LOCUS       NM_001162946            4148 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus pyruvate carboxylase (Pcx), transcript variant 1,
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001162946
VERSION     NM_001162946.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Deja,S., Fletcher,J.A., Kim,C.W., Kucejova,B., Fu,X., Mizerska,M.,
            Villegas,M., Pudelko-Malik,N., Browder,N., Inigo-Vollmer,M.,
            Menezes,C.J., Mishra,P., Berglund,E.D., Browning,J.D.,
            Thyfault,J.P., Young,J.D., Horton,J.D. and Burgess,S.C.
  TITLE     Hepatic malonyl-CoA synthesis restrains gluconeogenesis by
            suppressing fat oxidation, pyruvate carboxylation, and amino acid
            availability
  JOURNAL   Cell Metab 36 (5), 1088-1104 (2024)
   PUBMED   38447582
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4148)
  AUTHORS   Paredes,A., Justo-Mendez,R., Jimenez-Blasco,D., Nunez,V.,
            Calero,I., Villalba-Orero,M., Alegre-Marti,A., Fischer,T.,
            Gradillas,A., Sant'Anna,V.A.R., Were,F., Huang,Z.,
            Hernansanz-Agustin,P., Contreras,C., Martinez,F., Camafeita,E.,
            Vazquez,J., Ruiz-Cabello,J., Area-Gomez,E., Sanchez-Cabo,F.,
            Treuter,E., Bolanos,J.P., Estebanez-Perpina,E., Ruperez,F.J.,
            Barbas,C., Enriquez,J.A. and Ricote,M.
  TITLE     gamma-Linolenic acid in maternal milk drives cardiac metabolic
            maturation
  JOURNAL   Nature 618 (7964), 365-373 (2023)
   PUBMED   37225978
  REMARK    Erratum:[Nature. 2023 Jul;619(7968):E24. doi:
            10.1038/s41586-023-06316-w. PMID: 37322272]
REFERENCE   4  (bases 1 to 4148)
  AUTHORS   Selen,E.S., Rodriguez,S., Cavagnini,K.S., Kim,H.B., Na,C.H. and
            Wolfgang,M.J.
  TITLE     Requirement of hepatic pyruvate carboxylase during fasting, high
            fat, and ketogenic diet
  JOURNAL   J Biol Chem 298 (12), 102648 (2022)
   PUBMED   36441025
  REMARK    GeneRIF: Requirement of hepatic pyruvate carboxylase during
            fasting, high fat, and ketogenic diet.
REFERENCE   5  (bases 1 to 4148)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   6  (bases 1 to 4148)
  AUTHORS   Jitrapakdee,S., Petchamphai,N., Sunyakumthorn,P., Wallace,J.C. and
            Boonsaeng,V.
  TITLE     Structural and promoter regions of the murine pyruvate carboxylase
            gene
  JOURNAL   Biochem Biophys Res Commun 287 (2), 411-417 (2001)
   PUBMED   11554743
  REMARK    GeneRIF: Genomic structure of the pyruvate carboxylase gene is
            determined. The transcripts are highly expressed in kidney and
            liver, moderately expressed in heart and testis, and expressed at a
            low level in spleen.
REFERENCE   7  (bases 1 to 4148)
  AUTHORS   She,P., Shiota,M., Shelton,K.D., Chalkley,R., Postic,C. and
            Magnuson,M.A.
  TITLE     Phosphoenolpyruvate carboxykinase is necessary for the integration
            of hepatic energy metabolism
  JOURNAL   Mol Cell Biol 20 (17), 6508-6517 (2000)
   PUBMED   10938127
REFERENCE   8  (bases 1 to 4148)
  AUTHORS   Matsui,M., Araki,Y., Karasawa,H., Matsubara,N., Taketo,M.M. and
            Seldin,M.F.
  TITLE     Mapping of five subtype genes for muscarinic acetylcholine receptor
            to mouse chromosomes
  JOURNAL   Genes Genet Syst 74 (1), 15-21 (1999)
   PUBMED   10549128
REFERENCE   9  (bases 1 to 4148)
  AUTHORS   Zhang,J., Xia,W.L., Brew,K. and Ahmad,F.
  TITLE     Adipose pyruvate carboxylase: amino acid sequence and domain
            structure deduced from cDNA sequencing
  JOURNAL   Proc Natl Acad Sci U S A 90 (5), 1766-1770 (1993)
   PUBMED   8446588
REFERENCE   10 (bases 1 to 4148)
  AUTHORS   Freytag,S.O. and Collier,K.J.
  TITLE     Molecular cloning of a cDNA for human pyruvate carboxylase.
