LOCUS       NM_024221               1485 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus pyruvate dehydrogenase (lipoamide) beta (Pdhb), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_024221 XM_904678
VERSION     NM_024221.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Jiang,X., Ji,S., Yuan,F., Li,T., Cui,S., Wang,W., Ye,X., Wang,R.,
            Chen,Y. and Zhu,S.
  TITLE     Pyruvate dehydrogenase B regulates myogenic differentiation via the
            FoxP1-Arih2 axis
  JOURNAL   J Cachexia Sarcopenia Muscle 14 (1), 606-621 (2023)
   PUBMED   36564038
  REMARK    GeneRIF: Pyruvate dehydrogenase B regulates myogenic
            differentiation via the FoxP1-Arih2 axis.
REFERENCE   3  (bases 1 to 1485)
  AUTHORS   Zhao,K., Huang,X., Zhao,W., Lu,B. and Yang,Z.
  TITLE     LONP1-mediated mitochondrial quality control safeguards metabolic
            shifts in heart development
  JOURNAL   Development 149 (6) (2022)
   PUBMED   35237793
REFERENCE   4  (bases 1 to 1485)
  AUTHORS   Moutaoufik,M.T., Malty,R., Amin,S., Zhang,Q., Phanse,S.,
            Gagarinova,A., Zilocchi,M., Hoell,L., Minic,Z., Gagarinova,M.,
            Aoki,H., Stockwell,J., Jessulat,M., Goebels,F., Broderick,K.,
            Scott,N.E., Vlasblom,J., Musso,G., Prasad,B., Lamantea,E.,
            Garavaglia,B., Rajput,A., Murayama,K., Okazaki,Y., Foster,L.J.,
            Bader,G.D., Cayabyab,F.S. and Babu,M.
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal
            Reprogramming Reveals Regulators of the Respirasome and
            Neurogenesis
  JOURNAL   iScience 19, 1114-1132 (2019)
   PUBMED   31536960
REFERENCE   5  (bases 1 to 1485)
  AUTHORS   Zurkirchen,L., Varum,S., Giger,S., Klug,A., Hausel,J., Bossart,R.,
            Zemke,M., Cantu,C., Atak,Z.K., Zamboni,N., Basler,K. and Sommer,L.
  TITLE     Yin Yang 1 sustains biosynthetic demands during brain development
            in a stage-specific manner
  JOURNAL   Nat Commun 10 (1), 2192 (2019)
   PUBMED   31097699
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1485)
  AUTHORS   Kikuchi,D., Minamishima,Y.A. and Nakayama,K.
  TITLE     Prolyl-hydroxylase PHD3 interacts with pyruvate dehydrogenase
            (PDH)-E1beta and regulates the cellular PDH activity
  JOURNAL   Biochem Biophys Res Commun 451 (2), 288-294 (2014)
   PUBMED   25088999
  REMARK    GeneRIF: PHD3 depletion did not affect the expression of the
            PDH-E1alpha, E1beta, and E2 subunits, or the phosphorylation status
            of E1alpha, but destabilized the PDH complex (PDC), resulting in
            less functional PDC.
REFERENCE   7  (bases 1 to 1485)
  AUTHORS   Uslu,V.V., Petretich,M., Ruf,S., Langenfeld,K., Fonseca,N.A.,
            Marioni,J.C. and Spitz,F.
  TITLE     Long-range enhancers regulating Myc expression are required for
            normal facial morphogenesis
  JOURNAL   Nat Genet 46 (7), 753-758 (2014)
   PUBMED   24859337
REFERENCE   8  (bases 1 to 1485)
  AUTHORS   Iso,T., Maeda,K., Hanaoka,H., Suga,T., Goto,K., Syamsunarno,M.R.,
            Hishiki,T., Nagahata,Y., Matsui,H., Arai,M., Yamaguchi,A.,
            Abumrad,N.A., Sano,M., Suematsu,M., Endo,K., Hotamisligil,G.S. and
            Kurabayashi,M.
  TITLE     Capillary endothelial fatty acid binding proteins 4 and 5 play a
            critical role in fatty acid uptake in heart and skeletal muscle
  JOURNAL   Arterioscler Thromb Vasc Biol 33 (11), 2549-2557 (2013)
   PUBMED   23968980
REFERENCE   9  (bases 1 to 1485)
  AUTHORS   Okajima,K., Korotchkina,L.G., Prasad,C., Rupar,T., Phillips,J.A.
            3rd, Ficicioglu,C., Hertecant,J., Patel,M.S. and Kerr,D.S.
  TITLE     Mutations of the E1beta subunit gene (PDHB) in four families with
            pyruvate dehydrogenase deficiency
  JOURNAL   Mol Genet Metab 93 (4), 371-380 (2008)
   PUBMED   18164639
REFERENCE   10 (bases 1 to 1485)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ159324.1, AK153058.1 and BY414585.1.
