LOCUS       NM_011995              20099 bp    mRNA    linear   ROD 16-OCT-2024
DEFINITION  Mus musculus piccolo (presynaptic cytomatrix protein) (Pclo),
            transcript variant 1, mRNA.
ACCESSION   NM_011995
VERSION     NM_011995.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 20099)
  AUTHORS   Pulcrano,S., De Gregorio,R., De Sanctis,C., Volpicelli,F.,
            Piscitelli,R.M., Speranza,L., Perrone-Capano,C., di Porzio,U.,
            Caiazzo,M., Martini,A., Giacomet,C., Medina,D., Awatramani,R.,
            Viggiano,D., Federici,M., Mercuri,N.B., Guatteo,E. and
            Bellenchi,G.C.
  TITLE     miR-218 Promotes Dopaminergic Differentiation and Controls Neuron
            Excitability and Neurotransmitter Release through the Regulation of
            a Synaptic-Related Genes Network
  JOURNAL   J Neurosci 43 (48), 8104-8125 (2023)
   PUBMED   37816598
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20099)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 20099)
  AUTHORS   Cozzolino,F., Vezzoli,E., Cheroni,C., Besusso,D., Conforti,P.,
            Valenza,M., Iacobucci,I., Monaco,V., Birolini,G., Bombaci,M.,
            Falqui,A., Saftig,P., Rossi,R.L., Monti,M., Cattaneo,E. and
            Zuccato,C.
  TITLE     ADAM10 hyperactivation acts on piccolo to deplete synaptic vesicle
            stores in Huntington's disease
  JOURNAL   Hum Mol Genet 30 (13), 1175-1187 (2021)
   PUBMED   33601422
  REMARK    GeneRIF: ADAM10 hyperactivation acts on piccolo to deplete synaptic
            vesicle stores in Huntington's disease.
REFERENCE   4  (bases 1 to 20099)
  AUTHORS   Li,P., Lin,Z., An,Y., Lin,J., Zhang,A., Wang,S., Tu,H., Ran,J.,
            Wang,J., Liang,Y., Liu,Z., Ye,C., Fu,X. and Gao,J.
  TITLE     Piccolo is essential for the maintenance of mouse retina but not
            cochlear hair cell function
  JOURNAL   Aging (Albany NY) 13 (8), 11678-11695 (2021)
   PUBMED   33882456
  REMARK    GeneRIF: Piccolo is essential for the maintenance of mouse retina
            but not cochlear hair cell function.
REFERENCE   5  (bases 1 to 20099)
  AUTHORS   Kaplan,M.M. and Flucher,B.E.
  TITLE     Postsynaptic CaV1.1-driven calcium signaling coordinates
            presynaptic differentiation at the developing neuromuscular
            junction
  JOURNAL   Sci Rep 9 (1), 18450 (2019)
   PUBMED   31804576
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 20099)
  AUTHORS   Collins,M.O., Yu,L., Coba,M.P., Husi,H., Campuzano,I.,
            Blackstock,W.P., Choudhary,J.S. and Grant,S.G.
  TITLE     Proteomic analysis of in vivo phosphorylated synaptic proteins
  JOURNAL   J Biol Chem 280 (7), 5972-5982 (2005)
   PUBMED   15572359
REFERENCE   7  (bases 1 to 20099)
  AUTHORS   Fujimoto,K., Shibasaki,T., Yokoi,N., Kashima,Y., Matsumoto,M.,
            Sasaki,T., Tajima,N., Iwanaga,T. and Seino,S.
  TITLE     Piccolo, a Ca2+ sensor in pancreatic beta-cells. Involvement of
            cAMP-GEFII.Rim2. Piccolo complex in cAMP-dependent exocytosis
  JOURNAL   J Biol Chem 277 (52), 50497-50502 (2002)
   PUBMED   12401793
  REMARK    GeneRIF: binds to cAMP-GEFII and forms both homodimer and
            heterodimer with Rim2 in a Ca(2+)-dependent manner, whereas Rim2
            alone does not form the homodimer
REFERENCE   8  (bases 1 to 20099)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Hara,Y., Nagase,T.,
            Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: I. The complete nucleotide sequences of 100 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 9 (5), 179-188 (2002)
   PUBMED   12465718
REFERENCE   9  (bases 1 to 20099)
  AUTHORS   Gerber,S.H., Garcia,J., Rizo,J. and Sudhof,T.C.