            Structural relationship to other biotin-containing carboxylases and
            regulation of mRNA content in differentiating preadipocytes
  JOURNAL   J Biol Chem 259 (20), 12831-12837 (1984)
   PUBMED   6548474
  REMARK    GeneRIF: Focuses on cloning of human pyruvate carboxylase but also
            studies pyruvate carboxylase levels in mouse preadipocyte
            differentiation.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY293312.1, BY230152.1, BG087228.2, AK148328.1, AK077665.1 and
            AA619324.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148328.1, AK170871.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                BY293312.1         2-92
            92-297              BY230152.1         80-285
            298-732             BG087228.2         234-668
            733-2210            AK148328.1         680-2157
            2211-4072           AK077665.1         1016-2877
            4073-4148           AA619324.1         17-92               c
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 4.07 cM"
     gene            1..4148
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /note="pyruvate carboxylase"
                     /db_xref="GeneID:18563"
                     /db_xref="MGI:MGI:97520"
     exon            1..110
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            111..147
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            148..283
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            284..468
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            469..634
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            635..780
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            781..898
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            899..1050
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1169
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1332
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1333..1515
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1660
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1750
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..2129
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2370
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2371..2620
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2865
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            2866..3045
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3046..3294
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3295..3435
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     exon            3436..4148
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4046..4051
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /note="hexamer: AATAAA"
     polyA_site      4073
                     /gene="Pcx"
                     /gene_synonym="Pc; Pcb"
                     /note="major polyA site"
ORIGIN      
        1 ggagccaggg ctgcagcaag tttggttgcg cggagaccgc agctgttccc tgtcagtgga
       61 ggcaggggcc gtcagaggcg gcggccacgg ctagaggcgg cagggcggag ctaacatcta
      121 ccttctggag agctgacaac actaatgatg ctgaagttcc aaacagttcg agggggcctg
      181 aggctcctgg gtgtccgccg atcctcctcg gcccctgttg cctccccaaa tgtccggcgt
      241 ctggagtata agcctatcaa gaaagtaatg gtggccaaca gaggtgagat tgccatccga
      301 gtgtttcgtg cgtgcacaga gctgggtatc cgcacagtgg ctgtctactc ggagcaggac
      361 acagggcaga tgcacaggca gaaagctgat gaagcctacc ttattggccg tggcctggca
      421 cctgtgcagg cctacctgca cattccagac atcatcaagg tggccaagga aaatggtgta
      481 gatgcggtgc atcctggcta tgggttcctc tcagagcgag cagactttgc ccaggcctgc
      541 caagatgctg gagtccggtt cattggtcca agcccagagg tggtccgcaa gatgggagac
      601 aaggtggaag cccgggccat tgccatcgct gcaggcgttc cagtggtccc cggcacggac
      661 tcccccatca gctccctgca cgaggcgcat gagttctcca acacctacgg cttccctatt
      721 atcttcaagg ccgcctacgg aggtgggggc cgcggcatgc gggtcgtgca tagctatgag
      781 gagttggaag agaattacac ccgggcctac tccgaggcct tggcagcctt tgggaatgga
      841 gcgttgtttg tggagaagtt cattgagaag ccaaggcaca ttgaggtgca gatcctaggg
