LOCUS       NM_001243758            4066 bp    mRNA    linear   ROD 07-MAY-2024
DEFINITION  Mus musculus phosphodiesterase 2A, cGMP-stimulated (Pde2a),
            transcript variant 5, mRNA.
ACCESSION   NM_001243758
VERSION     NM_001243758.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Zhang,X., He,H., Yu,H., Teng,X., Wang,Z., Li,C., Li,J., Yang,H.,
            Shen,J., Wu,T., Zhang,F., Zhang,Y. and Wu,Q.
  TITLE     Maternal RNA transcription in Dlk1-Dio3 domain is critical for
            proper development of the mouse placental vasculature
  JOURNAL   Commun Biol 7 (1), 363 (2024)
   PUBMED   38521877
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4066)
  AUTHORS   Cardarelli,S., Biglietto,M., Orsini,T., Fustaino,V., Monaco,L., de
            Oliveira do Rego,A.G., Liccardo,F., Masciarelli,S., Fazi,F.,
            Naro,F., De Angelis,L. and Pellegrini,M.
  TITLE     Modulation of cAMP/cGMP signaling as prevention of congenital heart
            defects in Pde2A deficient embryos: a matter of oxidative stress
  JOURNAL   Cell Death Dis 15 (2), 169 (2024)
   PUBMED   38395995
  REMARK    GeneRIF: Modulation of cAMP/cGMP signaling as prevention of
            congenital heart defects in Pde2A deficient embryos: a matter of
            oxidative stress.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4066)
  AUTHORS   Carlantoni,C., Liekfeld,L.M.H., Hemkemeyer,S.A., Schreier,D.,
            Saygi,C., Kurelic,R., Cardarelli,S., Kalucka,J., Schulte,C.,
            Beerens,M., Mailer,R.K., Schaffer,T.E., Naro,F., Pellegrini,M.,
            Nikolaev,V.O., Renne,T. and Frye,M.
  TITLE     The phosphodiesterase 2A controls lymphatic junctional maturation
            via cGMP-dependent notch signaling
  JOURNAL   Dev Cell 59 (3), 308-325 (2024)
   PUBMED   38159569
  REMARK    GeneRIF: The phosphodiesterase 2A controls lymphatic junctional
            maturation via cGMP-dependent notch signaling.
REFERENCE   5  (bases 1 to 4066)
  AUTHORS   Delhaye,S., Jarjat,M., Boulksibat,A., Sanchez,C., Tempio,A.,
            Turtoi,A., Giorgi,M., Lacas-Gervais,S., Baj,G., Rovere,C.,
            Trezza,V., Pellegrini,M., Maurin,T., Lalli,E. and Bardoni,B.
  TITLE     Defects in AMPAR trafficking and microglia activation underlie
            socio-cognitive deficits associated to decreased expression of
            phosphodiesterase 2 a
  JOURNAL   Neurobiol Dis 191, 106393 (2024)
   PUBMED   38154608
  REMARK    GeneRIF: Defects in AMPAR trafficking and microglia activation
            underlie socio-cognitive deficits associated to decreased
            expression of phosphodiesterase 2 a.
REFERENCE   6  (bases 1 to 4066)
  AUTHORS   Mongillo,M., Tocchetti,C.G., Terrin,A., Lissandron,V., Cheung,Y.F.,
            Dostmann,W.R., Pozzan,T., Kass,D.A., Paolocci,N., Houslay,M.D. and
            Zaccolo,M.
  TITLE     Compartmentalized phosphodiesterase-2 activity blunts
            beta-adrenergic cardiac inotropy via an NO/cGMP-dependent pathway
  JOURNAL   Circ Res 98 (2), 226-234 (2006)
   PUBMED   16357307
REFERENCE   7  (bases 1 to 4066)
  AUTHORS   Isenberg,J.S., Ridnour,L.A., Perruccio,E.M., Espey,M.G., Wink,D.A.
            and Roberts,D.D.
  TITLE     Thrombospondin-1 inhibits endothelial cell responses to nitric
            oxide in a cGMP-dependent manner
  JOURNAL   Proc Natl Acad Sci U S A 102 (37), 13141-13146 (2005)
   PUBMED   16150726
REFERENCE   8  (bases 1 to 4066)
  AUTHORS   Wu,A.Y., Tang,X.B., Martinez,S.E., Ikeda,K. and Beavo,J.A.
