LOCUS       NM_008804               2045 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus phosphodiesterase 9A (Pde9a), transcript variant 1,
            mRNA.
ACCESSION   NM_008804
VERSION     NM_008804.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ceddia,R.P., Liu,D., Shi,F., Crowder,M.K., Mishra,S., Kass,D.A. and
            Collins,S.
  TITLE     Increased Energy Expenditure and Protection From Diet-Induced
            Obesity in Mice Lacking the cGMP-Specific Phosphodiesterase PDE9
  JOURNAL   Diabetes 70 (12), 2823-2836 (2021)
   PUBMED   34620617
  REMARK    GeneRIF: Increased Energy Expenditure and Protection From
            Diet-Induced Obesity in Mice Lacking the cGMP-Specific
            Phosphodiesterase PDE9.
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Mishra,S., Sadagopan,N., Dunkerly-Eyring,B., Rodriguez,S.,
            Sarver,D.C., Ceddia,R.P., Murphy,S.A., Knutsdottir,H., Jani,V.P.,
            Ashok,D., Oeing,C.U., O'Rourke,B., Gangoiti,J.A., Sears,D.D.,
            Wong,G.W., Collins,S. and Kass,D.A.
  TITLE     Inhibition of phosphodiesterase type 9 reduces obesity and
            cardiometabolic syndrome in mice
  JOURNAL   J Clin Invest 131 (21) (2021)
   PUBMED   34618683
  REMARK    GeneRIF: Inhibition of phosphodiesterase type 9 reduces obesity and
            cardiometabolic syndrome in mice.
REFERENCE   3  (bases 1 to 2045)
  AUTHORS   Kolb,T.M., Johnston,L., Damarla,M., Kass,D.A. and Hassoun,P.M.
  TITLE     PDE9A deficiency does not prevent chronic-hypoxic pulmonary
            hypertension in mice
  JOURNAL   Physiol Rep 9 (18), e15057 (2021)
   PUBMED   34569183
  REMARK    GeneRIF: PDE9A deficiency does not prevent chronic-hypoxic
            pulmonary hypertension in mice.
REFERENCE   4  (bases 1 to 2045)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2045)
  AUTHORS   Rattner,A., Terrillion,C.E., Jou,C., Kleven,T., Hu,S.F.,
            Williams,J., Hou,Z., Aggarwal,M., Mori,S., Shin,G., Goff,L.A.,
            Witter,M.P., Pletnikov,M., Fenton,A.A. and Nathans,J.
  TITLE     Developmental, cellular, and behavioral phenotypes in a mouse model
            of congenital hypoplasia of the dentate gyrus
  JOURNAL   Elife 9, e62766 (2020)
   PUBMED   33084572
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2045)
  AUTHORS   Liddie,S., Anderson,K.L., Paz,A. and Itzhak,Y.
  TITLE     The effect of phosphodiesterase inhibitors on the extinction of
            cocaine-induced conditioned place preference in mice
  JOURNAL   J Psychopharmacol 26 (10), 1375-1382 (2012)
   PUBMED   22596207
  REMARK    GeneRIF: Data suggest that increases in hippocampal and amygdalar
            cGMP levels via blockade of PDE9 have prominent roles in
            consolidation of extinction learning (here, prevention of learning
            disorder due to cocaine neurotoxicity).
REFERENCE   7  (bases 1 to 2045)
  AUTHORS   Omori,K. and Kotera,J.
  TITLE     Overview of PDEs and their regulation
  JOURNAL   Circ Res 100 (3), 309-327 (2007)
   PUBMED   17307970
  REMARK    Review article
REFERENCE   8  (bases 1 to 2045)
  AUTHORS   Guipponi,M., Scott,H.S., Kudoh,J., Kawasaki,K., Shibuya,K.,
            Shintani,A., Asakawa,S., Chen,H., Lalioti,M.D., Rossier,C.,
            Minoshima,S., Shimizu,N. and Antonarakis,S.E.
  TITLE     Identification and characterization of a novel cyclic nucleotide
            phosphodiesterase gene (PDE9A) that maps to 21q22.3: alternative
            splicing of mRNA transcripts, genomic structure and sequence
  JOURNAL   Hum Genet 103 (4), 386-392 (1998)
   PUBMED   9856478
REFERENCE   9  (bases 1 to 2045)
  AUTHORS   Fisher,D.A., Smith,J.F., Pillar,J.S., St Denis,S.H. and Cheng,J.B.
