LOCUS       NM_001407215            4279 bp    mRNA    linear   ROD 28-MAY-2024
DEFINITION  Mus musculus protein-L-isoaspartate (D-aspartate)
            O-methyltransferase 1 (Pcmt1), transcript variant 31, mRNA.
ACCESSION   NM_001407215
VERSION     NM_001407215.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ling,Z., Zhou,S., Zhou,Y., Zhong,W., Su,Z. and Qin,Z.
  TITLE     Protective role of madecassoside from Centella asiatica against
            protein L-isoaspartyl methyltransferase deficiency-induced
            neurodegeneration
  JOURNAL   Neuropharmacology 246, 109834 (2024)
   PUBMED   38181970
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Kalailingam,P., Mohd-Kahliab,K.H., Ngan,S.C., Iyappan,R.,
            Melekh,E., Lu,T., Zien,G.W., Sharma,B., Guo,T., MacNeil,A.J.,
            MacPherson,R.E., Tsiani,E.L., O'Leary,D.D., Lim,K.L., Su,I.H.,
            Gao,Y.G., Richards,A.M., Kalaria,R.N., Chen,C.P., McCarthy,N.E. and
            Sze,S.K.
  TITLE     Immunotherapy targeting isoDGR-protein damage extends lifespan in a
            mouse model of protein deamidation
  JOURNAL   EMBO Mol Med 15 (12), e18526 (2023)
   PUBMED   37971164
REFERENCE   3  (bases 1 to 4279)
  AUTHORS   Zhang,C., Guo,Z.F., Liu,W., Kazama,K., Hu,L., Sun,X., Wang,L.,
            Lee,H., Lu,L., Yang,X.F., Summer,R. and Sun,J.
  TITLE     PIMT is a novel and potent suppressor of endothelial activation
  JOURNAL   Elife 12, e85754 (2023)
   PUBMED   37070640
  REMARK    GeneRIF: PIMT is a novel and potent suppressor of endothelial
            activation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4279)
  AUTHORS   Qin,Z., Dimitrijevic,A. and Aswad,D.W.
  TITLE     Accelerated protein damage in brains of PIMT+/- mice; a possible
            model for the variability of cognitive decline in human aging
  JOURNAL   Neurobiol Aging 36 (2), 1029-1036 (2015)
   PUBMED   25465735
  REMARK    GeneRIF: PIMT levels may significantly influence the course of
            age-related central nervous system dysfunction.
REFERENCE   5  (bases 1 to 4279)
  AUTHORS   Dimitrijevic,A., Qin,Z. and Aswad,D.W.
  TITLE     Isoaspartyl formation in creatine kinase B is associated with loss
            of enzymatic activity; implications for the linkage of isoaspartate
            accumulation and neurological dysfunction in the PIMT knockout
            mouse
  JOURNAL   PLoS One 9 (6), e100622 (2014)
   PUBMED   24955845
  REMARK    GeneRIF: Loss of normal CKB structure and function contributes to
            the mechanisms by which isoaspartate accumulation leads to central
            nervous system dysfunction in the PIMT-Knockout mouse.
            Erratum:[PLoS One. 2014;9(12):e116556]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4279)
  AUTHORS   MacLaren,D.C. and Clarke,S.
  TITLE     Rapid mapping of genomic P1 clones: the mouse
            L-isoaspartyl/D-aspartyl methyltransferase gene
  JOURNAL   Genomics 35 (2), 299-307 (1996)
   PUBMED   8661142
REFERENCE   7  (bases 1 to 4279)
  AUTHORS   Galus,A., Lagos,A., Romanik,E.A. and O'Connor,C.M.
  TITLE     Structural analysis of transcripts for the protein L-isoaspartyl
            methyltransferase reveals multiple transcription initiation sites
            and a distinct pattern of expression in mouse testis:
            identification of a 5'-flanking sequence with promoter activity
  JOURNAL   Arch Biochem Biophys 312 (2), 524-533 (1994)
   PUBMED   8037467
REFERENCE   8  (bases 1 to 4279)
  AUTHORS   Warden,C.H., Mehrabian,M., He,K.Y., Yoon,M.Y., Diep,A., Xia,Y.R.,
            Wen,P.Z., Svenson,K.L., Sparkes,R.S. and Lusis,A.J.
