LOCUS       NM_001190483            7193 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus proprotein convertase subtilisin/kexin type 5 (Pcsk5),
            transcript variant 1, mRNA.
ACCESSION   NM_001190483
VERSION     NM_001190483.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Al Rifai,O., Susan-Resiga,D., Essalmani,R., Creemers,J.W.M.,
            Seidah,N.G. and Ferron,M.
  TITLE     In Vivo Analysis of the Contribution of Proprotein Convertases to
            the Processing of FGF23
  JOURNAL   Front Endocrinol (Lausanne) 12, 690681 (2021)
   PUBMED   34149625
  REMARK    GeneRIF: In Vivo Analysis of the Contribution of Proprotein
            Convertases to the Processing of FGF23.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7193)
  AUTHORS   Ito,H., Nozaki,K., Sakimura,K., Abe,M., Yamawaki,S. and Aizawa,H.
  TITLE     Activation of proprotein convertase in the mouse habenula causes
            depressive-like behaviors through remodeling of extracellular
            matrix
  JOURNAL   Neuropsychopharmacology 46 (2), 442-454 (2021)
   PUBMED   32942293
  REMARK    GeneRIF: Activation of proprotein convertase in the mouse habenula
            causes depressive-like behaviors through remodeling of
            extracellular matrix.
REFERENCE   4  (bases 1 to 7193)
  AUTHORS   Hoac,B., Susan-Resiga,D., Essalmani,R., Marcinkiweicz,E.,
            Seidah,N.G. and McKee,M.D.
  TITLE     Osteopontin as a novel substrate for the proprotein convertase 5/6
            (PCSK5) in bone
  JOURNAL   Bone 107, 45-55 (2018)
   PUBMED   29126984
  REMARK    GeneRIF: this study demonstrates that Pcsk5 is expressed in
            bone-forming cells, and that OPN is a novel substrate for PC5/6
REFERENCE   5  (bases 1 to 7193)
  AUTHORS   Constam,D.B., Calfon,M. and Robertson,E.J.
  TITLE     SPC4, SPC6, and the novel protease SPC7 are coexpressed with bone
            morphogenetic proteins at distinct sites during embryogenesis
  JOURNAL   J Cell Biol 134 (1), 181-191 (1996)
   PUBMED   8698813
REFERENCE   6  (bases 1 to 7193)
  AUTHORS   Mbikay,M., Seidah,N.G., Chretien,M. and Simpson,E.M.
  TITLE     Chromosomal assignment of the genes for proprotein convertases PC4,
            PC5, and PACE 4 in mouse and human
  JOURNAL   Genomics 26 (1), 123-129 (1995)
   PUBMED   7782070
REFERENCE   7  (bases 1 to 7193)
  AUTHORS   Seidah,N.G., Chretien,M. and Day,R.
  TITLE     The family of subtilisin/kexin like pro-protein and pro-hormone
            convertases: divergent or shared functions
  JOURNAL   Biochimie 76 (3-4), 197-209 (1994)
   PUBMED   7819324
  REMARK    Review article
REFERENCE   8  (bases 1 to 7193)
  AUTHORS   Nakagawa,T., Murakami,K. and Nakayama,K.
  TITLE     Identification of an isoform with an extremely large Cys-rich
            region of PC6, a Kex2-like processing endoprotease
  JOURNAL   FEBS Lett 327 (2), 165-171 (1993)
   PUBMED   8335106
REFERENCE   9  (bases 1 to 7193)
  AUTHORS   Lusson,J., Vieau,D., Hamelin,J., Day,R., Chretien,M. and
            Seidah,N.G.
  TITLE     cDNA structure of the mouse and rat subtilisin/kexin-like PC5: a
            candidate proprotein convertase expressed in endocrine and
            nonendocrine cells
  JOURNAL   Proc Natl Acad Sci U S A 90 (14), 6691-6695 (1993)
   PUBMED   8341687
REFERENCE   10 (bases 1 to 7193)
  AUTHORS   Nakagawa,T., Hosaka,M., Torii,S., Watanabe,T., Murakami,K. and
            Nakayama,K.
  TITLE     Identification and functional expression of a new member of the
            mammalian Kex2-like processing endoprotease family: its striking
            structural similarity to PACE4
  JOURNAL   J Biochem 113 (2), 132-135 (1993)
   PUBMED   8468318
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC133509.2, AC125203.3,
            AC126940.4 and AC147369.3.
