LOCUS       NP_035182                869 aa            linear   ROD 30-APR-2024
DEFINITION  programmed cell death 6-interacting protein isoform 3 [Mus
            musculus].
ACCESSION   NP_035182
VERSION     NP_035182.2
DBSOURCE    REFSEQ: accession NM_011052.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 869)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 869)
  AUTHORS   Wang,Y.Z., Castillon,C.C.M., Gebis,K.K., Bartom,E.T., d'Azzo,A.,
            Contractor,A. and Savas,J.N.
  TITLE     Notch receptor-ligand binding facilitates extracellular
            vesicle-mediated neuron-to-neuron communication
  JOURNAL   Cell Rep 43 (2), 113680 (2024)
   PUBMED   38241148
REFERENCE   3  (residues 1 to 869)
  AUTHORS   Hermosilla Aguayo,V., Martin,P., Tian,N., Zheng,J., Aho,R., Losa,M.
            and Selleri,L.
  TITLE     ESCRT-dependent control of craniofacial morphogenesis with
            concomitant perturbation of NOTCH signaling
  JOURNAL   Dev Biol 503, 25-42 (2023)
   PUBMED   37573008
REFERENCE   4  (residues 1 to 869)
  AUTHORS   Laporte,M.H., Chi,K.I., Caudal,L.C., Zhao,N., Schwarz,Y.,
            Rolland,M., Martinez-Hernandez,J., Martineau,M., Chatellard,C.,
            Denarier,E., Mercier,V., Lemaitre,F., Blot,B., Moutaux,E.,
            Cazorla,M., Perrais,D., Lante,F., Bruns,D., Fraboulet,S.,
            Hemming,F.J., Kirchhoff,F. and Sadoul,R.
  TITLE     Alix is required for activity-dependent bulk endocytosis at brain
            synapses
  JOURNAL   PLoS Biol 20 (6), e3001659 (2022)
   PUBMED   35658004
  REMARK    GeneRIF: Alix is required for activity-dependent bulk endocytosis
            at brain synapses.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 869)
  AUTHORS   Jin,Z.L., Yao,X.R., Wen,L., Hao,G., Kwon,J.W., Hao,J. and Kim,N.H.
  TITLE     AIP1 and Cofilin control the actin dynamics to modulate the
            asymmetric division and cytokinesis in mouse oocytes
  JOURNAL   FASEB J 34 (8), 11292-11306 (2020)
   PUBMED   32602619
  REMARK    GeneRIF: AIP1 and Cofilin control the actin dynamics to modulate
            the asymmetric division and cytokinesis in mouse oocytes.
REFERENCE   6  (residues 1 to 869)
  AUTHORS   Chatellard-Causse,C., Blot,B., Cristina,N., Torch,S., Missotten,M.
            and Sadoul,R.
  TITLE     Alix (ALG-2-interacting protein X), a protein involved in
            apoptosis, binds to endophilins and induces cytoplasmic
            vacuolization
  JOURNAL   J Biol Chem 277 (32), 29108-29115 (2002)
   PUBMED   12034747
  REMARK    GeneRIF: overexpression of Alix-CT leads to cytoplasmic
            vacuolization into tubulo-vesicular structures
REFERENCE   7  (residues 1 to 869)
  AUTHORS   Aubry,L., Mattei,S., Blot,B., Sadoul,R., Satre,M. and Klein,G.
  TITLE     Biochemical characterization of two analogues of the
            apoptosis-linked gene 2 protein in Dictyostelium discoideum and
            interaction with a physiological partner in mammals, murine Alix
  JOURNAL   J Biol Chem 277 (24), 21947-21954 (2002)
   PUBMED   11927596
  REMARK    GeneRIF: Biochemical characterization of two analogues of the
            apoptosis-linked gene 2 protein in Dictyostelium discoideum and
            interaction with murine Alix.
REFERENCE   8  (residues 1 to 869)
  AUTHORS   Chen,B., Borinstein,S.C., Gillis,J., Sykes,V.W. and Bogler,O.
  TITLE     The glioma-associated protein SETA interacts with AIP1/Alix and
            ALG-2 and modulates apoptosis in astrocytes
  JOURNAL   J Biol Chem 275 (25), 19275-19281 (2000)
   PUBMED   10858458
REFERENCE   9  (residues 1 to 869)
  AUTHORS   Missotten,M., Nichols,A., Rieger,K. and Sadoul,R.
  TITLE     Alix, a novel mouse protein undergoing calcium-dependent
            interaction with the apoptosis-linked-gene 2 (ALG-2) protein
  JOURNAL   Cell Death Differ 6 (2), 124-129 (1999)
   PUBMED   10200558
REFERENCE   10 (residues 1 to 869)
  AUTHORS   Vito,P., Pellegrini,L., Guiet,C. and D'Adamio,L.
  TITLE     Cloning of AIP1, a novel protein that associates with the
            apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction
  JOURNAL   J Biol Chem 274 (3), 1533-1540 (1999)
   PUBMED   9880530
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167246.2, AK167574.1, BE623112.1, AK029827.1, AK157704.1 and
            BM899382.1.
            
            On Sep 15, 2009 this sequence version replaced NP_035182.1.
