LOCUS       NM_026327               5968 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus pecanex homolog 4 (Pcnx4), transcript variant 1, mRNA.
ACCESSION   NM_026327
VERSION     NM_026327.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160936.2.
            
            On Apr 15, 2022 this sequence version replaced NM_026327.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029996.1, AK136802.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-163               AC160936.2         83579-83741         c
            164-906             AC160936.2         78001-78743         c
            907-1086            AC160936.2         64502-64681         c
            1087-1601           AC160936.2         63760-64274         c
            1602-1702           AC160936.2         63435-63535         c
            1703-1822           AC160936.2         63198-63317         c
            1823-2186           AC160936.2         52858-53221         c
            2187-2290           AC160936.2         50297-50400         c
            2291-3303           AC160936.2         45616-46628         c
            3304-3490           AC160936.2         44578-44764         c
            3491-5968           AC160936.2         38337-40814         c
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 30.2 cM"
     gene            1..5968
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /note="pecanex homolog 4"
                     /db_xref="GeneID:67708"
                     /db_xref="MGI:MGI:1914958"
     exon            1..163
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     exon            164..906
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     CDS             209..3742
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     pecanex-like protein C14orf135 homolog; pecanex-like
                     protein 4; pecanex-like 4; pecanex homolog protein 4"
                     /codon_start=1
                     /product="pecanex-like protein 4 isoform 1"
                     /protein_id="NP_080603.2"
                     /db_xref="CCDS:CCDS36473.1"
                     /db_xref="GeneID:67708"
                     /db_xref="MGI:MGI:1914958"
                     /translation="MGSMSSDVPLLNDYKQDFLLKRFPQTVLGGPRLKLGYCAPPYIY
                     VNQIVLFLMPWALGGTGTLLYQLDILRDYTAAALSGGLMVFTAAVIQLISVYARSKSV
                     VVRRMRTRDILAEEDQHEFTSCAGAETVKFLIPGKKYVANTVFHSVLAGLVCGLGTWY
                     LLPNRVTLLYGSPGATAVLFVFGWITLCIGEYSLIVNTATETATFQTQDTYEITPLMR
                     PLYIFFFVSVDLAHRFIVNIPALEQMNQILHILFVLLPFLWALGTLPPPDALLFWAVE
                     QVLEFGLGGSSMSTHLRLLVMFIVSAGAAVVSYFIPSTVGVVLFMTGLGFLLSLNPSD
                     VSMVFRYGVTRHRVGSSHEARPGNSGHWFPWKECFLFIAVLGMALLEAGLLHHYVSVP
                     QVSRSGAQAVVGYVLMVLLSIVWILRETQSIYIFGIFRNPFYPKDIHAVSVFYDKQNK
                     LLKIGAVRWILLTLVSPLAMVAFLALDSSLHGLHSVSVSIGFTRAFRMVWQNTETALL
                     ETVVVSAVHMVSSTDCWWNRSLDTGIRLLLIGIMRDRLIQFITKLQFAVTVLLALWTE
                     KKQHRKTTTTLCVLNTVFAPFVLGIIVLSTLLSSPLLPLFTLPVFLVGFPRPVQSWPG
                     AVGTAACVCTDSVFYHQMVPRLTAALQTAMAAGSLGLLLPGSHYLGRFEDRLIWIMIL
                     ECGYTYCCINVKGLELQETSCHTAEAQRVDEVFESAFQREHPQVCSLNEHLENVLTPC
