LOCUS       NM_009981               4787 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus phosphate cytidylyltransferase 1, choline, alpha
            isoform (Pcyt1a), transcript variant 2, mRNA.
ACCESSION   NM_009981
VERSION     NM_009981.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Wang,K., Xu,H., Zou,R., Zeng,G., Yuan,Y., Zhu,X., Zhao,X., Li,J.
            and Zhang,L.
  TITLE     PCYT1A deficiency disturbs fatty acid metabolism and induces
            ferroptosis in the mouse retina
  JOURNAL   BMC Biol 22 (1), 134 (2024)
   PUBMED   38858683
  REMARK    GeneRIF: PCYT1A deficiency disturbs fatty acid metabolism and
            induces ferroptosis in the mouse retina.
            Erratum:[BMC Biol. 2024 Jul 10;22(1):155. doi:
            10.1186/s12915-024-01954-6.. PMID: 38987738]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4787)
  AUTHORS   Fu,G., Guy,C.S., Chapman,N.M., Palacios,G., Wei,J., Zhou,P.,
            Long,L., Wang,Y.D., Qian,C., Dhungana,Y., Huang,H., Kc,A., Shi,H.,
            Rankin,S., Brown,S.A., Johnson,A., Wakefield,R., Robinson,C.G.,
            Liu,X., Sheyn,A., Yu,J., Jackowski,S. and Chi,H.
  TITLE     Metabolic control of TFH cells and humoral immunity by
            phosphatidylethanolamine
  JOURNAL   Nature 595 (7869), 724-729 (2021)
   PUBMED   34234346
REFERENCE   4  (bases 1 to 4787)
  AUTHORS   Kennelly,J.P., Carlin,S., Ju,T., van der Veen,J.N., Nelson,R.C.,
            Buteau,J., Thiesen,A., Richard,C., Willing,B.P. and Jacobs,R.L.
  TITLE     Intestinal Phospholipid Disequilibrium Initiates an ER Stress
            Response That Drives Goblet Cell Necroptosis and Spontaneous
            Colitis in Mice
  JOURNAL   Cell Mol Gastroenterol Hepatol 11 (4), 999-1021 (2021)
   PUBMED   33238221
  REMARK    GeneRIF: Intestinal Phospholipid Disequilibrium Initiates an ER
            Stress Response That Drives Goblet Cell Necroptosis and Spontaneous
            Colitis in Mice.
REFERENCE   5  (bases 1 to 4787)
  AUTHORS   Petkevicius,K., Virtue,S., Bidault,G., Jenkins,B., Cubuk,C.,
            Morgantini,C., Aouadi,M., Dopazo,J., Serlie,M.J., Koulman,A. and
            Vidal-Puig,A.
  TITLE     Accelerated phosphatidylcholine turnover in macrophages promotes
            adipose tissue inflammation in obesity
  JOURNAL   Elife 8, e47990 (2019)
   PUBMED   31418690
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4787)
  AUTHORS   Bakovic,M., Waite,K., Tang,W., Tabas,I. and Vance,D.E.
  TITLE     Transcriptional activation of the murine CTP:phosphocholine
            cytidylyltransferase gene (Ctpct): combined action of upstream
            stimulatory and inhibitory cis-acting elements
  JOURNAL   Biochim Biophys Acta 1438 (1), 147-165 (1999)
   PUBMED   10216289
REFERENCE   7  (bases 1 to 4787)
  AUTHORS   Moon,E.A. and O'Neill,C.
  TITLE     CTP:phosphocholine cytidylyltransferase activity in the
            preimplantation mouse embryo
  JOURNAL   J Reprod Fertil 110 (2), 213-218 (1997)
   PUBMED   9306973
REFERENCE   8  (bases 1 to 4787)
  AUTHORS   Tang,W., Keesler,G.A. and Tabas,I.
  TITLE     The structure of the gene for murine CTP:phosphocholine
            cytidylyltransferase, Ctpct. Relationship of exon structure to
            functional domains and identification of transcriptional start
            sites and potential upstream regulatory elements
  JOURNAL   J Biol Chem 272 (20), 13146-13151 (1997)
   PUBMED   9148929
REFERENCE   9  (bases 1 to 4787)
  AUTHORS   Kalmar,G.B., Kay,R.J., LaChance,A.C. and Cornell,R.B.