  TITLE     Isolation and characterization of PDE9A, a novel human
            cGMP-specific phosphodiesterase
  JOURNAL   J Biol Chem 273 (25), 15559-15564 (1998)
   PUBMED   9624146
  REMARK    GeneRIF: This paper describes the human Pde9 gene but discusses the
            comparisons between the mouse and human Pde9 genes.
REFERENCE   10 (bases 1 to 2045)
  AUTHORS   Soderling,S.H., Bayuga,S.J. and Beavo,J.A.
  TITLE     Identification and characterization of a novel family of cyclic
            nucleotide phosphodiesterases
  JOURNAL   J Biol Chem 273 (25), 15553-15558 (1998)
   PUBMED   9624145
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154652.2 and AC166172.1.
            
            On Aug 13, 2022 this sequence version replaced NM_008804.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030509.1, AK168143.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               AC154652.2         154444-154546
            104-174             AC166172.1         152707-152777       c
            175-252             AC166172.1         151521-151598       c
            253-296             AC166172.1         146590-146633       c
            297-351             AC166172.1         123658-123712       c
            352-422             AC166172.1         122970-123040       c
            423-507             AC166172.1         118427-118511       c
            508-586             AC166172.1         113398-113476       c
            587-661             AC166172.1         111750-111824       c
            662-748             AC166172.1         107717-107803       c
            749-853             AC166172.1         106873-106977       c
            854-936             AC166172.1         106617-106699       c
            937-1093            AC166172.1         105151-105307       c
            1094-1207           AC166172.1         100410-100523       c
            1208-1312           AC166172.1         96980-97084         c
            1313-1441           AC166172.1         96069-96197         c
            1442-1537           AC166172.1         95024-95119         c
            1538-1659           AC166172.1         93599-93720         c
            1660-2045           AC166172.1         90568-90953         c
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 16.23 cM"
     gene            1..2045
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /note="phosphodiesterase 9A"
                     /db_xref="GeneID:18585"
                     /db_xref="MGI:MGI:1277179"
     exon            1..103
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1639
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /EC_number="3.1.4.35"
                     /note="isoform 1 is encoded by transcript variant 1; high
                     affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A"
                     /codon_start=1
                     /product="high affinity cGMP-specific 3',5'-cyclic
                     phosphodiesterase 9A isoform 1"
                     /protein_id="NP_032830.3"
                     /db_xref="CCDS:CCDS28605.1"
                     /db_xref="GeneID:18585"
                     /db_xref="MGI:MGI:1277179"
                     /translation="MGAGSSSYRPKAIYLDIDGRIQKVVFSKYCNSSDIMDLFCIATG
                     LPRNTTISLLTTDDAMVSIDPTMPANSERTPYKVRPVAVKQVSEREELIQGVLAQVAE
                     QFSRAFKINELKAEVANHLAVLEKRVELEGLKVVEIEKCKSDIKKMREELAARNSRTN
                     CPCKYSFLDNKKLTPRRDVPTYPKYLLSPETIEALRKPTFDVWLWEPNEMLSCLEHMY
                     HDLGLVRDFSINPITLRRWLLCVHDNYRNNPFHNFRHCFCVTQMMYSMVWLCGLQEKF
                     SQMDILVLMTAAICHDLDHPGYNNTYQINARTELAVRYNDISPLENHHCAIAFQILAR
                     PECNIFASVPPEGFRQIRQGMITLILATDMARHAEIMDSFKEKMENFDYSNEEHLTLL
                     KMILIKCCDISNEVRPMEVAEPWVDCLLEEYFMQSDREKSEGLPVAPFMDRDKVTKAT
                     AQIGFIKFVLIPMFETVTKLFPVVEETMLRPLWESREHYEELKQLDDAMKELQKKTES
                     LTSGAPENTTEKNRDAKDSEGHSPPN"
     exon            104..174
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            175..252
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            253..296