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1. This
            results in a shorter protein (isoform 3), compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK169704.1, AK129340.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 64.03 cM"
     Protein         1..869
                     /product="programmed cell death 6-interacting protein
                     isoform 3"
                     /note="Alg2-interacting protein X; Alg2-interacting
                     protein 1; E2f1-inducible protein; ALG-2-interacting
                     protein 1; ALG-2-interacting protein X"
                     /calculated_mol_wt=95893
     Region          2..345
                     /region_name="BRO1_Alix"
                     /note="Protein-interacting, N-terminal, Bro1-like domain
                     of mammalian Alix and related domains; cd09240"
                     /db_xref="CDD:185763"
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylalanine.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4; propagated from
                     UniProtKB/Swiss-Prot (Q9WU78.3)"
     Site            order(131,134,147,150..151,199,202..203,212,215..216,335,
                     337,339,341)
                     /site_type="other"
                     /note="putative ESCRT-III binding site"
                     /db_xref="CDD:185763"
     Region          176..503
                     /region_name="Interaction with CHMP4A, CHMP4B and CHMP4C.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WU78.3)"
     Site            215
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4; propagated from
                     UniProtKB/Swiss-Prot (Q9WU78.3)"
     Region          360..698
                     /region_name="V_Alix"
                     /note="Middle V-domain of mammalian Alix and related
                     domains are dimerization and protein interaction modules;
                     cd09235"
                     /db_xref="CDD:185748"
     Region          383..869
                     /region_name="Interaction with SDCBP.
                     /evidence=ECO:0000269|PubMed:22660413"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WU78.3)"
     Site            order(440,498,502,505..506,669,672..673,676,679)
                     /site_type="other"
                     /note="YPXnL-motif binding site"
                     /db_xref="CDD:185748"
     Site            479
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4; propagated from
                     UniProtKB/Swiss-Prot (Q9WU78.3)"
     Site            481
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4; propagated from
                     UniProtKB/Swiss-Prot (Q9WU78.3)"
     Region          503..869
                     /region_name="Self-association.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WU78.3)"
     Site            638..644
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:185748"
     Region          715..808
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WU78.3)"
     Region          717..720
                     /region_name="Interaction with TSG101.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WU78.3)"
     Site            730
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4; propagated from
                     UniProtKB/Swiss-Prot (Q9WU78.3)"
     Site            741
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4; propagated from
                     UniProtKB/Swiss-Prot (Q9WU78.3)"
     Site            745
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4; propagated from
                     UniProtKB/Swiss-Prot (Q9WU78.3)"
     Region          798..807
                     /region_name="Interaction with CEP55.
                     /evidence=ECO:0000250|UniProtKB:Q8WUM4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WU78.3)"
     Region          <815..>869
                     /region_name="Amelogenin"
                     /note="Amelogenins, cell adhesion proteins, play a role in
                     the biomineralisation of teeth; smart00818"
                     /db_xref="CDD:197891"
     Region          838..869
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9WU78.3)"
     CDS             1..869
                     /gene="Pdcd6ip"
                     /gene_synonym="Aip1; Alix; Eig2; mKIAA1375"
                     /coded_by="NM_011052.2:153..2762"
                     /note="isoform 3 is encoded by transcript variant 3"
                     /db_xref="CCDS:CCDS23588.1"
                     /db_xref="GeneID:18571"
                     /db_xref="MGI:MGI:1333753"
ORIGIN      
        1 masfiwvqlk ktsevdlakp lvkfiqqtyp sggeeqaqyc raaeelsklr rsalgrpldk
       61 hegaletllr yydqicsiep kfpfsenqic ltftwkdafd kgslfggsvk lalaslgyek
      121 scvlfncaal asqiaaeqnl dndeglktaa kqyqfasgaf lhikdtvlsa lsreptvdis
      181 pdtvgtlsli mlaqaqevff lkatrdkmkd aiiaklanqa adyfgdafkq cqykdtlpke
      241 vfptlaakqc imqanaeyhq silakqqkkf geeiarlqha aeliknvasr ydeyvnvkdf
      301 sdkinralta akkdndfiyh drvpdlkdld pigkatlvkp tpvnvpvsqk ftdlfekmvp
      361 vsvqqslavf sqrkadlvnr siaqmreatt langvlasln lpaaiedvsg dtvpqsiltk
      421 stsvveqggi qtvdqlikel pellqrnrei leeslrllde eeatdndlra kfkdrwqrtp
      481 sndlykplra egakfravld kavqadgqvk eryqshrdti allckpepel naaipsanpa
      541 ktmqgsevvs vlksllsnld eikkeresle ndlksvnfdm tskfltalaq dgvineeals
      601 vteldriygg ltskvqeslk kqegllkniq vshqefskmk qsnneanlre evlknlatay
      661 dnfvelvanl kegtkfynel teilvrfqnk csdivfarkt erdellkdlq qsiarepsap
      721 sipppayqss paaghaaapp tpaprtmppa kpqpparppp pvlpanrvpp asaaaapagv
      781 gtasaappqt pgsapppqaq gppyptypgy pgycqmpmpm gynpyaygqy nmpyppvyhq
      841 spgqapypgp qqptypfpqp pqqsyypqq
//