  TITLE     An unusual C(2)-domain in the active-zone protein piccolo:
            implications for Ca(2+) regulation of neurotransmitter release
  JOURNAL   EMBO J 20 (7), 1605-1619 (2001)
   PUBMED   11285225
REFERENCE   10 (bases 1 to 20099)
  AUTHORS   Wang,X., Kibschull,M., Laue,M.M., Lichte,B., Petrasch-Parwez,E. and
            Kilimann,M.W.
  TITLE     Aczonin, a 550-kD putative scaffolding protein of presynaptic
            active zones, shares homology regions with Rim and Bassoon and
            binds profilin
  JOURNAL   J Cell Biol 147 (1), 151-162 (1999)
   PUBMED   10508862
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC144625.3, AC125043.4, AC125533.4, AC144480.5 and AI852499.1.
            
            On Nov 20, 2007 this sequence version replaced NM_011995.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN01164131,
                              SAMN01164132 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-543               AC144625.3         20198-20740         c
            544-2026            AC144625.3         13334-14816         c
            2027-3418           AC125043.4         163996-165387       c
            3419-4129           AC125043.4         35122-35832         c
            4130-9188           AC125043.4         24784-29842         c
            9189-11206          AC125043.4         22410-24427         c
            11207-13388         AC125533.4         52700-54881
            13389-13525         AC125533.4         59140-59276
            13526-13616         AC125533.4         63517-63607
            13617-13742         AC125533.4         90762-90887
            13743-13851         AC125533.4         105437-105545
            13852-13919         AC125533.4         106082-106149
            13920-14134         AC125533.4         107010-107224
            14135-14185         AC125533.4         110579-110629
            14186-14310         AC125533.4         115603-115727
            14311-14337         AC125533.4         118822-118848
            14338-14431         AC125533.4         128681-128774
            14432-14503         AC125533.4         130643-130714
            14504-14684         AC125533.4         132195-132375
            14685-14879         AC125533.4         134115-134309
            14880-15021         AC125533.4         151893-152034
            15022-15089         AC125533.4         157550-157617
            15090-15224         AC144480.5         152589-152723       c
            15225-15370         AC144480.5         151217-151362       c
            15371-20077         AC144480.5         144814-149520       c
            20078-20099         AI852499.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..20099
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 6.27 cM"
     gene            1..20099
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /note="piccolo (presynaptic cytomatrix protein)"
                     /db_xref="GeneID:26875"
                     /db_xref="MGI:MGI:1349390"
     exon            1..543
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     CDS             305..15511
