LOCUS       NM_008793               2514 bp    mRNA    linear   ROD 07-FEB-2024
DEFINITION  Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4),
            mRNA.
ACCESSION   NM_008793
VERSION     NM_008793.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Scavuzzo,M.A., Chmielowiec,J., Yang,D., Wamble,K., Chaboub,L.S.,
            Duraine,L., Tepe,B., Glasgow,S.M., Arenkiel,B.R., Brou,C.,
            Deneen,B. and Borowiak,M.
  TITLE     Pancreatic Cell Fate Determination Relies on Notch Ligand
            Trafficking by NFIA
  JOURNAL   Cell Rep 25 (13), 3811-3827 (2018)
   PUBMED   30590051
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. PMID: 29144450]
REFERENCE   3  (bases 1 to 2514)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2514)
  AUTHORS   Mishra,P., Qiu,Q., Gruslin,A., Hidaka,Y., Mbikay,M. and Basak,A.
  TITLE     In vitro regulatory effect of epididymal serpin CRES on protease
            activity of proprotein convertase PC4/PCSK4
  JOURNAL   Curr Mol Med 12 (8), 1050-1067 (2012)
   PUBMED   22827436
  REMARK    GeneRIF: CRES may be a regulator of PC4 in sperm-egg interaction
            and fertilization.
REFERENCE   5  (bases 1 to 2514)
  AUTHORS   Tardif,S., Guyonnet,B., Cormier,N. and Cornwall,G.A.
  TITLE     Alteration in the processing of the ACRBP/sp32 protein and sperm
            head/acrosome malformations in proprotein convertase 4 (PCSK4) null
            mice
  JOURNAL   Mol Hum Reprod 18 (6), 298-307 (2012)
   PUBMED   22357636
  REMARK    GeneRIF: Data suggest that the fertility defect in PCSK4 knockout
            mice is due, in part, to altered processing/proteolysis of ACRBP
            (proacrosin/acrosin binding protein sp32) as well as structural
            abnormalities in sperm heads and acrosomes.
REFERENCE   6  (bases 1 to 2514)
  AUTHORS   Mbikay,M., Seidah,N.G., Chretien,M. and Simpson,E.M.
  TITLE     Chromosomal assignment of the genes for proprotein convertases PC4,
            PC5, and PACE 4 in mouse and human
  JOURNAL   Genomics 26 (1), 123-129 (1995)
   PUBMED   7782070
REFERENCE   7  (bases 1 to 2514)
  AUTHORS   Mbikay,M., Raffin-Sanson,M.L., Tadros,H., Sirois,F., Seidah,N.G.
            and Chretien,M.
  TITLE     Structure of the gene for the testis-specific proprotein convertase
            4 and of its alternate messenger RNA isoforms
  JOURNAL   Genomics 20 (2), 231-237 (1994)
   PUBMED   8020970
REFERENCE   8  (bases 1 to 2514)
  AUTHORS   Seidah,N.G., Chretien,M. and Day,R.
  TITLE     The family of subtilisin/kexin like pro-protein and pro-hormone
            convertases: divergent or shared functions
  JOURNAL   Biochimie 76 (3-4), 197-209 (1994)
   PUBMED   7819324
  REMARK    Review article
REFERENCE   9  (bases 1 to 2514)
  AUTHORS   Seidah,N.G., Day,R., Hamelin,J., Gaspar,A., Collard,M.W. and
            Chretien,M.
  TITLE     Testicular expression of PC4 in the rat: molecular diversity of a
            novel germ cell-specific Kex2/subtilisin-like proprotein convertase
  JOURNAL   Mol Endocrinol 6 (10), 1559-1570 (1992)
   PUBMED   1448111
REFERENCE   10 (bases 1 to 2514)
  AUTHORS   Nakayama,K., Kim,W.S., Torii,S., Hosaka,M., Nakagawa,T.,
            Ikemizu,J., Baba,T. and Murakami,K.
  TITLE     Identification of the fourth member of the mammalian endoprotease
            family homologous to the yeast Kex2 protease. Its testis-specific
            expression
  JOURNAL   J Biol Chem 267 (9), 5897-5900 (1992)
   PUBMED   1372895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC152062.6.
