LOCUS       NM_153422               6713 bp    mRNA    linear   ROD 14-OCT-2024
DEFINITION  Mus musculus phosphodiesterase 5A, cGMP-specific (Pde5a),
            transcript variant 1, mRNA.
ACCESSION   NM_153422 XM_001001000
VERSION     NM_153422.3
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Li,S., Xin,Q., Yan,Y., Wang,X., Ai,H., Que,B., Gong,W. and Nie,S.
  TITLE     Pde5 Inhibition Reduced Blood Pressure and Alleviated Target Organ
            Damage in Chronic Intermittent Hypoxia
  JOURNAL   J Cardiovasc Pharmacol 84 (1), 81-91 (2024)
   PUBMED   38030140
  REMARK    GeneRIF: Pde5 Inhibition Reduced Blood Pressure and Alleviated
            Target Organ Damage in Chronic Intermittent Hypoxia.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Lippner,D.S., Xu,J., Ma,S., Reisert,J. and Zhao,H.
  TITLE     Phosphodiesterase 5A regulates the vomeronasal pump in mice
  JOURNAL   Genesis 62 (3), e23603 (2024)
   PUBMED   38738564
  REMARK    GeneRIF: Phosphodiesterase 5A regulates the vomeronasal pump in
            mice.
REFERENCE   3  (bases 1 to 6713)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 6713)
  AUTHORS   Giorgi,M., Miele,A.E., Cardarelli,S., Giorgi,A., Massimi,M.,
            Biagioni,S. and Saliola,M.
  TITLE     Structural Characterization of Murine Phosphodiesterase 5 Isoforms
            and Involvement of Cysteine Residues in Supramolecular Assembly
  JOURNAL   Int J Mol Sci 24 (2), 1108 (2023)
   PUBMED   36674621
  REMARK    GeneRIF: Structural Characterization of Murine Phosphodiesterase 5
            Isoforms and Involvement of Cysteine Residues in Supramolecular
            Assembly.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6713)
  AUTHORS   Zou,T., Wang,T., Zhen,F., He,X., Dong,S. and Zhang,H.
  TITLE     Exogenous PDE5 Expression Rescues Photoreceptors in RD1 Mice
  JOURNAL   Curr Med Chem 29 (40), 6115-6124 (2022)
   PUBMED   35170405
  REMARK    GeneRIF: Exogenous PDE5 Expression Rescues Photoreceptors in RD1
            Mice.
REFERENCE   6  (bases 1 to 6713)
  AUTHORS   Das,A., Xi,L. and Kukreja,R.C.
  TITLE     Phosphodiesterase-5 inhibitor sildenafil preconditions adult
            cardiac myocytes against necrosis and apoptosis. Essential role of
            nitric oxide signaling
  JOURNAL   J Biol Chem 280 (13), 12944-12955 (2005)
   PUBMED   15668244
  REMARK    GeneRIF: sildenafil inhibits PDE5 and preconditions adult cardiac
            myocytes against necrosis and apoptosis
REFERENCE   7  (bases 1 to 6713)
  AUTHORS   Takimoto,E., Champion,H.C., Li,M., Belardi,D., Ren,S.,
            Rodriguez,E.R., Bedja,D., Gabrielson,K.L., Wang,Y. and Kass,D.A.
  TITLE     Chronic inhibition of cyclic GMP phosphodiesterase 5A prevents and
            reverses cardiac hypertrophy
  JOURNAL   Nat Med 11 (2), 214-222 (2005)
   PUBMED   15665834
  REMARK    GeneRIF: findings show a much greater role of PDE5A in hearts
            subjected to sustained pressure load and that PDE5A inhibition in
            this setting prevents and reverses cardiac chamber, cellular and
            molecular remodeling induced by this stimulus
REFERENCE   8  (bases 1 to 6713)
  AUTHORS   Takimoto,E., Champion,H.C., Belardi,D., Moslehi,J., Mongillo,M.,
            Mergia,E., Montrose,D.C., Isoda,T., Aufiero,K., Zaccolo,M.,
            Dostmann,W.R., Smith,C.J. and Kass,D.A.