                     TVLPVKLYSDARNVLTGIIDSPDNLKEFKDDLIKVLVWVLIQHCSKRPSTQENKTENT
                     GEASPALPPAANSSPCPESLEDSESANSDWSDGSIFDDEPAIKNRKEKLQSKDLPCTK
                     LPIPGSVDSQNPDDHSAGTGPKNDLYRTVILGLPAVDKGQREDVAYIPLVEFSCSQSR
                     LLSLPEEWRSNSTPRSKILEMSALFPEDWYQFVLRQLECFHSEEKSRVLEEIAKDKAL
                     KDLYVHTVMACYIGLFGIDNGVPSPGQLVRVYNGGLPWAGTLDWLSEKPELFHLVRKA
                     FRYTLKLMVDKASLGPIEDFKELTNCLREYERDWYIGLVSEEQWKRAILEEKPCLFCL
                     GYESSMGVYTSRVLMLQEMSVHIGKLNAEAVRGQWANLSWELLYATNDDEERYSIQAH
                     PLLLRNLTVQAADPPLGYPIFSSKPLPIHLC"
     exon            907..1086
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1601
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     exon            1602..1702
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1822
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..2186
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     exon            2187..2290
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     exon            2291..3303
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     exon            3304..3490
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     exon            3491..5968
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4286..4291
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /note="hexamer: AATAAA"
     polyA_site      4306
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /note="major polyA site"
     polyA_site      5157
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
     regulatory      5932..5937
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /note="hexamer: AATAAA"
     regulatory      5946..5951
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
                     /note="hexamer: AATAAA"
     polyA_site      5955
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
     polyA_site      5968
                     /gene="Pcnx4"
                     /gene_synonym="1810048J11Rik; 4932413O10; Pcnxl4"
ORIGIN      
        1 gcagttgatc ttagccatgg gaacgggagc ggtcctggag gcctccggtc agctgctggc
       61 ggggcccgca tccatcctcg ggcaggagtt cccaaccgac ttcagctggg gagatgtggt
      121 cccaggacgc cctcgcgtcg cctctcccct gcggtgactc cagatatttc tggttataaa
      181 aaagatgtga ctctcagact tatcctgaat gggaagtatg agttcagatg tccctctgct
      241 aaatgattac aaacaggact tcctgctgaa gcgctttcca cagactgttc ttggaggccc
      301 tcgactcaaa ctaggctact gtgcccctcc ttacatatat gttaatcaga ttgtcctttt
      361 cctgatgcca tgggccttag gtggaacggg aacgcttcta taccaactgg atatcctgag
      421 agactatact gcagccgcgc tttcaggagg actaatggtg ttcactgcgg ctgtcatcca
      481 gctcataagc gtgtacgcca ggagcaagtc ggtggtggtc aggaggatgc gaaccaggga
      541 tatcttagca gaggaagacc agcacgaatt tacaagttgt gctggtgctg agaccgtcaa
      601 atttctgatt cctggcaaaa aatatgtagc caatacagtt tttcattctg ttcttgctgg
      661 gctagtgtgt ggtcttggaa catggtattt gctcccaaac cgagtaacct tgctgtatgg
      721 cagccctggg gcgactgctg tgctctttgt gtttgggtgg ataacacttt gcataggaga
      781 atactcactc attgtaaaca ccgccacaga gacagcaaca ttccagaccc aggatactta
      841 cgaaatcact cctctcatga gaccacttta tatttttttc tttgtttctg tggaccttgc