            
            On Jul 8, 2015 this sequence version replaced NM_001190483.1.
            
            Summary: This gene encodes a subtilisin-like proprotein convertase
            that mediates posttranslational endoproteolytic processing of
            various proprotein substrates traversing the secretory pathway. The
            encoded protein is an inactive zymogen that undergoes
            autoproteolytic processing in the endoplasmic reticulum and the
            Golgi network to generate an active enzyme. Mice lacking the
            encoded protein die at an early embryonic stage. Conditional
            inactivation this gene in the epiblast but not in the
            extraembryonic tissue bypasses embryonic lethality but results in
            death at birth. Alternative splicing results in multiple transcript
            variants encoding different isoforms. [provided by RefSeq, Jul
            2015].
            
            Transcript Variant: This variant (1, also known as PC6B) represents
            the longer transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-678               AC133509.2         73802-74479         c
            679-783             AC125203.3         18134-18238
            784-897             AC125203.3         59691-59804
            898-1041            AC125203.3         96966-97109
            1042-1118           AC125203.3         99876-99952
            1119-1207           AC125203.3         133310-133398
            1208-1380           AC125203.3         136274-136446
            1381-1593           AC125203.3         156967-157179
            1594-1694           AC125203.3         169274-169374
            1695-1798           AC126940.4         18624-18727
            1799-1916           AC126940.4         40600-40717
            1917-2105           AC126940.4         41650-41838
            2106-2242           AC126940.4         50232-50368
            2243-2386           AC126940.4         55099-55242
            2387-2489           AC126940.4         60470-60572
            2490-2683           AC126940.4         63761-63954
            2684-2768           AC126940.4         68025-68109
            2769-2866           AC126940.4         71013-71110
            2867-2996           AC126940.4         71539-71668
            2997-3112           AC126940.4         72481-72596
            3113-3215           AC126940.4         111064-111166
            3216-3352           AC126940.4         121106-121242
            3353-3559           AC126940.4         124796-125002
            3560-3628           AC126940.4         126142-126210
            3629-3671           AC147369.3         177875-177917       c
            3672-3808           AC147369.3         174813-174949       c
            3809-4009           AC147369.3         172955-173155       c
            4010-4090           AC147369.3         169019-169099       c
            4091-4168           AC147369.3         166107-166184       c
            4169-4364           AC147369.3         163342-163537       c
            4365-4582           AC147369.3         161652-161869       c
            4583-4819           AC147369.3         156665-156901       c
            4820-5047           AC147369.3         154684-154911       c
            5048-5225           AC147369.3         151869-152046       c
            5226-5443           AC147369.3         147476-147693       c
            5444-5544           AC147369.3         139020-139120       c
            5545-5731           AC147369.3         136454-136640       c
            5732-7193           AC147369.3         132315-133776       c
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 12.86 cM"
     gene            1..7193
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /note="proprotein convertase subtilisin/kexin type 5"
                     /db_xref="GeneID:18552"
                     /db_xref="MGI:MGI:97515"
     exon            1..678