  TITLE     Molecular determinants for cyclic nucleotide binding to the
            regulatory domains of phosphodiesterase 2A
  JOURNAL   J Biol Chem 279 (36), 37928-37938 (2004)
   PUBMED   15210692
  REMARK    GeneRIF: cGMP binding to PDE2 GAF-B activates the enzyme rapidly,
            stoichiometrically, and in an all or none fashion, rather than
            variably over a large range of cyclic nucleotide concentrations
REFERENCE   9  (bases 1 to 4066)
  AUTHORS   Martinez,S.E., Wu,A.Y., Glavas,N.A., Tang,X.B., Turley,S., Hol,W.G.
            and Beavo,J.A.
  TITLE     The two GAF domains in phosphodiesterase 2A have distinct roles in
            dimerization and in cGMP binding
  JOURNAL   Proc Natl Acad Sci U S A 99 (20), 13260-13265 (2002)
   PUBMED   12271124
REFERENCE   10 (bases 1 to 4066)
  AUTHORS   Cherry,J.A. and Davis,R.L.
  TITLE     A mouse homolog of dunce, a gene important for learning and memory
            in Drosophila, is preferentially expressed in olfactory receptor
            neurons
  JOURNAL   J Neurobiol 28 (1), 102-113 (1995)
   PUBMED   8586960
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129079.5 and AC129609.3.
            
            On Nov 18, 2022 this sequence version replaced NM_001243758.1.
            
            Transcript Variant: This variant (5) differs in the 5' UTR, uses an
            alternate splice site in the coding region and initiates
            translation at an alternate start codon, compared to variant 1. The
            encoded isoform (5) is longer and has a distinct N-terminus,
            compared to isoform 6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK158560.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849379, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-173               AC129079.5         23258-23430         c
            174-240             AC129079.5         22518-22584         c
            241-330             AC129609.3         33763-33852
            331-419             AC129609.3         36934-37022
            420-529             AC129609.3         46963-47072
            530-585             AC129609.3         48029-48084
            586-645             AC129609.3         52780-52839
            646-787             AC129609.3         53369-53510
            788-840             AC129609.3         54002-54054
            841-915             AC129609.3         54246-54320
            916-957             AC129609.3         54406-54447
            958-1026            AC129609.3         54926-54994
            1027-1157           AC129609.3         55204-55334
            1158-1269           AC129609.3         55691-55802
            1270-1309           AC129609.3         56216-56255
            1310-1373           AC129609.3         56364-56427
            1374-1446           AC129609.3         56770-56842
            1447-1624           AC129609.3         56963-57140
            1625-1737           AC129609.3         57349-57461
            1738-1815           AC129609.3         58311-58388
            1816-1938           AC129609.3         59488-59610
            1939-2009           AC129609.3         60069-60139
            2010-2132           AC129609.3         60399-60521
            2133-2220           AC129609.3         60834-60921
            2221-2268           AC129609.3         61084-61131
            2269-2343           AC129609.3         62109-62183
            2344-2443           AC129609.3         62327-62426
            2444-2556           AC129609.3         62698-62810
            2557-2595           AC129609.3         63321-63359
            2596-2702           AC129609.3         63506-63612
            2703-4066           AC129609.3         63848-65211
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 54.57 cM"
     gene            1..4066
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /note="phosphodiesterase 2A, cGMP-stimulated"
                     /db_xref="GeneID:207728"
                     /db_xref="MGI:MGI:2446107"
     exon            1..173
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..2910
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /EC_number="3.1.4.17"
                     /note="isoform 5 is encoded by transcript variant 5;
                     cGMP-dependent 3',5'-cyclic phosphodiesterase; cyclic
                     GMP-stimulated phosphodiesterase"
                     /codon_start=1
                     /product="cGMP-dependent 3',5'-cyclic phosphodiesterase