  TITLE     Primary structure and expression of a human CTP:phosphocholine
            cytidylyltransferase
  JOURNAL   Biochim Biophys Acta 1219 (2), 328-334 (1994)
   PUBMED   7918629
REFERENCE   10 (bases 1 to 4787)
  AUTHORS   Rutherford,M.S., Rock,C.O., Jenkins,N.A., Gilbert,D.J.,
            Tessner,T.G., Copeland,N.G. and Jackowski,S.
  TITLE     The gene for murine CTP:phosphocholine cytidylyltransferase (Ctpct)
            is located on mouse chromosome 16
  JOURNAL   Genomics 18 (3), 698-701 (1993)
   PUBMED   8307580
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB642590.1, AK076050.1 and AC087556.30.
            
            On Jul 10, 2009 this sequence version replaced NM_009981.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. All three variants encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.6242.1,
                                           SRR1660815.11039.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-111               BB642590.1         2-112
            112-1737            AK076050.1         37-1662
            1738-4787           AC087556.30        7209-10258          c
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 22.69 cM"
     gene            1..4787
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /note="phosphate cytidylyltransferase 1, choline, alpha
                     isoform"
                     /db_xref="GeneID:13026"
                     /db_xref="MGI:MGI:88557"
     exon            1..111
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            112..238
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..1225
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /EC_number="2.7.7.15"
                     /note="CT A; CCT A; CCT-alpha; phosphorylcholine
                     transferase A; CTP:phosphocholine cytidylyltransferase A;
                     CTP:phosphocholine cytidylyltransferase alpha; CTP
                     phosphocholine cytidylyl transferase"
                     /codon_start=1
                     /product="choline-phosphate cytidylyltransferase A"
                     /protein_id="NP_034111.1"
                     /db_xref="CCDS:CCDS28120.1"
                     /db_xref="GeneID:13026"
                     /db_xref="MGI:MGI:88557"
                     /translation="MDAQSSAKVNSRKRRKEAPGPNGATEEDGIPSKVQRCAVGLRQP
                     APFSDEIEVDFSKPYVRVTMEEACRGTPCERPVRVYADGIFDLFHSGHARALMQAKNL
                     FPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQHCRYVDEVVRNAPWTLTPEFLAE
                     HRIDFVAHDDIPYSSAGSDDVYKHIKDAGMFAPTQRTEGISTSDIITRIVRDYDVYAR
                     RNLQRGYTAKELNVSFINEKKYHLQERVDKVKKKVKDVEEKSKEFVQKVEEKSIDLIQ
                     KWEEKSREFIGSFLEMFGPEGALKHMLKEGKGRMLQAISPKQSPSSSPTHERSPSPSF
                     RWPFSGKTSPSSSPASLSRCRAVTCDISEDEED"
     exon            239..338
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            339..455
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            456..607
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            608..686
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            687..829
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            830..1018
