LOCUS       NM_175094               2527 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus pyruvate dehydrogenase complex, component X (Pdhx),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_175094
VERSION     NM_175094.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Bulusu,V., Prior,N., Snaebjornsson,M.T., Kuehne,A., Sonnen,K.F.,
            Kress,J., Stein,F., Schultz,C., Sauer,U. and Aulehla,A.
  TITLE     Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to
            Mouse Embryo Mesoderm Development
  JOURNAL   Dev Cell 40 (4), 331-341 (2017)
   PUBMED   28245920
REFERENCE   3  (bases 1 to 2527)
  AUTHORS   Duteil,D., Tosic,M., Lausecker,F., Nenseth,H.Z., Muller,J.M.,
            Urban,S., Willmann,D., Petroll,K., Messaddeq,N., Arrigoni,L.,
            Manke,T., Kornfeld,J.W., Bruning,J.C., Zagoriy,V., Meret,M.,
            Dengjel,J., Kanouni,T. and Schule,R.
  TITLE     Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose
            Tissue
  JOURNAL   Cell Rep 17 (4), 1008-1021 (2016)
   PUBMED   27760309
REFERENCE   4  (bases 1 to 2527)
  AUTHORS   Goguet-Rubio,P., Seyran,B., Gayte,L., Bernex,F., Sutter,A.,
            Delpech,H., Linares,L.K., Riscal,R., Repond,C., Rodier,G.,
            Kirsh,O., Touhami,J., Noel,J., Vincent,C., Pirot,N., Pavlovic,G.,
            Herault,Y., Sitbon,M., Pellerin,L., Sardet,C., Lacroix,M. and Le
            Cam,L.
  TITLE     E4F1-mediated control of pyruvate dehydrogenase activity is
            essential for skin homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 113 (39), 11004-11009 (2016)
   PUBMED   27621431
REFERENCE   5  (bases 1 to 2527)
  AUTHORS   Mathias,R.A., Greco,T.M., Oberstein,A., Budayeva,H.G.,
            Chakrabarti,R., Rowland,E.A., Kang,Y., Shenk,T. and Cristea,I.M.
  TITLE     Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase
            complex activity
  JOURNAL   Cell 159 (7), 1615-1625 (2014)
   PUBMED   25525879
REFERENCE   6  (bases 1 to 2527)
  AUTHORS   Hiromasa,Y., Fujisawa,T., Aso,Y. and Roche,T.E.
  TITLE     Organization of the cores of the mammalian pyruvate dehydrogenase
            complex formed by E2 and E2 plus the E3-binding protein and their
            capacities to bind the E1 and E3 components
  JOURNAL   J Biol Chem 279 (8), 6921-6933 (2004)
   PUBMED   14638692
REFERENCE   7  (bases 1 to 2527)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 2527)
  AUTHORS   Schacht,V., Ramirez,M.I., Hong,Y.K., Hirakawa,S., Feng,D.,
            Harvey,N., Williams,M., Dvorak,A.M., Dvorak,H.F., Oliver,G. and
            Detmar,M.
  TITLE     T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature
            formation and causes lymphedema
  JOURNAL   EMBO J 22 (14), 3546-3556 (2003)
   PUBMED   12853470
  REMARK    GeneRIF: T1a/podoplanin is first expressed in prox 1-positive
            lymphatic progenitor cells, with predominant localization in the
            luminal plasma membrane of lymphatic endothelial cells during later
            development.
REFERENCE   9  (bases 1 to 2527)
  AUTHORS   Gawin,B., Niederfuhr,A., Schumacher,N., Hummerich,H., Little,P.F.
            and Gessler,M.
  TITLE     A 7.5 Mb sequence-ready PAC contig and gene expression map of human
            chromosome 11p13-p14.1
  JOURNAL   Genome Res 9 (11), 1074-1086 (1999)
   PUBMED   10568747
REFERENCE   10 (bases 1 to 2527)
  AUTHORS   Johnson,M.T., Yang,H.S., Magnuson,T. and Patel,M.S.