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /note="isoform 1 is encoded by transcript variant 1;
                     aczonin; protein piccolo; brain-derived HLMN protein;
                     multidomain presynaptic cytomatrix protein"
                     /codon_start=1
                     /product="protein piccolo isoform 1"
                     /protein_id="NP_036125.4"
                     /db_xref="CCDS:CCDS51415.1"
                     /db_xref="GeneID:26875"
                     /db_xref="MGI:MGI:1349390"
                     /translation="MGNEASLEGEGLPEGLAAAAGGAGGSGSALHPGIPAGMEADLSQ
                     LSEEERRQIAAVMSRAQGLPKGSVPAAAAESPSMHRKQELDSSQAPQQPGKPPDPGRP
                     PQHGLSKSRTTDTFRSEQKLPGRSPSTISLKESKSRTDFKEEYKSSMMPGFFSDVNPL
                     SAVSSVVNKFNPFDLISDSEAVQEETTKKQKVAQKDQGKSEGITKPSLQQPSPKLIPK
                     QQGPGKEVIPQDIPSKSVSSQQAEKTKPQAPGTAKPSQQSPAQTPAQQAKPVAQQPGP
                     AKATVQQPGPAKSPAQPAGTGKSPAQPPVTAKPPAQQAGLEKTSLQQPGPKSLAQTPG
                     QGKVPPGPAKSPAQQPGTAKLPAQQPGPQTAAKVPGPTKTPAQLSGPGKTPAQQPGPT
                     KPSPQQPIPAKPQPQQPVATKPQPQQPAPAKPQPQHPTPAKPQPQHPTPAKPQPQQPT
                     PAKPQPQQPTPAKPQPQQPTPAKPQPQHPTPAKPQPQQPGLGKPSAQQPSKSISQTVT
                     GRPLQAPPTSAAQAPAQGLSKTICPLCNTTELLLHTPEKANFNTCTECQSTVCSLCGF
                     NPNPHLTEIKEWLCLNCQMQRALGGELAAIPSSPQPTPKAASVQPATASKSPVPSQQA
                     SPKKELPSKQDSPKAPESKKPPPLVKQPTLHGPTPATAPQPPVAEALPKPAPPKKPSA
                     ALPEQAKAPVADVEPKQPKTTETLTDSPSSAAATSKPAILSSQVQAQAQVTTAPPLKT
                     DSAKTSQSFPPTGDTITPLDSKAMPRPASDSKIVSHPGPTSESKDPVQKKEEPKKAQT
                     KVTPKPDTKPVPKGSPTPSGTRPTTGQATPQSQQPPKPPEQSRRFSLNLGGIADAPKS
                     QPTTPQETVTGKLFGFGASIFSQASNLISTAGQQAPHPQTGPAAPSKQAPPPSQTLAA
                     QGPPKSTGPHPSAPAKTTAVKKETKGPAAENLEAKPVQAPTVKKAEKDKKPPPGKVSK
                     PPPTEPEKAVLAQKPDKTTKPKPACPLCRTELNVGSQDPPNFNTCTECKNQVCNLCGF
                     NPTPHLTEIQEWLCLNCQTQRAISGQLGDMDKMPPASSGPKASPVPAPAEPPPQKTPT
                     AAHAKGKKKETEVKAETEKQIPEKETPSIEKTPPAVATDQKLEESEVTKSLVSVLPEK
                     KPSEEEKALPADKKEKKPPAAEAPPLEEKKPIPDDQKLPPDAKPSASEGEEKRDLLKA
                     HVQIPEEGPIGKVASLACEGEQQPDTRPEDLPGATPQTLPKDRQKESRDVTQPQAEGT
                     AKEGRGEPSKDRTEKEEDKSDTSSSQQPKSPQGLSDTGYSSDGISGSLGEIPSLIPSD
                     EKDLLKGLKKDSFSQESSPSSPSDLAKLESTVLSILEAQASTLVGEKAEKKTQPQKVS
                     PEQPQDQQKTQTPSETRDISISEEEIKESQEKKVTSKKDSAQGFPSRKEHKENPELVD
                     DLSPRRASYDSVEDSSESENSPVARRKRRTSIGSSSSEEYKQEDSQGSGEDEDFIRKQ
                     IIEMSADEDASGSEDEEFIRSQLKEIGGVTESQKREETKGKGKSPAGKHRRLTRKSST
                     SFDDDAGRRHSWHDEDDETFDESPELKFRETKSQESEELVVAGGGGLRRFKTIELNST
                     VTDKYSAESSQKKTTLYFDEEPELEMESLTDSPEDRSRGEGSSSLHASSFTPGTSPTS
                     VSSLDEDSDSSPSHKKGESKQQRKARHRSHGPLLPTIEDSSEEEELREEEELLKEQEK
                     QRELEQQQRKSSSKKSKKDKDELRAQRRRERPKTPPSNLSPIEDASPTEELRQAAEME
                     ELHRSSCSEYSPSIESDPEGFEISPEKIIEVQKVYKLPTAVSLYSPTDEQSVMQKEGA
                     QKALKSAEEMYEEMMHKPHKYKAFPAANERDEVFEKEPLYGGMLIEDYIYESLVEDTY
