LOCUS       NM_183408               4630 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus phosphodiesterase 4A, cAMP specific (Pde4a),
            transcript variant 5, mRNA.
ACCESSION   NM_183408
VERSION     NM_183408.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Elnagdy,M., Wang,Y., Rodriguez,W., Zhang,J., Bauer,P., Wilkey,D.W.,
            Merchant,M., Pan,J., Farooqui,Z., Cannon,R., Rai,S., Maldonado,C.,
            Barve,S., McClain,C.J. and Gobejishvili,L.
  TITLE     Increased expression of phosphodiesterase 4 in activated hepatic
            stellate cells promotes cytoskeleton remodeling and cell migration
  JOURNAL   J Pathol 261 (3), 361-371 (2023)
   PUBMED   37735782
  REMARK    GeneRIF: Increased expression of phosphodiesterase 4 in activated
            hepatic stellate cells promotes cytoskeleton remodeling and cell
            migration.
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Tibbo,A.J., Mika,D., Dobi,S., Ling,J., McFall,A., Tejeda,G.S.,
            Blair,C., MacLeod,R., MacQuaide,N., Gok,C., Fuller,W., Smith,B.O.,
            Smith,G.L., Vandecasteele,G., Brand,T. and Baillie,G.S.
  TITLE     Phosphodiesterase type 4 anchoring regulates cAMP signaling to
            Popeye domain-containing proteins
  JOURNAL   J Mol Cell Cardiol 165, 86-102 (2022)
   PUBMED   34999055
  REMARK    GeneRIF: Phosphodiesterase type 4 anchoring regulates cAMP
            signaling to Popeye domain-containing proteins.
REFERENCE   3  (bases 1 to 4630)
  AUTHORS   Boyd,A., Aragon,I.V., Rich,J., McDonough,W., Oditt,M., Irelan,D.,
            Fiedler,E., Abou Saleh,L. and Richter,W.
  TITLE     Assessment of PDE4 Inhibitor-Induced Hypothermia as a Correlate of
            Nausea in Mice
  JOURNAL   Biology (Basel) 10 (12), 1355 (2021)
   PUBMED   34943270
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4630)
  AUTHORS   Boyd,A., Aragon,I.V., Abou Saleh,L., Southers,D. and Richter,W.
  TITLE     The cAMP-phosphodiesterase 4 (PDE4) controls beta-adrenoceptor- and
            CFTR-dependent saliva secretion in mice
  JOURNAL   Biochem J 478 (10), 1891-1906 (2021)
   PUBMED   33944911
REFERENCE   5  (bases 1 to 4630)
  AUTHORS   Aragon,I.V., Boyd,A., Abou Saleh,L., Rich,J., McDonough,W.,
            Koloteva,A. and Richter,W.
  TITLE     Inhibition of cAMP-phosphodiesterase 4 (PDE4) potentiates the
            anesthetic effects of Isoflurane in mice
  JOURNAL   Biochem Pharmacol 186, 114477 (2021)
   PUBMED   33609559
REFERENCE   6  (bases 1 to 4630)
  AUTHORS   Cherry,J.A. and Davis,R.L.
  TITLE     Cyclic AMP phosphodiesterases are localized in regions of the mouse
            brain associated with reinforcement, movement, and affect
  JOURNAL   J Comp Neurol 407 (2), 287-301 (1999)
   PUBMED   10213096
REFERENCE   7  (bases 1 to 4630)
  AUTHORS   Sullivan,M., Rena,G., Begg,F., Gordon,L., Olsen,A.S. and
            Houslay,M.D.
  TITLE     Identification and characterization of the human homologue of the
            short PDE4A cAMP-specific phosphodiesterase RD1 (PDE4A1) by
            analysis of the human HSPDE4A gene locus located at chromosome
            19p13.2
  JOURNAL   Biochem J 333 (Pt 3) (Pt 3), 693-703 (1998)
   PUBMED   9677330
REFERENCE   8  (bases 1 to 4630)
  AUTHORS   MacKenzie,S.J., Yarwood,S.J., Peden,A.H., Bolger,G.B., Vernon,R.G.
            and Houslay,M.D.
  TITLE     Stimulation of p70S6 kinase via a growth hormone-controlled
            phosphatidylinositol 3-kinase pathway leads to the activation of a
            PDE4A cyclic AMP-specific phosphodiesterase in 3T3-F442A
            preadipocytes
  JOURNAL   Proc Natl Acad Sci U S A 95 (7), 3549-3554 (1998)
   PUBMED   9520403
REFERENCE   9  (bases 1 to 4630)
  AUTHORS   Cherry,J.A. and Davis,R.L.
