LOCUS       NM_013892               2208 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus proprotein convertase subtilisin/kexin type 1
            inhibitor (Pcsk1n), mRNA.
ACCESSION   NM_013892
VERSION     NM_013892.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Amato,E. Jr., Taroc,E.Z.M. and Forni,P.E.
  TITLE     Illuminating the terminal nerve: Uncovering the link between GnRH-1
            neuron and olfactory development
  JOURNAL   J Comp Neurol 532 (3), e25599 (2024)
   PUBMED   38488687
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2208)
  AUTHORS   Aryal,D.K., Rodriguiz,R.M., Nguyen,N.L., Pease,M.W., Morgan,D.J.,
            Pintar,J., Fricker,L.D. and Wetsel,W.C.
  TITLE     Mice lacking proSAAS display alterations in emotion, consummatory
            behavior and circadian entrainment
  JOURNAL   Genes Brain Behav 21 (7), e12827 (2022)
   PUBMED   35878875
  REMARK    GeneRIF: Mice lacking proSAAS display alterations in emotion,
            consummatory behavior and circadian entrainment.
REFERENCE   4  (bases 1 to 2208)
  AUTHORS   Lee,D.R., Rhodes,C., Mitra,A., Zhang,Y., Maric,D., Dale,R.K. and
            Petros,T.J.
  TITLE     Transcriptional heterogeneity of ventricular zone cells in the
            ganglionic eminences of the mouse forebrain
  JOURNAL   Elife 11, e71864 (2022)
   PUBMED   35175194
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2208)
  AUTHORS   Shakya,M., Yildirim,T. and Lindberg,I.
  TITLE     Increased expression and retention of the secretory chaperone
            proSAAS following cell stress
  JOURNAL   Cell Stress Chaperones 25 (6), 929-941 (2020)
   PUBMED   32607937
  REMARK    GeneRIF: Increased expression and retention of the secretory
            chaperone proSAAS following cell stress.
            Erratum:[Cell Stress Chaperones. 2020 Nov;25(6):943. doi:
            10.1007/s12192-020-01138-5.. PMID: 32691307]
REFERENCE   6  (bases 1 to 2208)
  AUTHORS   Mzhavia,N., Berman,Y., Che,F.Y., Fricker,L.D. and Devi,L.A.
  TITLE     ProSAAS processing in mouse brain and pituitary
  JOURNAL   J Biol Chem 276 (9), 6207-6213 (2001)
   PUBMED   11094058
REFERENCE   7  (bases 1 to 2208)
  AUTHORS   Sayah,M., Fortenberry,Y., Cameron,A. and Lindberg,I.
  TITLE     Tissue distribution and processing of proSAAS by proprotein
            convertases
  JOURNAL   J Neurochem 76 (6), 1833-1841 (2001)
   PUBMED   11259501
REFERENCE   8  (bases 1 to 2208)
  AUTHORS   Fricker,L.D., McKinzie,A.A., Sun,J., Curran,E., Qian,Y., Yan,L.,
            Patterson,S.D., Courchesne,P.L., Richards,B., Levin,N., Mzhavia,N.,
            Devi,L.A. and Douglass,J.
  TITLE     Identification and characterization of proSAAS, a granin-like
            neuroendocrine peptide precursor that inhibits prohormone
            processing
  JOURNAL   J Neurosci 20 (2), 639-648 (2000)
   PUBMED   10632593
REFERENCE   9  (bases 1 to 2208)
  AUTHORS   Apletalina,E., Appel,J., Lamango,N.S., Houghten,R.A. and
            Lindberg,I.
  TITLE     Identification of inhibitors of prohormone convertases 1 and 2
            using a peptide combinatorial library
  JOURNAL   J Biol Chem 273 (41), 26589-26595 (1998)
   PUBMED   9756897
REFERENCE   10 (bases 1 to 2208)
  AUTHORS   Donadel,G., Marinos,N., DeSilva,M.G., Lu,J., Notkins,A.L. and
            Lan,M.S.
  TITLE     Molecular cloning and characterization of a highly basic protein,
            IA-4, expressed in pancreatic islets and brain
  JOURNAL   Neuroendocrinology 67 (3), 190-196 (1998)
   PUBMED   9630436
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL670169.9, AK076137.1, AK002969.1 and BE986911.1.
