LOCUS       NM_018779              10794 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus phosphodiesterase 3A, cGMP inhibited (Pde3a), mRNA.
ACCESSION   NM_018779
VERSION     NM_018779.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Krause,P.N., McGeorge,G., McPeek,J.L., Khalid,S., Nelin,L.D.,
            Liu,Y. and Chen,B.
  TITLE     Pde3a and Pde3b regulation of murine pulmonary artery smooth muscle
            cell growth and metabolism
  JOURNAL   Physiol Rep 12 (20), e70089 (2024)
   PUBMED   39435735
  REMARK    GeneRIF: Pde3a and Pde3b regulation of murine pulmonary artery
            smooth muscle cell growth and metabolism.
REFERENCE   2  (bases 1 to 10794)
  AUTHORS   Van Wauwe,J., Mahy,A., Craps,S., Ekhteraei-Tousi,S., Vrancaert,P.,
            Kemps,H., Dheedene,W., Donate Puertas,R., Trenson,S.,
            Roderick,H.L., Beerens,M. and Luttun,A.
  TITLE     PRDM16 determines specification of ventricular cardiomyocytes by
            suppressing alternative cell fates
  JOURNAL   Life Sci Alliance 7 (12), e202402719 (2024)
   PUBMED   39304345
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10794)
  AUTHORS   Skogestad,J., Albert,I., Hougen,K., Lothe,G.B., Lunde,M.,
            Eken,O.S., Veras,I., Huynh,N.T.T., Borstad,M., Marshall,S.,
            Shen,X., Louch,W.E., Robinson,E.L., Cleveland,J.C. Jr.,
            Ambardekar,A.V., Schwisow,J.A., Jonas,E., Calejo,A.I., Morth,J.P.,
            Tasken,K., Melleby,A.O., Lunde,P.K., Sjaastad,I., Carlson,C.R. and
            Aronsen,J.M.
  TITLE     Disruption of Phosphodiesterase 3A Binding to SERCA2 Increases
            SERCA2 Activity and Reduces Mortality in Mice With Chronic Heart
            Failure
  JOURNAL   Circulation 147 (16), 1221-1236 (2023)
   PUBMED   36876489
  REMARK    GeneRIF: Disruption of Phosphodiesterase 3A Binding to SERCA2
            Increases SERCA2 Activity and Reduces Mortality in Mice With
            Chronic Heart Failure.
REFERENCE   4  (bases 1 to 10794)
  AUTHORS   Bork,N.I., Kuret,A., Cruz Santos,M., Molina,C.E., Reiter,B.,
            Reichenspurner,H., Friebe,A., Skryabin,B.V., Rozhdestvensky,T.S.,
            Kuhn,M., Lukowski,R. and Nikolaev,V.O.
  TITLE     Rise of cGMP by partial phosphodiesterase-3A degradation enhances
            cardioprotection during hypoxia
  JOURNAL   Redox Biol 48, 102179 (2021)
   PUBMED   34763298
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   5  (bases 1 to 10794)
  AUTHORS   Coenen,D.M., Heinzmann,A.C.A., Oggero,S., Albers,H.J., Nagy,M.,
            Hague,P., Kuijpers,M.J.E., Vanderwinden,J.M., van der Meer,A.D.,
            Perretti,M., Koenen,R.R. and Cosemans,J.M.E.M.
  TITLE     Inhibition of Phosphodiesterase 3A by Cilostazol Dampens
            Proinflammatory Platelet Functions
  JOURNAL   Cells 10 (8), 1998 (2021)
   PUBMED   34440764
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10794)
  AUTHORS   Sun,B., Li,H., Shakur,Y., Hensley,J., Hockman,S., Kambayashi,J.,
            Manganiello,V.C. and Liu,Y.
  TITLE     Role of phosphodiesterase type 3A and 3B in regulating platelet and
            cardiac function using subtype-selective knockout mice
  JOURNAL   Cell Signal 19 (8), 1765-1771 (2007)
   PUBMED   17482796
  REMARK    GeneRIF: Role of Pde3a in regulating platelet and cardiac function
            using subtype-selective knockout mice is reported.
REFERENCE   7  (bases 1 to 10794)
  AUTHORS   Omori,K. and Kotera,J.
