LOCUS       NM_008796               2844 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus phosphatidylcholine transfer protein (Pctp),
            transcript variant 1, mRNA.
ACCESSION   NM_008796
VERSION     NM_008796.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Druzak,S.A., Tardelli,M., Mays,S.G., El Bejjani,M., Mo,X.,
            Maner-Smith,K.M., Bowen,T., Cato,M.L., Tillman,M.C., Sugiyama,A.,
            Xie,Y., Fu,H., Cohen,D.E. and Ortlund,E.A.
  TITLE     Ligand dependent interaction between PC-TP and PPARdelta mitigates
            diet-induced hepatic steatosis in male mice
  JOURNAL   Nat Commun 14 (1), 2748 (2023)
   PUBMED   37173315
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Ersoy,B.A., Maner-Smith,K.M., Li,Y., Alpertunga,I. and Cohen,D.E.
  TITLE     Thioesterase-mediated control of cellular calcium homeostasis
            enables hepatic ER stress
  JOURNAL   J Clin Invest 128 (1), 141-156 (2018)
   PUBMED   29202465
REFERENCE   3  (bases 1 to 2844)
  AUTHORS   Li,J., Deng,S.P., Wei,G. and Yu,P.
  TITLE     CITGeneDB: a comprehensive database of human and mouse genes
            enhancing or suppressing cold-induced thermogenesis validated by
            perturbation experiments in mice
  JOURNAL   Database (Oxford) 2018 (2018)
   PUBMED   29688375
REFERENCE   4  (bases 1 to 2844)
  AUTHORS   Nicholls,H.T., Hornick,J.L. and Cohen,D.E.
  TITLE     Phosphatidylcholine transfer protein/StarD2 promotes microvesicular
            steatosis and liver injury in murine experimental steatohepatitis
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 313 (1), G50-G61 (2017)
   PUBMED   28385694
  REMARK    GeneRIF: These observations suggest that PC-TP promotes liver
            injury by mediating the intermembrane transfer of
            phosphatidylcholines, thus stabilizing more pathogenic
            microvesicular lipid droplets.
REFERENCE   5  (bases 1 to 2844)
  AUTHORS   Krisko,T.I., LeClair,K.B. and Cohen,D.E.
  TITLE     Genetic ablation of phosphatidylcholine transfer protein/StarD2 in
            ob/ob mice improves glucose tolerance without increasing energy
            expenditure
  JOURNAL   Metabolism 68, 145-149 (2017)
   PUBMED   28183446
  REMARK    GeneRIF: Improved glucose tolerance in Pctp(-/-);ob/ob mice in the
            absence of increases in energy expenditure or core body temperature
            indicates a direct pathogenic role for Phosphatidylcholine transfer
            protein in diabetes in leptin deficient mice
REFERENCE   6  (bases 1 to 2844)
  AUTHORS   Wu,M.K., Hyogo,H., Yadav,S.K., Novikoff,P.M. and Cohen,D.E.
  TITLE     Impaired response of biliary lipid secretion to a lithogenic diet
            in phosphatidylcholine transfer protein-deficient mice
  JOURNAL   J Lipid Res 46 (3), 422-431 (2005)
   PUBMED   15576839
REFERENCE   7  (bases 1 to 2844)
  AUTHORS   Romanowski,M.J., Soccio,R.E., Breslow,J.L. and Burley,S.K.
  TITLE     Crystal structure of the Mus musculus cholesterol-regulated START
            protein 4 (StarD4) containing a StAR-related lipid transfer domain
  JOURNAL   Proc Natl Acad Sci U S A 99 (10), 6949-6954 (2002)
   PUBMED   12011453
REFERENCE   8  (bases 1 to 2844)
  AUTHORS   Lammert,F., Carey,M.C. and Paigen,B.
  TITLE     Chromosomal organization of candidate genes involved in cholesterol
            gallstone formation: a murine gallstone map
  JOURNAL   Gastroenterology 120 (1), 221-238 (2001)
   PUBMED   11208732
  REMARK    Review article
REFERENCE   9  (bases 1 to 2844)
  AUTHORS   van Helvoort,A., de Brouwer,A., Ottenhoff,R., Brouwers,J.F.,
            Wijnholds,J., Beijnen,J.H., Rijneveld,A., van der Poll,T., van der
            Valk,M.A., Majoor,D., Voorhout,W., Wirtz,K.W., Elferink,R.P. and
            Borst,P.