                     NGSVDGSLLTRQDEQNGFMQQRGREQKIRLQEQIYDDPMQKITDLQKEFYELESLHSI
                     VPQEDIVSSSYIIPESHEIVDLGSMVTSTSEEKKLLDADAAYEELMKRQQMQVTDGSS
                     LIQTTMGDDMAESTLDFDRVQDASLTSSILSGASLTDSTSSATLSIPDVKITQHFSTE
                     EFEDEYVTDYTREIQEIIAHESLILTYSEPSESATSVPPSDTPSLTSSISSVCTTDSS
                     SPVTTLDSLTTVYTEPADVITKFKDSEEISSTYFPGSVIDYPEDIGVSLDRTITPESR
                     TNADQIMISFPGIAPSITESVATKPERPQADTISTDLPISEKELIKGKKETGDGIILE
                     VLDAYKDKREESEAELTKISLPETGLAPTPSSQTKEQPGSPHSVSGEILGQEKPTYRS
                     PSGGLPVSTHPSKSHPFFRSSSLDISAQPPPPPPPPPPPPPPPPPPPPPPLPPATSPK
                     PPTYPKRKLAAAAPVAPTAIVTAHADAIPTVEATAARRSNGLPATKICAAAPPPVPPK
                     PSSIPTGLVFTHRPEASKPPIAPKPAVPEIPVTTQKTTDTCPKPTGLPLTSNMSLNLV
                     TSADYKLPSPTSPLSPHSNKSSPRYSKSLMETYVVITLPSEPGTPTDSSAAQAITSWP
                     LGSPPKDLVSLETVFSVVPPMTSTEIPSASQPTLYTSGALGTFSVTPAVTASLFQTVP
                     TSLTQFLPAEASKPEVSAVSSAVPSVAPRSVSIPIPPEPLALDRHQYKENGKLPLIGD
                     AIDLRTIPKSEVKVTEKCMDLSASAMDVKRQTTANEVYRRQISAVQPSIINLSAASSL
                     GTPVTMDSKTVAVVTCTDTTIYTTGTESQVGIEHAVTSPLQLTTSKHTELQYRKPSSQ
                     AFPMIRDEAPINLSLGPSTQAVTLAVTKPVTVPPVGVTNGWTDSTISQGITDGEVVDL
                     STSKSHRTVVTMDESTSNVVTKIIEDEEKPVDLTAGRRAVCCDMVYKLPFGRSCTAQQ
                     PATTLPEDRFGYRDDHYQYDRSGPYGYRGIGGMKPSMSDTNLAEAGHFFYKSKNAFDY
                     SGGTEAAVDLTSGRVSTGEVMDYSSKTTGPYPETRQVISGVGISTPQYSTARMTPPPG
                     PQYGVGSVLRSSNGVVYSSVATPIPSTFAITTQPGSIFSTTVRDLSGIHTTDAITSLS
                     ALHQSQPMPRSYFITTGASETDISVTSIDINASLQTITMETLPAETMDSVPTLTTASE
                     VFSEVVGEESTLLIVPDEDKQQQQLDLERELLELEKIKQQRFAEELEWERQEIQRFRE
                     QEKIMVQKKLEELQSMKQHLLYQQEEERQAQFMMRQETLAQQQLQLEQIQQLQQQLHQ
                     QLEEQKLRQIYQYNYEPSGTASPQTTTEQAILEGQYVATEGSQFWATEDATTTASTVV
                     AIEIPQSQGWYTVQSDGVTQYIAPPGILSTVSEIPLTDVVVKEEKQPKKRSSGAKVRG
                     QYDEMGESMADDPRNLKKIVDSGVQTDDEETADRTYASRRRRTKKSVDTSVQTDDEDQ
                     DEWDMPSRSRRKARTGKYGDSTAEGDKTKPPSKVSSVAVQTVAEISVQTEPLGTIRTP
                     SIRARVDAKVEIIKHISAPEKTYKGGSLGCQTETDPDTQSPPYMGATSPPKDKKRPTP
                     LEIGYSSSHLRADPTVQLAPSPPKSPKVLYSPISPLSPGHALEPAFVPYEKPLPDDIS
                     PQKVLHPDMAKVPPASPKTAKMMQRSMSDPKPLSPTADESSRAPFQYSEGFTAKGSQT
                     TSGTQKKVKRTLPNPPPEEASTGTQSTYSTMGTASRRRMCRTNTMARAKILQDIDREL
                     DLVERESAKLRKKQAELDEEEKEIDAKLRYLEMGINRRKEALLKEREKRERAYLQGVA
                     EDRDYMSDSEVSSTRPSRVESQHGIERPRTAPQTEFSQFIPPQTQTEAQLVPPTSPYT
                     QYQYSSPALPTQAPTPYTQQSHFQQQTLYHQQVSPYQTQPTFQAVATMSFTPQAQPTP
                     TPQPSYQLPSQMMVIQQKPRQTTLYLEPKITSTYEVIRNQPLMIAPVSTDNTYAVSHL
                     GSKYNSLDLRIGLEERSSMASSPISSISADSFYADIDHHTSRNYVLIDDIGDITKGTA
                     ALSSAFSLHEKDLSKTDRLLRTTETRRSQEVTDFLAPLQTSSRLHSYVKAEEDSMEDP
                     YELKLLKHQIKQEFRRGTESLDHLAGLSHYYHADTSYRHFPKSEKYSISRLTLEKQAA
                     KQLPAAILYQKQSKHKKALIDPKMSKFSPIQESRDLEPDYPTYLSSSTSSIGGISSRA