            
            On Sep 3, 2009 this sequence version replaced NM_013892.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF181560.1, AK076137.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                AL670169.9         21897-21932
            37-245              AK076137.1         3-211
            246-246             AL670169.9         24145-24145
            247-575             AK076137.1         212-540
            576-853             AK002969.1         502-779
            854-1607            AK076137.1         819-1572
            1608-2208           BE986911.1         1-601               c
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 3.57 cM"
     gene            1..2208
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /note="proprotein convertase subtilisin/kexin type 1
                     inhibitor"
                     /db_xref="GeneID:30052"
                     /db_xref="MGI:MGI:1353431"
     exon            1..223
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..886
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /note="granin-like neuroendocrine peptide; big SAAS;
                     little SAAS; big PEN LEN; little LEN; IA-4; pro-SAAS;
                     proprotein convertase 1 inhibitor; protein serine alanine
                     alanine serine; pancreas protein 3; basic protein IA-4"
                     /codon_start=1
                     /product="proSAAS precursor"
                     /protein_id="NP_038920.2"
                     /db_xref="CCDS:CCDS29979.1"
                     /db_xref="GeneID:30052"
                     /db_xref="MGI:MGI:1353431"
                     /translation="MAGSPLLCGPRAGGVGILVLLLLGLLRLPPTLSARPVKEPRSLS
                     AASAPLVETSTPLRLRRAVPRGEAAGAVQELARALAHLLEAERQERARAEAQEAEDQQ
                     ARVLAQLLRAWGSPRASDPPLAPDDDPDAPAAQLARALLRARLDPAALAAQLVPAPAA
                     APRPRPPVYDDGPTGPDVEDAGDETPDVDPELLRYLLGRILTGSSEPEAAPAPRRLRR
                     SVDQDLGPEVPPENVLGALLRVKRLENPSPQAPARRLLPP"
     exon            224..690
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /inference="alignment:Splign:2.1.0"
     exon            691..2193
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      999..1004
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /note="hexamer: AATAAA"
     polyA_site      1018
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /note="major polyA site"
     regulatory      1136..1141
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /note="hexamer: AATACA"
     polyA_site      1158
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
     regulatory      1587..1592
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /note="hexamer: ATTAAA"
     polyA_site      1608
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
     regulatory      2172..2177
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
                     /note="hexamer: AATAAA"
     polyA_site      2193
                     /gene="Pcsk1n"
                     /gene_synonym="bLEN; KEP; lLEN; Pan3; PEN; PEN19; PEN20;
                     proSAAS; SAAS; SAASCT"
ORIGIN      
        1 caggctcctg gatgtgcgca gccgcagagc gctgctgctg tgcctaatac ccatcgctgc
       61 gcacttgaca gccagtccgc ccgtccggag cccggctcgt tggggcagca tggcggggtc
      121 gccgctgctc tgcgggccgc gggccggggg cgtcggcatt ttggtgctgc tgctcttggg
      181 ccttctgagg ctgcccccca ccctgtcagc gaggcccgtg aaggagcccc gcagtctgag
      241 cgcagcatcc gcgcccttgg ttgagacgag cactcccctc cgcttgcgtc gggccgtgcc
      301 ccgaggagag gcggcgggtg cggtgcagga gctggcgcgg gcgctggcgc acctgctgga
      361 ggccgagaga caggaacgcg cgcgtgctga ggcgcaggag gctgaggatc agcaggcgcg
      421 tgtcctggcg cagctgctgc gcgcctgggg ctctccgcgt gcctcggacc cgcccttggc
      481 ccccgacgat gacccggacg ctccagctgc acagctcgcc cgtgctctgc tccgagctcg
      541 cctagacccg gccgccctgg cagcccaact tgtccccgcc cctgccgctg cgccgcgacc
      601 ccggccccca gtgtatgatg atggccccac tggcccagac gtcgaggatg ccggcgacga
      661 gactcctgac gtggaccctg agctgctgag gtacttgcta gggcggatcc tcaccggaag
      721 ttcggagcca gaggctgctc ctgccccgcg ccgcctccgc cgatctgtgg accaggattt
      781 gggtcccgag gtgccccctg agaacgtact gggggctctg ctacgcgtca aacgcctgga
      841 gaacccctcg ccccaggcgc cggcacgccg cctcctgcct ccctgagcgc tgctgcatcc
      901 tgcacgccct ggaacccagg agcgccccag caaccctgac tccctgccag cacgtccaag
      961 gctgcttacc ccagcaacct cccatcccct gagccctcaa taaatgccat ctgtagcagc
     1021 tgtttgtctg agcgccctgt actagggggc cggtgggctg ggtgacaatg ataatggaat
     1081 agtggctgtc ctactgagga cagcacagta ctgtttggga cctgtactgg taaggaatac
     1141 atgcctgctt cctctggact ttgcgggtct caccgggtgc ctgggctacc cttctaggct
     1201 tcactgaggc gggttccctg ggaggctctg aggttacttt cagcgtctgc caggggtcca
     1261 cagcacttag ccaaggggct atggattcac tcgtggtctg ccaggaccag gcttgttgtg
     1321 agggccccag gtggatcaca gacatccagc actgtatcag caactgccaa gcactgctta
     1381 cacggggctc tgaatattca ctccaaccat tgcctttgcc ctggccactt gttctattct
     1441 cagtatgtgc aagtttttgc acttcatagt cctttcttac ccttcctgtc actgtcttgt
     1501 gttggacaag gaaggaggtt gttttgtttg ttttgaggtg tatccaaagc acggttgggc
     1561 agtttctcaa ggcttttttt tttcctatta aaaaaaaatt gatacccagt cttactatgt
     1621 aatcctggtt gtcctgaaac tcactcggta caccaggcta gcctcaaact cagagattca
     1681 tctgcctgct tccctgagag ctgggattaa aggtgactgc cacacttggc tccttctttt
     1741 taaaaatatt cttaactgct gttgggcccg gtggtatatg tctttttaat cccagcacac
     1801 tggaggcagt ggcaggtaaa tctctgtgcc ttaaaggcca gactaatcta caaagtgagt
     1861 tccaggagag ttacatggag aaactctatt taaaaaaaaa aatcctaact gctgagtttt
     1921 caactttgtc ttccttttgg aagcagtctc actattgtag ctctggctat cctggaagat
     1981 ctcttgaact tccagcgggg ctacatagta agaacctgtc tccagaaaag gcaagaagaa
     2041 aagaaaaaca aagatttagt ataaaggaag ctttgaatcc tgagaacatt tatttaattt
     2101 tgcatagagt ttcataaaac acagtcaatg agggatgtcc catgttacca cgtaacttct
     2161 caccaaatga caataaaaag atgtctgtgg taaaaaaaaa aaaaaaaa
//