  TITLE     Linkage mapping of 40 randomly isolated liver cDNA clones in the
            mouse
  JOURNAL   Genomics 18 (2), 295-307 (1993)
   PUBMED   8288233
REFERENCE   9  (bases 1 to 4279)
  AUTHORS   MacLaren,D.C., O'Connor,C.M., Xia,Y.R., Mehrabian,M., Klisak,I.,
            Sparkes,R.S., Clarke,S. and Lusis,A.J.
  TITLE     The L-isoaspartyl/D-aspartyl protein methyltransferase gene (PCMT1)
            maps to human chromosome 6q22.3-6q24 and the syntenic region of
            mouse chromosome 10
  JOURNAL   Genomics 14 (4), 852-856 (1992)
   PUBMED   1478665
REFERENCE   10 (bases 1 to 4279)
  AUTHORS   Romanik,E.A., Ladino,C.A., Killoy,L.C., D'Ardenne,S.C. and
            O'Connor,C.M.
  TITLE     Genomic organization and tissue expression of the murine gene
            encoding the protein beta-aspartate methyltransferase
  JOURNAL   Gene 118 (2), 217-222 (1992)
   PUBMED   1511895
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC156391.6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14995048.2119050.1,
                                           SRR17253011.1196778.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-522               AC156391.6         112794-113315
            523-627             AC156391.6         127235-127339
            628-659             AC156391.6         128769-128800
            660-764             AC156391.6         131961-132065
            765-885             AC156391.6         135575-135695
            886-971             AC156391.6         136260-136345
            972-1147            AC156391.6         138165-138340
            1148-4279           AC156391.6         145414-148545
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 2.26 cM"
     gene            1..4279
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="protein-L-isoaspartate (D-aspartate)
                     O-methyltransferase 1"
                     /db_xref="GeneID:18537"
                     /db_xref="MGI:MGI:97502"
     exon            1..522
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     CDS             468..1160
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /EC_number="2.1.1.77"
                     /note="isoform 11 is encoded by transcript variant 31;
                     protein-L-isoaspartate(D-aspartate) O-methyltransferase;
                     protein-beta-aspartate methyltransferase; L-isoaspartyl
                     protein carboxyl methyltransferase; protein
                     L-isoaspartyl/D-aspartyl methyltransferase"
                     /codon_start=1
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase isoform 11"
                     /protein_id="NP_001394144.1"
                     /db_xref="GeneID:18537"
                     /db_xref="MGI:MGI:97502"
                     /translation="MAWKSGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYAKSN
                     PYMDSPQSIGFQATISAPHMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGN
                     SGKVIGIDHIKELVDDSITNVKKDDPMLLSSGRVRLVVGDGRMGYAEEAPYDAIHVGA
                     AAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSVKMKPLMGVIYVPLTD
                     KEKQWSRWKDEL"
     exon            523..627
