LOCUS       NM_013743               3453 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus pyruvate dehydrogenase kinase, isoenzyme 4 (Pdk4),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_013743
VERSION     NM_013743.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Shigesawa,I., Nakamura,A., Yamauchi,Y., Kawata,S., Miyazaki,A.,
            Nomoto,H., Kameda,H., Terauchi,Y. and Atsumi,T.
  TITLE     Effects of glucokinase haploinsufficiency on the pancreatic
            beta-cell mass and function of long-term high-fat, high-sucrose
            diet-fed mice
  JOURNAL   J Diabetes Investig 15 (12), 1732-1742 (2024)
   PUBMED   39305123
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Khang,A.R., Kim,D.H., Kim,M.J., Oh,C.J., Jeon,J.H., Choi,S.H. and
            Lee,I.K.
  TITLE     Reducing Oxidative Stress and Inflammation by Pyruvate
            Dehydrogenase Kinase 4 Inhibition Is Important in Prevention of
            Renal Ischemia-Reperfusion Injury in Diabetic Mice
  JOURNAL   Diabetes Metab J 48 (3), 405-417 (2024)
   PUBMED   38311057
  REMARK    GeneRIF: Reducing Oxidative Stress and Inflammation by Pyruvate
            Dehydrogenase Kinase 4 Inhibition Is Important in Prevention of
            Renal Ischemia-Reperfusion Injury in Diabetic Mice.
REFERENCE   3  (bases 1 to 3453)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 3453)
  AUTHORS   Cai,W., Wang,X., Deng,Q., Gao,J. and Chen,Y.
  TITLE     Expression and Role of PDK4 on Childhood Dyslipidemia and Lipid
            Metabolism in Hyperlipidemic Mice
  JOURNAL   Horm Metab Res 56 (2), 167-176 (2024)
   PUBMED   38096914
  REMARK    GeneRIF: Expression and Role of PDK4 on Childhood Dyslipidemia and
            Lipid Metabolism in Hyperlipidemic Mice.
REFERENCE   5  (bases 1 to 3453)
  AUTHORS   Nobs,S.P., Kolodziejczyk,A.A., Adler,L., Horesh,N.,
            Botscharnikow,C., Herzog,E., Mohapatra,G., Hejndorf,S.,
            Hodgetts,R.J., Spivak,I., Schorr,L., Fluhr,L., Kviatcovsky,D.,
            Zacharia,A., Njuki,S., Barasch,D., Stettner,N., Dori-Bachash,M.,
            Harmelin,A., Brandis,A., Mehlman,T., Erez,A., He,Y., Ferrini,S.,
            Puschhof,J., Shapiro,H., Kopf,M., Moussaieff,A., Abdeen,S.K. and
            Elinav,E.
  TITLE     Lung dendritic-cell metabolism underlies susceptibility to viral
            infection in diabetes
  JOURNAL   Nature 624 (7992), 645-652 (2023)
   PUBMED   38093014
REFERENCE   6  (bases 1 to 3453)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 3453)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   8  (bases 1 to 3453)
  AUTHORS   Holness,M.J., Bulmer,K., Gibbons,G.F. and Sugden,M.C.
  TITLE     Up-regulation of pyruvate dehydrogenase kinase isoform 4 (PDK4)
            protein expression in oxidative skeletal muscle does not require
            the obligatory participation of peroxisome-proliferator-activated
            receptor alpha (PPARalpha)
  JOURNAL   Biochem J 366 (Pt 3), 839-846 (2002)
   PUBMED   12099888
  REMARK    GeneRIF: upregulation of PDK4 in skeletal muscle
REFERENCE   9  (bases 1 to 3453)
  AUTHORS   Wu,P., Peters,J.M. and Harris,R.A.
  TITLE     Adaptive increase in pyruvate dehydrogenase kinase 4 during
            starvation is mediated by peroxisome proliferator-activated
            receptor alpha
  JOURNAL   Biochem Biophys Res Commun 287 (2), 391-396 (2001)
   PUBMED   11554740
  REMARK    GeneRIF: The activation of pyruvate dehydrogenase kinase 4 during
            starvation is mediated by peroxisome proliferator-activated
            receptor alpha
REFERENCE   10 (bases 1 to 3453)
  AUTHORS   Bowker-Kinley,M. and Popov,K.M.
  TITLE     Evidence that pyruvate dehydrogenase kinase belongs to the
            ATPase/kinase superfamily
  JOURNAL   Biochem J 344 Pt 1 (Pt 1), 47-53 (1999)
   PUBMED   10548532
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC023174.4.