                     RLLQDDITFGLRKNITDQQKFMGSSLGSGLGTLGNTIRSALQDEADKPYSSGSRSRPS
                     SRPSSVYGLDLSIKRDSSSSSLRLKAQEAEALDVSFGHSSSSARTKPTSLPISQSRGR
                     IPIVAQNSEEESPLSPVGQPMGMARAAAGPLPPISADTRDQFGSSHSLPEVQQHMREE
                     SRTRGYDRDIAFIMDDFQHAMSDSEAYHLRREETDWFDKPRESRLENGHGLDRKLPER
                     LVHSRPLSQHQEQILQMNGKTMHYIFPHARIKITRDSKDHTVSGNGLGIRIVGGKEIP
                     GHSGEIGAYIAKILPGGSAEHSGKLIEGMQVLEWNGIPLTSKTYEEVQSIINQQSGEA
                     EICVRLDLNMLSDSENPQHLELHEPPKVVDKAKSPGVDPKQLAAELQKVSLQQSPLVM
                     SSVVEKGAHAHSGPTSAGSSSVPSPGQPGSPSVSKKKHGGSKPTDVSKTASHPITGEI
                     QLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGQVMVVQNASVEYKRR
                     TKYVQKSLNPEWNQTVIYKSISMEQLMKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTS
                     HLDNTPRWYPLKEQTESIEHGKSHSSQNSQQSPKPSVIKSRSHGIFPDPSKDMQVPTI
                     EKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVAQTHLEDAGAAIAAAEAAVQQLRIQP
                     TKPTNHRPAETSVSTGSSGSSVGSGYSVDSEGSSCVAGEPNLLPIPRIGKMGQNGQDP
                     VKQPGMGAADTEAKTQVMGEIKLALKKEMKTDGEQLIVEILQCRNITYKFKSPDHLPD
                     LYVKIYVINIATQKKVIKKKTRVCRHDREPSFNETFRFSLSPAGHSLQILLFSNGGKF
                     MKKTLIGEACIWLDKVDLRKRIVNWHKLLMSPTQTH"
     exon            544..2026
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..3418
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            3419..4129
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            4130..9188
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            9189..11206
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            11207..13388
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            13389..13525
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            13526..13616
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            13617..13742
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            13743..13851
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            13852..13919
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            13920..14134
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            14135..14185
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            14186..14310
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            14311..14337
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            14338..14431
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            14432..14503
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            14504..14684
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            14685..14879
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            14880..15021
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            15022..15089
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            15090..15224
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            15225..15370
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     exon            15371..20084