      901 acacaggttt attgtaaata ttccagctct agagcaaatg aatcagattt tgcacatctt
      961 gtttgtgtta ctgcccttcc tgtgggcact tgggactctg cccccacctg acgcccttct
     1021 cttttgggca gtggagcagg ttttagagtt tggtcttggg ggttcatcca tgtccactca
     1081 cctacggtta ttggtcatgt tcatcgtttc tgctggagca gcagtcgtgt cgtacttcat
     1141 tcccagcacc gtcggagtgg ttctcttcat gactggactc gggttcttac tgagtcttaa
     1201 cccaagtgac gtgagcatgg tcttccggta cggtgtgacc aggcacaggg taggaagcag
     1261 tcatgaggct cgcccaggca attcaggcca ttggttcccg tggaaggagt gctttctgtt
     1321 catcgctgtg ctgggcatgg ctctcctgga ggctgggctg ctgcatcact atgtcagcgt
     1381 cccacaggtc tccagaagcg gcgctcaggc tgtcgtgggc tatgtcctga tggtgttact
     1441 ttcaatagtg tggatactga gagaaactca aagcatctac atctttggga ttttccgaaa
     1501 ccctttctat ccgaaggata tacacgctgt gtctgtattc tatgacaagc aaaacaagct
     1561 cctgaagatt ggtgctgtca gatggatttt gctaactcta gtgtcccctt tggcaatggt
     1621 ggccttcctt gcgctggaca gctccttaca cgggctccac tctgtgtctg tctccattgg
     1681 attcaccagg gctttcagaa tggtgtggca gaatacagaa actgctttac tggagactgt
     1741 ggttgtatca gcagtgcaca tggtgtccag cacagactgc tggtggaaca ggagccttga
     1801 tacaggaatc aggctgttat tgatcggtat catgcgagac cgtttgattc agttcatcac
     1861 taagttgcag tttgctgtga ctgtactttt ggcattgtgg acagaaaaaa aacagcatag
     1921 gaaaacaaca acgactttgt gtgtcctcaa cactgtattc gctcccttcg tgctgggtat
     1981 tatagttctt tcaacactac tgtcatctcc tctactgccc ctcttcacac ttcctgtttt
     2041 cttggtgggg tttcctcgtc cagttcagag ctggccagga gcagtgggca ctgcagcctg
     2101 tgtgtgcacc gacagtgtgt tctaccacca gatggtcccc aggctgactg cggcactgca
     2161 gaccgccatg gcggcgggaa gtttaggtct cctcctacct ggctctcatt acttgggccg
     2221 ttttgaggat cgtttaatat ggataatgat tctagagtgt ggctatactt attgctgcat
     2281 taatgttaag gggttagaac tgcaggaaac atcctgccac actgctgaag cccagagagt
     2341 tgatgaagtg tttgaaagtg cttttcagcg agaacaccca caagtgtgct cccttaatga
     2401 gcacctggag aatgtcttga cgccatgtac agttttgcct gtgaaactgt attctgatgc
     2461 caggaatgtg ctgactggca taattgattc tcctgacaac ctgaaggaat ttaaagatga
     2521 tcttattaag gtacttgtgt gggtgcttat tcagcactgc tctaaaaggc ccagcacgca
     2581 ggagaataaa actgaaaata cgggagaagc ctcaccagca ctgccaccgg ctgcgaacag
     2641 ctcaccatgc cccgagtccc tggaagactc tgaaagtgca aattcagact ggtctgatgg
     2701 tagtattttt gatgatgaac cagctatcaa aaacagaaaa gaaaaacttc agtccaaaga
     2761 tctaccttgt acaaagttac ctattccagg gtccgtagat tctcagaatc cagatgacca
     2821 ttctgcaggc acaggtccca aaaatgatct ttacaggacg gttatattgg gactccctgc
     2881 tgttgacaag ggacagcgag aagatgtggc atatattcct ctcgtggagt ttagttgttc
     2941 ccagtctcgc ttgctaagtt tacctgagga atggaggtct aactccacac cccgttccaa
     3001 gatcctagag atgagcgcct tgtttccaga agactggtac caatttgttt taaggcaatt
     3061 agaatgcttt cactcggaag agaagtcacg tgttctagag gaaattgcaa aggataaagc
     3121 tttaaaagac ttgtatgtcc atacagtgat ggcttgttat attggtttat ttggaataga
     3181 caatggagtg cctagccctg gtcagctagt aagagtctac aatggtggct taccctgggc
     3241 tggcaccttg gattggctct cagaaaaacc agagctgttc cacctagttc ggaaagcatt
     3301 caggtataca ctgaaactaa tggttgataa agcaagtttg ggtccaatag aagactttaa
     3361 agaactgacc aactgcctca gagagtacga gagagactgg tacattggct tagtgtctga