            
            On Nov 17, 2006 this sequence version replaced NM_013743.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ001418.1, AK004543.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-246               AC023174.4         70909-71154         c
            247-388             AC023174.4         69766-69907         c
            389-460             AC023174.4         67582-67653         c
            461-645             AC023174.4         66706-66890         c
            646-732             AC023174.4         66467-66553         c
            733-810             AC023174.4         65946-66023         c
            811-887             AC023174.4         64011-64087         c
            888-986             AC023174.4         62255-62353         c
            987-1097            AC023174.4         61940-62050         c
            1098-1211           AC023174.4         61554-61667         c
            1212-3453           AC023174.4         58227-60468         c
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 2.06 cM"
     gene            1..3453
                     /gene="Pdk4"
                     /note="pyruvate dehydrogenase kinase, isoenzyme 4"
                     /db_xref="GeneID:27273"
                     /db_xref="MGI:MGI:1351481"
     exon            1..246
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..1355
                     /gene="Pdk4"
                     /EC_number="2.7.11.2"
                     /note="[Pyruvate dehydrogenase [lipoamide]] kinase isozyme
                     4, mitochondrial; pyruvate dehydrogenase kinase 4"
                     /codon_start=1
                     /product="[Pyruvate dehydrogenase (acetyl-transferring)]
                     kinase isozyme 4, mitochondrial"
                     /protein_id="NP_038771.1"
                     /db_xref="CCDS:CCDS19902.1"
                     /db_xref="GeneID:27273"
                     /db_xref="MGI:MGI:1351481"
                     /translation="MKAARFVMRSASSLSSASLVPREVELFSRYSPSPLSMKQLLDFG
                     SENACERTSFAFLRQELPVRLANILKEIDILPDRLVNTPSVQLVKSWYIQSLMDLVEF
                     HEKSPEDQKALSEFVDTLVKVRNRHHNVVPTMAQGILEYKDTCTVDPVTNQNLQYFLD
                     RFYMNRISTRMLMNQHILIFSDSKTGNPSHIGSIDPNCDVVAVVQDAFECAKMLCDQY
                     YLTSPELNLTQVNGKFPGQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENRPSLTPV
                     EATVVLGKEDLTIKISDRGGGVPLRITDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGL
                     PISRLYAKYFQGDLNLYSMSGYGTDAIIYLKALSSESVEKLPVFNKSAFKHYQMSSEA
                     DDWCIPSREPKNLAKEKLAV"
     exon            247..388
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            389..460
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            461..645
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            646..732
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            733..810
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            811..887
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            888..986
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1097
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1211
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..3453
                     /gene="Pdk4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1723..1728
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pdk4"
                     /note="hexamer: AATAAA"
     polyA_site      1743
                     /gene="Pdk4"
ORIGIN      
        1 gagcacccgg gaccctggga ccacaacgca cttgctccct ctcgaccgcg ctcctgaccc
       61 gcagccctcg ccaaccctac ggatcctaac caccgccagc ctaggtgggc gtcaggatga
      121 aggcagcccg cttcgtgatg cgcagcgcca gctcgctgag cagcgccagc ctggtcccca
      181 gggaggtcga gctgttctcc cgctacagcc cgtccccgct gtccatgaag cagctgctgg
      241 actttggttc agaaaatgcc tgtgaaagaa cgtcctttgc ttttctgcgg caagagctgc
      301 ccgtccgcct ggccaatatc ctgaaggaga ttgacatcct gcctgaccgc ttagtgaaca