  TITLE     cGMP catabolism by phosphodiesterase 5A regulates cardiac
            adrenergic stimulation by NOS3-dependent mechanism
  JOURNAL   Circ Res 96 (1), 100-109 (2005)
   PUBMED   15576651
  REMARK    GeneRIF: PDE5A has a novel regulatory role in hearts under
            adrenergic stimulation. PDE5A catabolic regulation is specific
            coupled with NOS3-derived cGMP attributable to protein subcellular
            localization and targeted synthetic/catabolic coupling.
REFERENCE   9  (bases 1 to 6713)
  AUTHORS   Rybalkin,S.D., Rybalkina,I.G., Shimizu-Albergine,M., Tang,X.B. and
            Beavo,J.A.
  TITLE     PDE5 is converted to an activated state upon cGMP binding to the
            GAF A domain
  JOURNAL   EMBO J 22 (3), 469-478 (2003)
   PUBMED   12554648
  REMARK    GeneRIF: Data show that recombinant cyclic GMP-binding
            phosphodiesterase 5 (PDE5) is activated directly upon cGMP binding
            to the GAF A domain, and this effect does not require PDE5
            phosphorylation.
REFERENCE   10 (bases 1 to 6713)
  AUTHORS   Loughney,K., Hill,T.R., Florio,V.A., Uher,L., Rosman,G.J.,
            Wolda,S.L., Jones,B.A., Howard,M.L., McAllister-Lucas,L.M.,
            Sonnenburg,W.K., Francis,S.H., Corbin,J.D., Beavo,J.A. and
            Ferguson,K.
  TITLE     Isolation and characterization of cDNAs encoding PDE5A, a human
            cGMP-binding, cGMP-specific 3',5'-cyclic nucleotide
            phosphodiesterase
  JOURNAL   Gene 216 (1), 139-147 (1998)
   PUBMED   9714779
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC101922.12 and AC155158.15.
            
            On Jul 22, 2022 this sequence version replaced NM_153422.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF541937.1, BC119137.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-195               AC101922.12        88958-89152         c
            196-784             AC101922.12        69848-70436         c
            785-874             AC101922.12        57358-57447         c
            875-946             AC101922.12        43701-43772         c
            947-1036            AC101922.12        41980-42069         c
            1037-1174           AC101922.12        39253-39390         c
            1175-1242           AC101922.12        36691-36758         c
            1243-1351           AC101922.12        23957-24065         c
            1352-1439           AC101922.12        23004-23091         c
            1440-1615           AC101922.12        15118-15293         c
            1616-1675           AC101922.12        13716-13775         c
            1676-1822           AC101922.12        258-404             c
            1823-1948           AC155158.15        147020-147145       c
            1949-2043           AC155158.15        145192-145286       c
            2044-2131           AC155158.15        134561-134648       c
            2132-2232           AC155158.15        130559-130659       c
            2233-2310           AC155158.15        129153-129230       c
            2311-2374           AC155158.15        127093-127156       c
            2375-2449           AC155158.15        118477-118551       c
            2450-2533           AC155158.15        117580-117663       c
            2534-6713           AC155158.15        110827-115006       c
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 53.69 cM"
     gene            1..6713