  TITLE     A mouse homolog of dunce, a gene important for learning and memory
            in Drosophila, is preferentially expressed in olfactory receptor
            neurons
  JOURNAL   J Neurobiol 28 (1), 102-113 (1995)
   PUBMED   8586960
REFERENCE   10 (bases 1 to 4630)
  AUTHORS   Milatovich,A., Bolger,G., Michaeli,T. and Francke,U.
  TITLE     Chromosome localizations of genes for five cAMP-specific
            phosphodiesterases in man and mouse
  JOURNAL   Somat Cell Mol Genet 20 (2), 75-86 (1994)
   PUBMED   8009369
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163637.4.
            
            On Jul 13, 2022 this sequence version replaced NM_183408.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC139408.1, AK153657.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-496               AC163637.4         100943-101438       c
            497-688             AC163637.4         75657-75848         c
            689-725             AC163637.4         74744-74780         c
            726-796             AC163637.4         74585-74655         c
            797-846             AC163637.4         72551-72600         c
            847-959             AC163637.4         72317-72429         c
            960-1053            AC163637.4         68489-68582         c
            1054-1250           AC163637.4         65758-65954         c
            1251-1349           AC163637.4         63879-63977         c
            1350-1514           AC163637.4         63623-63787         c
            1515-1614           AC163637.4         62752-62851         c
            1615-1769           AC163637.4         62051-62205         c
            1770-1892           AC163637.4         61762-61884         c
            1893-2075           AC163637.4         60848-61030         c
            2076-4630           AC163637.4         53927-56481         c
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 7.73 cM"
     gene            1..4630
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /note="phosphodiesterase 4A, cAMP specific"
                     /db_xref="GeneID:18577"
                     /db_xref="MGI:MGI:99558"
     exon            1..496
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..2726
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /EC_number="3.1.4.53"
                     /note="isoform 5 is encoded by transcript variant 5;
                     cAMP-specific 3',5'-cyclic phosphodiesterase 4A; dunce;
                     cyclic AMP specific phosphodiesterase PDE4A5A;
                     cAMP-specific phosphodiesterase PDE4A; cAMP-specific
                     phosphodiesterase 4A; 3',5'-cyclic-AMP phosphodiesterase
                     4A"
                     /codon_start=1
                     /product="3',5'-cyclic-AMP phosphodiesterase 4A isoform 5"
                     /protein_id="NP_899668.1"
                     /db_xref="CCDS:CCDS22895.1"
                     /db_xref="GeneID:18577"
                     /db_xref="MGI:MGI:99558"
                     /translation="MEPPAAPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQ
                     QRGYSDSAERSEPERSPHRPIERADAVDTGDRPGLRTTRMSWPSSFHGTGTGGGSSRR
                     LEAENGPTPSPGRSPLDSQASPGLMLHAGAATSQRRESFLYRSDSDYDMSPKTMSRNS
                     SVASEAHGEDLIVTPFAQVLASLRNVRSNFSLLTNVPIPSNKRSPLGGPPSVCKATLS
                     EETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGN
                     QVSEYISNTFLDKQHEVEIPSPTPRQRPFQQPPPAAVQQAQPMSQITGLKKLVHTGSL
                     NINVPRFGVKTDQEDLLAQELENLSKWGLNIFCVSEYAGGRSLSCIMYTIFQERDLLK
                     KFHIPVDTMMTYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEIL
                     AALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEENCDIF
                     QNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRI
                     QVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASV
                     EKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNRDWYHSAIRQSPSPTLEEEPG
                     VLSDPALPDKFQFELTLEEEDEEDSLEVPGLPCTEETLLAPHDTRAQAMEQSKVKGQS
                     PAVVEVAESLKQETASAHGAPEESAEAVGHSFSLETSILPDLRTLSPSEEAQGLLGLP
                     SMAAEVEAPRDHLAAMRACSACSGTSGDNSAVISAPGRWGSGGDPA"
     exon            497..688
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            689..725
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            726..796
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            797..846
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            847..959
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1053
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1250
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1349
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1514
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1614
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            1615..1769
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            1770..1892
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2075
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..4630
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4185..4190
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /note="hexamer: TATAAA"
     polyA_site      4209
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
     regulatory      4579..4584
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /note="hexamer: ATTAAA"
     polyA_site      4630
                     /gene="Pde4a"
                     /gene_synonym="D9Ertd60e; Dpde2"
                     /note="major polyA site"
ORIGIN      
        1 gcagcgggct ccaagcgcca ggaggacacc ggcgccgccg ccgtccgcgt tctccgtcct
       61 tgggcgccat ggccccactg aggccaggag ctcccgtggg tccccaagca cctcggccgt
      121 ggcgccactg agctcggaca ggccagggga cgtttttgcc cccgggcacc ccacaagaca
      181 gagcgggagc catggagcct ccggccgccc cctcggagag gagcctgtct ctctctcttc
      241 ccgggccccg agagggccag gccaccctga agccgccccc ccagcacctg tggcggcagc
      301 cgaggacccc gatccgcatc cagcagcgcg gctactcgga cagtgccgag cgctcagagc
      361 cagagcgctc gccgcaccgg cccatagagc gcgccgacgc cgtggacact ggcgaccggc
      421 caggcctgcg gactacccgc atgtcctggc cctcgtcctt ccacggcacc ggtaccggcg
      481 gaggcagcag taggcgcttg gaggcagaaa atgggccaac accgtcccct ggccgcagcc
      541 ccctggactc gcaggcgagc ccggggctta tgctgcatgc cggggcagcc accagccagc
      601 gccgagaatc cttcctctat cgctcagaca gcgactatga catgtcaccg aagactatgt
      661 ccaggaactc atccgtcgcc agcgaagcgc atggtgaaga cctcattgtg accccgtttg
      721 cccaggtgct ggccagtctc cgcaacgttc gaagcaactt ctcactccta accaatgtgc
      781 ccatccccag caacaagagg tccccgctgg gcgggccacc ctctgtctgc aaggccacac
      841 tgtcagagga gacatgccag cagctggccc gggagaccct ggaagagctg gattggtgcc
      901 tggagcagct ggagaccatg cagacctacc gctctgtcag cgagatggcc tcacacaagt
      961 ttaaaaggat gctgaaccgg gaactcacac acctgtcgga aatgagcagg tcaggaaacc
     1021 aggtctcaga gtacatttcc aacacgttcc tagacaagca gcacgaagtg gagatcccat
     1081 cgcccacgcc gcgacagaga cccttccagc agcccccacc agcagcagtg cagcaggccc
     1141 agcccatgtc tcagatcaca gggctgaaaa agctggtaca caccggaagc ttgaacatca
     1201 atgtcccacg atttggagtc aagacagatc aggaggacct cttagcacaa gaactggaga
     1261 acttgagcaa atggggcctg aacatctttt gtgtgtcgga gtacgctgga ggccgttcac
     1321 tcagctgtat catgtatacg atattccagg agcgggactt actgaagaaa ttccacatcc
     1381 ccgtggacac catgatgaca tacatgctga cgctggagga ccactaccac gctgacgtgg
     1441 cctaccacaa cagcctgcac gcagccgatg tgctacagtc cacacatgtg ctgctggcca
     1501 cgcctgcact ggatgccgtg ttcacagacc tggagattct tgctgctctc ttcgctgctg
     1561 ccatccatga cgtggaccac cctggcgtct ccaaccagtt cctaatcaac accaattcgg