      361 ctccttcggt gcagctggtg aagagctggt atatccagag cctgatggat ttggtggagt
      421 tccatgagaa gagcccagaa gaccagaaag ccctgtcaga gtttgtagac acgctggtca
      481 aagttcgaaa cagacatcat aatgtggtcc ctacaatggc tcaaggcatc ctggagtata
      541 aagacacctg cacagtggac cccgttacca atcaaaatct tcagtatttt ttagaccggt
      601 tttacatgaa ccgcatttct actcggatgc tcatgaatca gcacatcctc atattcagtg
      661 actcaaagac gggaaaccca agccacattg gaagtatcga cccaaactgt gatgtggtag
      721 cagtagtcca agatgccttt gagtgtgcaa agatgctctg cgaccagtat tatctaacat
      781 cgccagaatt aaacctcaca caagtcaatg gaaaatttcc aggccaacca atccacattg
      841 tgtacgttcc ttcacacctt caccacatgc tcttcgaact cttcaagaat gccatgaggg
      901 ccacggtcga gcatcaagaa aaccgtcctt ccttgacccc agtagaggcc actgtcgtct
      961 tgggaaaaga agaccttaca atcaagattt ctgaccgagg aggcggtgtt cctctgagga
     1021 ttactgaccg cctctttagt tacacgtact ccactgctcc aacacctgtg atggacaatt
     1081 cccggaatgc ccctttggct ggttttggtt atggcttgcc aatttctcgt ctctacgcca
     1141 agtattttca aggagatctg aatctctact ctatgtcagg ttatgggaca gacgctatca
     1201 tctacttaaa ggctttatct tctgagtctg tagaaaagct cccagtcttt aacaagtcag
     1261 ccttcaaaca ttatcagatg agctccgaag ctgatgactg gtgtatccca agcagggaac
     1321 cgaagaacct ggcgaaggag aagctggcag tgtgaagcgg atgacgcctg acattttacg
     1381 ggatcaaagt gggtctgtgg cattgctgct tcgtgaatgt gtgtggactc tagtttccgc
     1441 aaaacaacgc aacacaaaac caagcaagca aaacacaaac acgagtacaa accttgacct
     1501 gatgagggac agagcttggt tggatgaccc gggagaagtc agggcagggc tccaggggat
     1561 aacaggtgtc ctgcttctcc tttggcaatg caaaatgact cctgactgtt ccaaatactg
     1621 aaaagaagtc tgcctctgag ttacagctct ttctcaacaa gtacagagtt tgaggcttgc
     1681 agttgcaaca gctggatgtt tggtggttct tgctgccagc caaataaatt ggtgtttagt
     1741 gaacattttc agtgtttccc cgccatgcaa agcttggcgc cttgggagaa atgtgtgtaa
     1801 atgtacattg tataggtatt agtgtgctct agaaaggaca ggatggaagg aatcaaagca
     1861 ctttatcgag cttgtggctg agcattgcag cctatgtgca aacccagagg aaaagtatct
     1921 ctgtcaagac agctccagta tcatgcagct ttttatgttt gcactcaaaa agccagtgcc
     1981 ttctggctgg tgccgaggct tgggtgaaat gttaaatatg cactgacctc agaaagtcga
     2041 gttcaaaagg gagataaaat tgccaaagtg atccaaggat tgtgcatgtt gggaaaccca
     2101 tatgagagaa aggattctca tacttagaac tttcctatga agaaatggtg gtaaactttc
     2161 tctacctaga agtagtggaa atttcaaggt catcttaaaa aagatgtgcg ttgtatattt
     2221 taactacatt ctctacactc taacattaac atatctattc aaatttgtct agttgccaat
     2281 tgtcttcaga gtgtgaaaat ttaaatcctt cttgaagtat ctttcgtgag agtagtatgg
     2341 aagtaaaacg ttctcatatc aggaggatgt catttgtgaa gcatggggac atcatgaact
     2401 agtgatgtgc gtgaggcttg ggaggctgaa gggtaaggat cagcgggagg ccatccatgt
     2461 aggagagaga attaaaacga ggagcgaggg aagcaatgga gagagggaag caagaaagga
     2521 accagaaggc tggcatcatc ctatttccca caggctaacc caagggatgc tctgtgcctt
     2581 tcctggggag ggaaggggat gaactggtag atttgaaagc agtatggctt cttctgtggg
     2641 tctccctctt actagacaag gtgaaatgat aattcgtgtc aaattaatgt gaaatttttt
     2701 tcctgcattg taatattatg aggcctgagt cgcagttgag tttgaaattt gtatttaatt
     2761 tcacagtgac ctagagctaa ggtgctcccg gttgtggcaa taggagccac aagtattttc
     2821 tttctttctt tcgttctttc tttctttctt tctttctttc tttctttctt tctttctttc
     2881 tttccttcct tccttccttc cttccttcct tccttccttc cttccttctt cctttccttt
     2941 tcttttctct tctcttcttt tcttttttct gtttcttttt ctttttttgc attgtagatg
     3001 ttgtccttaa aagatcaggg cagtgacttt cacagcagga ctttgactcc cacattggtt
     3061 gatcacacaa aactgtcagc atttgggtaa tctgatgtat agttgttttg ttgctgatgt
     3121 ttccattgaa atttcagctc tgagtttgtg cacatgaata cttacttgtg tttaccaaag
     3181 gtctaaggca tttggttact taacccaaat atcctgaact gtgcgtaaag taatagagaa
     3241 aagctttagg gtctcaatag tgtcacctgt gtaaatcaaa tcaaaatagc cttccctatt
     3301 atttatgaac ccatgggaga ctttaaactc ttgtagatag atgctaaatg cccaggccca
     3361 cttaacttat taatgtgtga attacattta tgtttttagt ttatatgcaa agaattgtga
     3421 taattttata ataaatattt ttattataat agt
//
