LOCUS       NM_008803               4135 bp    mRNA    linear   ROD 05-AUG-2024
DEFINITION  Mus musculus phosphodiesterase 8A (Pde8a), mRNA.
ACCESSION   NM_008803 XM_911994
VERSION     NM_008803.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Qiu,N.Z., Hou,H.M., Guo,T.Y., Lv,Y.L., Zhou,Y., Zhang,F.F.,
            Zhang,F., Wang,X.D., Chen,W., Gao,Y.F., Chen,M.H., Zhang,X.H.,
            Zhang,H.T. and Wang,H.
  TITLE     Phosphodiesterase 8 (PDE8): Distribution and Cellular Expression
            and Association with Alzheimer's Disease
  JOURNAL   Neurochem Res 49 (8), 1993-2004 (2024)
   PUBMED   38782837
  REMARK    GeneRIF: Phosphodiesterase 8 (PDE8): Distribution and Cellular
            Expression and Association with Alzheimer's Disease.
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Qasim,H., Rajaei,M., Xu,Y., Reyes-Alcaraz,A., Abdelnasser,H.Y.,
            Stewart,M.D., Lahiri,S.K., Wehrens,X.H.T. and McConnell,B.K.
  TITLE     AKAP12 Upregulation Associates With PDE8A to Accelerate Cardiac
            Dysfunction
  JOURNAL   Circ Res 134 (8), 1006-1022 (2024)
   PUBMED   38506047
  REMARK    GeneRIF: AKAP12 Upregulation Associates With PDE8A to Accelerate
            Cardiac Dysfunction.
REFERENCE   3  (bases 1 to 4135)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 4135)
  AUTHORS   Basole,C.P., Nguyen,R.K., Lamothe,K., Vang,A., Clark,R.,
            Baillie,G.S., Epstein,P.M. and Brocke,S.
  TITLE     PDE8 controls CD4+ T cell motility through the PDE8A-Raf-1 kinase
            signaling complex
  JOURNAL   Cell Signal 40, 62-72 (2017)
   PUBMED   28851628
  REMARK    GeneRIF: our studies demonstrate that PDE8A inhibition by enzymatic
            inhibitors or PDE8A-Raf-1 kinase complex disruptors decreases Teff
            cell adhesion and migration under flow, and represents a novel
            approach to target T cells in inflammation
REFERENCE   5  (bases 1 to 4135)
  AUTHORS   Isidori,A.M., Cornacchione,M., Barbagallo,F., Di Grazia,A.,
            Barrios,F., Fassina,L., Monaco,L., Giannetta,E., Gianfrilli,D.,
            Garofalo,S., Zhang,X., Chen,X., Xiang,Y.K., Lenzi,A., Pellegrini,M.
            and Naro,F.
  TITLE     Inhibition of type 5 phosphodiesterase counteracts beta2-adrenergic
            signalling in beating cardiomyocytes
  JOURNAL   Cardiovasc Res 106 (3), 408-420 (2015)
   PUBMED   25852085
REFERENCE   6  (bases 1 to 4135)
  AUTHORS   Omori,K. and Kotera,J.
  TITLE     Overview of PDEs and their regulation
  JOURNAL   Circ Res 100 (3), 309-327 (2007)
   PUBMED   17307970
  REMARK    Review article
REFERENCE   7  (bases 1 to 4135)
  AUTHORS   Vasta,V., Shimizu-Albergine,M. and Beavo,J.A.
  TITLE     Modulation of Leydig cell function by cyclic nucleotide
            phosphodiesterase 8A
  JOURNAL   Proc Natl Acad Sci U S A 103 (52), 19925-19930 (2006)
   PUBMED   17172443
  REMARK    GeneRIF: pharmacological manipulation of PDE8A, alone or in
            combination with other PDEs present in Leydig cells, may be
            exploited to modulate testosterone synthesis
REFERENCE   8  (bases 1 to 4135)
  AUTHORS   Dong,H., Osmanova,V., Epstein,P.M. and Brocke,S.