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            679..783
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            784..897
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1041
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1118
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1207
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1380
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1593
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1694
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1798
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1916
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2105
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            2106..2242
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2386
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            2387..2489
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2683
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            2684..2768
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            2769..2866
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            2867..2996
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            2997..3112
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3113..3215
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3216..3352
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3353..3559
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3560..3628
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3629..3671
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3672..3808
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            3809..4009
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4010..4090
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4091..4168
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4169..4364
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4365..4582
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4583..4819
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            4820..5047
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5048..5225
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5226..5443
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5444..5544
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5545..5731
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     exon            5732..7193
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7167..7172
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /note="hexamer: AATAAA"
     polyA_site      7193
                     /gene="Pcsk5"
                     /gene_synonym="b2b1549Clo; b2b585Clo; PC5; PC6; SPC6"
                     /note="major polyA site"
ORIGIN      
        1 actcacagtc ccggcgcgct gctcggagcc ggagggagcg cagcgagagg gcgagcgagc
       61 gagcgagcgc ttggagccgg gttcagcaga gggggcgccc ggccgctgct tgcgctccgg
      121 ccggtcgttt ccacggagct ccagggccgg agaggagcaa gaggcaaacg aagagtccaa
      181 gagcaaagcc gggagagccc gcggcggagg ctccagtccc actccgccgc ctcccgtgca
      241 agccggggag cgatcgctca acgccccgcc ggcccagagc ccgtcacccc gtcccctctg
      301 ccccaggcat ggtctaagag cctgagaggg gctagctgtt ctgctctgct cccgggctct
      361 cagcctccta ccgaccgctc ccggctgcgc gccccgccgg cccaggccgg agaagttagt
      421 tgtgcgcgct gcttagcgcg cgagccagcg ggcgggcgaa ggcggcgaag cgtcgggacc
      481 atggactggg actgggggaa ccgctgcagc cgcccgggac ggcgggacct gctgtgcgtg
      541 ctggcactgc tcgccggctg tctgctcccg gtatgccgga cgcgcgtcta caccaaccac
      601 tgggcagtga agatcgccgg cggcttcgcg gaggcagatc gcatagccag caagtacgga
      661 ttcatcaacg taggacagat cggtgcactg aaggactact atcacttcta ccatagtagg
      721 accattaaaa ggtctgttct ctcgagcaga ggaacccaca gtttcatttc aatggaacca
      781 aaggtggagt ggatccaaca gcaagtggtg aaaaaaagaa ccaagaggga ttatgacctc
      841 agccatgccc agtcaaccta cttcaatgat cccaagtggc caagtatgtg gtacatgcac
      901 tgcagtgaca acacccatcc ttgccagtca gacatgaata tcgaaggagc ctggaagaga