      901 gaccagtatg ggaacatcct gcacctgtac gagcgagact gctccatcca gcgtcggcac
      961 cagaaggtgg tagagatcgc ccctgctacc cacctggatc cccaacttcg ctcacgtctc
     1021 accagtgact ctgtcaaact tgccaagcag gtaggctatg agaacgccgg cactgtggag
     1081 ttcctggtgg acaagcacgg caagcactac ttcatcgagg tcaattcccg cctgcaggtg
     1141 gagcacacgg tcaccgagga gatcacagat gtggacctgg tccatgctca gatccacgtg
     1201 tccgaaggcc ggagcctgcc tgacctgggc ctgcggcagg agaacatccg catcaatggc
     1261 tgtgccattc agtgtcgggt caccaccgag gaccctgcac gcagcttcca gccagacacc
     1321 ggccgcattg aggttttccg gagtggtgag ggcatgggca tccgcctgga caacgcctct
     1381 gcattccagg gcgctgtcat atcgccccac tatgactctc tgctcgtcaa ggtcattgca
     1441 cacggcaaag accaccccac agctgccacc aagatgagca gagccctggc cgagttccgt
     1501 gtccgaggtg taaagaccaa catccccttc ctgcagaatg ttctcaacaa ccagcagttc
     1561 ctggcaggca cagtggacac ccagttcatc gatgagaacc ctgagctgtt ccagcttcgg
     1621 cctgcacaga accgggccca gaagttgcta cattacctcg gacatgtcat ggtgaatggc
     1681 cctaccactc caatccctgt caatgtgagc cccagtcctg tggatcctgc tgttcctgtg
     1741 gtgcccatag gcccacctcc agctggtttc agggacatcc ttctgcgaga agggccagag
     1801 ggctttgccc gagctgtgcg gaatcaccag gggctgctgt tgatggacac aaccttccgg
     1861 gatgcccacc agtcactact ggccactaga gtgcgcacac atgatctcaa aaagattgcg
     1921 ccctatgttg cccacaactt caacaagctc ttcagcatgg agaactgggg aggcgccacg
     1981 ttcgacgttg ccatgcgctt cctgtacgag tgcccctggc ggcggctcca ggagctccgg
     2041 gagcttatcc cgaacatccc gttccagatg ctactgaggg gggccaatgc tgtgggctac
     2101 accaactacc ctgacaacgt ggtcttcaag ttctgtgagg tggccaaaga gaatggtatg
     2161 gacgtcttcc gagtctttga ctccctcaac tacttgccaa acatgctgct gggcatggaa
     2221 gcagcaggca gtgctggggg tgtggtggag gctgccatct catacacggg ggacgtggct
     2281 gaccctagtc gcactaaata ctcactggag tactacatgg gcttagctga agaactggtg
     2341 cgagctggca ctcacatcct gtgcattaag gacatggcgg gcctgctgaa gcctgccgcc
     2401 tgcaccatgc tggtcagctc cctccgggac cgattccccg acctcccact gcacatccat
     2461 acccatgata catcaggggc aggtgtggca gccatgctgg cctgtgcaca agcaggggct
     2521 gatgttgtgg acgtggcagt agactccatg tctgggatga cctcacagcc aagcatgggg
     2581 gccctggtgg cctgtaccaa agggactcct ttggacacag aggtacccct ggagcgtgtg
     2641 tttgactaca gtgagtactg ggaaggggct cggggactgt acgcagcctt cgattgcacg
     2701 gctaccatga agtctggcaa ctccgacgtg tatgagaatg agattccagg gggccagtac
     2761 accaacctgc acttccaggc ccatagcatg gggcttggct ccaagttcaa ggaggtcaag
     2821 aaggcctatg tggaggctaa ccagatgctg ggggacctca tcaaggtgac accatcctcc
     2881 aagattgtgg gggacctggc ccagttcatg gtgcagaatg ggttgagccg ggcagaggca
     2941 gaagctcagg cagaagagct gtccttcccc cgctctgtgg tggagttcct gcagggctac
     3001 attggcattc cccatggggg tttccctgag ccctttcgct ctaaggtgct aaaggacctg
     3061 ccaagaatag aggggcggcc tggagcctcc ctccctcccc tgaacctgaa ggagctggag
     3121 aaggacctga ttgataggca tggggaggag gtgaccccag aggacgtcct ctctgcagcc
     3181 atgtaccctg atgtctttgc tcaattcaaa gacttcacgg ctaccttcgg ccccctggat
     3241 agccttaata ctcgtctctt tcttcaagga cccaaaattg cagaggagtt tgaggttgag
     3301 ctggaacggg gcaagaccct gcacatcaaa gccctggctg taagcgacct gaaccgtgct
     3361 ggccagaggc aggtgttctt tgaactcaat gggcagcttc gatccattct ggttaaagac
     3421 acccaggcca tgaaggagat gcacttccat cccaaggctt tgaaggatgt gaagggccaa
     3481 attggggccc cgatgcctgg gaaggtcata gacatcaagg tggcagcagg ggacaaggtg
     3541 gctaagggcc agcccctctg tgtgctcagc gccatgaaga tggagactgt ggtgacttcg
     3601 cccatggagg gcactatccg aaaggttcat gttaccaagg acatgactct ggaaggcgac
     3661 gacctcatcc tagagattga gtgatcttac tccagcctgg cagcctggcc gaccctaccc
     3721 caagcctctc aacagaagct gtgcagccag ggcaggccca ggccagccag tacctgagag
     3781 taggaaggcc aggcccttga ggtcctgtcc catgggacag cacacacact acctgcaatg
     3841 gcctgcccat tcccttcagc tatttgtcct tttcttactg gcaggtagtt gctcacatat
     3901 tcatctcttg ccaaataagg gcctgctcct cgtgggagac cacaggtgta cagtcggtgg
     3961 ccttgtacct gggggaggtg gcttagggct tctacctctg ggggtagagg gaagacctaa
     4021 ttcacaggtc ctgggaaatt tgctcaataa aagtggcctt cccttgccct ccacactagg
     4081 tcatgtacag tctaccgcac cctagtgaca gtgtgggtgt atttctctca aactcccatg
     4141 ggatctag
//