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /note="phosphodiesterase 5A, cGMP-specific"
                     /db_xref="GeneID:242202"
                     /db_xref="MGI:MGI:2651499"
     exon            1..195
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            196..784
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            785..874
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            875..946
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1036
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1174
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1242
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1351
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1439
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1615
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1675
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1676..1822
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1948
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2043
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2044..2131
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2232
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2233..2310
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2311..2374
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2449
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2450..2533
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     exon            2534..6713
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6693..6698
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /note="hexamer: AATAAA"
     polyA_site      6713
                     /gene="Pde5a"
                     /gene_synonym="Cgbpde; Cn5n; Pde5; Pde5a1; PDE5a2"
                     /note="major polyA site"
ORIGIN      
        1 actgtcctgc tcctctgaga gagagaggtg gaaatccggc acctccgagc cgcagagacg
       61 cgcggcaagc accatggaac gagcgggccc caactccgtg cggtcgcagc agcagcggga
      121 cccggactgg gtggaagcgt ggctggatga tcaccgggac tttaccttct cttactttat
      181 tagaaaggcc accagagaca tggtcaacgc atggttttca gagagagttc acaacatccc
      241 tgtgtgcaag gaaggcatcc gagcccatac cgagtcctgc tcttgctctc tgcagcaaag
      301 tcctcatgca gataatacca cccctggagc accagccaga aaaatatctg cctctgaatt
      361 tgaccgacct cttagaccca ttgttgtcaa ggattccgag ggaacagtga gctttctctc
      421 tgactcagga aaaaaggaac aaatgccact aaccccccca agatttgata gtgatgaagg
      481 ggaccagtgc tcaaggctct tggagctagt aaaagatatt tccagtcatt tggatgtcac
      541 agcattgtgt cacaaaattt tcttgcatat ccatggactc atctctgcgg atcgctattc
      601 cctgttcctt gtctgtgagg acagctctaa agacaaattc ctcatcagcc gcctctttga
      661 tgttgctgaa ggttcaacat tggaggaagc ttcaaataac tgtatccgtt tagagtggaa
      721 caaaggcatt gtgggacatg tggcagcttt tggcgagccc ttgaacatca aagatgccta
      781 tgaggatccc cggttcaatg cagaagttga ccaaattaca ggctacaaga cacaaagtat
      841 cctttgtatg ccaattaaga accacaggga agaggttgtt ggtgtagctc aggccatcaa
      901 caagaaatca ggaaatggtg ggaccttcac tgaaaaagat gaaaaggact ttgcggccta
      961 cctggcattc tgtggtattg ttcttcacaa tgctcagctc tatgaaacgt cattgctgga
     1021 gaacaaaaga aatcaggtgt tacttgacct tgccagctta atctttgaag agcagcagtc
     1081 actggaagtc attctgaaga agatagcggc cactatcatc tccttcatgc aggtgcagaa
     1141 gtgcaccatc ttcattgtgg atgaagactg tcctgactct ttctctcgtg tgtttcacat
     1201 ggagtgtgag gaagtaggaa aaccctctga tcctttaacg agggagcaag atgcaaacaa