  TITLE     Overview of PDEs and their regulation
  JOURNAL   Circ Res 100 (3), 309-327 (2007)
   PUBMED   17307970
  REMARK    Review article
REFERENCE   8  (bases 1 to 10794)
  AUTHORS   Han,S.J., Vaccari,S., Nedachi,T., Andersen,C.B., Kovacina,K.S.,
            Roth,R.A. and Conti,M.
  TITLE     Protein kinase B/Akt phosphorylation of PDE3A and its role in
            mammalian oocyte maturation
  JOURNAL   EMBO J 25 (24), 5716-5725 (2006)
   PUBMED   17124499
  REMARK    GeneRIF: These data indicate that activation of PDE3A by
            PKB/Akt-mediated phosphorylation plays a role in the control of
            PDE3A activity in mammalian oocytes.
REFERENCE   9  (bases 1 to 10794)
  AUTHORS   Masciarelli,S., Horner,K., Liu,C., Park,S.H., Hinckley,M.,
            Hockman,S., Nedachi,T., Jin,C., Conti,M. and Manganiello,V.
  TITLE     Cyclic nucleotide phosphodiesterase 3A-deficient mice as a model of
            female infertility
  JOURNAL   J Clin Invest 114 (2), 196-205 (2004)
   PUBMED   15254586
  REMARK    GeneRIF: Results provide evidence indicating that resumption of
            meiosis in vivo and in vitro requires cyclic nucleotide
            phosphodiesterase 3A activity.
REFERENCE   10 (bases 1 to 10794)
  AUTHORS   Shitsukawa,K., Andersen,C.B., Richard,F.J., Horner,A.K.,
            Wiersma,A., van Duin,M. and Conti,M.
  TITLE     Cloning and characterization of the cyclic guanosine
            monophosphate-inhibited phosphodiesterase PDE3A expressed in mouse
            oocyte
  JOURNAL   Biol Reprod 65 (1), 188-196 (2001)
   PUBMED   11420239
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF099187.1 and AC155835.3.
            
            On May 29, 2018 this sequence version replaced NM_018779.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF099187.1, AK052374.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4205              AF099187.1         1-4205
            4206-10794          AC155835.3         30436-37024         c
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 71.87 cM"
     gene            1..10794
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /note="phosphodiesterase 3A, cGMP inhibited"
                     /db_xref="GeneID:54611"
                     /db_xref="MGI:MGI:1860764"
     exon            1..1275
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             322..3747
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /EC_number="3.1.4.17"
                     /note="cGMP-inhibited phosphodiesterase; cGMP-inhibited
                     3',5'-cyclic phosphodiesterase A; CGI-PDE A; cyclic
                     GMP-inhibited phosphodiesterase A"
                     /codon_start=1
                     /product="cGMP-inhibited 3',5'-cyclic phosphodiesterase
                     3A"
                     /protein_id="NP_061249.1"
                     /db_xref="CCDS:CCDS20676.1"
                     /db_xref="GeneID:54611"
                     /db_xref="MGI:MGI:1860764"
                     /translation="MAVRGEAAQDLAKPGLGGASPARVARGNHRHRGESSPSPRGSGC
                     CWRALALQPLRRSPQLSSALCAGSLSVLLALLVRLVGGEVGGELEKSQEAAAEEEEEE
                     GARGGVFPGPRGGAPGGGAQLSPWLQPAALLFSLLCAFFWMGLCLLRAGVRLPLAVAL
