LOCUS       NM_024229               1915 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus phosphate cytidylyltransferase 2, ethanolamine
            (Pcyt2), transcript variant 1, mRNA.
ACCESSION   NM_024229
VERSION     NM_024229.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Cikes,D., Elsayad,K., Sezgin,E., Koitai,E., Torma,F., Orthofer,M.,
            Yarwood,R., Heinz,L.X., Sedlyarov,V., Miranda,N.D., Taylor,A.,
            Grapentine,S., Al-Murshedi,F., Abot,A., Weidinger,A.,
            Kutchukian,C., Sanchez,C., Cronin,S.J.F., Novatchkova,M.,
            Kavirayani,A., Schuetz,T., Haubner,B., Haas,L., Hagelkruys,A.,
            Jackowski,S., Kozlov,A.V., Jacquemond,V., Knauf,C.,
            Superti-Furga,G., Rullman,E., Gustafsson,T., McDermot,J., Lowe,M.,
            Radak,Z., Chamberlain,J.S., Bakovic,M., Banka,S. and Penninger,J.M.
  TITLE     PCYT2-regulated lipid biosynthesis is critical to muscle health and
            ageing
  JOURNAL   Nat Metab 5 (3), 495-515 (2023)
   PUBMED   36941451
  REMARK    GeneRIF: PCYT2-regulated lipid biosynthesis is critical to muscle
            health and ageing.
            Erratum:[Nat Metab. 2023 Apr;5(4):711. doi:
            10.1038/s42255-023-00791-1.. PMID: 37024756]
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Wei,Z., Yang,C., Feng,K., Guo,S., Huang,Z., Wang,Y. and Jian,C.
  TITLE     p75NTR enhances cognitive dysfunction in a mouse Alzheimer's
            disease model by inhibiting microRNA-210-3p-mediated PCYT2 through
            activation of NF-kappaB
  JOURNAL   Int J Biol Macromol 225, 404-415 (2023)
   PUBMED   36379282
  REMARK    GeneRIF: p75NTR enhances cognitive dysfunction in a mouse
            Alzheimer's disease model by inhibiting microRNA-210-3p-mediated
            PCYT2 through activation of NF-kappaB.
REFERENCE   3  (bases 1 to 1915)
  AUTHORS   Grapentine,S., Singh,R.K., Basu,P., Sivanesan,S., Mattos,G.,
            Oresajo,O., Cheema,J., Demeke,W., Dolinsky,V.W. and Bakovic,M.
  TITLE     Pcyt2 deficiency causes age-dependant development of nonalcoholic
            steatohepatitis and insulin resistance that could be attenuated
            with phosphoethanolamine
  JOURNAL   Sci Rep 12 (1), 1048 (2022)
   PUBMED   35058529
  REMARK    GeneRIF: Pcyt2 deficiency causes age-dependant development of
            nonalcoholic steatohepatitis and insulin resistance that could be
            attenuated with phosphoethanolamine.
            Erratum:[Sci Rep. 2023 Jun 22;13(1):10175. doi:
            10.1038/s41598-023-36725-w.. PMID: 37349480]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1915)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1915)
  AUTHORS   Fu,G., Guy,C.S., Chapman,N.M., Palacios,G., Wei,J., Zhou,P.,
            Long,L., Wang,Y.D., Qian,C., Dhungana,Y., Huang,H., Kc,A., Shi,H.,
            Rankin,S., Brown,S.A., Johnson,A., Wakefield,R., Robinson,C.G.,
            Liu,X., Sheyn,A., Yu,J., Jackowski,S. and Chi,H.
  TITLE     Metabolic control of TFH cells and humoral immunity by
            phosphatidylethanolamine
  JOURNAL   Nature 595 (7869), 724-729 (2021)
   PUBMED   34234346
REFERENCE   6  (bases 1 to 1915)
  AUTHORS   Tie,A. and Bakovic,M.