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..4787
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4761..4766
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /note="hexamer: AATAAA"
     polyA_site      4780
                     /gene="Pcyt1a"
                     /gene_synonym="Cctalpha; CTalpha; Ctpct; Cttalpha"
                     /note="major polyA site"
ORIGIN      
        1 agtccggtca gatgtttccc gggcgtctcc ccgcaaccca tttgacttcg ctagtcggtg
       61 acgcggcgcg gggaagggat ccgaggggga ccggagcctg gaggagttga gttaaaagaa
      121 gatggatgca cagagttcag ctaaagtcaa ttcgaggaag aggaggaaag aggcacctgg
      181 ccctaatgga gcaacagagg aagatggaat tccttccaaa gtgcagcgtt gcgcagttgg
      241 tttacggcag ccagctcctt tttctgatga aattgaagtt gactttagta agccctatgt
      301 cagggtgact atggaagaag cctgcagggg aactccgtgt gagcggcctg tgagagttta
      361 tgcggatgga atatttgact tgtttcactc tggtcatgcc cgggctctga tgcaagcaaa
      421 gaaccttttc cctaatacgt atctaattgt gggagtctgc agtgatgagc taacgcacaa
      481 cttcaagggc ttcactgtga tgaacgagaa tgagcgctat gacgccgtgc agcactgccg
      541 ctacgtggac gaggtggtgc ggaacgcccc ctggactctg acgcctgagt tcctggcaga
      601 gcaccggatt gattttgtcg cccatgacga catcccctat tcttctgcag ggagcgatga
      661 tgtgtataag cacatcaagg acgcaggcat gtttgctccc acacagagga cagaaggtat
      721 ctccacatca gacatcatca cccgcattgt ccgtgactat gatgtgtatg caagacggaa
      781 cctacagagg ggctacactg ccaaggagct caatgtcagc tttatcaacg agaagaaata
      841 ccacttgcaa gaacgagttg ataaagtaaa gaagaaagtg aaagatgtgg aggaaaagtc
      901 aaaagagttt gtgcagaagg tggaagagaa gagcatcgac ctcatccaga agtgggagga
      961 gaagtcccga gagttcattg gaagtttcct ggaaatgttt ggtccagaag gagcactgaa
     1021 gcacatgctg aaggagggaa aaggtcggat gctgcaggcc atcagtccca agcagagccc
     1081 cagcagcagc cctactcatg agcgctcccc ctccccctcc tttcggtggc ccttctctgg
     1141 caagacttcc ccatcttcct ccccagcaag tctttctagg tgcagggctg tgacctgtga
     1201 catcagcgag gatgaagagg actaactttt catccctgtt ccctctctcc cccactgccc
     1261 atcacctcca gaagctctct gtcgaattcc attttgtgat cccaacacta aacctaagga
     1321 catctacaaa gaaaagacaa ccggggcaag aggacctggg aatggggaac caaaacagcc
     1381 catgccctga gacattctca gccacaccaa ggaggctgcc cataccttag aagcctgctc
     1441 tgcatccatc ttccctcccc agaactttgc tttactgttt atgctcatgt aatcttgaca
     1501 gggaaattgt catttttatc aatttttttt aaaaagtagt ctttcaaata tagtaagtag
     1561 aactaatttt ttgcccctga ggaatgggca gaagaaggaa gcgtggaatg cccagaggcc
     1621 ttttttcctg aacactagtg cttgccttct gttctgaagc aaagtggctt gccagatcct
     1681 tcaaacaggg agccatgcta atggccaatt tggggactat cataatttta acttccctaa
     1741 aaaaaaaaaa tctcaaagct gaattagctc cagattccct gagtgtcctg ccggcccatg
     1801 tgtcagcgtg gctgtgggag ggaagagatc tgagcctaac ttttcttact ccctgaggag
     1861 tttcttgttg gacccaatgg ggtccatgaa gacactgatg gggatgaaac caaaccacag
     1921 agcatctact gcagtttttc tgctgtgttc atcttctcag aaaatcctgt tgcccaatta
     1981 tttgggagct cttgtcaatc tctttcctgc accccacctg taaaggaagg gacattttga
     2041 gggctactgt gattggagag aggagtggga taaactcagt ctagtacaag ggcagttgag
     2101 gttgtttctg ctgggcatgc gttcctcagc ctgcagctgc cctctcagca ctgcgcccag
     2161 ctttgtacaa aggatgcttt gtaagtgttt gctgcttgcc cttgacctat tcattgatcc
