LOCUS       NM_001081033            8457 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus phosphodiesterase 11A (Pde11a), mRNA.
ACCESSION   NM_001081033 XM_001003960
VERSION     NM_001081033.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Sbornova,I., van der Sande,E., Milosavljevic,S., Amurrio,E.,
            Burbano,S.D., Das,P.K., Do,H.H., Fisher,J.L., Kargbo,P., Patel,J.,
            Porcher,L., De Zeeuw,C.I., Meester-Smoor,M.A., Winkelman,B.H.J.,
            Klaver,C.C.W., Pocivavsek,A. and Kelly,M.P.
  TITLE     The Sleep Quality- and Myopia-Linked PDE11A-Y727C Variant Impacts
            Neural Physiology by Reducing Catalytic Activity and Altering
            Subcellular Compartmentalization of the Enzyme
  JOURNAL   Cells 12 (24), 2839 (2023)
   PUBMED   38132157
  REMARK    GeneRIF: The Sleep Quality- and Myopia-Linked PDE11A-Y727C Variant
            Impacts Neural Physiology by Reducing Catalytic Activity and
            Altering Subcellular Compartmentalization of the Enzyme.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8457)
  AUTHORS   Pilarzyk,K., Capell,W.R., Porcher,L., Rips-Goodwin,A. and
            Kelly,M.P.
  TITLE     Biologic that disrupts PDE11A4 homodimerization in hippocampus CA1
            reverses age-related cognitive decline of social memories in mice
  JOURNAL   Neurobiol Aging 131, 39-51 (2023)
   PUBMED   37572526
REFERENCE   4  (bases 1 to 8457)
  AUTHORS   Pilarzyk,K., Porcher,L., Capell,W.R., Burbano,S.D., Davis,J.,
            Fisher,J.L., Gorny,N., Petrolle,S. and Kelly,M.P.
  TITLE     Conserved age-related increases in hippocampal PDE11A4 cause
            unexpected proteinopathies and cognitive decline of social
            associative memories
  JOURNAL   Aging Cell 21 (10), e13687 (2022)
   PUBMED   36073342
  REMARK    GeneRIF: Conserved age-related increases in hippocampal PDE11A4
            cause unexpected proteinopathies and cognitive decline of social
            associative memories.
REFERENCE   5  (bases 1 to 8457)
  AUTHORS   Smith,A.J., Farmer,R., Pilarzyk,K., Porcher,L. and Kelly,M.P.
  TITLE     A genetic basis for friendship? Homophily for membrane-associated
            PDE11A-cAMP-CREB signaling in CA1 of hippocampus dictates mutual
            social preference in male and female mice
  JOURNAL   Mol Psychiatry 26 (12), 7107-7117 (2021)
   PUBMED   34321593
REFERENCE   6  (bases 1 to 8457)
  AUTHORS   Hegde,S., Capell,W.R., Ibrahim,B.A., Klett,J., Patel,N.S.,
            Sougiannis,A.T. and Kelly,M.P.
  TITLE     Phosphodiesterase 11A (PDE11A), Enriched in Ventral Hippocampus
            Neurons, is Required for Consolidation of Social but not Nonsocial
            Memories in Mice
  JOURNAL   Neuropsychopharmacology 41 (12), 2920-2931 (2016)
   PUBMED   27339393
  REMARK    GeneRIF: The data of this study suggest PDE11A is selectively
            required for the proper consolidation of recognition and
            associative social memories.
REFERENCE   7  (bases 1 to 8457)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   8  (bases 1 to 8457)
  AUTHORS   Kelly,M.P., Logue,S.F., Brennan,J., Day,J.P., Lakkaraju,S.,
            Jiang,L., Zhong,X., Tam,M., Sukoff Rizzo,S.J., Platt,B.J.,
            Dwyer,J.M., Neal,S., Pulito,V.L., Agostino,M.J., Grauer,S.M.,
            Navarra,R.L., Kelley,C., Comery,T.A., Murrills,R.J., Houslay,M.D.
            and Brandon,N.J.
  TITLE     Phosphodiesterase 11A in brain is enriched in ventral hippocampus
            and deletion causes psychiatric disease-related phenotypes
  JOURNAL   Proc Natl Acad Sci U S A 107 (18), 8457-8462 (2010)
   PUBMED   20404172
  REMARK    GeneRIF: PDE11A knockout mice show subtle
            psychiatric-disease-related deficits, including hyperactivity in an
            open field, increased sensitivity to the glutamate
            N-methyl-D-aspartate receptor antagonist MK-801, as well as
            deficits in social behaviors
REFERENCE   9  (bases 1 to 8457)
  AUTHORS   Omori,K. and Kotera,J.