     3421 agagcagtgg aagagagcaa ttctagaaga aaagccatgc ttgttctgcc tggggtatga
     3481 gtctagtatg ggcgtttaca cctcgagggt gctgatgctt caggagatgt cggtgcacat
     3541 cgggaagctg aacgctgagg ccgttcgagg tcagtgggcc aacctctcgt gggaactgct
     3601 ttatgccacc aacgatgatg aggaacggta cagcatccag gctcacccac tgctgctgag
     3661 gaacctcact gtacaagccg ctgaccctcc cttgggatac ccaattttct cttcaaaacc
     3721 tctccctata catctgtgtt agttcatttt gacatccaaa tgcaggcaca tcagaggagg
     3781 ctctaactga aaagtaagag tgtgagtatt gtaatttcac tagactttct ctttgcccca
     3841 agaatgcctg agagaaaacc atatggtctg caaagctgtt tgtcagccgt tcaaatgact
     3901 cagacaacag gtcctctgtg tctaacgtgg gtaaaagtag ttggccaata tttgtgctgt
     3961 atgagttctg attggttttg gtagctagag atatttgtac acgattagat atttttttta
     4021 ctgctttgtt aaatgtctga aaagtggttt gtcttttaaa aagtcaacag gtatgtaatt
     4081 acatagttaa aaagacgttc atatcagtgt tttaaataat aatgaatctt tttgatgtta
     4141 acataacatg tatatatatt tatttgaaat gtaattaatt tttagtattt tcgaatgtac
     4201 tgttttagca tttaacatgg tgtgggcagt tccattcagt gtatccatga ttcatatact
     4261 tggcttggaa accgaaaaaa cttgaaataa aatctcacca gctaaatttt gttctttgtg
     4321 tttagagagg gatatccatg tgtataggtg cctacccagg cttgtggagg ccagaggcca
     4381 acctcaggca gtatctctct cagggacttg gacttgctga gttggctaga ctggccaacc
     4441 agtgagaccc atgctgtgtc cacctctgca gccagtgaga cccacactgt gtctacctcc
     4501 gcagcagtgg gataagaagc acacacctcg gtgcctggct ttctgcatga gggctggaga
     4561 acaacttgag gtcaccatgc ctagatggca agcactttac caactaagag accgtcccag
     4621 ccaaatttct ctcccacaaa tttctattat atataaatga aacagctctt cattcaccgt
     4681 gagtgttctc acaaccagaa gacatcccat agttctgact ccacatgcaa taatgaatta
     4741 cccagtggga ccatcagggc tctgagtatt tgagctgaaa cttggaagta atgggaaatt
     4801 tccttaaagt ggtaagagtt tcatcattat cctctcgctg cctagaaatg agttacagtt
     4861 acctggaggg aaggaagacg atgatgtaag cctctgcaga tgggaacctg ctttattcct
     4921 tactcgattt ctaactaaac cgccaagaac tcagagaatc tcaagatgat gatgcatttt
     4981 ctttagaaat gactgagctt gctgggcagt ggtggatcac acctttaatc ccagcacttg
     5041 ggaggtagag gcagaaagat ttctgagttt gaagccagcc tggtctacag gtctacagag
     5101 taagttccag tacttcaggg ctacagagaa accctgtctc ggaaaaagaa aagaaaagag
     5161 aagagaagag aagagaagag aagagaagag aagagaagag aagagaagag aagagaagag
     5221 aagagaagag agaagagaaa agagaaaaga aaagaaaaga aaagaaaaga aaagaaaaga
     5281 aaagaaaaag aaaagagaga ggaggggagg ggaggggagg ggaggagagg agaggagagg
     5341 agaggagagg agaggagagg agaggagagg agagggaaga ggagagaaga gaaagaggag
     5401 aagggaagag aagaggatag gagaggagag aggagaggag aaagagaaga gaagagaaga
     5461 gaagagaaga gaagagaaga gaagagaaga gaagagaaga gaagagaaga gaagagaaga
     5521 gaagagaaga gaagagaaga gaagagaaga agagaaaaga gaaaaagaaa tgactgagct
     5581 ggttatagtt tgtgattagc cccccttggc cataccccct tggcctcacc tcccttggtt
     5641 ttgtattatt ctaaaataat cacatagtta ccaatctcat tttttgtttt ttgattcttg
     5701 gggttttttt ttgggggggg ggggttggat atttttggtt gcttgtttgg tttggtttgg
     5761 tttgactttt ggtttgttgg tttttctcaa ttgtttgttt tagtgatttg aattttttta
     5821 actttgtatt gattttttgt gaacttcaca tcatgcaccc gagtcccact catctcccca
     5881 cccttctata tttatcctcc acccttgcaa tctctccttc aaaagaaaaa taataaaaat
     5941 ttaaaaataa aaccatcttg ctgtggaa
//