  TITLE     Targeted disruption of the murine dihydrolipoamide dehydrogenase
            gene (Dld) results in perigastrulation lethality
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14512-14517 (1997)
   PUBMED   9405644
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK050433.1, AK047670.1 and BX635213.1.
            
            On Apr 27, 2010 this sequence version replaced NM_175094.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK047670.1, SRR1660811.90817.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1732              AK050433.1         2-1733
            1733-2498           AK047670.1         1731-2496
            2499-2527           BX635213.1         1-29                c
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 54.25 cM"
     gene            1..2527
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /note="pyruvate dehydrogenase complex, component X"
                     /db_xref="GeneID:27402"
                     /db_xref="MGI:MGI:1351627"
     exon            1..224
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1570
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /note="lipoyl-containing pyruvate dehydrogenase complex
                     component X; dihydrolipoamide dehydrogenase-binding
                     protein of pyruvate dehydrogenase complex (E3-binding
                     protein) (proX)"
                     /codon_start=1
                     /product="pyruvate dehydrogenase protein X component,
                     mitochondrial"
                     /protein_id="NP_780303.1"
                     /db_xref="CCDS:CCDS16473.1"
                     /db_xref="GeneID:27402"
                     /db_xref="MGI:MGI:1351627"
                     /translation="MAASWRLHCNQPLLRYLLGFSSRRSLGLAQGAAAWPVDRGASWR
                     WFHSTQLLQADPIKVLMPSLSPTMEQGNIVKWLRKEGEAVSAGDSLCEIETDKAVVTL
                     DANDDGILAKIVVEEGAKNIQLGSLIALMVEEGEDWKQVEIPKDVSAPPPVSKPPAPT
                     QPSPQPQIPCPARKEHKGTARFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALK
                     LVELKQMGKITESRPASAPPPSLSASVPPQATAGPSYPRPMTPPVSIPGQPNAAGTFT
                     EIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRRDLVKDDIKVSVNDFIIR
                     AAAVTLKQMPGVNVTWDGEGPKQLPSVDISVAVATDKGLITPIIKDAAAKGIQEIADS
                     VKVLSKKARDGKLMPEEYQGGSFSISNLGMFGIDEFAAVINPPQACILAVGRFRPVLK
                     LTEDEEGNPQLQQHQLITVTMSSDSRVVDDELATRFLETFKANLENPMRLG"
     exon            225..305
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     exon            306..406
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     exon            407..606
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     exon            607..705
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     exon            706..880
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1028
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1087
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1246
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1311
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..2526
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1769..1774
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /note="hexamer: ATTAAA"
     polyA_site      1789
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /note="major polyA site"
     regulatory      2487..2492