      961 ggctacacgg ggaagaatat cgtggtgact atcctggatg acggcatcga gagaacccac
     1021 ccagatctga tgcaaaacta cgatgctctg gcaagttgcg atgtgaatgg gaatgacttg
     1081 gacccgatgc ctcgttatga tgcaagcaat gagaacaagc atgggacccg ctgtgccgga
     1141 gaagtggcag ccactgcaaa caactctcac tgcactgtcg ggatcgcttt caacgccaag
     1201 attggaggtg tgagaatgct ggatggtgat gtcactgaca tggtggaggc aaagtctgtc
     1261 agctacaacc cacagcatgt gcacatctac agtgccagct ggggaccaga tgacgatggc
     1321 aagactgtgg atgggccagc tcccctcacc cggcaagcct ttgagaacgg cgtgagaatg
     1381 gggcggagag gccttggatc tgtgtttgtg tgggcatctg gcaatggtgg acggagcaag
     1441 gatcactgtt cttgtgatgg ctataccaac agcatctata ccatctccat cagcagtacg
     1501 gccgaaagtg gaaagaaacc ttggtacttg gaagagtgtt catctacact ggctacaacc
     1561 tacagcagtg gagaatccta tgataagaaa ataatcacta ctgatctaag gcagcgatgc
     1621 acagacaatc acactggaac atcagcctca gcccccatgg ctgctggaat cattgccctg
     1681 gccctagaag ccaatccgtt tctgacctgg agagacgtgc agcatgttat tgtcaggact
     1741 tcccgtgcgg gacatttgaa cgctaatgac tggaaaacca atgctgctgg ttttaaggtg
     1801 agccatctct atggatttgg actgatggat gccgaagcca tggtgatgga agcagagaag
     1861 tggacaactg ttcctcagca gcacgtgtgt gtggaaagca cagaccgaca aatcaagacc
     1921 attcgaccaa acagtgcagt gcgctccatc tacaaagcct caggctgctc ggataatccc
     1981 aaccatcacg tcaattacct ggagcatgta gttgtgcgta ttaccatcac acacccacgg
     2041 aggggagacc tggccatcta tctgacatca ccctcaggaa ccagatccca gctcttggcc
     2101 aacaggctct ttgatcattc catggaaggg tttaagaact gggagttcat gactattcat
     2161 tgctggggag aacgggctgc tggggactgg gtcctcgaag tttatgatac gccatctcag
     2221 ctgaggaact tcaagactcc aggtaaattg aaagaatggt ccttagtcct ctatggcacg
     2281 tccgtacagc catactcccc aaccaacgag tttcccaaag tggaacgctt ccgctacagc
     2341 cgagtggaag accccacaga tgactacggt gctgaagatt atgcaggtcc ctgtgaccct
     2401 gaatgcagtg aggttggatg tgacgggcca ggaccagatc actgcagtga ctgcttacac
     2461 tactactaca agctgaaaaa taacaccaga atctgtgtct ccagctgccc tcctggccac
     2521 taccatgctg acaagaagag atgccggaag tgtgccccaa actgcgagtc ctgctttggc
     2581 agccatggta atcagtgcct ctcctgtaaa tatggctact tcctgaatga agaaactagc
     2641 agctgtgtta ctcagtgccc tgatggatca tacgaggata tcaagaaaaa tgtctgtggg
     2701 aaatgcagtg agaactgcaa ggcatgcatt ggatttcaca actgcacaga gtgcaagggc
     2761 gggttaagtc ttcagggatc ccgctgttcg gtcacctgcg aggatggaca gttcttcaat
     2821 ggtcacgact gccagccctg ccatcgcttc tgtgctactt gctctggggc cggagcagat
     2881 ggatgtatta actgcaccga ggggtatgtc atggaagagg gaaggtgtgt acaaagttgt
     2941 agtgtgagct actacttgga ccactcttca gagggtggct acaaatcctg caagagatgt
     3001 gataacagct gtttgacatg caatgggcca ggattcaaga actgttccag ctgccccagt
     3061 ggatatcttt tagacttagg aacgtgtcag atgggagcca tctgcaagga tggagaatac
     3121 attgatgacc aaggccactg ccaaacctgt gaagcctcat gtgccaagtg ctggggacca
     3181 actcaggaag actgtatcag ctgccccgta acaagggtct tggatgatgg tcgctgtgtt
     3241 atgaactgtc cttcctggaa gttcgaattt aagaagcaat gccatccctg ccactacact
     3301 tgtcaaggat gccaaggtag tggcccttcc aactgcacct cctgtagagc agacaagcat
     3361 ggtcaggagc gcttcctgta ccacggggaa tgtctggaga actgccctgt gggccattat
     3421 cctgccaagg gacacgcctg cctaccctgc ccagacaact gtgagctttg ctacaaccca
     3481 cacgtctgca gtcgatgcat gagtggctat gtcatcattc cccccaacca cacctgccag
     3541 aagctggagt gcagacaagg tgaattccag gattctgagt atgaagaatg catgccctgt
     3601 gaggaaggat gtctgggatg cactgaggat gatccaggag cctgtacctc gtgtgctaca
     3661 ggatattaca tgtttgagcg gcattgctat aaagcctgtc ctgagaagac cttcggtgtg
     3721 aaatgggaat gcagggcctg tggtactaac tgtggcagct gcgaccaaca tgagtgctac
     3781 tggtgtgagg agggcttctt tctctccggt ggcagctgtg tgcaggattg tggccctggc
     3841 ttccatggag accaagagtt gggagaatgc aagccctgcc accgagcctg tgagaattgc
     3901 actggctcgg gctacaacca atgcagcagc tgtcaagaag ggttgcagct atggcatggg
     3961 acgtgtctct ggtccacctg gcctcaggtg gaaggcaagg actggaatga agccgtgccc
     4021 actgaaaagc catctttggt gaggagtctg ctgcaggatc gacgaaagtg gaaagttcaa
