LOCUS       NM_013628               5009 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus proprotein convertase subtilisin/kexin type 1 (Pcsk1),
            transcript variant 1, mRNA.
ACCESSION   NM_013628
VERSION     NM_013628.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Moeller,J. and Meier,D.T.
  TITLE     Ablation of PC1/3 in POMC-Expressing Tissues but Not in Immune
            Cells Induces Sepsis Hypersensitivity
  JOURNAL   J Endocr Soc 8 (11), bvae171 (2024)
   PUBMED   39435302
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5009)
  AUTHORS   Lu,C., Zhang,J., Wang,B., Gao,Q., Ma,K., Pei,S., Li,J. and Cui,S.
  TITLE     Casein kinase 1alpha is required to maintain murine hypothalamic
            pro-opiomelanocortin expression
  JOURNAL   iScience 26 (5), 106670 (2023)
   PUBMED   37168577
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5009)
  AUTHORS   Ni,Z., Wang,Y., Shi,C., Zhang,X., Gong,H. and Dong,Y.
  TITLE     Islet MC4R Regulates PC1/3 to Improve Insulin Secretion in T2DM
            Mice via the cAMP and beta-arrestin-1 Pathways
  JOURNAL   Appl Biochem Biotechnol 194 (12), 6164-6178 (2022)
   PUBMED   35900711
  REMARK    GeneRIF: Islet MC4R Regulates PC1/3 to Improve Insulin Secretion in
            T2DM Mice via the cAMP and beta-arrestin-1 Pathways.
REFERENCE   5  (bases 1 to 5009)
  AUTHORS   Meier,D.T., Rachid,L., Wiedemann,S.J., Traub,S., Trimigliozzi,K.,
            Stawiski,M., Sauteur,L., Winter,D.V., Le Foll,C., Bregere,C.,
            Guzman,R., Odermatt,A., Boni-Schnetzler,M. and Donath,M.Y.
  TITLE     Prohormone convertase 1/3 deficiency causes obesity due to impaired
            proinsulin processing
  JOURNAL   Nat Commun 13 (1), 4761 (2022)
   PUBMED   35963866
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5009)
  AUTHORS   Steiner,D.F., Smeekens,S.P., Ohagi,S. and Chan,S.J.
  TITLE     The new enzymology of precursor processing endoproteases
  JOURNAL   J Biol Chem 267 (33), 23435-23438 (1992)
   PUBMED   1429684
  REMARK    Review article
REFERENCE   7  (bases 1 to 5009)
  AUTHORS   Copeland,N.G., Gilbert,D.J., Chretien,M., Seidah,N.G. and
            Jenkins,N.A.
  TITLE     Regional localization of three convertases, PC1 (Nec-1), PC2
            (Nec-2), and furin (Fur), on mouse chromosomes
  JOURNAL   Genomics 13 (4), 1356-1358 (1992)
   PUBMED   1354647
REFERENCE   8  (bases 1 to 5009)
  AUTHORS   Seidah,N.G., Mattei,M.G., Gaspar,L., Benjannet,S., Mbikay,M. and
            Chretien,M.
  TITLE     Chromosomal assignments of the genes for neuroendocrine convertase
            PC1 (NEC1) to human 5q15-21, neuroendocrine convertase PC2 (NEC2)
            to human 20p11.1-11.2, and furin (mouse 7[D1-E2] region)
  JOURNAL   Genomics 11 (1), 103-107 (1991)
   PUBMED   1765368
REFERENCE   9  (bases 1 to 5009)
  AUTHORS   Korner,J., Chun,J., Harter,D. and Axel,R.
  TITLE     Isolation and functional expression of a mammalian prohormone
            processing enzyme, murine prohormone convertase 1
  JOURNAL   Proc Natl Acad Sci U S A 88 (15), 6834-6838 (1991)
   PUBMED   1862107
REFERENCE   10 (bases 1 to 5009)
  AUTHORS   Nakayama,K., Hosaka,M., Hatsuzawa,K. and Murakami,K.
  TITLE     Cloning and functional expression of a novel endoprotease involved
            in prohormone processing at dibasic sites
  JOURNAL   J Biochem 109 (6), 803-806 (1991)
   PUBMED   1657897
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158543.2.