     1261 aatcaactac atgtatgctc agtatgtcaa aaacacgatg gagccactta atatcccaga
     1321 tgtcaccaag gacaaaagat ttccctggac aaatgaaaac atgggacacg tgaacacgcc
     1381 ctgcattgga agtttgcttt gcacccctat aaaaaatggg aagaagaaca aagtcatagg
     1441 cgtctgccaa cttgttaata agatggagga gaatactggc aagattaaag ctttcaacca
     1501 aaatgatgag cagttcctgg aagcctttgt catcttctgt ggcttgggga tccagaacac
     1561 acagatgtat gaagcggtgg agagagccat ggccaagcag atggtgacat tagaggtcct
     1621 gtcttaccat gcatcagcgg cggaggaaga aacaagagag ctccaggctt tatcggctgc
     1681 tgtggtacca tcggcccaaa cccttaaaat taccgacttc agcttcagtg actttgagct
     1741 gtctgatctg gaaacagcgc tgtgtacaat tcggatgttc actgacctca accttgtgca
     1801 gaacttccag atgaaacatg aggttctttg caggtggatt ttaagtgtga agaagaatta
     1861 tcggaagaac gttgcctacc acaattggag gcatgccttt aacacggcac agtgcatgtt
     1921 tgctgctctg aaagccggca agatccagaa caagttgacg gatctggaga cccttgcgtt
     1981 gctcattgct gctttaagcc atgacttgga tcatcgtggc gtgaacaact catacataca
     2041 gcggagcgag cacccgctgg ctcagctgta ctgtcactcc atcatggaac accatcattt
     2101 tgaccagtgc ttgatgattc taaacagccc aggcaaccag attctcagtg gcctctccat
     2161 tgacgaatat aagaccacac tgaaaataat caagcaagca attttagcca cagacctagc
     2221 actgtacatt aagagacgag gagaattttt tgaacttata cgaaaaaatc aattcagttt
     2281 tgaagatcct cttcaaaagg agttgtttct agcaatgctg atgacagcct gcgacctctc
     2341 tgcgattact aaaccctggc ctattcaaca acggatagca gaactcgtgg cagctgaatt
     2401 ctttgatcaa ggagacagag agagaaaaga acttaacatg gagccggctg atctaatgaa
     2461 cagagagaag aaaaataaaa tcccaagtat gcaagttggg ttcatagatg ccatctgctt
     2521 gcagctgtat gaggccctga cccacgtatc cgaagactgt ttgcccttgc tggatggctg
     2581 caggaagaac agacagaaat ggcaggccct cgcggagcag caggagaaga tgctccttaa
     2641 cggggagagc agccagggca agcgggactg actgatgcct ccctcagccc cgcccccaca
     2701 cccctccccg tgtacaaact gccacaggcc tggtttccca gtccactatg cagacttgag
     2761 tgcactttgc cactgctgta ttttattttt tgcacaactt tggagagcgt gaagtgtttc
     2821 agagattgct acctactttc catccttttg ttttatgcca ctgaagtgga gcgatcaagt
     2881 catgcccact ctaacgcttt tgataaggat ggtgtaaacg gcgattctac actcagcttg
     2941 gtcatgctcg gggattataa atactggcct ttgtgttcct gagtcaccac ttttttaaaa
     3001 caccttagga atggttctga aaagtgggtt aggggcaaag taaatgtgtt cggggacatt
     3061 gaaagccacc ttgaaaatta atgctgaccg agtgggtgtt agggagaaat taatataaac
     3121 ttgtcagaag tgtcttaacc aagttaaaga attgggaaac aagaaaacgt ggtacctaat
     3181 tgctctgctc tttctaggat ggggagaact tgcagtatat cccatctacg tggtagaaca
     3241 aacttaagct gcaaaccatc catgcagccg ccctcttcag caagcagact ccccactgcg
     3301 tctaaacccc aggccagatg attcccctcc cccagctgtc ccaagagaga catgggaggg
     3361 ccctggatag ccctccacca agagaaatgt tttacttgaa gatctttctg acaatccaca
     3421 tttttaaata ctgaaaactt ttaaaactaa ttgctttttt gggggggagg ggtcgttgtt
     3481 tttttttttc gagacagggt ttctctgtgt agccctggct gtcctggaac tcactctgta
     3541 atcccagcac tcaggaggca gaggcaggag gatttctgag ttcgaggcca gtctggtcta
     3601 caaagtgagt tccaggacaa ccagggctac acagagaaac tctgtctcaa aaaacaaaca
     3661 aacaaacaaa caaacaaaca aaaagccaac taattttttt ttttaatttt tcttttgctt
     3721 ttcatctcct taatccaaca gtttaagctt caacagagaa acggtcacag gggcacatgc
     3781 ctgaaggcag atatgactac agatacgcga gtgcaagttg agtctttatc ccttctgtca
     3841 actgaaattg acaggaaaaa aatattaata attctatgag tagcagaggt ttgtctccaa
     3901 taggcaggtc agacaatgaa ttcaaaggaa cagtctgtga acgtcttcct gcccaaacaa
     3961 aacagcattc agtattagaa gtagaaagtg agtcaaaaaa caaaacaaaa caaaacaaca