                     LAACCAGEALVQLSLGVGDGRLLSLPAAGVLLSCLGGATWLVLRLRLGVLMVAWTSVL
                     RTVALVSLERFKVAWRPYLAYLAAVLGLLLARYAEQILPQCSGPAPPRERFGSQLSAR
                     TKEEIPGWKRRRRSSSVVAGEMSGCSGKSHRRTSLPCIPREQLMGHSEWDHKRGPRGS
                     QSGTSITVDIAVMGEAHGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRV
                     NPTVTFSENYTCSDSEEGLEKDKQAISKRLRRSLPPGLLRRVSSTWTTTTSATGLPTL
                     EPAPVRRDRSASIKPHEAPSPSAVNPDSWNAPGLTTLTKSRSFTSSYAVSAANHVKAK
                     KQNRPGGLAKISPVPSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRGPGSHRALT
                     YTQSAPDLSPQIPPPSVICSSCGRPYSQGNPADGPSERSGPAMLKPNRTDDTSQVTSD
                     YETNNNSDSSDILQNEEEAECQREPQRKASACGTYTSQTMIFLDKPILAPEPLVMDNL
                     DSIMDQLNTWNFPIFDLMENIGRKCGRILSQVSYRLFEDMGLFEAFKIPVREFMNYFH
                     ALEIGYRDIPYHNRIHATDVLHAVWYLTTQPIPGLPSVIGDHGSASDSDSDSGFTHGH
                     MGYVFSKMYHVPDDKYGCLSGNIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQA
                     VLYNDRSVLENHHAAAAWNLFMSRPEYNFLVNLDHVEFKHFRFLVIEAILATDLKKHF
                     DFVAKFNAKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLRWTEGIASEF
                     YEQGDEEASLGLPISPFMDRSAPQLANLQESFISHIVGPLCHSYDSAGLMPGKWVDDS
                     DDSGDTDDPEEEEEEAETPHEDEACESSIAPRKKSFKRRRIYCQITQHLLQNHMMWKK
                     VIEEEQCLSGTENQSLDQVPLQHPSEQIQAIKEEEEEKGKPRAEETLAPQPDL"
     exon            1276..1326
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1581
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1736
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1737..1861
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..2081
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2167
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2168..2322
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2460
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2461..2572
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2573..2686
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2687..2886
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2887..3090
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3091..3246
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3247..3505
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            3506..10794
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7345..7350
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /note="hexamer: AATAAA"
     polyA_site      7368
                     /gene="Pde3a"
                     /gene_synonym="A930022O17Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtgttaaaat cccgaagtag gaagagaccc cgcagggtat aaactctggc tgagggtgga
       61 gcggaggact tttaagagag aaaggaaagg agagagggag agggagaggg agagagagag
      121 agagaaagag aaaaggagag gagtgggggt tgaaaattcc aaggctgatc ctgctagctg
      181 ccaacttgaa gtcttcagct cgcactcttg agttctctgc aaggctgaaa cttcgcgtag
      241 atccggaaga agtgattccg agagtgggct cgagaagagc gcggacgcgc gagtgggacg
      301 ggtgggggtt gtccgcagag catggcagtg cggggcgagg ccgcccaaga cctagccaag
      361 ccgggcctcg gaggggcgag tcccgcccgt gtggcaagag ggaaccaccg ccatcgtggc
      421 gagtcctctc catcccctcg gggctccggc tgttgttgga gagccctggc gctgcagccg
      481 ctccggcgct cgcctcaact ctcctcggcg ctgtgcgcgg gctccctgtc cgtgctgctg