            
            On Apr 13, 2018 this sequence version replaced NM_013628.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M69196.1, X57088.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-385               AC158543.2         91378-91762
            386-490             AC158543.2         94604-94708
            491-601             AC158543.2         97811-97921
            602-748             AC158543.2         99422-99568
            749-825             AC158543.2         100798-100874
            826-914             AC158543.2         107472-107560
            915-1087            AC158543.2         112339-112511
            1088-1300           AC158543.2         114063-114275
            1301-1401           AC158543.2         116758-116858
            1402-1635           AC158543.2         127371-127604
            1636-1793           AC158543.2         128245-128402
            1794-1927           AC158543.2         129331-129464
            1928-2089           AC158543.2         131563-131724
            2090-5009           AC158543.2         133463-136382
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 40.63 cM"
     gene            1..5009
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /note="proprotein convertase subtilisin/kexin type 1"
                     /db_xref="GeneID:18548"
                     /db_xref="MGI:MGI:97511"
     exon            1..385
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     CDS             206..2467
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /EC_number="3.4.21.93"
                     /note="neuroendocrine convertase 1; furin homolog;
                     prohormone convertase 1; propeptide-processing protease;
                     prohormone convertase 1/3"
                     /codon_start=1
                     /product="neuroendocrine convertase 1 precursor"
                     /protein_id="NP_038656.1"
                     /db_xref="CCDS:CCDS26649.1"
                     /db_xref="GeneID:18548"
                     /db_xref="MGI:MGI:97511"
                     /translation="MEQRGWTLQCTAFAFFCVWCALNSVKAKRQFVNEWAAEIPGGQE
                     AASAIAEELGYDLLGQIGSLENHYLFKHKSHPRRSRRSALHITKRLSDDDRVTWAEQQ
                     YEKERSKRSVQKDSALDLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWEKGITGKG
                     VVITVLDDGLEWNHTDIYANYDPEASYDFNDNDHDPFPRYDLTNENKHGTRCAGEIAM
                     QANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKT
                     VEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSA
                     SQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIF
                     ALALEANPNLTWRDMQHLVVWTSEYDPLASNPGWKKNGAGLMVNSRFGFGLLNAKALV
                     DLADPRTWRNVPEKKECVVKDNNFEPRALKANGEVIVEIPTRACEGQENAIKSLEHVQ
                     FEATIEYSRRGDLHVTLTSAVGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPVGT
                     WTLKITDMSGRMQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMV
                     NVVEKRPTQKSLNGNLLVPKNSSSSNVEGRRDEQVQGTPSKAMLRLLQSAFSKNALSK
                     QSPKKSPSAKLSIPYESFYEALEKLNKPSKLEGSEDSLYSDYVDVFYNTKPYKHRDDR
                     LLQALMDILNEEN"
     exon            386..490
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            491..601
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            602..748
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            749..825
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            826..914
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1087
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1088..1300
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1401