  TITLE     Alternative splicing of CTP:phosphoethanolamine
            cytidylyltransferase produces two isoforms that differ in catalytic
            properties
  JOURNAL   J Lipid Res 48 (10), 2172-2181 (2007)
   PUBMED   17646670
  REMARK    GeneRIF: alternative splicing of the mPcyt2 transcript is
            ubiquitous but could also be regulated in a tissue-specific manner,
            producing a variable ratio of mPcyt2alpha/mPcyt2beta mRNAs
REFERENCE   7  (bases 1 to 1915)
  AUTHORS   Fullerton,M.D., Hakimuddin,F. and Bakovic,M.
  TITLE     Developmental and metabolic effects of disruption of the mouse
            CTP:phosphoethanolamine cytidylyltransferase gene (Pcyt2)
  JOURNAL   Mol Cell Biol 27 (9), 3327-3336 (2007)
   PUBMED   17325045
  REMARK    GeneRIF: single Pcyt2 allele in heterozygotes can maintain
            phospholipid homeostasis
REFERENCE   8  (bases 1 to 1915)
  AUTHORS   Steenbergen,R., Nanowski,T.S., Beigneux,A., Kulinski,A., Young,S.G.
            and Vance,J.E.
  TITLE     Disruption of the phosphatidylserine decarboxylase gene in mice
            causes embryonic lethality and mitochondrial defects
  JOURNAL   J Biol Chem 280 (48), 40032-40040 (2005)
   PUBMED   16192276
REFERENCE   9  (bases 1 to 1915)
  AUTHORS   Poloumienko,A., Cote,A., Quee,A.T., Zhu,L. and Bakovic,M.
  TITLE     Genomic organization and differential splicing of the mouse and
            human Pcyt2 genes
  JOURNAL   Gene 325, 145-155 (2004)
   PUBMED   14697519
REFERENCE   10 (bases 1 to 1915)
  AUTHORS   Chiellini,C., Costa,M., Novelli,S.E., Amri,E.Z., Benzi,L.,
            Bertacca,A., Cohen,P., Del Prato,S., Friedman,J.M. and Maffei,M.
  TITLE     Identification of cathepsin K as a novel marker of adiposity in
            white adipose tissue
  JOURNAL   J Cell Physiol 195 (2), 309-321 (2003)
   PUBMED   12652657
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL663030.12 and AK140458.1.
            
            On May 4, 2018 this sequence version replaced NM_024229.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK140458.1, BC008276.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AL663030.12        73745-73759         c
            16-1912             AK140458.1         1-1897
            1913-1915           AL663030.12        65876-65878         c
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 84.33 cM"
     gene            1..1915
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /note="phosphate cytidylyltransferase 2, ethanolamine"
                     /db_xref="GeneID:68671"
                     /db_xref="MGI:MGI:1915921"
     exon            1..182
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     CDS             94..1308
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /EC_number="2.7.7.14"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ethanolamine-phosphate cytidylyltransferase;
                     phosphorylethanolamine transferase;
                     CTP:phosphoethanolamine cytidylyltransferase"
                     /codon_start=1
                     /product="ethanolamine-phosphate cytidylyltransferase
                     isoform 1"
                     /protein_id="NP_077191.2"
                     /db_xref="CCDS:CCDS25747.1"
                     /db_xref="GeneID:68671"
                     /db_xref="MGI:MGI:1915921"
                     /translation="MIRNGHGAASAAGLKGPGDQRIVRVWCDGCYDMVHYGHSNQLRQ
                     ARAMGDYLIVGVHTDEEIAKHKGPPVFTQEERYKMVQAIKWVDEVVPAAPYVTTLETL
                     DKHNCDFCVHGNDITLTVDGRDTYEEVKQAGRYRECKRTQGVSTTDLVGRMLLVTKAH
                     HSSQEMSSEYREYADSFGKPPHPTPAGDTLSSEVSSQCPGGQSPWTGVSQFLQTSQKI
                     IQFASGKEPQPGETVIYVAGAFDLFHIGHVDFLQEVHKLAKRPYVIAGLHFDQEVNRY