     4081 atcaaaagag atgcaacgag ccagaatcaa ccttgtcact cttcttgtaa aacctgcaat
     4141 ggatctctct gcgcttcatg tcccacaggt atgtacctgt ggctgcaggc ctgtgttcct
     4201 tcctgtcccc aaggcacttg gccatcagtc accagtggca gctgtgaaaa gtgttccgag
     4261 gactgtgtct cctgctccgg tgccgacctt tgccaacagt gcctgagcca gccggacaac
     4321 actctgcttc tccatgaggg caggtgctac cacagttgcc cagagggctt ttatgcaaaa
     4381 gatggtgttt gtgaacactg tagttcccct tgcaaaacat gcgaaggaaa tgccaccagc
     4441 tgtaactctt gtgaaggaga cttcgtccta gaccatgggg tgtgctggaa aacttgccct
     4501 gaaaagcacg tggccgtgga aggagtctgc aagcactgtc cagagaggtg ccaggactgc
     4561 atccacgaga aaacttgcaa agagtgcatg cctgacttct ttctatacaa tgacatgtgc
     4621 catcgttcct gtcccaagag cttctaccct gacatgcgcc agtgtgtccc ctgccacaaa
     4681 aactgtctgg agtgcaatgg ccccaaggaa gatgactgta aggtctgtgc tgatacttct
     4741 aaggctctcc acaatggatt gtgcttggat gagtgccctg agggaaccta caaagaagaa
     4801 gagaatgatg aatgcagaga ttgccccgag tcctgcctga tctgctcatc agcttggacc
     4861 tgcctggcct gtcgggaagg cttcacagta gtgcatgatg tctgcacggc acccaaggag
     4921 tgtgcagccg tcgagtactg ggatgagggc tcccacaggt gccagccgtg tcacaagaaa
     4981 tgctcccgct gctcagggcc ttctgaggac cagtgctata cctgtcccag agagactttt
     5041 cttctcaata caacctgtgt gaaagagtgc ccagagggct accacactga caaggacagc
     5101 cagcagtgtg tgctctgcca cagctcttgc aggacctgcg aaggaccaca cagcatgcag
     5161 tgcctctcct gccgacctgg ctggtttcaa ctgggcaagg agtgcttgct gcaatgcagg
     5221 gatggatatt atggagaaag caccagcggt aggtgtgaga agtgtgacaa gagctgcaag
     5281 tcatgccgag gtccacggcc cacagactgc cagtcttgcg atacattttt ctttctccta
     5341 cgctccaagg gacagtgcca ccgcgcttgc cctgagcact actatgcaga ccaacacgcc
     5401 cagacctgtg agaggtgcca ccccacttgt gacaagtgca gcggaaagga ggcgtggagt
     5461 tgcttgtcct gtgtgtggag ctaccacctt ctgaaaggaa tctgcatccc agaatgtatc
     5521 gtaggggaat acagagaggg aaagggagaa aactttaact gtaaaaaatg ccacgagagc
     5581 tgcatggaat gcaagggtcc aggcagcaag aactgcaccg ggtgctcagc tggcctgctg
     5641 ctggacatgg acgacaaccg ctgcctccat tgctgcaatg cctcccactc ccgcagatcc
     5701 caggactgct gcgactgcca gagttccaca gatgagtgca tccttccagc cagggaggct
     5761 gagttttacg agcacaccaa gactgctctg ctagtgacct ctggcgccat gctgttgctg
     5821 ctgctggggg ctgctgcggt cgtgtggcgg aagtctcgaa gcagacctgt ggcaaagggg
     5881 cggtacgaaa agctggcaga acctaccgtg tcatactcct cctacaggag cagctatctt
     5941 gacgaggacc aggtgattga gtacagggac cgggactacg atgaggacga tgaggacgac
     6001 atcgtctata tgggccaaga tggcactgtc taccggaagt tcaagtatgg gctgctggat
     6061 gagacggaag atgatgagtt ggagtacgat gatgagagct actcttacca ataaacagag
     6121 ccccctccca tctcaaaccc accacccacc acccacctcc catcccttcc tggtcccttc
     6181 ctggctctca atcctgctgg gagctaccaa tgcattggct gaggtctgag tgtttgcttt
     6241 tgtccttacc ttgcatccaa cccgaatatg tagggatgct ggagtctgct gtgctgcttt
     6301 tcattagctg agtgcaatga acagatcctg caaggagaga attcgaaggc attttccccg
     6361 agggtatgtc aagggtatta aatgcaggaa tctaaaagaa ctaagatctg ttgaacactc
     6421 taatgtgatt tttaaagtgg atggagaaga ggacctgtaa gggttctgct ttcaaactgt
     6481 agttggagac tcactctcat ccccacaact gctaccttct agaattctga tatttgacta
     6541 aatagagggg agatggagta ctagagagtt gttggagctg gggtgggagg tctctccact
     6601 ttcaatcgtg gaaagaaaga aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaga
     6661 aagaaagaaa gaaagaaaga aagaaagaaa gaaagacggg agggagggag ggagggaggg
     6721 agggagggaa agaaagaagg gagggaggga gggagggagg gagggaggga aggaaagaga
     6781 gagagagaga aagaaagaga gaaagaataa tcaatctgtg aagaccaaga aaggggactt
     6841 gaagtttctt aaacagtact cctgaagagc aaccacctcc tttgactgct taggacccat
     6901 ctatactgaa aggattagca gcctcagagc tgacagtcca cacgctagct gggccccagc
     6961 tcctttgcag aaacccagaa ccatagccac ctaagagcag aggaaggtca gacggaggga
     7021 aggcagcacc agggccagaa ggaaggaaac tgtttccaag actttccccc ttgtgatttt
     7081 ctaagagcct ctttgatagg aagtaataaa agaaaaattg taatagtaac acaaaagaca
     7141 aaaatttcac tcacgctcaa caattaaata aagacttcag ctgctaaatc aga
//