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            297..351
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            352..422
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            423..507
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            508..586
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            587..661
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            662..748
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            749..853
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            854..936
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1093
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1207
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1312
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1441
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1442..1537
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1659
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1660..2045
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1875..1880
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /note="hexamer: AATATA"
     polyA_site      1894
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /note="major polyA site"
     regulatory      2019..2024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /note="hexamer: ATTAAA"
     polyA_site      2045
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /note="major polyA site"
ORIGIN      
        1 aaggtgaaaa agtccaagtg cagctgcggg cgcgatgggg gccggctcct caagctaccg
       61 gcccaaggcc atctacctag acatcgatgg acgcatccag aaggtggttt tcagcaagta
      121 ctgcaactcc agtgacatca tggacctgtt ctgtatcgcc accggcctgc ctcggaacac
      181 caccatctcc cttttaacca cagacgacgc catggtctcc atcgatccca ccatgcctgc
      241 gaattcagag cgcactccct acaaggtgag acctgtggct gtgaagcaag tgtctgaacg
      301 agaagaactt atccagggcg tgctggccca ggtggcggaa caattttcca gagcgtttaa
      361 gatcaacgag ctgaaagccg aagttgcaaa tcacctggcc gtgctggaga aacgggtgga
      421 attggaagga cttaaagtgg tggagatcga aaaatgcaag agtgacatta aaaagatgcg
      481 ggaggagttg gcagctagga acagcaggac caactgtcca tgtaaataca gttttttgga
      541 taacaagaag ttgacacctc gacgtgatgt ccccacttac cccaagtacc tgctctcccc
      601 agagaccatc gaagccctac ggaagcccac ctttgatgtc tggctttggg aacccaacga
      661 gatgctgagc tgtttagaac atatgtacca cgaccttggt ctggtcaggg acttcagcat
      721 caacccaatc acgctccgca ggtggctgct ctgtgtgcat gacaactaca ggaacaaccc
      781 cttccacaac ttccggcact gcttctgtgt gacacagatg atgtacagta tggtctggct
      841 ctgtggcctc caggagaagt tttcccagat ggacatcttg gtcctgatga ctgcagccat
      901 ctgccatgac ctggaccacc cagggtacaa caatacatac cagatcaatg cccgcacgga
      961 actcgctgtg cgctacaacg acatctcacc gctggagaac caccactgcg ccattgcctt
     1021 ccagatcctg gcaagacctg agtgcaacat cttcgccagt gtgccacccg agggcttcag
     1081 gcagatcagg caggggatga tcacattgat cctggctacc gacatggcaa ggcatgctga
     1141 aattatggat tctttcaaag aaaagatgga gaattttgac tacagcaacg aggagcacct
     1201 gaccctgctg aagatgattc tcataaaatg ctgtgatatc tccaatgaag tccgtcccat
     1261 ggaggtggca gaaccgtggg tggactgttt actggaagaa tattttatgc agagtgaccg
     1321 tgagaagtcc gaaggccttc ctgtggcccc attcatggac cgagacaaag tgaccaaagc
     1381 aacagcccaa attgggttca tcaagtttgt cctgatccca atgtttgaaa cagtgaccaa
     1441 gctcttcccc gttgttgagg agaccatgct gcggccgctc tgggagtccc gagaacacta
     1501 cgaggagctg aagcagctgg acgatgccat gaaggagttg cagaagaaga cagagagctt
     1561 aacatctggg gccccggaaa acaccacaga gaagaacaga gatgcaaaag acagtgaagg
     1621 ccattctcct ccaaactgat ggacatctca atgtgtgcac agactgtacc agttagagca
     1681 gatgaattgt ggcctgtgag tggacagagc caagcgaggc ttcccaggat cttccacaca
     1741 aggatggtca cgcccagacg acccctgatg acctgctaca gaccatgttt tctaagaacc
     1801 attttgttca ctgatataaa aacaggaatt cacgatgctg tacagaattt ttatttttaa
     1861 actgtctttt aaataatata ttcttataca gaaatgggtc tgtatttttt ttctttgggt
     1921 tttggactcg ggcttccaag aactctggtt ttccccttgg atgaatcttc atgtggatga
     1981 accgtgggat gggatgtgag ggatcatgta aattccacat taaatgtcag aacggtgccc
     2041 gagta
//
