LOCUS       NM_008811               2352 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus pyruvate dehydrogenase E1 alpha 2 (Pdha2), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_008811
VERSION     NM_008811.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Hasan,P., Berezhnaya,E., Rodriguez-Prados,M., Weaver,D.,
            Bekeova,C., Cartes-Saavedra,B., Birch,E., Beyer,A.M., Santos,J.H.,
            Seifert,E.L., Elrod,J.W. and Hajnoczky,G.
  TITLE     MICU1 and MICU2 control mitochondrial calcium signaling in the
            mammalian heart
  JOURNAL   Proc Natl Acad Sci U S A 121 (35), e2402491121 (2024)
   PUBMED   39163336
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2352)
  AUTHORS   Mishra,A., Tavasoli,M., Sokolenko,S., McMaster,C.R. and
            Pasumarthi,K.B.S.
  TITLE     Atrial natriuretic peptide signaling co-regulates lipid metabolism
            and ventricular conduction system gene expression in the embryonic
            heart
  JOURNAL   iScience 27 (1), 108748 (2023)
   PUBMED   38235330
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2352)
  AUTHORS   Moutaoufik,M.T., Malty,R., Amin,S., Zhang,Q., Phanse,S.,
            Gagarinova,A., Zilocchi,M., Hoell,L., Minic,Z., Gagarinova,M.,
            Aoki,H., Stockwell,J., Jessulat,M., Goebels,F., Broderick,K.,
            Scott,N.E., Vlasblom,J., Musso,G., Prasad,B., Lamantea,E.,
            Garavaglia,B., Rajput,A., Murayama,K., Okazaki,Y., Foster,L.J.,
            Bader,G.D., Cayabyab,F.S. and Babu,M.
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal
            Reprogramming Reveals Regulators of the Respirasome and
            Neurogenesis
  JOURNAL   iScience 19, 1114-1132 (2019)
   PUBMED   31536960
REFERENCE   5  (bases 1 to 2352)
  AUTHORS   Bulusu,V., Prior,N., Snaebjornsson,M.T., Kuehne,A., Sonnen,K.F.,
            Kress,J., Stein,F., Schultz,C., Sauer,U. and Aulehla,A.
  TITLE     Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to
            Mouse Embryo Mesoderm Development
  JOURNAL   Dev Cell 40 (4), 331-341 (2017)
   PUBMED   28245920
REFERENCE   6  (bases 1 to 2352)
  AUTHORS   Takakubo,F. and Dahl,H.H.
  TITLE     Analysis of pyruvate dehydrogenase expression in embryonic mouse
            brain: localization and developmental regulation
  JOURNAL   Brain Res Dev Brain Res 77 (1), 63-76 (1994)
   PUBMED   7510589
REFERENCE   7  (bases 1 to 2352)
  AUTHORS   Iannello,R.C. and Dahl,H.H.
  TITLE     Transcriptional expression of a testis-specific variant of the
            mouse pyruvate dehydrogenase E1 alpha subunit
  JOURNAL   Biol Reprod 47 (1), 48-58 (1992)
   PUBMED   1637947
REFERENCE   8  (bases 1 to 2352)
  AUTHORS   Fitzgerald,J., Hutchison,W.M. and Dahl,H.H.
  TITLE     Isolation and characterisation of the mouse pyruvate dehydrogenase
            E1 alpha genes
  JOURNAL   Biochim Biophys Acta 1131 (1), 83-90 (1992)
   PUBMED   1581363
REFERENCE   9  (bases 1 to 2352)
  AUTHORS   Takakubo,F. and Dahl,H.H.
  TITLE     The expression pattern of the pyruvate dehydrogenase E1 alpha
            subunit genes during spermatogenesis in adult mouse
  JOURNAL   Exp Cell Res 199 (1), 39-49 (1992)
   PUBMED   1735460
REFERENCE   10 (bases 1 to 2352)
  AUTHORS   Brown,R.M., Dahl,H.H. and Brown,G.K.
  TITLE     Pyruvate dehydrogenase E1 alpha subunit genes in the mouse: mapping
            and comparison with human homologs
  JOURNAL   Somat Cell Mol Genet 16 (5), 487-492 (1990)
   PUBMED   2122529
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC100752.10.