     1621 agctggcatt gatgtacaac gatgagtctg tgcttgagaa ccaccacctg gctgtgggat
     1681 tcaagctgct gcaagaagag aactgcgaca tcttccagaa cctcagcaag cgccagcggc
     1741 agagcctgcg caagatggtc atcgacatgg tgctggccac agacatgtcc aagcacatga
     1801 cccttctggc tgacctgaag actatggtag agacgaagaa agtgaccagc tctggagttc
     1861 tcttgctgga caactactct gaccgtatcc aggtcctcag gaacatggtg cactgtgcag
     1921 acctcagcaa tcccaccaag cccctggagc tgtaccgaca atggactgat cgcatcatgg
     1981 ctgagttctt ccagcagggt gaccgagaac gggaacgcgg aatggagatt agccccatgt
     2041 gtgacaagca cacagcctct gtggagaagt ctcaggtggg cttcatcgac tatattgttc
     2101 acccactgtg ggagacgtgg gccgatctcg tccaccccga tgcccaagac atcctggaca
     2161 cgctggaaga caaccgggat tggtatcaca gtgccatccg ccagagtccc tccccaaccc
     2221 tggaagagga gccaggagtg ctcagcgatc cggccctgcc tgacaagttc caatttgagc
     2281 tcaccttgga ggaagaggat gaagaggatt cattggaggt tccaggattg ccttgcactg
     2341 aggaaactct cctggctcca catgatacca gagctcaagc catggaacag tcaaaggtca
     2401 aaggccagag ccctgctgtt gtggaggtag cagagagttt gaagcaggag acagcctcag
     2461 cacatggcgc tcctgaggag tccgcggagg ctgtgggcca ttccttcagc cttgaaacct
     2521 ctattctgcc tgacttgagg accctgtccc cctcagagga ggcccagggc ctcctgggcc
     2581 tcccctccat ggcggcagag gtggaggccc caagagacca tctggctgcc atgagggctt
     2641 gttctgcctg ctcggggaca tcaggagaca attctgccgt catctctgct ccaggcaggt
     2701 gggggtcagg tggagaccct gcctgatccc ttttcccttc accctcggct cctccctcct
     2761 ccccacactc tccactcacc acagaccacc tcaacgactc ctggccctcc tgagaaaaaa
     2821 gaaaacagaa aagtggggtt ttttctcttt tctttttttc acccttcccc ccctgccctt
     2881 gcccacaggg cctttttgtt gaagtggggt atggagagcc aggaactgaa gtcccccaaa
     2941 aaagggattt tatttttttg aattttaatt gtaacgtttt tagaaaaaga acaaaaagaa
     3001 aaaaaaaaaa agagtgaaac acatcagctg tagatgctct ccctcccggc ccctgggccc
     3061 acctcgttcc ctcatcaccc tacctatcaa cctcagttcc aaggcgccat cctcctgcct
     3121 cttttgagac ttgctccttg agattcaagc ctaggtgtgg cactgccctt tggtcagtcc
     3181 ctccatacgg agggagtcta caacccgatt gggaatagcc accgggagag gctgtcacag
     3241 gagaggctgg cccacagcag gcctctccct ggctcactgt cctctaccac aaagtcttcc
     3301 atgtctctcc tctctgtttt ctatctttgt ttgttcaact ctggccacat ggtgaaagag
     3361 gtggtcagac gggctgttgc tttatgatcc agggactctt ggtttcaccc cgaccaccag
     3421 cctcagcctg gggtcccaag tctcaattcc cttctctgga tgccatcttc ctggtccact
     3481 ctgggtcact ttgaattctc tccgttggag gaagggggct ctgttccatg cgcacaaaca
     3541 catcctcctt ccagctgctt cttcctcttg tttctgcctt aacgattcct gtggccatag
     3601 gtgaagtctc catggctccc tcctgctccc tgggtcctat ctcccagcat agatgacact
     3661 cctttgccag catccactca accacccttc agccccgtca ctccactccc ctacaccaac
     3721 tctcattgcc ctaggaggaa gcaataatgg tggtaccaca tcctcccatc ctgggcagcc
     3781 ctcccccccc atcttactga gcctgacccc ccacccccag ccacaggcag tccctaagca
     3841 atacagatag agcaaggcca ttttctccaa gccatggggg tgaggggact tttaaaaagg
     3901 aatgagccca tttccccctg ccacatggaa ccagtctgca gcagggcagt gggggctgtc
     3961 attcctcgcc tgccctgtta catctctgta cacggtactg tagcctccac tccaggctcc
     4021 ccagatatcc ctgtctaacc cattaaccca ctccccaccc ccgatgctta cagggttttg
     4081 atttatttgt gtattaagca agagtagttt ctgacctagc tggtgatgag aatgggccac
     4141 cctcctttcc atgtgtgggt tttgtacata ctgtaaggtt gatctataaa ttattctact
     4201 gagacaaaag gagacagtga acttgttttt ccccggttga ccccgtccca ttgggaaggc
     4261 gaggaggggt tctccgggag agtagcacct ctctgctgta tcatacaagc tgtaccatat
     4321 tccctgaatg tagggtgggc atgctgtcgg tttgtgggaa gttgtgtcat aactgaggag
     4381 ggttggaggt ggcttgccct gttctcttcc ttctccaaga gcctaagata gggtccgagg
     4441 actgaccaga cccctggcct cagtcggatc ctcccgccag ggtgggtgta cacgtgagct
     4501 gcctatggtg tgttgtccac actgacagtt gatcaattgg tccgtcgatt gacaatcagt
     4561 cctccagtct tgtccctgat taaagcgagg ctgtcaccgg tgctcccggc tgatggctga
     4621 tatggcttca
//