                     isoform 5"
                     /protein_id="NP_001230687.1"
                     /db_xref="CCDS:CCDS85351.1"
                     /db_xref="GeneID:207728"
                     /db_xref="MGI:MGI:2446107"
                     /translation="MALGLAPFSASWDADEGLSGSLRNPVLSPHIWERRGQQVFLKPD
                     EPPPQPCADSLQDALLSLGAVIDIAGLRQAARDALSAVLPKVETVYTYLLDGESRLVC
                     EDPPHELPQEGKIREAVISQKRLSCNGLGPSDLLGKPLARLVAPLAPDMQVLVIPLLD
                     KETGSVAAVILVHCGQLSDSEEQSLQVVEKHALVALRRVQALQQRRPEAVQNTSVDAS
                     EDQKDEKGYTDHDRKILQLCGELFDLDATSLQLKVLQYLQQETQATHCCLLLVSEDNL
                     QLSCKVIGDKVLGEEVSFPLTMGRLGQVVEDKQCIQLKDLTSDDVQQLQNMLGCELQA
                     MLCVPVISRATDQVVALACAFNKLGGDFFTDEDEHVIQHCFHYTGTVLTSTLAFQKEQ
                     KLKCECQALLQVAKNLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVF
                     DGGVVDDESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRNIL
                     CFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHSLLYKKVNEAQ
                     YRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFANFTYTPRSLPEDDTSMAI
                     LSMLQDMNFINNYKIDCPTLARFCLMVKKGYRDPPYHNWMHAFSVSHFCYLLYKNLEL
                     SNYLEDIEIFALFISCMCHDLDHRGTNNSFQVASKSVLAALYSSEGSVMERHHFAQAI
                     AILNTHGCNIFDHFSRKDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNR
                     QHHRLLLCLLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDRE
                     KAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHKFTIRGLPS
                     NNSLDFLDEEYEVPDLDGTRAPVNGCCSLEG"
     exon            174..240
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            241..330
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            331..419
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            420..529
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            530..585
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            586..645
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            646..787
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            788..840
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            841..915
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            916..957
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1026
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1157
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1269
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1309
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1373
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1446
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1447..1624
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1625..1737
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1815
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..1938
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            1939..2009
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2132
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2220
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2268
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2343
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2443
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2556
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2557..2595
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2596..2702
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     exon            2703..4066
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4043..4048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /note="hexamer: AATAAA"
     polyA_site      4066
                     /gene="Pde2a"
                     /gene_synonym="CGS-PDE; cGSPDE"
                     /note="major polyA site"
ORIGIN      
        1 tgagcacaga ggcggctctg cagagaaatg cgctcccagc tctcggggtc ccaccgcgga
       61 actgcggact agatggccct ggggcttgca cccttttcag cctcctggga cgccgatgaa
      121 ggcctatctg gtagccttag gaacccagtg ctctcacccc acatttggga acggcggggc
      181 cagcaggtct tcctcaagcc ggacgagccg ccgccgcagc catgcgccga cagcctgcag
      241 gatgctttgc tgagcctagg cgccgttatc gacattgctg gcctgcgaca ggctgccagg
      301 gatgccctct cagccgtgct ccccaaagtg gagactgtct acacctacct gctagatggg
      361 gagtccagac tggtgtgtga ggacccccct catgagctgc cacaggaagg aaaaattcga
      421 gaagctgtga tctctcagaa gcggctgagt tgcaatgggc tgggaccttc ggacctactg
