LOCUS       NP_780303                501 aa            linear   ROD 19-SEP-2024
DEFINITION  pyruvate dehydrogenase protein X component, mitochondrial [Mus
            musculus].
ACCESSION   NP_780303
VERSION     NP_780303.1
DBSOURCE    REFSEQ: accession NM_175094.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 501)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 501)
  AUTHORS   Bulusu,V., Prior,N., Snaebjornsson,M.T., Kuehne,A., Sonnen,K.F.,
            Kress,J., Stein,F., Schultz,C., Sauer,U. and Aulehla,A.
  TITLE     Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to
            Mouse Embryo Mesoderm Development
  JOURNAL   Dev Cell 40 (4), 331-341 (2017)
   PUBMED   28245920
REFERENCE   3  (residues 1 to 501)
  AUTHORS   Duteil,D., Tosic,M., Lausecker,F., Nenseth,H.Z., Muller,J.M.,
            Urban,S., Willmann,D., Petroll,K., Messaddeq,N., Arrigoni,L.,
            Manke,T., Kornfeld,J.W., Bruning,J.C., Zagoriy,V., Meret,M.,
            Dengjel,J., Kanouni,T. and Schule,R.
  TITLE     Lsd1 Ablation Triggers Metabolic Reprogramming of Brown Adipose
            Tissue
  JOURNAL   Cell Rep 17 (4), 1008-1021 (2016)
   PUBMED   27760309
REFERENCE   4  (residues 1 to 501)
  AUTHORS   Goguet-Rubio,P., Seyran,B., Gayte,L., Bernex,F., Sutter,A.,
            Delpech,H., Linares,L.K., Riscal,R., Repond,C., Rodier,G.,
            Kirsh,O., Touhami,J., Noel,J., Vincent,C., Pirot,N., Pavlovic,G.,
            Herault,Y., Sitbon,M., Pellerin,L., Sardet,C., Lacroix,M. and Le
            Cam,L.
  TITLE     E4F1-mediated control of pyruvate dehydrogenase activity is
            essential for skin homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 113 (39), 11004-11009 (2016)
   PUBMED   27621431
REFERENCE   5  (residues 1 to 501)
  AUTHORS   Mathias,R.A., Greco,T.M., Oberstein,A., Budayeva,H.G.,
            Chakrabarti,R., Rowland,E.A., Kang,Y., Shenk,T. and Cristea,I.M.
  TITLE     Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase
            complex activity
  JOURNAL   Cell 159 (7), 1615-1625 (2014)
   PUBMED   25525879
REFERENCE   6  (residues 1 to 501)
  AUTHORS   Hiromasa,Y., Fujisawa,T., Aso,Y. and Roche,T.E.
  TITLE     Organization of the cores of the mammalian pyruvate dehydrogenase
            complex formed by E2 and E2 plus the E3-binding protein and their
            capacities to bind the E1 and E3 components
  JOURNAL   J Biol Chem 279 (8), 6921-6933 (2004)
   PUBMED   14638692
REFERENCE   7  (residues 1 to 501)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (residues 1 to 501)
  AUTHORS   Schacht,V., Ramirez,M.I., Hong,Y.K., Hirakawa,S., Feng,D.,
            Harvey,N., Williams,M., Dvorak,A.M., Dvorak,H.F., Oliver,G. and
            Detmar,M.
  TITLE     T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature
            formation and causes lymphedema
  JOURNAL   EMBO J 22 (14), 3546-3556 (2003)
   PUBMED   12853470
  REMARK    GeneRIF: T1a/podoplanin is first expressed in prox 1-positive
            lymphatic progenitor cells, with predominant localization in the
            luminal plasma membrane of lymphatic endothelial cells during later
            development.
REFERENCE   9  (residues 1 to 501)
  AUTHORS   Gawin,B., Niederfuhr,A., Schumacher,N., Hummerich,H., Little,P.F.
            and Gessler,M.
  TITLE     A 7.5 Mb sequence-ready PAC contig and gene expression map of human
            chromosome 11p13-p14.1
  JOURNAL   Genome Res 9 (11), 1074-1086 (1999)
   PUBMED   10568747
REFERENCE   10 (residues 1 to 501)
  AUTHORS   Johnson,M.T., Yang,H.S., Magnuson,T. and Patel,M.S.
  TITLE     Targeted disruption of the murine dihydrolipoamide dehydrogenase
            gene (Dld) results in perigastrulation lethality
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14512-14517 (1997)
   PUBMED   9405644
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK050433.1, AK047670.1 and BX635213.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK047670.1, SRR1660811.90817.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 54.25 cM"
     Protein         1..501
                     /product="pyruvate dehydrogenase protein X component,
                     mitochondrial"
                     /note="lipoyl-containing pyruvate dehydrogenase complex
                     component X; dihydrolipoamide dehydrogenase-binding
                     protein of pyruvate dehydrogenase complex (E3-binding
                     protein) (proX)"
                     /calculated_mol_wt=47948
     transit_peptide 1..53
                     /note="Mitochondrion. /evidence=ECO:0000250; propagated
                     from UniProtKB/Swiss-Prot (Q8BKZ9.1)"
                     /calculated_mol_wt=6069
     mat_peptide     54..501
                     /product="Pyruvate dehydrogenase protein X component,
                     mitochondrial. /id=PRO_0000020485"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BKZ9.1)"
                     /calculated_mol_wt=47948
     Region          58..500
                     /region_name="PDHac_trf_mito"
                     /note="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase, long form; TIGR01349"
                     /db_xref="CDD:273567"
     Region          145..176
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BKZ9.1)"
     Site            194
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:O00330; propagated from
                     UniProtKB/Swiss-Prot (Q8BKZ9.1)"
     Site            196
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:O00330; propagated from
                     UniProtKB/Swiss-Prot (Q8BKZ9.1)"
     Region          228..256
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BKZ9.1)"
     CDS             1..501
                     /gene="Pdhx"
                     /gene_synonym="E3bp; Pdx1"
                     /coded_by="NM_175094.5:65..1570"
                     /db_xref="CCDS:CCDS16473.1"
                     /db_xref="GeneID:27402"
                     /db_xref="MGI:MGI:1351627"
ORIGIN      
        1 maaswrlhcn qpllryllgf ssrrslglaq gaaawpvdrg aswrwfhstq llqadpikvl
       61 mpslsptmeq gnivkwlrke geavsagdsl ceietdkavv tldanddgil akivveegak
      121 niqlgslial mveegedwkq veipkdvsap ppvskppapt qpspqpqipc parkehkgta
      181 rfrlspaarn ilekhsldas qgtatgprgi ftkedalklv elkqmgkite srpasappps
      241 lsasvppqat agpsyprpmt ppvsipgqpn aagtfteipa snirrviakr lteskstvph
      301 ayatadcdlg avlkvrrdlv kddikvsvnd fiiraaavtl kqmpgvnvtw dgegpkqlps
      361 vdisvavatd kglitpiikd aaakgiqeia dsvkvlskka rdgklmpeey qggsfsisnl
      421 gmfgidefaa vinppqacil avgrfrpvlk ltedeegnpq lqqhqlitvt mssdsrvvdd
      481 elatrfletf kanlenpmrl g
//
