LOCUS       NM_001410689            1837 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus phosphodiesterase 9A (Pde9a), transcript variant 5,
            mRNA.
ACCESSION   NM_001410689
VERSION     NM_001410689.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ceddia,R.P., Liu,D., Shi,F., Crowder,M.K., Mishra,S., Kass,D.A. and
            Collins,S.
  TITLE     Increased Energy Expenditure and Protection From Diet-Induced
            Obesity in Mice Lacking the cGMP-Specific Phosphodiesterase PDE9
  JOURNAL   Diabetes 70 (12), 2823-2836 (2021)
   PUBMED   34620617
  REMARK    GeneRIF: Increased Energy Expenditure and Protection From
            Diet-Induced Obesity in Mice Lacking the cGMP-Specific
            Phosphodiesterase PDE9.
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Mishra,S., Sadagopan,N., Dunkerly-Eyring,B., Rodriguez,S.,
            Sarver,D.C., Ceddia,R.P., Murphy,S.A., Knutsdottir,H., Jani,V.P.,
            Ashok,D., Oeing,C.U., O'Rourke,B., Gangoiti,J.A., Sears,D.D.,
            Wong,G.W., Collins,S. and Kass,D.A.
  TITLE     Inhibition of phosphodiesterase type 9 reduces obesity and
            cardiometabolic syndrome in mice
  JOURNAL   J Clin Invest 131 (21) (2021)
   PUBMED   34618683
  REMARK    GeneRIF: Inhibition of phosphodiesterase type 9 reduces obesity and
            cardiometabolic syndrome in mice.
REFERENCE   3  (bases 1 to 1837)
  AUTHORS   Kolb,T.M., Johnston,L., Damarla,M., Kass,D.A. and Hassoun,P.M.
  TITLE     PDE9A deficiency does not prevent chronic-hypoxic pulmonary
            hypertension in mice
  JOURNAL   Physiol Rep 9 (18), e15057 (2021)
   PUBMED   34569183
  REMARK    GeneRIF: PDE9A deficiency does not prevent chronic-hypoxic
            pulmonary hypertension in mice.
REFERENCE   4  (bases 1 to 1837)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1837)
  AUTHORS   Rattner,A., Terrillion,C.E., Jou,C., Kleven,T., Hu,S.F.,
            Williams,J., Hou,Z., Aggarwal,M., Mori,S., Shin,G., Goff,L.A.,
            Witter,M.P., Pletnikov,M., Fenton,A.A. and Nathans,J.
  TITLE     Developmental, cellular, and behavioral phenotypes in a mouse model
            of congenital hypoplasia of the dentate gyrus
  JOURNAL   Elife 9, e62766 (2020)
   PUBMED   33084572
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1837)
  AUTHORS   Liddie,S., Anderson,K.L., Paz,A. and Itzhak,Y.
  TITLE     The effect of phosphodiesterase inhibitors on the extinction of
            cocaine-induced conditioned place preference in mice
  JOURNAL   J Psychopharmacol 26 (10), 1375-1382 (2012)
   PUBMED   22596207
  REMARK    GeneRIF: Data suggest that increases in hippocampal and amygdalar
            cGMP levels via blockade of PDE9 have prominent roles in
            consolidation of extinction learning (here, prevention of learning
            disorder due to cocaine neurotoxicity).
REFERENCE   7  (bases 1 to 1837)
  AUTHORS   Omori,K. and Kotera,J.
  TITLE     Overview of PDEs and their regulation
  JOURNAL   Circ Res 100 (3), 309-327 (2007)
   PUBMED   17307970
  REMARK    Review article
REFERENCE   8  (bases 1 to 1837)
  AUTHORS   Guipponi,M., Scott,H.S., Kudoh,J., Kawasaki,K., Shibuya,K.,
            Shintani,A., Asakawa,S., Chen,H., Lalioti,M.D., Rossier,C.,
            Minoshima,S., Shimizu,N. and Antonarakis,S.E.
  TITLE     Identification and characterization of a novel cyclic nucleotide
            phosphodiesterase gene (PDE9A) that maps to 21q22.3: alternative
            splicing of mRNA transcripts, genomic structure and sequence
  JOURNAL   Hum Genet 103 (4), 386-392 (1998)
   PUBMED   9856478
REFERENCE   9  (bases 1 to 1837)
  AUTHORS   Fisher,D.A., Smith,J.F., Pillar,J.S., St Denis,S.H. and Cheng,J.B.
  TITLE     Isolation and characterization of PDE9A, a novel human
            cGMP-specific phosphodiesterase
  JOURNAL   J Biol Chem 273 (25), 15559-15564 (1998)
   PUBMED   9624146
  REMARK    GeneRIF: This paper describes the human Pde9 gene but discusses the
            comparisons between the mouse and human Pde9 genes.
REFERENCE   10 (bases 1 to 1837)
  AUTHORS   Soderling,S.H., Bayuga,S.J. and Beavo,J.A.