  TITLE     Phosphodiesterase 8 (PDE8) regulates chemotaxis of activated
            lymphocytes
  JOURNAL   Biochem Biophys Res Commun 345 (2), 713-719 (2006)
   PUBMED   16696947
REFERENCE   9  (bases 1 to 4135)
  AUTHORS   Glavas,N.A., Ostenson,C., Schaefer,J.B., Vasta,V. and Beavo,J.A.
  TITLE     T cell activation up-regulates cyclic nucleotide phosphodiesterases
            8A1 and 7A3
  JOURNAL   Proc Natl Acad Sci U S A 98 (11), 6319-6324 (2001)
   PUBMED   11371644
REFERENCE   10 (bases 1 to 4135)
  AUTHORS   Soderling,S.H., Bayuga,S.J. and Beavo,J.A.
  TITLE     Cloning and characterization of a cAMP-specific cyclic nucleotide
            phosphodiesterase
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8991-8996 (1998)
   PUBMED   9671792
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CO808457.1, AK148238.1, BC125578.1, AF067806.1 and AV272936.1.
            
            On Jun 5, 2018 this sequence version replaced NM_008803.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148238.1, AF067806.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-473               CO808457.1         20-492
            474-539             AK148238.1         145-210
            540-3222            BC125578.1         1-2683
            3223-4130           AF067806.1         2750-3657
            4131-4135           AV272936.1         249-253
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 45.71 cM"
     gene            1..4135
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /note="phosphodiesterase 8A"
                     /db_xref="GeneID:18584"
                     /db_xref="MGI:MGI:1277116"
     exon            1..824
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     CDS             651..3122
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /EC_number="3.1.4.53"
                     /codon_start=1
                     /product="high affinity cAMP-specific and IBMX-insensitive
                     3',5'-cyclic phosphodiesterase 8A"
                     /protein_id="NP_032829.1"
                     /db_xref="CCDS:CCDS40005.1"
                     /db_xref="GeneID:18584"
                     /db_xref="MGI:MGI:1277116"
                     /translation="MGCAPSIHTSENRTFSHSDGEDEDVDVDVPGPAPRSIQRWSTAP
                     GLVEPQPRDNGASKVSVADVQFGPMRFHQDQLQVLLVFTKEDSQCNGFHRACEKAGFK
                     CTVTKEVQTVLTCFQDKLHDIIIIDHRYPRQMDAETLCRSIRSSKFSENTVIVGVVRR
                     VDKEESSLMPFLAAGFTRRFIENPNVMACYNELLQLACGEVRSQLKLRACNSVFTALE
                     KSQEAIEITSEDHIIQYANPAFESTMGYQSGELIGKELAQVPINEKKGDLLDAINSCV
                     TVDKEWQGVYHTQKKNGDNIQQNVKIIPVIGQGGKIRHYVSIIRVCNGNNKVETTTEC
                     VQTDSQTDNQAGKHKDRRKHSMDAKAVSSRTSDVSSQRRHSSLARIHSMMIEAPITKV
                     INIINAAQENSPVPVTEALNRVLDILRTTELYSPQFNAQDDPHATDLVGGLMSDGLRR
                     FSGNEYILATKNLPPLSNNLATPVSLHDVPPRIALAIENEEQWDFDIFELEVATQNRP
                     LIYLGLKTFARFGMCEFLQCSETTLRSWFQMIESNYHSSNPYHNSTHAADVLHATAYF
                     LSRDKIKETLDRIDEVAALIAATVHDVDHPGRTNSFLCNAGNQLAVLYNDTAVLESHH
                     VALAFQLTLENDQCNIFKQMERNDYRTLRQSIIDMVLATEMTKHFEHVNKFINSINKP
                     LTAQESEEPDRSLEDIKAMLKTPESRALIKRMMIKCADVSNPCRPLEHCIEWAARISE
                     EYFSQTDEEKQLDLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPNLMQ
                     HLDDNFRYWKGLDEKKLRSLRPPPE"
     exon            825..881