     2221 gtgttgccta ttgccatatt cagcacttag actttcacag agcatgcagg caaaagcacc
     2281 aggaaacact gatctctgaa acactctcct caacctttgc tcgtcttaga atattcttgg
     2341 gtcaaaagag tatcacatgg agactctttg ctccaagagc tgcaccgagc agggacttcc
     2401 tccagtgctc ttccagaagc aacaggcatg ggttaaaact gctagcacac agaggtccat
     2461 gccctcttgt ctgcctgtgc ctggtggccc caagaggaaa cagaatttcc aatggaagag
     2521 tactgactct caacacttac tattgagcag gagctggtgc ttgcagtccc ctggcacccc
     2581 gtgtcactac atggcccagc acgttcatca ccatccatta tcctgtagct ggatttaggg
     2641 caaagttgtt tatgtccagg acctactagt tgtcactcat aaatacccct tgtgggggtg
     2701 gagctttctt gttttcaaca aagaaaacca cattttcttt ttccttgtac ctcctcactg
     2761 gtccatgctg ggactatgag catttacagt cctccctgga cactttctgg tggaagctgt
     2821 gctactgcct aagggtctga ggctaacacc acacagcgcc tgcacagcag aactacctag
     2881 gctcctaaac tatttcccag gcaaagctga gcagagaaga ggggcaaaga ggccccactc
     2941 gtgttccagt ggcccatgga aacgccattg ctaactaact cctcatgaag ggctgctttc
     3001 actcagatct gacccatatg gccttggcct ggaatccttt ctctgaacct gttgaaatta
     3061 gtcttttctc acattatgta ctgctgtgtg gctcacttta acttttctac tttagtcttc
     3121 tcttcgggga tccgtttctc tctgccttat tctcccaggt atacagaggt ggtgatgtca
     3181 ctgctggcac tcacctactt gttctctcag tgcatgtcac ccaggtgtac cctgtggaaa
     3241 tgcaaaggga gccgctcatg tgcgattttc ccccatgtag tcattcaagt ctactgtctg
     3301 ggtcccaaac atgagagcgt ctatgctgac aagatatgta acccagctag gactagccta
     3361 tgctcatatt caacctttac tatctcctaa cactctgact ctcagatagt ttagacataa
     3421 gcaaaaatgc tgtgggtgtg gctgcatgct aggtacttga gggtcagtgt gctcctcggt
     3481 gctagaaaaa ggtgctgctg acatagcgca ccgtcggcta cgtgtatcta gttaaatgtg
     3541 ggtaagctgt ataacgctgt tgctgtgact gtagtccaca cctcttgtgc aacctgggct
     3601 atagagctgt ttccccaagt ctacacgaaa gcaagcagca tggaagggcc aggagagccg
     3661 taagttgttt ggcctcatag cctctgtgcc ttaggaggag gcttgaagac aggagccctg
     3721 gagctggtat tgaggaggag ccagtgtgac agttaacctt ggcatggacg ggaggctgaa
     3781 aatagaggtt gccacttaga gactgtagaa atggagcacc agtgccatgg cagccactcc
     3841 tgggagctaa gcctgagacc ctaaagtgag gaaggggtgg agttgaggtc gagaatcctg
     3901 acaagaaaaa tactgagaga acctttctgg tcacttttct cagcagcacc ttagttattc
     3961 ttaaggccgc tcatctgacc agctgtctag gatgtccctc cacagtggct cttctaagtg
     4021 ctttgtcact gtctccagcc cacgttcgct tgtgtaactc acgttctgga gcctgttgtc
     4081 tttgaaatct gttctttatc aaccttttac tcctttccaa aatcgattct gtttcttgct
     4141 ttggtttaat ccccctaagc cataccttct agatttctgt cagcatatgc gtcgaaattg
     4201 aacccacaca gttcaaagaa ggcttcctta acaatgtttt aagactaacg gtattttttt
     4261 taaaacatac caatataagt attttaaaag gtatttttca aagtcataac aatgattgtt
     4321 ttctctcaga atagattatc atgggataaa gagtggtccc tagcttctat ttttcaaaat
     4381 aagtaggaca tgttcttgga cttaatctat taaaagttag ctctcctaaa agaaaaaaga
     4441 aataagtttg ccagaacttt ttgtcggcag gagattgggg tagagaacag aaatgccagg
     4501 attaacattt agatgaccca ggttgggttt cactaggttt gaagtgagct ccttccaccc
     4561 tattggagaa agtgactgtt ctgaacagtt agagttggtt ggctgagtgt tttgcatcaa
     4621 ggctcctgaa cagaaaacac tgttggaaag agtcacccat gacattacca gtgcaatggg
     4681 gagtttgcct ccgagttcag tgtctcacgt tactgtctaa tactttgtgc agaattgtat
     4741 tggcttttgt cattcagcca aataaaggcc atgtgttttt gcctggt
//