      481 ggaaagccct tggccaggct ggtggctcca ctggctcctg acatgcaagt gctggtcata
      541 cccctgctgg acaaggagac tggaagtgtg gcagctgtca tcttggtgca ctgtggccag
      601 ctgagtgaca gtgaggaaca gagcttgcag gtggtagaga agcacgctct ggtagccctg
      661 cggagggtgc aggccctgca gcagcgcagg cctgaagctg ttcagaacac gtcagtggat
      721 gcctctgaag atcaaaagga tgagaagggg tacaccgacc atgaccgaaa gatcctgcaa
      781 ctgtgtgggg aactctttga cttggatgct acttctctgc agctcaaagt ccttcaatat
      841 ctgcagcagg agacacaggc cactcactgc tgcctcctgc tggtgtcgga ggacaatcta
      901 cagctttcct gcaaggtcat tggagacaaa gtgctgggag aagaggtcag ctttccattg
      961 accatgggac gtctggggca ggtggtggaa gacaagcagt gtatccagtt gaaggaccta
     1021 acctctgacg atgtgcaaca gctgcaaaac atgttgggtt gtgagctgca ggctatgctg
     1081 tgtgtccctg tcatcagccg agccactgac caggtggtgg ccctggcttg cgccttcaac
     1141 aagcttggag gagatttctt cacagatgag gatgaacacg tgatccaaca ctgcttccac
     1201 tacacaggca cggtgctcac cagcaccttg gccttccaga aggagcagaa gctcaagtgt
     1261 gagtgccagg ctcttctcca agtggcaaag aacctcttca cccacctgga tgatgtctct
     1321 gtcctgctac aggagatcat cacggaagcc agaaacctca gcaacgcaga gatctgctcg
     1381 gtgttcctgc tggatcagaa cgagctggtg gccaaggtgt tcgatggtgg cgttgtggac
     1441 gatgagagtt atgagatccg catcccggcg gaccaaggca tcgcgggcca cgtggcgact
     1501 acgggccaga tcctgaacat cccagatgca tatgcccatc cgcttttcta tcgcggcgta
     1561 gatgacagca ctggcttccg cacgcgcaac attctctgct tccctatcaa gaacgagaac
     1621 caggaggtca ttggtgtggc tgagctagtg aacaagatca atgggccatg gttcagcaag
     1681 tttgatgagg acctggccac agccttctcc atctactgtg gcatcagcat cgcccactct
     1741 ctcctataca aaaaggtgaa tgaagcccaa taccgcagcc acctggccaa tgagatgatg
     1801 atgtatcata tgaaggtctc tgatgatgaa tacaccaagc ttctccacga tggcatccaa
     1861 cctgtggccg ccattgactc caactttgcc aactttacct acacgcctcg gtctctgcct
     1921 gaggacgaca cttctatggc catcctgagc atgctgcaag acatgaactt catcaataac
     1981 tacaaaattg actgcccaac tctggcccga ttctgcctga tggtgaagaa aggctaccgg
     2041 gatccaccct accacaactg gatgcacgcc ttctctgtct ctcatttttg ctacctgctc
     2101 tacaagaatc tggagctctc caactacctc gaggacatcg agatctttgc attgtttatt
     2161 tcctgcatgt gtcatgacct ggaccacaga ggcacaaaca actccttcca ggtggcctcg
     2221 aaatctgtgc tggccgcact ctacagctca gagggctctg tcatggagag gcaccacttt
     2281 gctcaagcca ttgctatcct caacacccac ggctgcaata tctttgacca cttctctcgg
     2341 aaggactatc agcgcatgct ggacctgatg agggacatca tcttggctac agacctggca
     2401 caccacctcc gcatcttcaa ggacctgcag aagatggctg aagtgggtta tgaccgaaac
     2461 aacaggcaac accacaggct tcttctgtgc ctcctcatga cctcctgtga cctctctgac
     2521 cagacaaagg gctggaagac caccagaaag attgcagagc tgatctacaa agagttcttc
     2581 tcccagggag acttggagaa ggccatgggc aaccgaccga tggagatgat ggaccgtgag
     2641 aaggcctaca tccctgagct tcagatcagc tttatggagc acattgccat gcctatctac
     2701 aagcttttac aagacctgtt ccccaaggcg gcagagctgt atgaacgtgt ggcctccaac
     2761 cgtgagcact ggaccaaggt gtcccacaag ttcaccatcc gaggcctccc cagtaacaac
     2821 tcgctggatt tcctggacga ggaatacgag gtccccgatt tggacggcac cagagctcct
     2881 gtcaatggct gctgcagcct cgagggctga gggatcccct ctagggaccc tccctgcctg
     2941 gccctccttt cacaactctc taccagtctg tttgatgcat tgggaaccag agccatgggt
     3001 cctgggttct aggccaggat gtcccctgtg acccggcaca tgccaccctt cctgggcttc
     3061 ttcatctttc tcatctgcag aaggcaagca agattaccag tcccacacag actttgccat
     3121 ttgtacacaa gagcacagta gtgaccaggg gcagtggtat gggtctcttc tgcacctctg
     3181 actatccatg cccagtcctg cccaagccgc tccatctctt aagcagcgtc acagaacgtc
     3241 ctgtgacccc gttagagccc tactaggtca gtacccttgt tttcctggag gaatcccagg
     3301 tctctggaga ctccacgatc ctcacctcat ggaaggagga gagactgctg atcgagcaga
     3361 ggatataaaa caagtcacca gctggaagta ccctgagggt gcctctgtgc ttttggtagg
     3421 attgggaccc ttggaggaag ctaactgcca ggttgctcag tgctctgcag gaggaagtgc
     3481 tccagcagag aggctgggaa taatgggcag gaagaattgt caagccagga gacagtcctg
     3541 tacacaccca cacccttcag gcagtgtgac ctgaagcaag tccctttctc tagctaggca
     3601 agacagaatc attagacagc ctagtctctc ttcagatcaa agtctccata gttgtgtgga
     3661 gtccgtgagt ggccgggcat gctctgctgc cctaaggcac agagagccta acttttcttt
     3721 cctctgagtg tatccccttg gtctgagccc ctctcccatt cctgccacca gagaggggcc
     3781 tggcctctcc ccacagggct tctctcctct tcaaggccat atccatatgt gccccccggg
     3841 gcctcaggag actctcccag ggctgggcct cttgggtgag tggccactag cttctcttct
     3901 gttttgttcc ctatgtgtcg tgggtggggg agggggccac ctgccttacc tactctgagt
     3961 tgcctttaga gagatgcatt ttttctagga ctctgtgcac ctgttgtgtg tggtcccgtg
     4021 ggctgagtgc tttgtacatg agaataaatc tatttctttc taccaa
//