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /inference="alignment:Splign:2.1.0"
     regulatory      16515..16520
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /note="hexamer: ATTAAA"
     polyA_site      16537
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /note="major polyA site"
     regulatory      19909..19914
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /note="hexamer: AATAAA"
     polyA_site      19936
                     /gene="Pclo"
                     /gene_synonym="Acz; Pico"
                     /note="major polyA site"
ORIGIN      
        1 gcggggcgcg gggcggggcc gtcggcgcgg ggctggccga gctccgctgc ttctgggctg
       61 ctccggcgct gaggaggcaa ccgggagaca gggcgtgaga agcttccctg gcgctgtcca
      121 acatggagga gctgcccggc gccggcacag cctgcggact ggtctgagct cgccccactt
      181 ccccaggcag ccgggagggc gcggagcccc cagggctggg cagcctgcac gccccggggc
      241 ggaccccggc tggcggggct cggacggccg cgggcttctg caccgggacg tgtcccgccg
      301 agccatgggc aacgaggcga gcttggaagg ggaagggctc cccgaagggt tggcggcggc
      361 cgcaggcggg gctggcggct ccgggagcgc cctgcacccg gggatcccag ccggcatgga
      421 ggcggacctg agccagctga gcgaggagga gaggagacag atcgctgctg tcatgtcaag
      481 ggcgcagggg ctgcccaagg gaagcgtccc cgcggccgct gcggagtcgc cctccatgca
      541 caggaaacaa gagttggata gtagtcaggc tccacagcaa cccggaaagc caccggaccc
      601 tgggcgtccc cctcagcatg gtcttagcaa aagcagaact acggacacgt ttaggtcaga
      661 gcagaagctg cctggaagga gcccgtccac tattagcttg aaagaatcca agtccagaac
      721 tgattttaag gaagaataca agtctagtat gatgcctggt ttcttctcag atgttaatcc
      781 tttaagtgct gtctcctctg ttgtaaataa attcaaccct tttgatttga tatcagactc
      841 cgaggcagtc caggaggaaa ctacaaagaa acaaaaagtt gctcagaagg accaagggaa
      901 atcggaagga atcacaaaac cttccttaca gcaaccatca cccaagttga tccctaaaca
      961 gcaaggacca gggaaggaag tgatcccgca ggatatcccc tccaagtcag tatcctccca
     1021 gcaagcagaa aaaacaaaac cacaagcccc tggcacagca aaaccttccc agcagagtcc
     1081 agcccaaaca ccagctcagc aagcaaaacc tgtagcccaa cagcctggac ctgcaaaggc
     1141 cacagtccag caaccagggc cagcaaagtc cccagctcaa ccagcaggga cagggaagtc
     1201 cccagcacaa cctccagtga cagccaagcc cccagctcaa caggctggct tagagaagac
     1261 ctcattgcag cagcctgggc caaagtctct agctcagact cctggtcagg gaaaggttcc
     1321 accagggcca gcaaagtccc cagcccagca gccagggaca gctaaactcc cagcccaaca
     1381 gcctggccca cagactgcag ctaaggtacc tgggcctaca aaaactccag ctcagctatc
     1441 tggacctgga aaaacaccag ctcagcagcc tggtccaaca aagccttcac ctcagcagcc
     1501 cattccagca aagccccagc ctcaacaacc tgttgccaca aaacctcagc ctcaacagcc
     1561 agccccagca aagccccagc ctcagcaccc aactccagca aagccccagc ctcaacaccc
     1621 aactccagca aagccccagc ctcagcagcc caccccagca aagccacagc ctcaacagcc
     1681 aactccagca aagccccagc ctcaacagcc caccccagca aagccccagc ctcaacaccc
     1741 aactccagca aagccccagc ctcaacagcc aggcctggga aaaccttcag ctcaacaacc
     1801 ttctaaatca ataagccaaa cggtaacagg aagacctctg caagcaccac caacttctgc
     1861 agcacaggcg ccagcacaag gcctctctaa aaccatctgt cctctttgca acaccactga
     1921 gcttctgttg catactccag aaaaggccaa ttttaacaca tgtactgagt gtcaaagcac
     1981 cgtgtgtagc ctctgtggtt ttaaccccaa tcctcactta actgagatca aagagtggct