            
            On Jul 7, 2006 this sequence version replaced NM_024221.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK166631.1, SRR1660815.321377.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                CJ159324.1         5-50
            47-1478             AK153058.1         1-1432
            1479-1485           BY414585.1         377-383
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 4.74 cM"
     gene            1..1485
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /note="pyruvate dehydrogenase (lipoamide) beta"
                     /db_xref="GeneID:68263"
                     /db_xref="MGI:MGI:1915513"
     exon            1..102
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1140
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /EC_number="1.2.4.1"
                     /note="pyruvate dehydrogenase E1 component subunit beta,
                     mitochondrial; PDHE1-B"
                     /codon_start=1
                     /product="pyruvate dehydrogenase E1 component subunit
                     beta, mitochondrial precursor"
                     /protein_id="NP_077183.1"
                     /db_xref="CCDS:CCDS36800.1"
                     /db_xref="GeneID:68263"
                     /db_xref="MGI:MGI:1915513"
                     /translation="MAVVAGLVRGPLRQASGLLKRRFHRSAPAAVQLTVREAINQGMD
                     EELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMA
                     GLRPICEFMTFNFSMQAIDQVINSAAKTYYMSAGLQPVPIVFRGPNGASAGVAAQHSQ
                     CFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVMLENELMYGVAFELPAEAQS
                     KDFLIPIGKAKIERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDI
                     EAIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPY
                     AKVLEDNSVPQVKDIIFAVKKTLNI"
     exon            103..156
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            157..264
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            265..327
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            328..363
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            364..649
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            650..760
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            761..852
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            853..994
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1485
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1460..1465
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1484
                     /gene="Pdhb"
                     /gene_synonym="2610103L06Rik"
                     /note="major polyA site"
ORIGIN      
        1 agacgggctc tgcggcgctt ctgtgttgtc attcggcagc tagtagagga cacgggcaag
       61 atggcggtgg tggctggtct ggtgcgggga cccttgcggc aggcttccgg gctgctgaag
      121 aggcgttttc accgctcggc gcccgcagcg gtgcagttga cagttcgtga agctattaat
      181 caaggtatgg atgaagaact agaaagagat gaaaaagttt ttctgcttgg ggaagaagtt
      241 gcccagtatg acggtgcata caaggttagc agaggcctgt ggaagaaata cggtgacaag
      301 aggatcatcg acacccccat ctcagagatg ggctttgctg gaattgctgt tggtgcagct
      361 atggctgggt tgcggcccat ctgtgaattt atgaccttca atttctctat gcaagctatt
      421 gaccaggtta taaactcagc tgccaagact tactatatgt ccgctggcct tcagcctgtg
      481 cccatagtat tcagggggcc caatggcgcc tcagcaggtg tagctgctca gcactcgcaa
      541 tgctttgctg catggtatgg gcactgccca ggtttaaaag tggtcagccc ctggaattcc
      601 gaggatgcaa aaggacttat taaatcggcc attcgtgata ataacccagt ggtaatgcta
      661 gaaaatgaac tgatgtatgg agttgcattt gaacttcctg cagaagctca gtcaaaagat
      721 ttcctgattc ctattggaaa agccaaaatt gaaaggcaag ggacccacat cactgtagtt
      781 gcccattcaa gaccagtggg ccactgccta gaagctgcag ctgtattgtc taaggaggga
      841 attgaatgtg aggtaataaa tctgcgcacc atcagaccaa tggacatcga agccatagaa
      901 gccagtgtga tgaagacaaa tcatctcgtg actgtggaag gaggctggcc acaatttgga
      961 gtaggagctg agatttgtgc cagaattatg gaaggccctg cattcaactt ccttgatgcc
     1021 cctgccgtgc gtgtcactgg tgctgatgtc cctatgcctt acgcaaaggt cctagaagac
     1081 aactccgtac ctcaggtcaa agacatcata tttgcagtaa agaagactct gaacatctga
     1141 ttcggacttt gaatgtaaag tcattgaaat ctatttaaaa tccttgttgg gacttcacct
     1201 gatgtgtact gcgagactgc tagtcataaa gttaatgctg aagtgacagc aggtgtctga
     1261 actgtaagaa gtttcaatgt gcttatgagt ggaatgttgt ccactcccta ccctagatac
     1321 tctgctttgt ctgttactgt ttaagttaaa tttgccctct ttcactggaa ggggcaagat
     1381 aagaatgccc ccacagaagg taacttatat gtctaagaag tatattatct acatttgctg
     1441 tttaattgtt ttgataagga ataaagggga ttcccaccaa tgaag
//