      541 gcgctgctgg tgaggctggt cggcggggag gtcggaggcg agctggagaa gagtcaggag
      601 gcggcggccg aggaggagga ggaggaagga gcccgagggg gcgtcttccc gggccctcgg
      661 ggaggtgctc ccgggggcgg cgcgcagctc agcccttggc tgcagccggc cgcgctgctc
      721 ttcagtctcc tgtgcgcctt cttctggatg ggcttgtgcc tgctgcgcgc cggggtgcgc
      781 ctgcctctgg ccgtcgcgct gttggccgcc tgctgcgccg gggaagcgct ggtgcagctc
      841 tccttgggcg tgggggacgg tcgcctgctg tcgctgcccg ccgcgggggt cctgctcagc
      901 tgcctaggtg gcgcgacatg gctggtgctg aggctgaggc tgggcgtcct catggtcgcc
      961 tggactagcg tgcttaggac ggtggccctg gtttctctgg agaggttcaa ggtcgcctgg
     1021 agaccttacc tggcatactt ggcggccgtg cttgggctgc tgctggccag gtacgccgag
     1081 cagatcctgc cgcagtgctc cgggccggct ccgcccaggg agcggtttgg gtcccagttg
     1141 agcgcgagga ccaaggaaga gattccgggg tggaagagga ggaggcggtc cagctcggtg
     1201 gtggccggcg agatgtccgg ctgcagtggc aagtctcacc ggaggacctc cctgccctgc
     1261 atacccaggg agcagctcat gggccattca gaatgggacc acaagagagg gccacgagga
     1321 tcccagtcag gaaccagcat cactgtggat attgctgtga tgggtgaggc ccatggcctc
     1381 attaccgacc tcctggccga cccttctctg cccccgaatg tgtgtacatc cttgagggcc
     1441 gtaagcaact tgctcagcac ccagctcacc ttccaggcca tccacaagcc cagagtgaac
     1501 cccaccgtga ccttcagtga gaactatacc tgctcggact ctgaggaagg cttggagaaa
     1561 gacaagcagg ccatttccaa gcgcctgaga agaagtttgc ctccaggctt actgagaaga
     1621 gtctcgtcaa cttggacaac taccacctct gccacaggtc tgcccacctt ggagcctgcg
     1681 ccagtgcgga gggaccgcag tgccagcata aagccgcacg aagccccttc acccagtgct
     1741 gtcaatcctg actcttggaa tgccccaggg ttgacgactc tcaccaaaag cagatccttc
     1801 acttcatcct atgctgtctc tgcagctaac catgtaaaag ctaaaaagca aaacaggcca
     1861 ggtggccttg ctaaaatttc ccccgtcccc tcaccctcct cctcgcctcc ccagggatca
     1921 cctgccagca gtcctgtcag caacagtgct tcacaacagt tcccagaatc ccctgaagta
     1981 accatcaagc gagggcctgg atctcacagg gccttaactt acacccaaag tgcccctgac
     2041 ctgtcccctc agatcccacc cccgtctgtc atatgtagca gctgtggcag accttattcg
     2101 caagggaatc ctgctgatgg accctcagag agaagtggcc cagccatgct gaaaccaaac
     2161 agaacagatg acacttctca agttacctcc gattatgaga ccaacaacaa cagtgacagc
     2221 agtgacattc tgcagaatga agaggaagct gagtgccaga gagaacccca gaggaaagca
     2281 tcggcttgtg gcacctatac ttcccagacc atgatcttcc tggacaaacc aattcttgct
     2341 ccagaacccc tggtcatgga taacctggac tcaattatgg atcagttgaa cacctggaat
     2401 tttccaattt ttgatttaat ggaaaatata ggaagaaaat gtggccgtat tctgagccag
     2461 gtatcataca gactgtttga agacatgggg ctctttgaag cctttaaaat cccggttagg
     2521 gagtttatga attactttca tgctttggag atcggataca gggacattcc ttatcataac
     2581 agaatccatg ccaccgatgt tctacacgct gtgtggtatc tcacaacaca gccgattcct
     2641 ggcctcccaa gtgtgattgg tgatcacggc tcagcaagcg actccgattc tgacagtgga
     2701 tttacacacg gacacatggg atatgtgttt tcaaaaatgt atcacgtgcc agatgacaaa
     2761 tatggatgcc tgtctgggaa tattccagcc ctagagttga tggctctgta cgttgctgca
     2821 gccatgcatg actacgatca cccagggagg acaaatgcct tcctggttgc cactagtgcc
     2881 cctcaggcgg tgctatacaa cgaccgttct gttctggaga accatcacgc cgctgcagcc
     2941 tggaatctct tcatgtcccg gccagagtat aacttcttag ttaacctgga ccacgtggaa
     3001 ttcaagcact tccgcttcct agtcattgaa gcgattttgg ctactgacct gaagaaacac