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            882..1072
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1129
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1184
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1273
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1352
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1490
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1579
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1580..1631
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1674
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1752
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1823
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1985
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2034
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            2035..2170
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            2171..2369
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2587
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            2588..2717
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            2718..2885
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            2886..3015
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     exon            3016..4135
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4111..4116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /note="hexamer: ATTAAA"
     polyA_site      4133
                     /gene="Pde8a"
                     /gene_synonym="Pde8"
                     /note="major polyA site"
ORIGIN      
        1 agtctcggat gctcaactcc tggctcgcgc agccgcctcg gcgcttctcg acggcgcggc
       61 ccgaccggag tcgaggcgct cgctccgtca gcgagcgggt ccccagctcg gagtgcgggc
      121 gccgagggct gggctcccct acggtagccc gactaggggc gctcacccag ccgtccgccg
      181 ggccgaggag aggcaccgcc aggcgagcga gcgcccgttt cctcgagagt cagacgaggc
      241 gagggcggcg gtgcaaacgc ggcgcgggct cgcggctccc ggtggcgaga gcgcaaatct
      301 ccacccccga catttccccg gaagcgcggc ggctgcggcg cgcgcggcga tgtgagggag
      361 acagcggcgg ctcccgcctc aggaagggca tgttcggagg ggcgtccgcg gcgcgcctct
      421 cgccccagcg ccccctcccc acggccgccg cgcccgcagc gcaataaaag gggcggccgc
      481 gcttcctgac gcgagacccg cgcttgccgc cgccgcccac ccgcctgccc gcccgcccgc
      541 tcaggccgcg atgacacggc gcccgcggcg gccccgaggc gccgggcggg ccgtttgctg
      601 accggatcgc ggctgcccgc cagcgagtcc gcggtgccgc cgccgccagt atgggctgcg
      661 ccccgagcat ccacacttcc gaaaaccgaa cgtttagcca cagcgacggc gaggacgagg
      721 acgtggacgt ggacgtcccg ggccccgcgc cgagatctat tcagcgatgg tcgaccgcgc
      781 ccgggctcgt ggagcctcag cctcgggata acggcgcctc gaaggtatca gtagctgatg
      841 tgcaatttgg cccgatgaga tttcatcaag accaacttca ggtcctgtta gtgtttacca
      901 aagaagatag ccagtgtaac ggattccaca gggcatgtga gaaagctggg tttaagtgca
      961 cagtcaccaa ggaggtgcag actgtcctta cctgtttcca ggacaaactc catgacatca
     1021 tcatcataga ccacagatat ccccgacaga tggatgcaga gacattgtgc aggtctatca
     1081 gatcatcaaa attctcagaa aacacagtta ttgttggcgt agtacgcagg gtggacaaag
     1141 aagaatcatc tttaatgccc ttccttgctg ctggcttcac acggaggttt atagagaacc
     1201 ccaacgtcat ggcctgttat aatgaactgc tccagttggc atgtggagag gtgcgatcac
     1261 agctgaaact cagggcttgt aactcagtat tcactgcatt agagaaaagt caagaagcaa
     1321 ttgaaattac aagtgaagac cacattatac agtatgcaaa ccctgcattt gaatcaacaa
     1381 tgggttatca gtcaggagaa ttaataggga aggagttagc acaagtgccc ataaatgaaa