            
            [WARNING] On Mar 20, 2024 this sequence was replaced by
            NM_008793.3.
            
            On Jun 15, 2010 this sequence version replaced NM_008793.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D01093.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-203               AC152062.6         107646-107848
            204-308             AC152062.6         108069-108173
            309-401             AC152062.6         110867-110959
            402-530             AC152062.6         111041-111169
            531-607             AC152062.6         111270-111346
            608-696             AC152062.6         111448-111536
            697-869             AC152062.6         111714-111886
            870-1082            AC152062.6         111975-112187
            1083-1183           AC152062.6         113165-113265
            1184-1287           AC152062.6         113343-113446
            1288-1405           AC152062.6         113519-113636
            1406-1591           AC152062.6         113944-114129
            1592-1716           AC152062.6         114271-114395
            1717-1845           AC152062.6         114910-115038
            1846-2495           AC152062.6         115168-115817
            2496-2514           AC152062.6         115818-115836
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..2514
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /note="proprotein convertase subtilisin/kexin type 4"
                     /db_xref="GeneID:18551"
                     /db_xref="MGI:MGI:97514"
     exon            1..203
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     CDS             24..1991
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /note="prohormone convertase 3; KEX2-like endoprotease 3;
                     neuroendocrine convertase 3"
                     /codon_start=1
                     /product="proprotein convertase subtilisin/kexin type 4
                     precursor"
                     /protein_id="NP_032819.1"
                     /db_xref="CCDS:CCDS24018.1"
                     /db_xref="GeneID:18551"
                     /db_xref="MGI:MGI:97514"
                     /translation="MRPSQTELWLGLTLTLALLAVRWASAQAPIYVSSWAVRVTKGYQ
                     EAERLARKFGFVNLGQIFPDDQYFHLRHRGVAQQSLTPHWGHRLRLKKDPKVRWFEQQ
                     TLRRRVKRSLVVPTDPWFSKQWYMNKEIQQDLNILKAWNQGLTGRGVVISILDDGIEK
                     DHPDLWANYDPLASYDFNDYDPDPQPRYTPNDENRHGTRCAGEVSATANNGFCGAGVA
                     FNARIGGVRMLDGAITDIVEAQSLSLQPQHIHIYSASWGPEDDGRTVDGPGLLTQEAF
                     RRGVTKGRQGLGTLFIWASGNGGLHYDNCNCDGYTNSIHTLSVGSTTRQGRVPWYSEA
                     CASTFTTTFSSGVVTDPQIVTTDLHHQCTDKHTGTSASAPLAAGMIALALEANPLLTW
                     RDLQHLVVRASRPAQLQAEDWRINGVGRQVSHHYGYGLLDAGLLVDLARVWLPTKPQK
                     KCAIRVVHTPTPILPRMLVPKNVTACSDGSRRRLIRSLEHVQVQLSLSYSRRGDLEIF
                     LTSPMGTRSTLVAIRPLDISGQGYNNWIFMSTHYWDEDPQGLWTLGLENKGYYFNTGT
                     LYYYTLLLYGTAEDMTARPQAPQVTSRARACVQRDTEGLCQESHSPLSILAGLCLISS
                     QQWWWLYSHPQQPVTEGQASCHPPVTPAAAA"
     exon            204..308
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            309..401
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            402..530
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            531..607
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            608..696
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            697..869
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            870..1082
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1183