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            628..659
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            660..764
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            765..885
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            886..971
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1147
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..4279
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1414..1419
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="hexamer: AAGAAA"
     polyA_site      1439
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="major polyA site"
     regulatory      2727..2732
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="hexamer: AATAAA"
     polyA_site      2744
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="major polyA site"
     regulatory      4257..4262
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
                     /note="hexamer: AATAAA"
     polyA_site      4279
                     /gene="Pcmt1"
                     /gene_synonym="PIMT"
ORIGIN      
        1 gcggagccca agaaggcggc acacgtcgga cccgccatcc cgcatcagcc agagccgcgg
       61 ctaggaagga gcctcatcac gcagaacacc agctcgccgg ccgcaggacg gaggcaggtg
      121 caggcctcag gaaggggaac tgagcagcgt tcgtttgtgc gacctcgctg cggccaccga
      181 gcactggtta tcgcccccgc tcgctactgg gctccaggac tcccccgccg cgggccccgc
      241 ctggccgcac cccggactgc gcggcgtcac agagcgcatg cgtgccgcgg gggatgccgg
      301 gagcgcgcat tggcggcagt ggcagcgacg gcagtaacag cggccgctcg agcggggaca
      361 ccagcggggc ggtgaccgtg tgggaggtgg tctcactctt ggggaaactg ctagggaccg
      421 tcgccgcgct gaaggtggtt ctgtacctgc tccgggtgtg cttcgcgatg gcctggaaat
      481 ccggcggcgc cagccactcg gagctaatcc acaacctccg caagaatgga atcatcaaga
      541 cagataaagt atttgaagtg atgctggcta cagaccgttc ccactatgca aaatctaacc
      601 cttacatgga ttctccacaa tcaataggtt tccaggcaac aatcagtgcc cctcatatgc
      661 acgcatatgc actagaactt ctgtttgacc agctgcatga aggtgctaaa gccctggacg
      721 tgggctctgg aagtggaatc ctcaccgcct gttttgcacg gatggttgga aatagtggaa
      781 aagtcattgg aattgatcac attaaagaac tagtagatga ctcaataact aatgtcaaaa
      841 aggatgaccc aatgctcctg tcctcggggc gagtgcggct ggtggtgggt gatggaagaa
      901 tgggatacgc tgaagaagcc ccttacgacg ctattcatgt gggagctgca gccccagttg
      961 tgccccaggc attaatagac cagttaaagc ctggtggaag attgatattg ccagtcggtc
     1021 ctgcaggagg aaaccaaatg ttggagcagt atgacaagct acaagatggc agtgtcaaaa
     1081 tgaaacctct catgggggtg atatacgtgc ctttaacaga taaagaaaag cagtggtcca
     1141 ggtggaagga tgaactgtga aagcaacgtg agcttgacca gtctataaac cgcagtgggc
     1201 cgctcctctc ggtccttaca gcatttgaga accaacagca tcacagcttg ttttggactt
     1261 tgttacactc ttactttcag catgaaaacg tgtggttttg taggatttcc taattccaaa
     1321 cggcgcggag ccaaactgat agaggaaggc tgcagagcgt taactgcgtg tgttgtttta
     1381 acatgcccac atttcacttt aaaaaaatat taaaagaaag agttctccaa ctggaaaaat
     1441 tagttttgaa gagtggcttt gcttttcttg ggcacttaat tctgttccaa tattaattca
     1501 tcagatttat tttgtgcatc agataacacc acattcacaa gtttagcttc tttggtagcc
     1561 aatgtctgtt agatgctact aagaaaacag agatgagctt tcttcttaaa agcctttgat
     1621 gtggcgtcat aggatagcat gtcgtagata caatcagctg ctgttacctt aaaactaggt
     1681 gtttgtaaat actgaaactt gagacgacga tgatggcagg tggtgtccca aaaacactca