     3061 tttgactttg tagccaaatt taacgccaag gtaaacgatg atgtcgggat agattggacc
     3121 aatgaaaacg accgtctcct ggtgtgtcag atgtgtataa agctggctga catcaacggg
     3181 cctgctaaat gcaaagaact ccacctgcgg tggacagagg gcatcgccag cgagttttat
     3241 gagcagggcg atgaagaagc cagcctcggc ttgcccataa gtccctttat ggaccgctct
     3301 gccccgcagc tggccaatct tcaggaatcc tttatctcac acatcgtggg tcctctctgc
     3361 cactcctatg actctgcggg actcatgcca ggaaaatggg tagatgacag tgatgattcc
     3421 ggagacaccg atgacccgga agaagaggag gaagaggctg aaacaccaca cgaggacgaa
     3481 gcctgtgaaa gcagtatagc tccccgaaag aaaagtttca aaagaaggag aatctactgc
     3541 caaataactc agcaccttct gcagaaccac atgatgtgga agaaagtgat tgaagaggaa
     3601 cagtgtttat caggcacaga gaaccagtcc ctggaccagg tccctctgca gcatccctca
     3661 gagcagatcc aggctatcaa ggaggaagag gaagagaagg ggaagccaag agcagaggag
     3721 accctggctc cacagccaga cctgtgacca tgggtagagc gagctgtgtt tgcagacaga
     3781 atgactcgtc tcagacattt tctaagccag cacacttaga cacaacactg tagattacca
     3841 gaagattggc aaatggctta agtgtttggg ggaattcttc acattttgtg tgtttatttt
     3901 tacagtgagg gacagcgctg aagactctag ctcaaagaag cttttttcac tttgcgacac
     3961 cagcgtctaa agattgtttt ataagaaaga aattatatgt gtgtgtataa attcccacat
     4021 aggctcatgt aagactcgta ttcacgcatg cacacatgca cactgtgagc taggatgcct
     4081 gactccacaa ggtagtaaca gtcatattaa gatatgtaga ggtcactgtg acatagaaag
     4141 tcataaagga caagaactca tgaggaactg tgcagcttag agaggaaaca ggctccagac
     4201 tgagcacctt ttgttctccc tgccgagggg tgagcatttc agaacatggt tggaattcta
     4261 atgcatactg ccaactgtgt gtgttgtgtg tgtgtgagag agagagagag agagacagac
     4321 agacagacag aggggttggt cttgtacatg tgtgcatgag aaaagggaga tgtttatgac
     4381 tttgacttta gttattccta gaatagctgt agcaggtgat gaagtccatg tgaacacaca
     4441 gagggaaatc ctttctgcag tgtctttgaa tgaatggcag cccccctttt agcttctaag
     4501 aaggttcctt agtagaggga gggcttatct gggtcatatg attggtacca ctgctacctg
     4561 ccacttgggg gcactctacc accagcctca cactcagaag actaaggctt tctggtctac
     4621 cctggcttaa tgaatgtata aggggtacat gtcactcact catctaggat caattagcat
     4681 ggaaggggat ggtgttcttt actggtggat acccaagagg agctagaggg ggctctccat
     4741 caggtaagag ttctaacagg gcactggaaa agtcttgtta aagtatctat agcaatgata
     4801 ccctatgtct tcgcaatttg atgcaagtta tgttgtaata aagaagaaag tcaacaaact
     4861 caccgtaggt gctgagacaa agggatgaag gcaaaatgtc aacttagggt ttggaagttg
     4921 gcctggggaa aactactcct tggcaactgt tgaagattgt tagctctgac ctccctgttg
     4981 tggtcttcca tacactgtgt gaaagcatag catggttttg cagcctggtc catggtcatg
     5041 cccctttttg aaaggaagat gggtgggatg ttattcttta aggatagact attggaagct
     5101 atttgtgtta ttttgtttgt tttctacact tgtttatgct gtgagccaaa cctctattta
     5161 aaagttgata ctcactttca atattttatt ggatattatt acatatgtca tgactagtta
     5221 ccatgatgta aatataaaga agtttttgtt gttgttgttg tttctgtaga tagaaaactt
     5281 tttttaaaga aaaaaagggg aaagggaaac attttatagt tatattttaa tcaccgtttt
     5341 tatacattgt agctttctcc aagcacagta gttaggtgcg ggtttattgt ggagtttgag
     5401 agccaccagc catctgccta ctctaatttg attcatgatc tgatacagtt gattgctgtt
     5461 aaattaaggc attgaaaaga tagcatcttt tgctaactca attaatttga agtctttccc
     5521 tgtctgatga aagcctttct taagcaaatt gttagtattt ctcctaaact ctgttgcttg
     5581 tagattctgg tccagtgctg tgttcacaga ctcctagtca tagcctctga gacctgtgca