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1287
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1405
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1591
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1716
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1845
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..2514
                     /gene="Pcsk4"
                     /gene_synonym="NEC 3; NEC3; PC4; SPC5"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgcccccgc cccgcccccc gctatgcggc cctcccagac agagctgtgg ctgggtctga
       61 ctttgacttt ggccctcctg gctgtgaggt gggcctcagc ccaggccccc atctatgtca
      121 gcagctgggc agtgcgggtg accaaaggtt accaggaagc tgagcgcctg gcccgtaaat
      181 ttggcttcgt caacctggga cagatcttcc ctgacgacca gtatttccat ctgcggcacc
      241 ggggtgtggc ccagcagtcc ctgactccac actggggcca tcgtctacga ctgaagaaag
      301 atcccaaggt gcgctggttt gagcagcaga ctttgaggcg ccgggtgaaa cgctccctgg
      361 tggtgcccac agacccctgg ttttccaagc agtggtacat gaacaaggag atacaacaag
      421 atctcaacat cctaaaggct tggaaccagg gactgaccgg ccggggagtg gtgatttcca
      481 tcttggacga tggcattgag aaagaccacc cggacctctg ggctaattat gaccctctgg
      541 ccagctatga cttcaacgac tatgacccag acccccagcc tcgctacaca cccaacgatg
      601 agaaccgaca tggaacgcgc tgtgctgggg aggtgtctgc cacagctaac aacggtttct
      661 gtggtgccgg tgtggccttc aatgctagaa ttggaggcgt gcgcatgttg gatggagcca
      721 tcactgacat cgtggaggct cagtccctca gcctgcagcc gcaacacata cacatctata
      781 gcgccagctg gggcccggag gatgatggtc gcacggtgga cggacctggc ctcctcacac
      841 aggaggcctt caggcgtggt gtaaccaagg gccgccaagg gctgggtaca ctgttcatct
      901 gggcctcggg aaacggtggc ctccattacg acaactgcaa ttgtgacggc tacaccaaca
      961 gcatccacac actgtcagtg ggcagcacca cgcggcaggg tcgagtgccc tggtacagcg
     1021 aggcctgtgc ctccacgttt accaccacct tcagcagcgg tgtggtcacc gacccacaga
     1081 tcgtcaccac ggacctgcac caccagtgca ccgacaagca cacgggcacc tcggcatctg
     1141 ccccgctggc tgctggcatg atcgccctgg ccctggaggc caacccgctc ctgacctgga
     1201 gggacctaca gcacctggtg gtccgcgcgt ccaggccggc gcagctgcag gcggaggact
     1261 ggaggatcaa cggcgtgggg cgccaagtga gccaccatta tggctatggg ctgctggacg
     1321 cggggttgct ggtggacctg gctcgcgtgt ggctgcccac taagcctcag aagaaatgtg
     1381 ccattcgggt ggtgcacacc cccaccccca tcctgcctcg gatgctggtg ccgaagaacg
     1441 tgaccgcgtg ctcagatggc tcgcgccgcc gcctcatccg ctcgctcgag cacgtgcagg
     1501 tccagctttc gctctcctac agccgccgcg gggacctgga gatcttcctc accagcccca
     1561 tgggcacgcg ctccacgctc gtggccatca gaccgttgga tatcagcggc caaggctaca
     1621 acaactggat cttcatgtcc actcactact gggatgagga cccgcagggc ctgtggaccc
     1681 tgggcctgga gaataagggc tactatttta acacaggaac tctgtactac tacaccctgc
     1741 tgctgtatgg gacggccgag gacatgacag cacggcccca ggccccccag gtgaccagcc
     1801 gcgcgcgcgc atgtgtgcag agggacacag aggggctgtg ccaggaaagt cacagtcccc
     1861 tctccatcct ggcaggactc tgcctcatct ccagccagca gtggtggtgg ctctacagcc
     1921 acccacagca gccagtgacc gaaggacagg ccagctgtca ccctcctgtt acacctgctg
     1981 cagcagcctg agcagcgact acactgcctg tcccctgtcc cttgtgttgg gagagctcca
     2041 gagcccctcc aaggcttgcc acctctggtg gccccccagc taccagtctt gggccatggt
     2101 gctggctctg ctggccaggg cctttgggag gcccctcggc ttgaggaagg cccacctgcc
     2161 ccaggctaga taccccagtg cccaaaacca tgcatgcaca gatccctgga ggctggagag
     2221 ccagagatgc ctggctgtca ggacagaagg ccggtacccc aaggtcctgc tctcaggctg
     2281 gaagagaagc ctgccccaga aggccagggc agagcagctg catgggtcag gtgacagccc
     2341 accgctcagc ctcagctgct cccagtggaa gagatgtgtc cttactcatt ttggagcagg
     2401 tacagtgggc aagaggtcag accacagcca ccaatcatct gccccttccc tgtctccaag
     2461 ccatccatag ccccatgtct aacctcatag ttgccaaata aaagttaaac agaa
//