                     KGKNYPIMNLHERTLSVLACRYVSEVVIGAPYSVTAELLNHFKVDLVCHGKTEIVPDR
                     DGSDPYQEPKRRGIFYQIDSGSDLTTDLIVQRIIKNRLEYEARNQKKEAKELAFLEAT
                     KQQEAPPGGEID"
     exon            183..271
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            272..433
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            434..500
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            501..585
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            586..630
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            631..684
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            685..823
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            824..906
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            907..984
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1050
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1116
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1205
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1915
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1870..1875
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /note="hexamer: AATAAA"
     polyA_site      1890
                     /gene="Pcyt2"
                     /gene_synonym="1110033E03Rik; ET"
                     /note="major polyA site"
ORIGIN      
        1 gagatccgcg tgagcacgcg caagcgtcga gggcggggcg acggtactgt gtgtcgcggg
       61 agccgccagg atttgcgggg ggcctccggt gccatgatcc ggaacgggca cggggctgcc
      121 agcgctgctg ggctgaaggg tccgggagat cagcgcatcg tgcgggtgtg gtgcgatggc
      181 tgctatgaca tggtgcatta tggccactcc aaccagctac ggcaggcacg ggccatgggg
      241 gactacctca tcgtgggtgt gcatacggat gaggagattg ccaagcataa ggggcccccg
      301 gtgtttaccc aggaggagag gtacaagatg gtacaggcca tcaagtgggt ggatgaggtg
      361 gtgcccgctg ctccctacgt caccaccctg gagacactgg acaagcacaa ctgtgacttc
      421 tgtgttcacg gcaatgacat cacgctgaca gtagacggcc gagacaccta tgaggaagtg
      481 aagcaggctg ggaggtacag agagtgcaaa cgcacccagg gtgtgtccac cacagacctc
      541 gtgggtcgca tgctgctggt gaccaaggcc catcacagca gccaggagat gtcctctgag
      601 taccgggaat atgctgacag ttttggaaag ccccctcacc cgacacctgc cggggacaca
      661 ctttcctcag aagtctcctc ccagtgccct ggggggcaga gcccctggac aggggtgtcc
      721 cagtttctac agacatccca gaagatcatc cagtttgctt ctgggaagga gccccagccc
      781 ggggagacgg tcatctatgt ggctggtgcc tttgacctgt tccacatcgg gcacgtggac
      841 ttcctacagg aggtgcacaa gctagccaag aggccctacg tcatcgccgg cctacacttt
      901 gaccaggaag taaaccggta caagggcaag aactacccca tcatgaacct gcacgagcgg
      961 actctcagtg tgctggcctg ccggtatgtt tcagaagtgg tgattggggc accatactcc
     1021 gtgacagcgg agctcctgaa tcacttcaag gtggacctgg tgtgtcacgg gaagacagag
     1081 attgtacccg acagggatgg gtctgacccc taccaggagc ccaagagaag aggcatcttc
     1141 tatcagattg acagtggcag tgacctcact acagacctga ttgtgcagag gatcatcaag
     1201 aacaggctgg agtatgaagc acggaatcag aagaaagaag ccaaggaatt agcctttctg
     1261 gaggccacga agcagcagga ggcgccgcct ggaggggaga ttgactagct tcagacccgg
     1321 agatgttgtt cacgcccttg ccctgtgccc acctcttctc ttcctgcccg gctctgcttc
     1381 tgtgtcttga tgtcagctca cacaattcca aaggaaactg gccttgctga gggtgctgcc
     1441 tgcctgcgag gcacctgcct tgcagcaggc tctcagccct tccctctgag ctgctcagag
     1501 agggtagcta gcccaatgat gtggcccgtg gacaggataa gcagatgcac ctgtgactga
     1561 ctggaggcgt gttgctgtgt atcccgggca tccatgggtt ccaactgcca ctgccccagt
     1621 cttggccaga gatacccctc ctgcctgact ggaagctgca catctgccca ctgagctttg
     1681 gtgaaaggtc cagaggcttt ggggacctct gttcctgggc caccctgccc gtgggcaccc
     1741 tctaccttgg ggcacgttct agcaccccat tcctgactcc tggaagatgc acttgccccg
     1801 acagctgggc agcacggctg tcctctgcag agactgcctg gtcctcattg tactttggtg
     1861 gctcaactga ataaagcctt gtgggaagca ctctgtaggc tcccgacccc gggaa
//