     1441 agaaaggtga tttgctcgat gccataaatt cgtgtgtcac agtagacaag gaatggcaag
     1501 gagtttacca tacccaaaag aaaaatggag ataacataca gcaaaatgtg aagataatac
     1561 ctgtcattgg gcagggagga aaaatcagac actatgtgtc cattatcaga gtgtgcaatg
     1621 gcaacaataa ggttgagacc acaactgagt gtgttcagac tgacagtcaa acagataacc
     1681 aggcaggcaa acataaggac aggaggaaac attcgatgga cgccaaagcg gtttcctcca
     1741 gaacaagtga cgtttccagt cagagaagac attcctcttt ggcccggata cattctatga
     1801 tgattgaggc acccatcacc aaggtaatca atattatcaa tgctgcccag gaaaacagtc
     1861 ctgtgcctgt gacggaagcc ttgaaccgag tgctggatat tctgagaacc actgagctgt
     1921 actcgccaca gtttaatgct caggatgatc cccatgccac agacctcgtt gggggcttaa
     1981 tgtctgatgg cttacgaagg ttttcaggaa atgagtatat tcttgcaaca aaaaacctcc
     2041 caccactttc aaacaatttg gccacccccg tctcccttca tgatgtccca ccacggattg
     2101 ccctggccat agaaaatgaa gaacagtggg actttgacat ttttgagctg gaagtagcaa
     2161 cccaaaatag gcctttgatt tatctaggtc tgaaaacgtt tgcgcgtttt ggaatgtgtg
     2221 agttcttaca gtgctctgag acaacgctga gatcttggtt tcagatgatc gaatccaact
     2281 accactcttc taacccctac cacaattcca cacacgctgc ggatgtgctt cacgccacgg
     2341 cgtatttcct ttccagagac aaaataaagg aaactttaga tcgcattgac gaggttgctg
     2401 ccctcattgc ggccaccgtc catgacgtgg atcaccctgg gagaaccaac tccttcctgt
     2461 gcaacgcggg aaaccagctg gctgtgctct acaacgacac cgctgtgctg gagagccacc
     2521 acgtggcact ggccttccag ctgacgcttg aaaatgacca gtgcaatatc tttaagcaaa
     2581 tggagaggaa tgactaccgg acactacgcc agagcattat tgacatggtc ctagccacag
     2641 aaatgacgaa gcactttgaa catgtcaaca aatttatcaa cagcatcaac aagcctttga
     2701 cagcccagga gagtgaggaa cctgacagaa gtctggaaga cataaaagct atgctcaaga
     2761 cgccagagag ccgggctctc atcaaaagaa tgatgattaa gtgtgccgac gtgtccaacc
     2821 cctgccggcc gctggagcac tgcatcgagt gggctgcgcg catctcagag gagtatttct
     2881 ctcagactga tgaagagaaa cagctggact tgcctgtggt gatgcccgtg tttgacagaa
     2941 acacctgcag cattcccaag tctcagatct ccttcatcga ttacttcatc acagacatgt
     3001 ttgatgcctg ggatgccttc gttgacctgc ctaaccttat gcagcaccta gatgacaact
     3061 tcaggtactg gaaaggactg gatgagaaga agcttcgaag cctccgcccc cctccagagt
     3121 agcgtgatgt acacgcctgg cctgtggctg tcctgcctgg tctctgcagt ttcttagccc
     3181 tttcactgtt agccagagct gccccctcat ctgcagagcc tttgaaagcc catgggatgc
     3241 ccaccttctg tgaccacatc tacaccatag cttgctgcaa ggctctttag tgaggccccc
     3301 aagagctgtg gtttgtatcg actctggact ctgtgttacc acagccctgg gaaccctgca
     3361 gaggtgacca tggctctcct agcagtgacc atgtgcctgc agagcagcat ctggggaggt
     3421 gtggttgcag gttgtggaac actgctgcaa gggacgtctc tcttaagtcc actagcaaac
     3481 gaggcagaca tttaaggttg attctcccga ttattacatg ttttatttat tttattagac
     3541 atttgatatt ttctatgaat ttaaaaatac ttcaaagcca atttcagatg ccttgaccaa
     3601 attcatgact tgtgtgtgcg tgatttggtt acagacctat tttcataaat gcagatttat
     3661 aagtttaaaa tgatgcattt ttcccacact atggaatatg ttaaaccctc ctctttgatg
     3721 ttagtgggga aggctttatg tagtgggtct cactctagtg tgtaacagag aagccacgtc
     3781 tgcagacaag ctctgagcag ctgctgtcag gagttttaca ggtgccttct cagagaaaaa
     3841 ttggatttga ccattttttt tccctaaaat tttataattg aatttctaaa agcctgcctc
     3901 attttatacc atttctataa aatatgccct gcaaagcaaa cctgggtacc tagcagctga
     3961 tattttacaa ctaacatggg gtatttgagt ctagaaatat aaagggtcta tctcctcaca
     4021 ttttgaataa gcataatact ataaagatga tattcaatga catgtcgaaa tgttagtttc
     4081 attttaaact tgtatttttt tctggatgaa attaaactat ttgtttttaa gaagt
//