     5641 taaatttcta ccttttcaca tccttgatta tttacattaa aaatcccttt gtctcccata
     5701 catggattat gtatccttcc cttcaaaaag caacattttc ttgtatttca cagcattttt
     5761 tcccctaata gacaaaaatg aaatgaaaaa tcaagtattt cagggtagat tttttttttt
     5821 ctagttcaga ggacaatgca aggaggattg gaaggattcg ggcctcaccc agacaattag
     5881 aatgtgtttc tatgcatttc tgagatagtg ccagggcttc ggcagttagc agattctgaa
     5941 tattttttct ttttttatgg tggccatatg tctctgttgg taaatctact ccgaagcaag
     6001 aatccttctt cagtgaaggc aaataaatgt aactcttgcc ctgcaatagg aatgatagac
     6061 agagcactta cattcatcta cccttcactc agtagctcat ccacgtgttc aagtggacac
     6121 atgtctgcaa cataaatatg agtcgtcaaa caagctaggc atggagtgag gggattggcc
     6181 tatccattgt gagtgtttat attggaagtc tgaacaaagc tgcagagtct gagtaggagt
     6241 caggctggtt acatcatgag gatctcagca aactgcctag attttcttgt tttaaagtac
     6301 acgaagtaac aggataatga gcagagcatt agaatctatc tgttctagaa ttgtgctcat
     6361 tcatccaggc agattgattt ttttttcctc aaaaaacaag ttgttgttgt tctcaaccca
     6421 aaaggcaaca aactgaatgg aagggtggaa acccaccaag tgtgtgacct aaagagccac
     6481 ctaataattc tcgaagcgta tcaaatgaca tcaaaagtat tttccatgaa tgatgttgtc
     6541 caccttcttg gtgacagcaa tattactgta ttcacctcca acttcagagt taacttcctg
     6601 tggtgccaat gtgtttgatg tgacttacta cagttttata tgagcctata caggtgccat
     6661 ttgataaact caggtctccc agtggagggg tctctaccca cttagggaaa agatagtttt
     6721 atagatagcc ataatgtgtc agtggtagga gaaattggga accagctgct ctctgtggag
     6781 taaaagccta agtgtgttct gcatgttatc catggtattt tcaacataat aatggaatgt
     6841 ctttatggtg accgaagcca gctgaccttt tgagtagcat ttccttagta gagagaaccc
     6901 tttttcctgt tttacttaac tgtaagaaaa aaaaaaagta ctcgtttatg taagacctgg
     6961 ttttgttctc agtaggacat gcactcagta gttagtatat tgtcatttcc tggcattaag
     7021 agaattgcct cctgtttgaa aaagcatctg ccttcctcta tcgttgcacc ctgttctgtt
     7081 tgggttttac tccagaacaa gcattacaca ttgcagtccc aggaaagctg aggagaagga
     7141 agtgaatgaa tgccctgcag actctttggt agctgtagat atttgcaatt tccctgtgtc
     7201 ctctggcctt tctttcccag agaactttct tgaaggtaaa agctgtgcaa aaggcatgag
     7261 actcaggcct actctttgtt tgttaaatga tggaaaagta taaattattt tctaagtaat
     7321 aaaaatatga aaattatcat tgtaaataaa gtctaaagct tgaaatgact tctttataga
     7381 agtggagtgt ttgtgtggtg aacaactcct ggccaatagg aaaagaagaa tctggaattt
     7441 ctgtctgttc tgcttgagga cacactcttg gtgcctcctt gaggcgggca cctccattga
     7501 tcttccttag ggtcttggaa gcataaagag aggcttctta gccttctctc taatcctgaa
     7561 tctcattgga tgcctccctt ggtttttgtg tcattagcaa tttaattgcc tttacaattg
     7621 tctgaaggct actgcaacag agttctatgg ccttccacag actaaatcaa tattctactc
     7681 tttaggtgtg tcccagataa ctaaatgaca caataaccat gtgctttgaa actgaagtca
     7741 ttttcttgca tcatcttgcc ccctgaacaa tgaaatatta cacgatcctt gttctaggtc
     7801 actcatttct tcatgaattt gtaaagggaa attatgaatt ttctgatagc aacatgttta
     7861 aaaatctatc caaacatatt aaaactgaag atcgataaaa cagttcacca tttcaagtag
     7921 aagagacttc agaattaaat tcatcttaga atgtctaatt aagctgaaat ccatcaattt
     7981 ggggtcctgg accccaaatt tatgtcatgt taattcatcc tccagtaaat gccccagtat
     8041 tcattgaaaa ataaattaga agtggattaa gttgttagcc ctgactaaac taaaccatac
     8101 acttcagtct agatggttta ttgtgctttt ggtgagtatt tttatgcctc caatgaggga
     8161 accataatta gagaataaaa acatactagt agtatacatt atacaaggtg gatatcaata