  TITLE     Overview of PDEs and their regulation
  JOURNAL   Circ Res 100 (3), 309-327 (2007)
   PUBMED   17307970
  REMARK    Review article
REFERENCE   10 (bases 1 to 8457)
  AUTHORS   Wayman,C., Phillips,S., Lunny,C., Webb,T., Fawcett,L., Baxendale,R.
            and Burgess,G.
  TITLE     Phosphodiesterase 11 (PDE11) regulation of spermatozoa physiology
  JOURNAL   Int J Impot Res 17 (3), 216-223 (2005)
   PUBMED   15800654
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845373.12, AL929133.4 and AL772341.12.
            
            On Dec 5, 2019 this sequence version replaced NM_001081033.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC158125.1, SRR9219382.21507.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1243              AL845373.12        119054-120296       c
            1244-1402           AL845373.12        72523-72681         c
            1403-1492           AL929133.4         53672-53761         c
            1493-1633           AL929133.4         49377-49517         c
            1634-1698           AL929133.4         33243-33307         c
            1699-1831           AL772341.12        216056-216188       c
            1832-1907           AL772341.12        197595-197670       c
            1908-1975           AL772341.12        195099-195166       c
            1976-2068           AL772341.12        194127-194219       c
            2069-2119           AL772341.12        171264-171314       c
            2120-2266           AL772341.12        133697-133843       c
            2267-2374           AL772341.12        133113-133220       c
            2375-2484           AL772341.12        116736-116845       c
            2485-2575           AL772341.12        107900-107990       c
            2576-2676           AL772341.12        104539-104639       c
            2677-2754           AL772341.12        88684-88761         c
            2755-2818           AL772341.12        87037-87100         c
            2819-2893           AL772341.12        80471-80545         c
            2894-2977           AL772341.12        75596-75679         c
            2978-8457           AL772341.12        43568-49047         c
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 44.8 cM"
     gene            1..8457
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /note="phosphodiesterase 11A"
                     /db_xref="GeneID:241489"
                     /db_xref="MGI:MGI:3036251"
     exon            1..1243
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     CDS             332..3133
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /note="cAMP and cGMP phosphodiesterase 11A"
                     /codon_start=1
                     /product="dual 3',5'-cyclic-AMP and -GMP phosphodiesterase
                     11A"
                     /protein_id="NP_001074502.1"
                     /db_xref="CCDS:CCDS38152.1"
                     /db_xref="GeneID:241489"
                     /db_xref="MGI:MGI:3036251"
                     /translation="MAASRLDFGEVETFLDRHPELFEDYLMRKGKQELVDKWLQRHTS
                     GQGASSLRPALAGASSLAQSNAKGSPGIGGGAGPQGSAHSHPTPGGGESAGVPLSPSW
                     ASGSRGDGSLQRRASQKELRKSFARSKAIHVNRTYDEQVTSRAQEPLSSVRRRALLRK
                     ASSLPPTTAHILSALLESRVNLPQYPPTAIDYKCHLKKHNERQFFLELVKDISNDLDL
