LOCUS       NM_029620               1866 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus procollagen C-endopeptidase enhancer 2 (Pcolce2),
            mRNA.
ACCESSION   NM_029620
VERSION     NM_029620.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Vadon-Le Goff,S., Tessier,A., Napoli,M., Dieryckx,C., Bauer,J.,
            Dussoyer,M., Lagoutte,P., Peyronnel,C., Essayan,L., Kleiser,S.,
            Tueni,N., Bettler,E., Mariano,N., Errazuriz-Cerda,E., Fruchart
            Gaillard,C., Ruggiero,F., Becker-Pauly,C., Allain,J.M.,
            Bruckner-Tuderman,L., Nystrom,A. and Moali,C.
  TITLE     Identification of PCPE-2 as the endogenous specific inhibitor of
            human BMP-1/tolloid-like proteinases
  JOURNAL   Nat Commun 14 (1), 8020 (2023)
   PUBMED   38049428
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Xu,H., Thomas,M.J., Kaul,S., Kallinger,R., Ouweneel,A.B.,
            Maruko,E., Oussaada,S.M., Jongejan,A., Cense,H.A., Nieuwdorp,M.,
            Serlie,M.J., Goldberg,I.J., Civelek,M., Parks,B.W., Lusis,A.J.,
            Knaack,D., Schill,R.L., May,S.C., Reho,J.J., Grobe,J.L.,
            Gantner,B., Sahoo,D. and Sorci-Thomas,M.G.
  TITLE     Pcpe2, a Novel Extracellular Matrix Protein, Regulates Adipocyte
            SR-BI-Mediated High-Density Lipoprotein Uptake
  JOURNAL   Arterioscler Thromb Vasc Biol 41 (11), 2708-2725 (2021)
   PUBMED   34551590
  REMARK    GeneRIF: Pcpe2, a Novel Extracellular Matrix Protein, Regulates
            Adipocyte SR-BI-Mediated High-Density Lipoprotein Uptake.
REFERENCE   3  (bases 1 to 1866)
  AUTHORS   Pollard,R.D., Blesso,C.N., Zabalawi,M., Fulp,B., Gerelus,M.,
            Zhu,X., Lyons,E.W., Nuradin,N., Francone,O.L., Li,X.A., Sahoo,D.,
            Thomas,M.J. and Sorci-Thomas,M.G.
  TITLE     Procollagen C-endopeptidase Enhancer Protein 2 (PCPE2) Reduces
            Atherosclerosis in Mice by Enhancing Scavenger Receptor Class B1
            (SR-BI)-mediated High-density Lipoprotein (HDL)-Cholesteryl Ester
            Uptake
  JOURNAL   J Biol Chem 290 (25), 15496-15511 (2015)
   PUBMED   25947382
  REMARK    GeneRIF: PCPE2 was shown to enhance SR-BI function by increasing
            the rate of HDL-associated cholesteryl ester uptake, possibly by
            optimizing SR-BI localization and/or conformation.
REFERENCE   4  (bases 1 to 1866)
  AUTHORS   Baicu,C.F., Zhang,Y., Van Laer,A.O., Renaud,L., Zile,M.R. and
            Bradshaw,A.D.
  TITLE     Effects of the absence of procollagen C-endopeptidase enhancer-2 on
            myocardial collagen accumulation in chronic pressure overload
  JOURNAL   Am J Physiol Heart Circ Physiol 303 (2), H234-H240 (2012)
   PUBMED   22610170
  REMARK    GeneRIF: It was shown that PCOLCE2 is required for efficient
            procollagen processing and deposition of fibrillar collagen in the
            pressure-overloaded myocardium.
REFERENCE   5  (bases 1 to 1866)
  AUTHORS   Naba,A., Clauser,K.R., Hoersch,S., Liu,H., Carr,S.A. and Hynes,R.O.
  TITLE     The matrisome: in silico definition and in vivo characterization by
            proteomics of normal and tumor extracellular matrices
  JOURNAL   Mol Cell Proteomics 11 (4), M111.014647 (2012)
   PUBMED   22159717
REFERENCE   6  (bases 1 to 1866)
  AUTHORS   Heinzel,K. and Bleul,C.C.