     8221 aactatgttg ctaccttatg ctttgcatgg gctaaaatgt tcttatttcc tatactacct
     8281 aatactgctt tttgccttac ctagtaattg gctttatgat catctagtaa attaacccta
     8341 acaactgatt tgatgttgtg actaagatat ttgaccttct ctgtgtggat actttaaata
     8401 tgtctgcaga tgcaaaaatg tagttggtgg gacctaatct gctacttcaa agcataatgt
     8461 tgatccttga gtttttaacc taggaaagac cataagtaca tagactgtgt gtttttgtgc
     8521 tgggctgaag actctaaact tatctcacat ttcttagcat catgttctgg catcttaggt
     8581 gtatgcctcc ccctcaaact ggtgtggaac acagagggaa aagcaccctt cctctaatag
     8641 ataaatgttc actggggccg tgtgtaaccc agactcgttg gtttgcatta agaagcggct
     8701 ctttaatcat caaagctaac aagaagtaat gttaagtctt gtgtgttgtt actgacagaa
     8761 gtatttcaat ttgatttaaa ttcttgaatc acccaccatt ctcttgcatt ttgggggata
     8821 gggattaaaa ttaaccccaa aaagcactgc ttcttggtat cacaattacc atagtctggt
     8881 aggatttaat gtttttctag tgctcttgag gggaaaatga tttttttttt tttggttttt
     8941 cgagacaggg tctctctgta tagtcctggc tgtcctggaa ctcattttgt agaccaagtt
     9001 ggcctcgaac tcagaaatcc acctgcctct gcctcccgag tgctgggatt aaaggtgtgc
     9061 accaccaccc cccggtggaa aatgatttat aagggaatca gtaagtgtac atttaataaa
     9121 gtacatgaag aatttaagtg tcctcagggc agctcacgta gcctgtaaca ccagttccag
     9181 gggatctgac accatcttct gacctccttg gacactgcat atgtgtggta cacagacatc
     9241 tcttcaggca aaacacccat ctgcataaaa taaatttgta caaaaaaata ccaaagaatg
     9301 caagtgccca aattgcaatc taagttcctc cgagttaccc cagtgcacag ggaggcacca
     9361 tccagagatt tatcacaata cattcaaaga tgatacattg tctatagctc agttcctgtg
     9421 agttacccta atcaccagca ggagttcttc agttgcttat catacatttt aagtgatgca
     9481 ttgcctacag cttagttcct ttgagctacc ccaacgcaca gggtgatatc agctagtaga
     9541 ttaccatcag acatttaaaa atgatatttg atctattacc atagattttt attttctgaa
     9601 ttctaataga gttaaaaact caaattcttc cacactaaga aaatgaaaca tcattaattt
     9661 tggctttcac aaataaagga ttgaccttgg gtaaaccacc ccactgtgtt catctgtcat
     9721 aacagtaggg tagactatga aattcctata tagaaaagca acaaaaaaac tgtgtaaagc
     9781 cttgatactt taaatttcca gttatatcgg acatggttct ttcaactgaa agaaactatt
     9841 gcaggaaatt ctcaattctt atgaatagaa aaacaaaaac aaacaaacaa acaaaaaaaa
     9901 cagagaaaaa caacaaaaga aactttggct taaaaatgga gtatttctca ccaattttct
     9961 ttcagtgatc caatattagc aaagacagtg taatcagaaa tgctcttctg ttggaagaga
    10021 gggaaggaag tttttaatgc cagggtccaa atctccccaa ctctatttcc acagcgtggt
    10081 acaaagagat aaaagcaaaa ctgaagatga cataaaactc tgatgcacac ctgtcgagcc
    10141 ttctcacaaa ccagctcagt tgtttgctct ggagtgaaat ggccttgtag gtccatgata
    10201 ctgcctggac ttaattgaac ataaacagca catcttctca ctgctgaatg tgaatcacta
    10261 gtgctctgag ctggtgctga tgagaaggaa ttttctatgg taattacatt tcctttatat
    10321 agcaaaatat tcagacacaa atgtgaaatc aataataact gttttaaatt gtcttctctg
    10381 tatataactt gttggtgaaa taggggtccg gtgacttctc ttttaatatt tttcaattgg
    10441 ttattttgct aactcagaaa accgctatta tatcagatat ttcattttaa ttatttatat
    10501 acatactcaa gagctatact gttatttcca ttaagaaata gtcctaacat atctatatat
    10561 tatatgctaa agggggttca aatgactttt aatatatatt caccaataac taattttaaa
    10621 tcctctgtaa aatatttgca tgagattttt agcatactat atttgcttgt ttttcgcatc
    10681 tatgagtgtc agtattctgc atatattctc acagtagaac gtatacccta atttttttgt
    10741 accagtaaag ctgatctgtc agctgacatg cactggaaat aaatatgtct gaat
//