     1741 gctgttccga ttggaaattt tgatgtaaat ttcctctctc taaaaatgct aggaggcttg
     1801 tagctcaata tcatttattt gtttctagtt tgctgctggt aatgtatctg catctaacat
     1861 tctgtgtaag cggtcgtgtc tttgcacagt ttatgcagcg ctgctttgcc aaataaaatt
     1921 aaaagcaaaa gaagcattgg tggcctttga atctagaagg acacaggtct gctaggtcca
     1981 gatgctgccc acagtcctgg ggaagcgtcg tagtcttatg atattctagg aggtagatat
     2041 gcatagcatt cgtccactct atgtggctgc atttcttcag gatggagact ctgtgttcat
     2101 tgtttctctc tgccttccta gcccaaggct tgctttttaa tagctctgta gtaaatgctc
     2161 aatgagtgac tgggtgaatg tagtatgagg aagaatgtgg ttgagagtaa acatactgaa
     2221 atccatgtct catctataaa tagattcatt aatttaataa ctgtaaaatg tctgtgaagg
     2281 caatggcact ttgaagatct tatttaaata tggttttata ttttctaata agactttagt
     2341 tttttgtgaa gatcctagaa cttgagagaa gattagaagc tcccggtaaa tggggctaag
     2401 caaagcgaag ccttccctct atgtgtaagg gcacatgatt ctgacaagaa gactcttagt
     2461 gtctgagaag cccatcccca ctcttcacat cttaaattac tgatttaaaa gtcatgcaga
     2521 catcacctga caagtgtctc ttcttggctt aggtctcagt cccaggaaac tagcattact
     2581 gtggtcccgc agacttgaaa accttaacca gctttggttt gctgctggtt gttgctttgg
     2641 aatcctgtca gtgaccccct ggagtccagg atgcatggtc atttgaaata tgtgtaaagt
     2701 caaatgttca ctcaggcttt aaagcaaata aattcttaaa cttacccacc agtctggatc
     2761 cttggtagtc attgcacatt acagcacgca aatctctgtg ctgggcttca ggacggaaga
     2821 ggtgcttcca ggtcagtttg ccgtccagtg gcggggacag tttataaata ggcaccatac
     2881 acttcaccca gtttgtacag aaactctgtg cccaagcctt gctcttagac ttgacacttt
     2941 taggaggtga aactttagga tgttgaatga acagccagtt tgaacagcag cactaagtgg
     3001 gtagagatag aagaatggtg ttaatgaggt ctaagccctt taaaagagtt gaatttatat
     3061 atgtgtttaa gacaggtaag aaatgcctga ttttcagtgt gcataatttc cagagcattt
     3121 tattttcctc atagaaagta catctgaata cacataatgt aaagggcaat gaagatactt
     3181 cgagtcatta taggtagtgt gatcttgtga ataaactctt cctataaatt tgctcttcct
     3241 agaagctaaa tctagttgtc agacttgggt tgcagcaata agcttatgtc agggttgagt
     3301 ttctcttcag ggctgcttgc tctttcccag ccaggaacag gacaaatcgg cacccaggga
     3361 aataactact actggccaca ccaatactta ggacttcatc ccccttgcag ctgccatgtt
     3421 ttattgtttg tttgtttatt tgtggcaggt gggggtggat agcaagatgg ctcggggtaa
     3481 agaggcttgc tgccaaacct gaagacctga gtttcacccc tcccccctcc cagacccaca
     3541 gagtagaagg acagctgaca cctgcagatt gttctctgac cttcaggcat ggtagcactt
     3601 gcctgaaatc ccagcacttg gaagcaggaa gggtcagaag tcatcctagg ttacacaagt
     3661 tcaaggccag cccgaatttc atgagagcct gccttaaagg aaagaaaaaa ccaaaaagtt
     3721 tctacagtgc atctcttcac taaagcatcg taagcattga gtggattcta aaggagccat
     3781 ttttaaagta ataactttgt aaactcttca agctgttctt gaaggaattc ctcagagtgt
     3841 ttaaggcctt ttatctctct agagttttct aatttctggt agtttagtgt ggcatcaaag
     3901 aaagtcatga cagtgttgtc acccagcact gatagacaaa tagtaagcat gagtcatctt
     3961 ataaattgtt gtagaactca accggctgat aacttatttt aggtaagcac ttaaactctt
     4021 gtagcataca gatcatttct tatcgaatgc tgtgttcctg atgccctaat atcttaatgt
     4081 attctgtagt ggagatgggc cctgactgga ctttggttgc atctctgagc ttccaccatc
     4141 tttcagaaag atggtagtat tttgccctta gcattgttaa aggttctcac tcagtaaggg
     4201 gacccacttg aaaagggtac tggcccaagg aagcctggga ctgtgcactg gaatataata
     4261 aagtggaaat gaagtctaa
//
