat tgacccctgg tcctctgcaa aaaataagtg ctcttaatct ttaaactctc
     7981 caacaccccc agcccatgta gttaaagagt tatttaatgc agggtcttat gcataagatg
     8041 tccttgaagg cactgtgtag ttaagggtga acttgaagac ctagtcctcc tgcctctacc
     8101 cctaaagagt tggaactgct agccccacaa gcctttacct tatacttaca ctggaaaaag
     8161 ccagagcttc gagattaaca accaacctat gtgtacacag gcacacactc atgagaataa
     8221 caacctatgt gcacacagga gcacactctg tgtgagaata aacacctgtt tgcttgtctt
     8281 ctgtctggct gcaaattcta gcacaggcag aatgtctaat ttctcaggtt gctggcccaa
     8341 ggtgatgatc aggatttcag gtgggggtac tcttccctta tcaacacttg ttgcctttct
     8401 ccccttggaa cccttttgct cctgacatta cctagcaaag actttattat accaaacaaa
     8461 gtcattctct gtaaacagca ctgtatttat tccttgtttg aaagtggtct atgcaggaag
     8521 ttaaaggcca ctcagttctc tcctattaca aaaaggggag aaaggtagct agctgagagc
     8581 tgataggaat gtaaagtctt ttacagcatc ataagaatat atagcttact ctcaatttct
     8641 ttaaaagcta ttttgtctta atatgattgc tgtatggaag ataagcagta gaagctaaat
     8701 aatacagatg ggcatgagtg tgtgagatcc tatattaagt gcaagaatca cacttaatat
     8761 aggatctcat gatgatgata aaccagccat atactacgag cgacctcaga tcctcctgcc
     8821 cccacacccc aatacctggg attatgggta tatgcagcaa cttgagagct tgtcatgatg
     8881 atggataccg tggctcctga gggccatgtt ttcggtaatg aataatgaaa cctttctaaa
     8941 attgatttgt ggtcgatgtg gaaatctttg cattaaatca tggaattata cacttaaaa
//