  TITLE     The Foxn1-dependent transcripts PCOLCE2 and mPPP1R16B are not
            required for normal thymopoiesis
  JOURNAL   Eur J Immunol 37 (9), 2562-2571 (2007)
   PUBMED   17683113
REFERENCE   7  (bases 1 to 1866)
  AUTHORS   Barnett,K.R., Tomic,D., Gupta,R.K., Babus,J.K., Roby,K.F.,
            Terranova,P.F. and Flaws,J.A.
  TITLE     The aryl hydrocarbon receptor is required for normal gonadotropin
            responsiveness in the mouse ovary
  JOURNAL   Toxicol Appl Pharmacol 223 (1), 66-72 (2007)
   PUBMED   17594909
REFERENCE   8  (bases 1 to 1866)
  AUTHORS   Hecht,J., Seitz,V., Urban,M., Wagner,F., Robinson,P.N., Stiege,A.,
            Dieterich,C., Kornak,U., Wilkening,U., Brieske,N., Zwingman,C.,
            Kidess,A., Stricker,S. and Mundlos,S.
  TITLE     Detection of novel skeletogenesis target genes by comprehensive
            analysis of a Runx2(-/-) mouse model
  JOURNAL   Gene Expr Patterns 7 (1-2), 102-112 (2007)
   PUBMED   16829211
REFERENCE   9  (bases 1 to 1866)
  AUTHORS   Steiglitz,B.M., Keene,D.R. and Greenspan,D.S.
  TITLE     PCOLCE2 encodes a functional procollagen C-proteinase enhancer
            (PCPE2) that is a collagen-binding protein differing in
            distribution of expression and post-translational modification from
            the previously described PCPE1
  JOURNAL   J Biol Chem 277 (51), 49820-49830 (2002)
   PUBMED   12393877
  REMARK    GeneRIF: PCPE2 is shown to be a glycoprotein that differs markedly
            in the nature of its glycosylation from that of PCPE1
REFERENCE   10 (bases 1 to 1866)
  AUTHORS   Steiglitz,B.M. and Greenspan,D.S.
  TITLE     Assignment of the mouse Pcolce2 gene, which encodes procollagen
            C-proteinase enhancer protein 2, to chromosome 9 and localization
            of PCOLCE2 to human chromosome 3q23
  JOURNAL   Cytogenet Cell Genet 95 (3-4), 244-245 (2001)
   PUBMED   12063410
  REMARK    GeneRIF: Assignment of the mouse Pcolce2 gene, which encodes
            procollagen C-proteinase enhancer protein 2, to chromosome 9.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK010249.1 and AK132395.1.
            
            On Jan 12, 2006 this sequence version replaced NM_029620.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660811.47976.1, AK010249.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-111               AK010249.1         2-112
            112-1866            AK132395.1         2-1756
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 50.18 cM"
     gene            1..1866
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /note="procollagen C-endopeptidase enhancer 2"
                     /db_xref="GeneID:76477"
                     /db_xref="MGI:MGI:1923727"
     exon            1..246
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /inference="alignment:Splign:2.1.0"
     CDS             167..1411
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /note="PCPE-2; procollagen C-proteinase enhancer 2;
                     procollagen COOH-terminal proteinase enhancer 2"
                     /codon_start=1
                     /product="procollagen C-endopeptidase enhancer 2
                     precursor"
                     /protein_id="NP_083896.1"
                     /db_xref="CCDS:CCDS23410.1"
                     /db_xref="GeneID:76477"
                     /db_xref="MGI:MGI:1923727"
                     /translation="MGGASACIPLCLLLATARMARPQTPERPVFTCGGILTGESGFIG
                     SEGFPGMYPPNSKCTWKITVPEGKVVVLNFRFIDLENDNLCRYDFVDVYNGHANGQRI
                     GRFCGTFRPGSLVASGNKMTVQMISDANTAGSGFMATYSAAAPDGKGDRYCGGRLEKP