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1635
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1793
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1927
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2089
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..5009
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2625..2630
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /note="hexamer: AATGAA"
     polyA_site      2646
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /note="major polyA site"
     regulatory      4985..4990
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /note="hexamer: AATAAA"
     regulatory      4990..4995
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
                     /note="hexamer: ATTATA"
     polyA_site      5004
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
     polyA_site      5009
                     /gene="Pcsk1"
                     /gene_synonym="Nec-1; Nec1; PC1; PC3; Phpp-1; SPC3"
ORIGIN      
        1 tcagtcggga gactgaagca ctttagtgag cgctcgctct cgccgcccag cctctcccag
       61 tgagcctcta gctctagtag agcaacccag agccaggaga ggttttaagc tgctgggtgg
      121 aaaggtcgag tctagctggt gtgtctctga tcttgcttct tttctcccag cccttcctac
      181 ttgtgtgaga acaaggtttt gagccatgga gcaaagaggt tggactctgc agtgtactgc
      241 tttcgccttc ttttgcgttt ggtgtgcact aaacagtgta aaagcaaaga ggcagtttgt
      301 taatgaatgg gcggcggaga tccccggagg gcaagaagct gcctctgcca tcgccgaaga
      361 actggggtat gaccttttgg gtcagattgg atcacttgaa aatcactatt tattcaaaca
      421 caaaagccat cctcggaggt cccgaagaag cgctcttcat atcactaaga ggttatctga
      481 tgatgatcgt gtgacgtggg ctgaacaaca gtatgaaaaa gagagaagta aacgttcagt
      541 tcaaaaagac tcagcattgg atctcttcaa tgatccaatg tggaatcagc agtggtactt
      601 gcaagatacc agaatgactg cagctctgcc caagctggac cttcatgtaa tacctgtttg
      661 ggaaaagggt attactggca aaggagttgt tattactgta ctggatgatg gcttggagtg
      721 gaatcacaca gacatttatg ccaattatga tccagaggct agctatgatt ttaacgataa
      781 tgatcatgat ccatttcccc gatatgatct cacaaatgaa aacaaacatg gaacaagatg
      841 tgcaggtgaa attgccatgc aagcaaataa tcacaagtgt ggggttggag ttgcatataa
      901 ttccaaagtt ggaggcataa gaatgctgga tggcattgta actgatgcca ttgaggctag
      961 ttcaattgga ttcaaccctg gccatgtgga tatttacagt gcaagctggg gccctaatga
     1021 tgatggaaaa actgtggagg ggcctggcag actagcccag aaggcatttg aatatggtgt
     1081 caaacagggg agacaaggga aaggctccat ctttgtctgg gcttcaggga atgggggtcg
     1141 tcagggagat aactgtgact gtgatggcta cacagacagc atttacacca tctctatcag
     1201 cagtgcctcc cagcaaggcc tgtcaccttg gtatgcagag aagtgttctt ccacattggc
     1261 tacctcctac agcagtggtg attacacaga ccagcgaata acaagcgctg acctgcacaa
     1321 tgactgcaca gagacccaca caggcacctc ggcttcagca cccctggctg ctggtatctt
     1381 tgctctggcc ttggaggcaa acccaaatct tacctggaga gatatgcagc atctggttgt
     1441 ctggacctct gagtacgacc cattggccag taacccaggt tggaaaaaga atggggcagg
     1501 cttgatggtg aacagccgat ttggatttgg cttgctaaat gccaaagctc tggtggattt
     1561 ggctgatcct aggacctgga gaaatgtgcc tgagaagaaa gaatgtgttg taaaagacaa
     1621 taactttgag cctagagccc tgaaagctaa tggagaagta attgttgaaa tcccaacaag
     1681 agcttgtgaa ggacaagaaa atgctatcaa gtctctggaa catgtgcaat ttgaagcaac
     1741 aattgaatat tctcgtagag gagaccttca tgtcacactc acttctgctg ttggaaccag
     1801 cactgtactg ttggctgaaa gggaaagaga tacatccccc aatggcttta agaattggga
     1861 cttcatgtct gttcatacat ggggagagaa tcctgtaggc acctggacat tgaaaattac
     1921 agacatgtct ggaagaatgc aaaatgaagg aaggattgtg aactggaagt tgattttgca
     1981 tgggacatct tctcaaccag agcacatgaa gcagcctcgt gtgtacacat cctacaatac
     2041 agtccagaat gacaggagag gagtggaaaa gatggtgaat gttgtggaga agcggcccac