                     TSLSYKILIFVCLMVDADRCSLFLVEGAAAGKKTLVSKFFDVHAGTPLLPCSSTENSN
                     EVQVPWGKGIIGYVGEHGETVNIPDAYQDRRFNDEIDKLTGYKTKSLLCMPIRNSDGE
                     IIGVAQAINKVPEGAPFTEDDEKVMQMYLPFCGIAISNAQLFAASRKEYERSRALLEV
                     VNDLFEEQTDLEKIVKKIMHRAQTLLKCERCSVLLLEDIESPVVKFTKSFELMSPKCS
                     ADAENSFKESVEKSSYSDWLINNSIAELVASTGLPVNVSDAYQDPRFDAEADQISGFH
                     IRSVLCVPIWNSNHQIIGVAQVLNRLDGKPFDDADQRLFEAFVIFCGLGINNTIMYDQ
                     VKKSWAKQSVALDVLSYHATCSKAEVDKFKAANIPLVSELAIDDIHFDDFSLDVDAMI
                     TAALRMFMELGMVQKFKIDYETLCRWLLTVRKNYRMVLYHNWRHAFNVCQLMFAMLTT
                     AGFQEILTEVEILAVIVGCLCHDLDHRGTNNAFQAKSDSALAQLYGTSATLEHHHFNH
                     AVMILQSEGHNIFANLSSKEYSDLMQLLKQSILATDLTLYFERRTEFFELVRKGDYDW
                     SITSHRDVFRSMLMTACDLGAVTKPWEISRQVAELVTSEFFEQGDRERSELKLTPSAI
                     FDRNRKDELPRLQLEWIDSICMPLYQALVKVNAKLKPMLDSVAANRRKWEELHQKRLQ
                     VSAASPDPASPMVAGEDRL"
     exon            1244..1402
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1492
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1633
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1698
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1831
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1907
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1908..1975
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2068
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2119
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2266
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..2374
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2484
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2575
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2576..2676
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2677..2754
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2755..2818
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2819..2893
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2894..2977
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     exon            2978..8457
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6551..6556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /note="hexamer: AATAAA"
     polyA_site      6572
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /note="major polyA site"
     regulatory      8433..8438
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /note="hexamer: AATAAA"
     polyA_site      8457
                     /gene="Pde11a"
                     /gene_synonym="6330414F14Rik; A630086N24Rik; Gm350;
                     PDE11A1"
                     /note="major polyA site"
ORIGIN      
        1 agtggcggtg gagcagcgac tggctgcggg agccggagca gaggtggcgc gagccgcgcg
       61 gggaagcagc ggtggccgcg gcaggcacct gtgcggagca ggaggagcag ggctagggtg
      121 ggtggcagct gccgctctga gccgcggaag gagcaggtat ctcccaactg ggatcctgct
      181 gttcctgtca acctccggct gcctggcagg aaggtagtgc cgagctgggg acactggtgt
      241 gggcaaacat gtgcagcaac aactagaggc ctcgggacag gttaacgtgt ggttccagag
      301 tagagaggag aagcaagggg tgccctggac catggcagcc tcccgcctgg acttcgggga
      361 ggtggaaact ttcctggata ggcacccaga gttgtttgaa gattacttga tgcgaaaagg
      421 aaaacaggaa ctggtggaca agtggctaca aaggcacact tctggacagg gggcttcaag
      481 cctgaggcct gccctggctg gcgctagcag cttggctcag agcaatgcta aaggcagccc