     4021 aacaaaaaca aacaaacaaa aaaaccaaat aactgcatta ggggaggagc atctgttcca
     4081 gatgtgcaga tctgttccag atgtgcagaa aggtttgggt ttgggcagaa ttagaaggat
     4141 ttgatttatt cattcccacc ctttccccac atcctcccaa agagggtaat gtattttgtc
     4201 tgggtttcta gcattaattt gcattaaaat ggtttctctc ttgcttttct ctctgtttaa
     4261 aattcaacat ggtcatttta atcctgtgta tgtatattgt attgtggctt tgttcttcct
     4321 cagatatgaa aaggcaaagc aggctatact ggcagtgttt tgtattggtc aaggctagac
     4381 cagggaattg tcacccaata ctaaatgtat tcttgttgta atacttgtat ttttttttca
     4441 gaattttatc ttatttttat ttcatgtcat agagatgaaa cctggaaggt tgctctaacg
     4501 cactggtgtg taaatggtac agactgtcac taatagtaat cacaccagcc tcacactcgc
     4561 ctatgcagag tgtaggcgca ctgcttagag gacattctac aagcctggca ctgtgaccga
     4621 gcagctatgc tgaaatgcag tcatatacat gtgttgcttc tccacactcc tctgacctaa
     4681 gtcactgaga atattcttaa agagttcatt atatgtggag ggtttgtcta acatagtgac
     4741 aggcaagaag ccgtatgtac attgaaaatc attcggtttg ctttaggtcg tggtttgtct
     4801 tactctacat attaacatag gaaaagcact gtgatatttt attgctgggt attttattca
     4861 aataattttt attttatagg aaaaccattc catacatgga atgctatctg tggtacaaga
     4921 acacaaatca tagtttttgt tctgaatgtg aatattgcct ttctagtgtc cactcaagtt
     4981 ggaatatatc tatcacatta tattatcatt cctctctcat gctgccgtga atccatagtg
     5041 cccgtaatga acaagaaagt gttcttgtcc tggactgttc tcttgacaaa tttcttggct
     5101 ggctatagtg aaataatatg atatcatgtc atttttaact ttcagccaga aaaatcctcg
     5161 tggtttggta agttgtctat aaagttttct gatcttacac gtttagaaga gttagctgcc
     5221 tttaccagtc agggtactca cgtagtttgg tttagacaaa gcctgtgttt aaggggcatg
     5281 ccccccaaga aatagctagc tcttaagctt ttttatggac ctttgagacc actgttgcaa
     5341 aatggggtat tgaggtaatg acctgacagc gcccgtcatt tcttcgctga gttccttaga
     5401 tggaaaacaa gtattgttaa ttcactccac acagacctgg ctcggagcaa aaatgtagga
     5461 tgatactctg tcactggttg gctaattaca aagaagaact tcttttagca gcaacacgga
     5521 gacaagagta tatatgaact tgagtcagaa attcttgcct accagttaag tgcctttact
     5581 ctcagaacgc tgccgaagtg taaggcttgg cacctatcac atttccaaat ttcctgtgtt
     5641 tctcactaga ttacactcct aaaccttatc aggtaagcgg catggccttt tgctttaact
     5701 tgatttaaga tgagaagaga tgaaatgttc cccgctctcc tgtaaacagc tgtctcaaat
     5761 aaggtggcag aatttaatga ctcagtgtca accatgcatc agcccgtttc ttattaaatc
     5821 ctctccgtat gttaggcaca gtgctcagat gctgagacac agcaggaagt gccaggagct
     5881 catgggccaa gcatggaatg agtaagggca tcgcttctca tcccgccaac tcaatgtgca
     5941 agtatatagg gctttccacc actttcccct tcttctgtaa catacataac atgcactttt
     6001 atccaaaatt tcttaagaat ctgtcagtga acattccggc ttcctgttgg cgtggaccat
     6061 ttaccttgct gatagcttaa agagcacctt acgcaaggag tcacatcttc cacaacatag
     6121 gtcatgtttc catattgcaa ctagagagag agagagagat cggagttctc agactattca
     6181 actgcagatt tggactcttt ctgccctctc acagagagca aagaaggaag tattaaagct
     6241 tacctcaccc ttccacattg ttatcaaaag cctgtgtaga catgtcttga atgtcattga
     6301 aagtgatgta aatagctgaa aaatataaat ttctattacc gtttaagaac tctagtgaac
     6361 cctgattcgc cactgttgat gacctatttg tattcttatg ccttttcaat atatctgaac
     6421 aaatatatag tttctggcac tatttttata ctagcgtaaa agagttacta tgtaaattat
     6481 gttttgttgt gaagtcatct aaaaatggct gaaatgtcgg tttctttcct ttcctcaccc
     6541 cacattataa accatttcca gtgatccttc tgtaagaatg tgactctgca gaggcacaca
     6601 cccacctgcc tcgggtgtct gtggagtcag acctggtcag ggtacttgtg aagtcaccac
     6661 gttgtcagtt gtcatttttg cagtgctgta aaaataaagt gaatgaacga gaa
//