                     SGTFKTPNWPDRDYPVGVTCVWHIIAPKNQLIELKFEKFDVERDNYCRYDYVAVFNGG
                     EVNDAKRIGKYCGDSPPVPIVSERNELLIQFLSDLSLTADGFIGHYKFRPKKFPTTTT
                     TPVTTTLPVTTGLKPTVALCQQKCRRMGTLESNYCSSNFVLAGTVITTVTRGGSLHAT
                     VSIISIYREGNLAIQQAGKNMSVKLTVVCRQCPLLRRGLNYIIMGQVGEDGRGKIMPN
                     SFVKMFKNKNQKPMNALKNKQC"
     exon            247..355
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /inference="alignment:Splign:2.1.0"
     exon            356..611
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /inference="alignment:Splign:2.1.0"
     exon            612..736
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /inference="alignment:Splign:2.1.0"
     exon            737..873
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1028
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1112
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1280
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..1866
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1844..1849
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /note="hexamer: AATAAA"
     polyA_site      1863
                     /gene="Pcolce2"
                     /gene_synonym="2400001O18Rik; Pcpe2"
                     /note="major polyA site"
ORIGIN      
        1 aaacgggaca ttcagccgcc gcccgcggct gccagggcgc gtcccctggg gaaattaggc
       61 gtggagtccg cccggatcgc cttcaaaaac cgccctcctg ccggccgcgg cgcactctgt
      121 acctggctgc gggcgcccgg cgggatgctg cagtgagctg ccggccatgg ggggcgcgag
      181 cgcctgcatc ccgctctgcc tgcttctggc caccgcgcgg atggcccggc cgcagacgcc
      241 agagagacct gttttcacat gtggcggcat tcttaccgga gagtctggat ttattggcag
      301 tgaaggtttt cctggaatgt accctccgaa tagcaaatgt acttggaaaa tcacagttcc
      361 tgagggcaaa gtggtcgtct tgaattttcg attcatagac ctggagaatg acaacctgtg
      421 tcgctatgac ttcgtggatg tgtacaatgg ccacgccaat ggccagcgta tcgggcgctt
      481 ctgtggcaca ttccgacctg gatcgctcgt agccagcggc aacaagatga cggtacaaat
      541 gatttctgac gccaacaccg cgggaagtgg cttcatggca acgtactctg ccgctgcgcc
      601 agatggaaaa ggagaccggt attgtggagg acgccttgaa aaaccttccg gtacctttaa
      661 aactcccaac tggccagacc gagattaccc cgtgggagtt acttgtgtgt ggcacatcat
      721 agctccgaag aaccagctta tagagttaaa gtttgagaag ttcgatgttg agcgcgataa
      781 ctactgcaga tacgactacg tggctgtgtt taacggtggg gaagtcaatg acgccaaaag
      841 aattgggaag tactgtgggg acagtccacc tgtgccgatc gtgtctgaga gaaatgaact
      901 tcttattcag ttcttatcag acttaagttt aacagcggat ggttttattg gtcactacaa
      961 attcaggccg aaaaagtttc ccacaaccac caccacgccc gtcaccacca cactccccgt
     1021 aactacaggt ctaaagccca ctgtggccct gtgtcagcag aagtgtagac ggatggggac
     1081 tctggagagc aattactgtt caagcaactt cgtgttagcc ggcacagtta tcacgaccgt
     1141 cactcgaggt gggagcttgc acgccacagt ctcgatcatc agcatctaca gggaaggcaa
     1201 cctggccatt cagcaggctg gcaagaacat gagtgtcaag ctcactgtgg tgtgcaggca
     1261 gtgccccctt ctcagacgag gtctcaatta cattattatg ggacaagtgg gtgaagatgg
     1321 gcgtggcaaa atcatgccaa acagctttgt caaaatgttc aagaataaga atcagaagcc
     1381 catgaacgca ctgaagaaca agcagtgtta gcctgagctc tgtcacataa cctgaactta
     1441 ctcatcaccg ctgaacgatc tgtccacgtt aggaaaaaaa aaaaaaaaaa accacgctat
     1501 ttaatgctga gcgttctgag agatggagct gagtaactcc tcacgcgatg gatgtgtgag
     1561 ccacgccccc agttcactgg tggaaggtct gcgcctgcgc tgtgatctcc aacatctgcg
     1621 aactcaccca cccagtgcct gatggggaag agcagcgagc tccgacatct ggaagcttga
     1681 acatacatct ctgtaggagg atattctaga gtcgagttgt atgaagatat acaaaggatt
     1741 ttataaagtg caatatttat aatgatattt ggttaccttc aagcatttgc tctgaggtgt
     1801 cacattcttt ttttctcttt cattttttta aaagtcaatg cttaataaaa tattttaaat
     1861 gattat
//