      541 tggcataggt ggtggtgctg gaccccaggg ctctgcccac agccatccca cgcccggtgg
      601 tggggaaagt gctggggttc ccttgagtcc cagttgggcc agtggcagcc ggggtgatgg
      661 cagcctgcag cggagagctt cgcagaaaga gctgaggaag agctttgccc gctccaaagc
      721 catccatgtg aacaggacct acgatgaaca ggtgacctcc cgggcccagg agcccctgag
      781 cagtgtccgg aggagagccc tgctcagaaa ggccagctct ctgcccccca ccacagctca
      841 cattctaagt gccctgctgg aatcgagggt gaatctgcct cagtatcccc ctacggccat
      901 cgactacaaa tgccacctca aaaagcataa cgagcgccag tttttcctgg aactggtcaa
      961 ggacatctcc aatgaccttg acctcaccag cctaagctac aagatcctca tctttgtctg
     1021 tctcatggtg gacgctgacc gttgctctct cttcttagtg gaaggggcag ctgctggcaa
     1081 gaagacgttg gtctccaagt tttttgatgt gcacgcagga accccactgc tcccctgcag
     1141 tagcacagag aactcaaatg aggtgcaagt cccctggggc aaaggtatca ttggctatgt
     1201 cggggaacac ggagaaactg ttaacatccc ggatgcctac caggaccgaa gattcaatga
     1261 tgaaattgac aagctgactg gatacaagac aaaatcacta ttgtgcatgc ccattcggaa
     1321 cagcgacggg gagattatcg gtgtggccca ggcgataaat aaggtccctg agggtgctcc
     1381 atttacagaa gatgacgaaa aagttatgca aatgtatctt ccgttctgtg gaatcgccat
     1441 atctaatgct cagctcttcg ctgcctcaag gaaagaatac gaaagaagca gggccctgct
     1501 ggaggtggtc aatgacctct ttgaagaaca gactgatctg gaaaagattg tcaagaaaat
     1561 aatgcatcgg gcccaaactc tgctgaaatg tgaacgctgt tccgtcttac ttctagaaga
     1621 cattgagtcc ccagtggtga agtttaccaa atcctttgaa ctgatgtccc caaagtgcag
     1681 cgctgatgct gagaacagtt tcaaagagag tgtggagaaa tcgtcttact ctgactggct
     1741 gataaacaac agcatcgctg agctggttgc ttcgacaggc cttcctgtga atgtcagcga
     1801 tgcctaccag gacccacgct ttgacgctga ggctgaccag atatctggtt ttcatataag
     1861 atctgttctc tgtgtcccca tttggaacag caaccaccaa ataattgggg tggctcaagt
     1921 gttgaacaga ctcgacggaa aaccttttga tgatgcagac caaaggcttt ttgaggcctt
     1981 tgtcatcttt tgtggccttg gtattaacaa cacaattatg tacgatcaag tgaagaagtc
     2041 ctgggccaag cagtctgtgg cccttgatgt gctgtcgtac cacgccacgt gttccaaggc
     2101 tgaagtcgac aagtttaagg cagccaacat ccctctggtg tcggaactgg ccatcgatga
     2161 catccatttc gatgactttt cccttgatgt tgatgccatg atcacagccg ctctacggat
     2221 gttcatggag ctggggatgg tacagaaatt taaaatcgac tatgagaccc tgtgcaggtg
     2281 gcttctgacg gtaaggaaaa actatcggat ggttctctac cacaactgga gacatgcctt
     2341 caacgtgtgc caactgatgt ttgccatgct aactactgct gggttccaag agattctgac
     2401 cgaggtggaa attttagcgg tgattgtggg atgcctgtgt catgacctcg accacagggg
     2461 aaccaacaat gccttccaag ctaagagtga ctctgcactg gcccagctct acgggacctc
     2521 ggcgaccttg gagcatcacc attttaacca tgctgtgatg atccttcaga gtgagggtca
     2581 caacatcttt gctaatttgt cctccaagga atacagtgac ctcatgcagc tcctgaagca
     2641 gtcaatatta gccacagacc tcacgctcta ctttgagaga agaactgagt tcttcgagct
     2701 tgtcaggaaa ggagactacg attggagcat cacaagccac cgagatgtgt tccgatcaat
     2761 gttaatgaca gcctgtgacc ttggagccgt gaccaaaccg tgggagatct ccagacaggt
     2821 ggctgaactt gtaaccagcg agttctttga acaaggagat cgggagaggt cggaactcaa
     2881 gctcactccc tctgctattt ttgaccggaa ccggaaagat gaactgcctc ggttgcagct
     2941 ggagtggatt gatagcatct gcatgccttt gtatcaggcc ttggtgaagg tcaatgcaaa
     3001 actgaaacca atgctggatt cagtggctgc caaccgaagg aagtgggaag agttgcacca
     3061 aaaaagacta caggtctcgg ctgcctcccc agaccctgcc agtcccatgg tggccgggga
     3121 agacagactg taaaccaccc agagccgctg cgccaccctg tggtcgggag ggtcctccgc