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /note="hexamer: AATAAA"
     polyA_site      2506
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
ORIGIN      
        1 ctggacgggc cttgaggccg ccaatctagt tgacttgcga gctttcccgg agaaagccgg
       61 gaagatggcg gcctcctgga ggcttcactg taaccagccg ctgcttcggt accttctggg
      121 atttagcagc cgtcgaagcc tgggactggc tcagggtgct gctgcttggc ctgtcgaccg
      181 tggagcaagt tggaggtggt ttcacagtac tcagctgctg caggcggatc ctattaaggt
      241 cctaatgcca tcactgtctc ctacgatgga gcaagggaac attgtgaaat ggctgagaaa
      301 ggaaggtgaa gctgtgagtg ctggagactc gttatgtgaa attgagacgg acaaggctgt
      361 ggtcacctta gatgcaaacg atgatggcat cttagccaag atagtggttg aggaaggtgc
      421 taaaaatata cagctgggtt cactcattgc tttgatggtt gaagaagggg aagattggaa
      481 acaggtggaa attcccaagg atgtcagtgc tccacctcca gtctcaaaac caccagctcc
      541 tactcagccc tctccacagc cgcagattcc ttgtcccgcc aggaaggagc acaaagggac
      601 ggcacggttc cgcttaagtc cagctgcccg caacattctg gaaaaacatt cattggacgc
      661 cagccagggc acagccacag gccctcgcgg gatcttcact aaagaagatg cactcaagtt
      721 agtcgagctg aaacagatgg gcaagattac tgaatccaga ccagcctcag ctcctccacc
      781 ctctctctca gcatctgtgc ctcctcaggc tacagccggg ccatcctatc ctcggccaat
      841 gaccccacca gtatccattc ctgggcaacc gaatgcagcg ggtacattca ccgaaattcc
      901 tgctagcaat attcgaagag ttatcgctaa gaggttaact gaatcaaaaa gtactgtgcc
      961 tcatgcctat gctactgctg actgtgacct tggcgcagtt ctaaaggtta gacgagatct
     1021 ggtcaaagat gacattaaag tctcagtaaa tgactttatc atcagagcag cagctgttac
     1081 tcttaaacaa atgcctgggg ttaatgtcac ctgggatgga gaaggcccaa agcagctgcc
     1141 ctctgttgac atttcagtgg ctgtggcaac agataaaggt ttaattacac caatcataaa
     1201 agatgcagcc gctaagggca ttcaggagat cgctgactct gtaaaggttc tgtcgaagaa
     1261 agcacgggat gggaaattga tgccagagga gtaccaagga ggatctttta gtatttccaa
     1321 cttggggatg tttggcatcg atgagttcgc tgcagtgatt aacccgcctc aggcctgtat
     1381 cttggccgtt gggagattcc gccccgtgct gaagctgacg gaggatgaag aggggaaccc
     1441 tcaactgcag cagcaccagc ttataacggt cacaatgtca agtgacagcc gagtggttga
     1501 tgatgaacta gctaccaggt ttcttgaaac tttcaaagca aacctagaga atcccatgcg
     1561 acttgggtag ccttcagaga ccaggcgctt gattgggtgt tcagtatgga gtagatgaag
     1621 tgcgtattta tttaaggcga aagctttgac ctgagctgtc ttcctctccc ctgtacatgt
     1681 actgtagaaa tgtgtgatga caaacggact aataaagaac atttggttgg tcagatccac
     1741 ttttaacctc tggtgctgta cagaaggcat taaactaatg taaactacat cctatgtttg
     1801 gttcatttga aattttggaa taattgtgaa tgtatgatat tgtaaaatta aaaaaatata
     1861 tagaactgtc ccttaatcat attgacgtta taactgggct tcaaagagat gtctgtctat
     1921 attggggcct ctcatttttt ataagctctt gatatacttg aacaatttaa tatgtacagt
     1981 tagtttgctc tggattagta tataagtaca ttttattagg ggttgtgaca acattattaa
     2041 acttttgtgt atataaagcc aggtgtctta gctagatata tttccagtct tttaactttg
     2101 acactgtgaa gctctaagga tacaatagga atgaagacag aggaagaaaa actaaagagt
     2161 cgtttgttca gagacctgac cttataaagt tgggggagca gctgggtttg tacctataac
     2221 ctcagccttt gagagagatt gagaaaggag gcccgctttg aggctagctg ggctacgcgg
     2281 tgaggccctg tctcacagag gcagggagag tgttttactg gaaaagctgc agttgatctc
     2341 aaattgagca gcgagctttc atgctcagtt ggaatctaat gaaataaatt ctcttagcat
     2401 acattgtaaa tttgtattgt tttaaataca gtattgtttg ctgaagcatt gtaatcaagt
     2461 ttgtattgta cagtcaaatg taagaaaata aaaaaattct agtctacttt gatatgacat
     2521 gcaaaaa
//