  TITLE     Identification and characterization of a novel family of cyclic
            nucleotide phosphodiesterases
  JOURNAL   J Biol Chem 273 (25), 15553-15558 (1998)
   PUBMED   9624145
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC166172.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13861859.4409014.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-92                AC166172.1         133670-133761       c
            93-147              AC166172.1         123658-123712       c
            148-218             AC166172.1         122970-123040       c
            219-303             AC166172.1         118427-118511       c
            304-382             AC166172.1         113398-113476       c
            383-457             AC166172.1         111750-111824       c
            458-544             AC166172.1         107717-107803       c
            545-649             AC166172.1         106873-106977       c
            650-732             AC166172.1         106617-106699       c
            733-889             AC166172.1         105151-105307       c
            890-1003            AC166172.1         100410-100523       c
            1004-1108           AC166172.1         96980-97084         c
            1109-1237           AC166172.1         96069-96197         c
            1238-1333           AC166172.1         95024-95119         c
            1334-1455           AC166172.1         93599-93720         c
            1456-1837           AC166172.1         90569-90950         c
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 16.23 cM"
     gene            1..1837
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /note="phosphodiesterase 9A"
                     /db_xref="GeneID:18585"
                     /db_xref="MGI:MGI:1277179"
     exon            1..92
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            93..147
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            148..218
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            219..303
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     CDS             272..1435
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /EC_number="3.1.4.35"
                     /note="isoform 4 is encoded by transcript variant 5; high
                     affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A"
                     /codon_start=1
                     /product="high affinity cGMP-specific 3',5'-cyclic
                     phosphodiesterase 9A isoform 4"
                     /protein_id="NP_001397618.1"
                     /db_xref="GeneID:18585"
                     /db_xref="MGI:MGI:1277179"
                     /translation="MREELAARNSRTNCPCKYSFLDNKKLTPRRDVPTYPKYLLSPET
                     IEALRKPTFDVWLWEPNEMLSCLEHMYHDLGLVRDFSINPITLRRWLLCVHDNYRNNP
                     FHNFRHCFCVTQMMYSMVWLCGLQEKFSQMDILVLMTAAICHDLDHPGYNNTYQINAR
                     TELAVRYNDISPLENHHCAIAFQILARPECNIFASVPPEGFRQIRQGMITLILATDMA
                     RHAEIMDSFKEKMENFDYSNEEHLTLLKMILIKCCDISNEVRPMEVAEPWVDCLLEEY
                     FMQSDREKSEGLPVAPFMDRDKVTKATAQIGFIKFVLIPMFETVTKLFPVVEETMLRP
                     LWESREHYEELKQLDDAMKELQKKTESLTSGAPENTTEKNRDAKDSEGHSPPN"
     exon            304..382
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            383..457
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            458..544
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            545..649
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            650..732
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            733..889
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1003
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1108
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1237
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1333
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1455
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1837
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1668..1673
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /note="hexamer: AATATA"
     polyA_site      1687
                     /gene="Pde9a"
                     /gene_synonym="PDE9A1"
                     /note="major polyA site"
ORIGIN      
        1 cagtcttagc acatccccac aggctgttta gaaggtagac tggtttggag ggcagctgca
       61 gggcctggga gagacaagaa gtgggacagg agaacgagaa gaacttatcc agggcgtgct
      121 ggcccaggtg gcggaacaat tttccagagc gtttaagatc aacgagctga aagccgaagt
      181 tgcaaatcac ctggccgtgc tggagaaacg ggtggaattg gaaggactta aagtggtgga
      241 gatcgaaaaa tgcaagagtg acattaaaaa gatgcgggag gagttggcag ctaggaacag
      301 caggaccaac tgtccatgta aatacagttt tttggataac aagaagttga cacctcgacg
      361 tgatgtcccc acttacccca agtacctgct ctccccagag accatcgaag ccctacggaa
      421 gcccaccttt gatgtctggc tttgggaacc caacgagatg ctgagctgtt tagaacatat
      481 gtaccacgac cttggtctgg tcagggactt cagcatcaac ccaatcacgc tccgcaggtg
      541 gctgctctgt gtgcatgaca actacaggaa caaccccttc cacaacttcc ggcactgctt
      601 ctgtgtgaca cagatgatgt acagtatggt ctggctctgt ggcctccagg agaagttttc
      661 ccagatggac atcttggtcc tgatgactgc agccatctgc catgacctgg accacccagg
      721 gtacaacaat acataccaga tcaatgcccg cacggaactc gctgtgcgct acaacgacat
      781 ctcaccgctg gagaaccacc actgcgccat tgccttccag atcctggcaa gacctgagtg
      841 caacatcttc gccagtgtgc cacccgaggg cttcaggcag atcaggcagg ggatgatcac
      901 attgatcctg gctaccgaca tggcaaggca tgctgaaatt atggattctt tcaaagaaaa
      961 gatggagaat tttgactaca gcaacgagga gcacctgacc ctgctgaaga tgattctcat
     1021 aaaatgctgt gatatctcca atgaagtccg tcccatggag gtggcagaac cgtgggtgga
     1081 ctgtttactg gaagaatatt ttatgcagag tgaccgtgag aagtccgaag gccttcctgt
     1141 ggccccattc atggaccgag acaaagtgac caaagcaaca gcccaaattg ggttcatcaa
     1201 gtttgtcctg atcccaatgt ttgaaacagt gaccaagctc ttccccgttg ttgaggagac
     1261 catgctgcgg ccgctctggg agtcccgaga acactacgag gagctgaagc agctggacga
     1321 tgccatgaag gagttgcaga agaagacaga gagcttaaca tctggggccc cggaaaacac
     1381 cacagagaag aacagagatg caaaagacag tgaaggccat tctcctccaa actgatggac
     1441 atctcaatgt gtgcaactgt accagttaga gcagatgaat tgtggcctgt gagtggacag
     1501 agccaagcga ggcttcccag gatcttccac acaaggatgg tcacgcccag acgacccctg
     1561 atgacctgct acagaccatg ttttctaaga accattttgt tcactgatat aaaaacagga
     1621 attcacgatg ctgtacagaa tttttatttt taaactgtct tttaaataat atattcttat
     1681 acagaaatgg gtctgtattt tttttctttg ggttttggac tcgggcttcc aagaactctg
     1741 gttttcccct tggatgaatc ttcatgtgga tgaaccgtgg gatgggatgt gagggatcat
     1801 gtaaattcca cattaaatgt cagaacggtg cccgagt
//