     3181 atcctgaact acttgctttg gttgaaccgc aacatcctat tggttcagct cggatagtca
     3241 gtcccaaagc tttgattgta tcaggaagca cccaagaaag tgtcgttggc agcagaggca
     3301 cgaaagatgg agtgggacag agaatatgac cctcaacttt ccatcctgaa cccatactgc
     3361 tggatttgga tccatggacc tgagggccat cagccccact cctgctgagc tgagaggaca
     3421 ccgtggtaaa agtgtcacta tggctgcttc ctgtatggta cttttctgtt atgagccctt
     3481 cctttccttg cagcctctcc tggagcaggc tttctcacca gatggtcccg agaatgcaga
     3541 tttgcgtttt agatgtttta cttctaatcc tactgcttct tagtgatgca ccaggccctt
     3601 gattctcatt gcctagtgga taaacctacg tgacttaata gtgccaggta accccagtct
     3661 tgattcccac agtagtagta tagtagggtg tgtttgtcta attgatgtta cacagagtct
     3721 gtaactctgc agtgagatag atcatggttc actttttgaa ttcagccttt tgtaagcaaa
     3781 gcctttttct aggaggaaca gattaataat tttattaaag caaaggtgaa aggggatcta
     3841 aggccacatg taccacgaat gacatgtata aataccggaa ctgcttccaa cagtgagaaa
     3901 tagttctcag gtgcagaggc acccagtgac caagcatgga atagtttgaa gtgaagggga
     3961 tgaaggtttg aggatacaat ttaaggaacc acgattttac attttccctt acatgtaaaa
     4021 aagaatattc tagaaaggcc aggaagtaac atctgtacaa cctatttcca ctaggtctat
     4081 agcatgtgaa aacaagcagt tttaaataga tgcggtcact agaacactgc tttgatgccc
     4141 agttcacctg agacactaaa gacaggatct ctcttaccca aagaacactg ggcttgctgc
     4201 ctctcgcttt gcatcattag ctgagtgagt tctgactaga cttctctgtt tcccaaagtg
     4261 ccaagtgaga ttacactagg gatttatttg caggaatcct agcactgaaa tcctttgtcc
     4321 aaataaactt ttaaggattc cctagggcaa aagagagctg gaatctgaaa acttctgtgt
     4381 gaagcaggtc ttgggcatcc agagcccaga caagcatctc tttgttcacg tacatggtgc
     4441 tccacagaga gattcagaga gccgggaagt ccctggcggt gtaaaacctg agagtcaacc
     4501 cagctctgaa caatctaaaa cttaaggaaa cttttctcct caacttacaa aacatttttt
     4561 tccttagttt aatggatcag tatcggtttt taactttact caaatccaaa agaaacaggg
     4621 gtatagtttc acagggtcgt tgccttctgg gtattaagga gatatttgaa ggtatatggg
     4681 gtgggggtgg gggatagtta aatgaagata acaagacagg aaaggagtga gctgaaaatc
     4741 atccaaggaa ggcagctgtg ttatagcaag aaacattata aaccatgcaa aaggtggggc
     4801 tagaaaaaga gaatagagag aagactacag gagaattagg ggcagaggca gatggttaag
     4861 acaaaccggc taaggagtgg tgaaagtcaa tccaaacaaa gaaaaatcat tgcacttcca
     4921 gaccaaaaaa aaaaaaaaaa aaaaaaaaaa gagggtgcag aaccttgttg tcaattgagt
     4981 aggaaagaga caattacaca gggaggggct tgacaaagga gagaagaaag agatcagtgc
     5041 actaagaaac aatgttgcta ggtgcccttt acagctgtgt ctgcagggaa gactggagag
     5101 actgcagcgg aaaatgagct caggaaaaaa aaagaaaaaa aaaagcttcg gcagagcagg
     5161 taagaggagg ctgttcagat ttatggattt tattaagcca gttttctgaa tcactctgct
     5221 ataaaggaaa atagcttctg cagagctgtc tcccctcctt caggtgaaga acaaaggcta
     5281 ggcggagcta ggaacagacg tggggggggg ggggagtctg caagatcatt gtgtctgcag
     5341 cccaggtgct tttgggtact gcaccacagc acctcatcac ggtttatatc aaagatagga
     5401 atcgagaatc agtccatatg aggttgcagt gagcctgcat ctgttaccct tccaaacaat
     5461 aaataaacag ccagaaaaac ggatcccagt ccatctacag caagaaattt caagcatgta
     5521 ctttatgata atactggatc ccgggtcttt cagagactgc cggcagcaac gagacagggt
     5581 cccctccacc ctgaatgtcg atgcttcaga gtgctgaggg ggacataatt tatagcacaa
     5641 accctacagc ttccactaag gttgagtttt cctctcctct tcttgccttt ttattggctt
     5701 tactgcatgg ttgccaatag caacatccac tggtagcaaa gggagttcaa caccattgca
     5761 atctgccaga cagtagaggc ctgacaatgt caaccatcaa tcaaatagct gtttgtaatt