  TITLE     Mice without phosphatidylcholine transfer protein have no defects
            in the secretion of phosphatidylcholine into bile or into lung
            airspaces
  JOURNAL   Proc Natl Acad Sci U S A 96 (20), 11501-11506 (1999)
   PUBMED   10500206
REFERENCE   10 (bases 1 to 2844)
  AUTHORS   Geijtenbeek,T.B., Smith,A.J., Borst,P. and Wirtz,K.W.
  TITLE     cDNA cloning and tissue-specific expression of the
            phosphatidylcholine transfer protein gene
  JOURNAL   Biochem J 316 (Pt 1) (Pt 1), 49-55 (1996)
   PUBMED   8645232
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY144797.1, BC071234.1, BB614866.1 and AL645695.18.
            
            On Oct 20, 2015 this sequence version replaced NM_008796.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053478.1, SRR6116558.83680.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164140
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                BY144797.1         2-45
            45-563              BC071234.1         1-519
            564-631             BB614866.1         412-479
            632-952             BC071234.1         520-840
            953-953             AL645695.18        119487-119487       c
            954-1960            BC071234.1         842-1848
            1961-2844           AL645695.18        117596-118479       c
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 54.7 cM"
     gene            1..2844
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /note="phosphatidylcholine transfer protein"
                     /db_xref="GeneID:18559"
                     /db_xref="MGI:MGI:107375"
     exon            1..193
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /inference="alignment:Splign:2.1.0"
     CDS             53..697
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /note="isoform 1 is encoded by transcript variant 1; START
                     domain-containing protein 2; stAR-related lipid transfer
                     protein 2"
                     /codon_start=1
                     /product="phosphatidylcholine transfer protein isoform 1"
                     /protein_id="NP_032822.2"
                     /db_xref="CCDS:CCDS25238.1"
                     /db_xref="GeneID:18559"
                     /db_xref="MGI:MGI:107375"
                     /translation="MAGAACCFSDEQFREACAELQKPALTGADWQLLVEASGITIYRL
                     LDQPSGLYEYKVFGVLEGCSPALLADVYMDLDYRKQWDQYVKELYEKESDEQMVAYWE
                     VKYPFPLSNRDYVYTRQRRDLDVDGRKIYVVLAQSISAPQFPEKSGVIRVKQYKQSLA
                     IESDGKKGSRVFMYYFDNPGGQIPSWLINWAAKNGVPNFLKDMVKACQNYHKKT"
     exon            194..311
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /inference="alignment:Splign:2.1.0"
     exon            312..391
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /inference="alignment:Splign:2.1.0"
     exon            392..563
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /inference="alignment:Splign:2.1.0"
     exon            564..631
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /inference="alignment:Splign:2.1.0"
     exon            632..2844
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1942..1947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /note="hexamer: AATAAA"
     polyA_site      1961
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /note="major polyA site"
     regulatory      2702..2707
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
                     /note="hexamer: ATTAAA"
     polyA_site      2723
                     /gene="Pctp"
                     /gene_synonym="PC-TP; StarD2"