            
            On Jun 16, 2009 this sequence version replaced NM_008811.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: M76728.1, AK076791.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2352              AC100752.10        109216-111567       c
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 65.3 cM"
     gene            1..2352
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
                     /note="pyruvate dehydrogenase E1 alpha 2"
                     /db_xref="GeneID:18598"
                     /db_xref="MGI:MGI:97533"
     exon            1..2352
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
                     /inference="alignment:Splign:2.1.0"
     CDS             611..1786
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
                     /EC_number="1.2.4.1"
                     /note="pyruvate dehydrogenase E1 component subunit alpha,
                     testis-specific form, mitochondrial; PDHE1-A type II;
                     PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT,
                     TESTIS-SPECIFIC FORM, MITOCHONDRIAL PRECURSOR (PDHE1-A
                     TYPE II)"
                     /codon_start=1
                     /product="pyruvate dehydrogenase E1 component subunit
                     alpha, testis-specific form, mitochondrial precursor"
                     /protein_id="NP_032837.1"
                     /db_xref="CCDS:CCDS17872.1"
                     /db_xref="GeneID:18598"
                     /db_xref="MGI:MGI:97533"
                     /translation="MRKMLTAVLSHVFSGMVQKPALRGLLSSLKFSNDATCDIKKCDL
                     YRLEEGPPTSTVLTRAEALKYYRTMQVIRRMELKADQLYKQKFIRGFCHLCDGQEACC
                     VGLEAGINPTDHVITSYRAHGFCYTRGLSVKSILAELTGRKGGCAKGKGGSMHMYGKN
                     FYGGNGIVGAQVPLGAGVAFACKYLKNGQVCLALYGDGAANQGQVFEAYNMSALWKLP
                     CVFICENNLYGMGTSNERSAASTDYHKKGFIIPGLRVNGMDILCVREATKFAADHCRS
                     GKGPIVMELQTYRYHGHSMSDPGISYRSREEVHNVRSKSDPIMLLRERIISNNLSNIE
                     ELKEIDADVKKEVEDAAQFATTDPEPAVEDIANYLYHQDPPFEVRGAHKWLKYKSHS"
     regulatory      1873..1878
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
                     /note="hexamer: AATAAA"
     regulatory      1877..1882
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
                     /note="hexamer: AATAAA"
     polyA_site      1895
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
                     /note="major polyA site"
     polyA_site      1900
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
                     /note="major polyA site"
     regulatory      1997..2002
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
                     /note="hexamer: ATTAAA"
     polyA_site      2021
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
     regulatory      2126..2131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
                     /note="hexamer: AATAAA"
     polyA_site      2149
                     /gene="Pdha2"
                     /gene_synonym="Pdhal"
ORIGIN      
        1 ctgtatttgt acatactttt caataaatgt taagatgttg ttattttttt cttttaaaaa
       61 cgaaaaggta gaagaaagaa tgatgtcatt ttgctccctc cctccttccc tcctgcctgc
      121 ccttcctcct tctcctcttt tcctcctctc caccgcccct cattttgagg ccaaagagac
      181 cacaaactga ctgagaatag gattcaagtc cggctcctac aaacctcaat gcctagtgtc
      241 tgagattaca ggtatgtgtg gcacagttac tgtctgagtt tagaggtgct gggatggaac
      301 ccaaaagctc tgcacagtag gctatgtccc taccctgcca ggactttttt gagttgtaca
      361 gggaaaagac actcagttgc tgtgtgagtg gcaaccatga tgttaaatag gaggaaagtg
      421 tgggagatcc tccctttcca gttagaagca tcagaggagc aggccccgtg ggcgtggctt
      481 ccagcttgct gacgtaggca acgcgtttgc atcccgttat tgttgcatca gaggagctcg
      541 gcagccatct taaaagccac tgagtgatcg ttgggagccg agccgctacc gttgtgcctc
      601 gcgtttctcc atgaggaaaa tgctgaccgc tgtgctgtct cacgtatttt cgggaatggt
      661 ccaaaagcca gctctcagag gactgctgtc atctctgaag ttctccaacg acgccacctg
      721 tgacattaag aaatgtgacc tgtaccggct ggaggagggc ccaccgacct ccaccgtgct
      781 cacccgagcc gaggccctca agtactaccg gaccatgcag gtaattcggc gcatggagtt
      841 gaaggccgac cagctgtata agcagaaatt catccgtggt ttctgtcacc tgtgtgatgg
      901 gcaggaagcc tgctgcgtgg ggctggaggc agggataaat cccacggatc acgtcatcac
      961 gtcctaccgg gctcatggct tctgctacac gcgaggactg tccgtgaagt ccattctcgc
     1021 cgagctgact ggacgcaaag gaggctgtgc taaaggcaag ggaggctcca tgcacatgta
     1081 cggcaagaac ttctacggtg gcaatggcat tgttggggcc caggtacccc tgggagctgg
     1141 tgtggctttt gcctgtaaat acctgaagaa tggtcaggtc tgcttggctt tgtacggcga
     1201 tggtgcggct aaccaagggc aggtattcga agcatacaat atgtcagcct tgtggaaatt
     1261 accctgtgtt ttcatctgtg agaataacct ctatggaatg ggaacctcca acgagagatc
     1321 agcagccagt actgattacc acaagaaagg ttttattatc cccggactga gggtgaatgg
     1381 gatggatatt ctctgtgttc gggaggcaac caagtttgca gctgatcact gcagatctgg
     1441 aaaggggccc attgtgatgg agctgcagac ctaccgttat catggacaca gtatgagcga
     1501 cccagggatc agttatcgtt cacgagaaga agttcataac gtgagaagta agagtgatcc
     1561 tataatgctg ctccgagaga gaattatcag caacaacctc agcaatattg aagaattgaa
     1621 agaaattgat gcagatgtga agaaagaggt ggaggacgca gctcagtttg ctacgactga
     1681 tccagaacca gctgtggaag atatagccaa ttacctctac caccaagatc caccttttga
     1741 agtccgtggt gcacataagt ggctcaagta taagtcccac agttagatag atgttaccta
     1801 tacatttgtt aaattttttt tcagtgggac atttatggtg tactctagga aacttcaact
     1861 ttgttaagga ggaataaata aaacgacatt gcagacaaaa gtcttataaa cctttataaa
     1921 gatagattcc tgagttatta aggagattag aagatataaa tttgtttaaa ggagatgttc
     1981 cactttctgt tttaacatta aaagcattgt gttgcatact actatgaata tcttttagac
     2041 tatttcaaat ttataaaatt ataatagaaa aaacgggtta aattccccaa tttggcatag
     2101 tagtttcatt tggttttagt tttgaaataa atacttttat ttaaaagaag tagatttttg
     2161 attttttctt cttgtttgat gagtttgtca aaaattaaaa gtgaggagga tggtttcaat
     2221 tctgtccccg cccccccctc accaaatacc tgattatgtt cccctttccc cctccatgtc
     2281 caatctctga cccaggttcc tccctccctc tgtcccctgt ggttgatttt tcctcctaag
     2341 taggattcag gc
//