     2101 acaaaagagc ctgaatggca atctcctggt acccaaaaac tccagcagca gcaatgtgga
     2161 gggtagaagg gatgagcagg tacaaggaac tccttcaaag gccatgctgc gactcctaca
     2221 aagtgctttt agcaagaatg cactttcaaa acaatcacca aagaagtctc caagtgcaaa
     2281 gctcagcatc ccttatgaaa gtttctatga agccttggaa aagctcaaca agccctccaa
     2341 gcttgaaggc tctgaagaca gtctgtacag tgactatgtt gatgtattct ataacacaaa
     2401 accttataag catagagatg acaggctgct gcaagctctc atggacatcc taaatgagga
     2461 gaattaaaat aagtgtgttg cctgagttgg gaatcttcat gcaccctcct tttccttaaa
     2521 tgttcctggg tctggagttg ttgtgtccct ggtttttatg cttataatgt ccttcattgt
     2581 aattttactt tttctttcaa actgtacttt ggagtgagtt tcaaaatgaa agccaacttc
     2641 atcattcgtc tcagtgttta agagtatgtc ttccctgtct atacaagtga agcaacttct
     2701 ttctaaagtc attcttggga cacagccctt tactacagta gaaaagctcc taacacctcc
     2761 gcatttcata tctcacaagt ataggaaaag agtaggagat ccatttttat ttagaaaagt
     2821 aatcaagaaa ggtattttgg ctgctacagt ttatcctgtt ccctattttt gcaatggggt
     2881 tctttgaact ttgataagaa ggaagcattt ataaagtatc gaatgagaaa gttcacaaat
     2941 agattcatag ctggaataaa atgtattatt taaattcaga aactgatctt aaaatttata
     3001 ttagctagaa tagtcataca gtgttcctct tgattggtgt accatgtaga ggtctctatg
     3061 ggtcaaaagg tggctgtgac ccatgctatg taagacctgg ccttctattt tccccttgtg
     3121 ccttgtatgt ttcctttttg aaccacaaac tcaaaactca aagagctaat tcatgccatt
     3181 ttctcagaac ttatgcttga tttttttttt tttgcaaatt cctagccata gttttgttta
     3241 aataacaagt tttgtttaaa taacaaggag ccactcaaaa tacagagaat caatgaaata
     3301 acagggaaaa tgctttagca gagaagcaac aagatgcctt ttgcaagctg tgttcctagg
     3361 acaggttctt caggctctct atatgagtga tcatttctgg acaccacagg gtcttctcta
     3421 gtctcagagg acagtattca aattcttacc aaaatgcttc catgtttcaa gtcagaaaaa
     3481 aggtgggtaa cacttagatg gtatttatac tttctgtaag ttatgcagaa tatttcagaa
     3541 gtgtgaagga ttgatgaaag tgtgagatct cacatgaaac ctgattctca ggcccatcgt
     3601 ccatcacagc aatagctagc tagaggtaac actttccaag ttaagattta aagtccagcc
     3661 ttccctaacc caactttttt ttttacaata tctactttat ttgttactat tcagcatcca
     3721 gtagtcatct atcacctttt aattacaatc tgtgagttgc aggaagtaag gattcatttt
     3781 aacattctca tacacatatt tcatgtgctt gtacagtgtc cagccctcat tagtgtctct
     3841 tctgtctctc cttgcttgtt ctcttgctct tcacaagtat tcccacttcc aattccatgt
     3901 cttttttttt atctaatcca gattccaaat ataagagaaa atgtgatgtt tgtctttcta
     3961 tgcatgtaac tttgcccact cacatactac gtgaatctgt atagggtata aatacactag
     4021 gaaaagtatt tcctgaaatt atttccaagc agtctctgcc actaataatt gagcttgctg
     4081 taagttagat aaattgttgc atttataagt aaaaagagtt cttcagatcc taaaaacacc
     4141 tatgaacact tgatcatctc acctgaatgt tcatttgtaa tattcattaa ctagcgaccc
     4201 attctcttaa tatgccttcc ttctctgaat aggccaacat actttctagt taactctatg
     4261 cctgatttct ccaactgagt ttctgtttgc aaaatttcta caaagtctac acccactcct
     4321 aacactgaac tagctcagaa agacagctta tttgcctttg tcttgagtat ggttagtagc
     4381 acaagttttc aattcctgtt gaatcattag catttgtaca taaccaagcc ctctccagag
     4441 ccttgccctt gataccctgt ctttgaaggt tttgcattgt gttttctata aggtttaaat
     4501 gaaatgttta cttttatagc acattacaac tgaagaggac ttaagagttg taacattaaa
     4561 tttcagctac ttacaaaaga cctaaagaac agtatcacta actgacaatt aatatgtgat
     4621 ttaaaaaaat gaaagagttt gaggtcaacc ccacactttc ctgttcctta tactaaatta
     4681 tggagatttt gttgagaact ttctaagagc cccagacaac tcatgggcta tagccaaagg
     4741 ttcatttgga taagctttaa tatttagaaa ataccaggct tgaaatgttt ccaacctgga
     4801 gtatatattg tctagacata aaaaatattt ttgtcagtct ttcttagtgc ctatgtggac
     4861 tttttgtgaa aatgtttaaa aaaggataat tatataatat ttcccttgga attttaagct
     4921 atattttctt tacaggtatt tataatgtac caatgctttt atcaaacaga attttaaaag
     4981 gcataataaa ttatattaaa gaaccaaaa
//