ORIGIN      
        1 aagggccagt gagtgacctg cgctccgggc gctgtatcct ggctgcggaa ggatggcggg
       61 ggccgcatgc tgcttctcgg acgagcagtt ccgggaggct tgtgctgaac tccagaaacc
      121 ggcgctgacc ggggccgatt ggcagctcct ggtagaggcc tcaggcataa ccatctaccg
      181 gctgctggac cagccaagtg gactttatga gtataaggtg ttcggtgtcc tggaaggctg
      241 ctcgccggct ctcctcgcag atgtttacat ggacttagac tacagaaaac aatgggacca
      301 gtatgtgaaa gaactctacg agaaggagag tgatgaacag atggtggcat actgggaagt
      361 gaagtaccct ttcccgctgt ccaacagaga ttacgtctac acccgccagc gccgagacct
      421 ggatgtggac gggaggaaga tctacgtggt cctggcccag agtatctcgg cacctcagtt
      481 tcccgagaag tctggggtca tccgagtgaa acagtacaaa cagagcctgg cgatcgagag
      541 tgacggcaag aaggggagca gagttttcat gtactacttt gataacccgg gtggccaaat
      601 tccgtcctgg ctcattaact gggcagccaa gaatggagtt ccaaactttt tgaaggacat
      661 ggtgaaagcg tgtcagaact accacaagaa aacctaagga ggggactggg actccgtcat
      721 ccatgaagga catctctggc tgtgctacac cacctgaggc tcgcccttcc attcatactt
      781 cccatcttca aagcgctact cattcccaag caaccttgcc tctggccagc tccatcggct
      841 ccattgctgc cttctttccc aatggtcata ctcagagcct gttaccttct tccactattg
      901 aatggggccc atgcttgttt atgtttcatt agggaaatcc ttagtggggg gaaaaggcgc
      961 cattctgttg tgccatttgg gggggaatgg gtgaaaggag gaaggacgac aagctataga
     1021 agcagccctt cttggaaagc aggctcactt caggactctc tcttgcaccc agaagtggtc
     1081 tttagcccac agttgacctt ccatgttcga atactggaga tagagtgatg tctgttctct
     1141 tgatgcctcg ctgattcgga taggctgggg tttggggtga caagcaagtg gggctgtgtt
     1201 tcccccttgg gcggcattag tagctactat gatatcagac tcctctgagg ggtgtcccag
     1261 cactaacgga gcatatgttg agatgaccaa ggcttcctaa ttgcccacat ctggattttt
     1321 cctttcccat ccactgagaa atagctgcct ctacctctcg agctgtgcat ttgactgcaa
     1381 gcctcaggct tcaaagatgg cttgcgcata gcccaatgat agtgtcttcc tatgtactaa
     1441 ggtgactttc cattctagac tgtttggaga tgttgaaggg acagtaatgg tttacaatgg
     1501 agtgaaatgg aagtagtgat ttgatgtgct ctttgccccc ccccaccccc acaaggtttc
     1561 tctgtgtagc cctggctgtc ctggaactca ttctatagac caggttagcc tcgaactcag
     1621 aaatccacct gcctctgcct ctcgagtgct gggattaaag gcgtgtacca ccactgcccg
     1681 gcacttcgcc tttttcttag gaattctacc tcttcaaaac aggaactcag aaccctgcgt
     1741 ttgatcctcg atgttagtag gaaaatacat tctgtagcca agacacagcc ttaatgatct
     1801 caggggaaaa ttgtaaccac tgtgtgcaaa ccttgattaa cgaacatgga cactcaggat
     1861 gcaaaggcag aagaaaggga ttgtttaagt gggattcctt aaagtgtacg ttcttttctg
     1921 ggaagaaagt tgtgttctgt gaataaacgc agtgacttag gcttctctgt gtgctgtctc
     1981 cctcttacct ttctttgtgg aagaaggaat tagttatgtt gcctcttctg tttgcaggga
     2041 cacctggccc agctcaagac tagaaagaag ggaagaaaga aaggaagagg gggaaggagg
     2101 aagggaggga cagacagaca gacagacaaa cagaaaattt gtggctggag agatggctca
     2161 gctgtttata acactgagtg ttctttcagg ggacactggt tcacgtttag attgccaaca
     2221 cacaagtggc aactcatctc cttctatgta atttcagttt cagggcatgt gacaccctct
     2281 tctggcttcc acagataact gcatgcaaat catgccctta tggacatgca ggcaaaacac
     2341 ccattaaatt taaatgcatc ttttttaaga agagagaatt tacagctcac ttacattcaa
     2401 ggtcacgtac aacttctgga aggaaccagt agagtgcttg gttacattta aaattcagct
     2461 ttgttcttca atttctctaa atttgatgat agggttggta ctaatagcag taaaaaggct
     2521 catgccaagc agggtagaaa ggaaggaaag aaggaagaga gggcaggagg gaggaaggga
     2581 agaaggaaga tttcttgtca atgaacacag cttctctcta attttaactt tccctgtggc
     2641 atacgcctat tgatcccggg aataagatta tacaagtaaa cgaatgcttc gtaaaattgt
     2701 cattaaaacg ccattagcaa taagtgtctg gtatttgatt ttcgaaatat gggaactcat
     2761 tgtcatatat cgtgagtgta agttaatccc tgagcagtct ccttgagatg taaacaaaga
     2821 tcaaagcttt tgaacaggat cgac
//