     5821 gcttccatct atttcccctg tctcaaggta tctttcccat tgtgggtcaa gattcagaaa
     5881 ctgctgacta atgtcccggg ttggttcctc catgactatg gagaagttca agagagggcg
     5941 gtagcccact tgatgtcgaa ccctggagct taaaggttgg gattctaggg cttgaaggta
     6001 tttttaaatc ccctgcaaag caatttcttt cccatgtacc tgagtcatgt ccgatctcta
     6061 tccaaagcac agagagaggg gacttcccaa gccttattta gcactgttgt ttcttgctga
     6121 aggaaggcat ctgaaatatg agccttcttc aaaataagac tcgaacagac aagattgcct
     6181 ccttcctcgg gtctttaggc atgaacagct gagatgaggc catcctactt ttgtatgaga
     6241 tgtatttttt cagaatgttt attcgtactg gtgagtatgt tgctaatccg cacacaagct
     6301 accgcacagc tggagatcgt ggtgagcaag ggcgcctggg cggccagcca agggtgccta
     6361 ccagtccaca gcagggcagg actccatcct agctggctgc ccatgggcct ctcgttttgg
     6421 gattctgaac ctctgcaggt ttctatctgt taaataaaag caccaacttt tattcagttt
     6481 ctgcttcaaa ggaccaagct atgctttgct ttggaaaatt catccagggt cattttcttt
     6541 ctacttctca aataaaatag aagcaaacct gagagagcct gagagccatc ttctcactga
     6601 aagatgcaaa aaaaaaaaaa aaaatgtatg catagaggga gcagacacag aacaaccttc
     6661 ctacaaagtg tctgagcctg cttctaatga ccgtgaatga tgtagccatc agtacctgct
     6721 tgtaaccgtg ttctcttagt tcataaagaa ccgttttcag ttatctcaca gcattgcatc
     6781 cgtatgggaa tcattagttt agaagggaat ttataaagag aaccaagaaa agtaaaaatg
     6841 gcacatgcac ttcctgtttc tgaaacctga aggcctacat gcttcccagg acttcgggtt
     6901 tgcaagtatc ttggatttaa aacgatctta gaattttttt tttattagtg caatgacagt
     6961 aatatgtttc tggaattcca ctgccttctc tgtatgtcag agtttcgctt attttagtca
     7021 agtcattagg ctgactttac tcaatctact ttgtagaaca attattttta aaacccagtt
     7081 ctcttgctga ccctggtgag ttacagaaag gcacacacac acacacacac taatcatcgt
     7141 ttatagtaat agaaacaatg caaccaaaat ctaaatatgt ttggaataaa tccaaatata
     7201 aagaaaaatg cattggcatg ggcccgggga agacaaaaag attaacaaca gtaactgaat
     7261 gtaacctaat ttgggacctt aagagctctg tcacgcctcc tttagtgaat tctgaatgtt
     7321 tcatagcagc aaactgggac tgtttttctg ccatgtgctg tcaagagctc ccacacccca
     7381 attatactag gagtttagag gttaagtctg gctattagtg gccaaagtat cacacacttc
     7441 ctaagtcagc agctctcaac ctgtgtgtgg agatcccttt gggggtcaca catcaggcgt
     7501 cctacatagc agatatatat ttacattaaa attcatagca ggagaaagtt acaatgctga
     7561 agtggcaatg agggtaattg tatggttggg ggccacgaca ccatgaggaa ctgtgttaaa
     7621 gggtcgcagc gttagtaagg ttgagagcca ctgcgctaac tgaacaataa ctagaacaca
     7681 aacagaaata aaccaggctg tgtctggaga atttcatggg gggggagggg gggcattaga
     7741 tttagcagca cccagtgctg tgttctcctt gtccatgcac ccctgcccct gcctagtgat
     7801 ttggggttgt acagactttg atttttgaat gtccttgcat gtctgtgcat ctctcagcag
     7861 cactaagggc aaactgttca tctgtgtcag tctgtaccaa ccaatgtctc tcaatgtaca
     7921 ctctgctgca aaagtgttct atgaagtaaa ttaactattt gctaaaaaaa aaaaaaaaaa
     7981 aaaaaaaaaa aaaaacccaa gtgtgttctt tgagaccatc gtttgggtga gttcccttct
     8041 gtgtctatgt gtgtgttaga gtgctcattt ttcttttgct ctagcgtaga tttagatgag
     8101 atcatattta cagcagtttt aaagcatagc gcaagtgtgg agatagtagt ctagttattg
     8161 agtctcgttc cctcggaggg ctgttacttt tgctattttc ttttgattta caacagttta
     8221 aaaaggattc cctactgact tgcttcctaa ttactgttgc tttacacaga agaaggagaa
     8281 gacgcgctcc tctgtttgtg tcctttccag gctaatcatt gtaggaacac ttggtttaat
     8341 gctgcttgtc catttcagaa gcaactacac gtggctttga aacgggctac ttaaaaaaaa
     8401 aaaaaaaaaa aaaaaagaga cttcttgttt ttaataaaat gcttaatggt taactaa
//