     2041 ctgtttaaac tgtcagatgc aaagagctct gggaggtgaa ctggcagcaa tcccatcctc
     2101 accgcagcca accccaaagg ctgcctcagt acagccagca acagccagca aatcacctgt
     2161 accgtcacag caggcttccc caaagaagga acttccatcc aaacaggaca gccccaaagc
     2221 accggagtcc aaaaagccac cgccactggt gaaacagcca acccttcatg gacctacccc
     2281 ggctacagcc ccacagcctc cagtggcaga ggccttacct aagccagccc ctcccaagaa
     2341 gccttctgca gccctacctg aacaagcaaa ggcccctgtt gcagatgttg aaccaaagca
     2401 accgaagacg acagagacac tcacagatag cccatcttca gcagcagcaa cctcaaagcc
     2461 tgctattctg agctctcaag tgcaggcaca ggctcaagtg acaacggccc ctcctttgaa
     2521 aacagactct gccaaaactt cacagagttt cccaccaaca ggagatacaa ttaccccact
     2581 tgattctaaa gccatgccca ggcctgcatc agactcaaaa attgtttcac atcctgggcc
     2641 cacttctgag agcaaggatc cagttcagaa gaaggaggag cctaagaaag cacaaaccaa
     2701 ggtgacccct aaaccagata caaagccagt accaaaagga tccccaacac cgtctggcac
     2761 acgacctacg actggccaag ccacacccca gtctcagcag cccccgaagc ctccggagca
     2821 gtcaagacgg ttcagtttga atctgggggg tatcgctgat gcccccaaat cacagcccac
     2881 aactcctcaa gaaactgtga cagggaagct attcgggttc ggagcatcca tctttagcca
     2941 agcatcaaac ttaatttcca cagcaggtca gcaagcacct catccacaga ctgggccagc
     3001 tgccccatca aagcaagccc ctcccccatc ccagactctg gctgctcagg gaccacccaa
     3061 gtccacaggt ccgcacccat cagcacctgc caagacgact gctgtgaaga aggaaacaaa
     3121 aggcccggca gctgaaaact tagaggccaa acctgtgcaa gctccaacag tgaagaaggc
     3181 agagaaagac aagaagcccc cacctgggaa ggtcagcaaa cctccaccta cagagcccga
     3241 gaaggccgtc ctcgcccaga agccagacaa gaccaccaaa cccaaaccgg cctgtcctct
     3301 ctgcagaacg gaactcaacg tagggtctca ggatcctccc aacttcaaca cctgcacaga
     3361 atgcaagaat caagtttgca atctctgtgg atttaaccca acgccccacc tgactgagat
     3421 tcaagaatgg ctttgtttaa actgtcagac acagagagca atatcaggac aacttggaga
     3481 tatggacaaa atgccacctg catcctcagg acccaaagca tcccccgtgc ctgcccctgc
     3541 agaaccccca cctcagaaaa caccaacagc tgcccacgcg aaaggtaaaa agaaggaaac
     3601 agaagtaaaa gcagaaactg aaaaacagat cccagagaaa gaaacaccat caattgagaa
     3661 aaccccgcca gcggtcgcca cagatcaaaa actagaagaa agtgaagtaa ccaaaagcct
     3721 agtttctgtc ctgccagaaa agaaaccatc cgaggaagaa aaagccctgc ctgcagacaa
     3781 aaaggaaaag aagccacctg ctgcagaagc gccaccactt gaagaaaaga agccgatccc
     3841 tgatgatcag aaattacccc cagacgcaaa accatcggcc tcagaggggg aggagaaacg
     3901 tgatctactt aaagctcacg tacaaattcc cgaagaagga cccataggca aagtggcttc
     3961 actggcttgt gaaggagagc agcagccaga caccaggcct gaagatctgc caggcgccac
     4021 acctcagacg ttacccaagg acagacagaa ggaaagcaga gacgtaacac agcctcaggc
     4081 agaaggcact gcaaaggaag gccgagggga gcccagcaaa gacaggacag aaaaggaaga
     4141 ggacaaatca gacacttcca gttctcagca acccaagagc ccacaaggcc tgagtgacac
     4201 agggtactcc tctgacggaa tatccggctc tcttggggaa atccccagtc ttatacccag
     4261 tgatgagaaa gatttgctaa aaggactcaa aaaagactcg ttctcgcaag aaagcagccc
     4321 ttccagcccc tcagacttag cgaagttgga aagtacagta ctgtccattt tggaagctca
     4381 ggcaagcaca cttgtaggtg aaaaggcaga aaagaaaaca cagccccaga aggtttctcc
     4441 tgaacaacct caggaccagc agaaaactca gactccatcc gaaacacggg atatttctat
     4501 ttcagaagaa gagatcaaag agagtcaaga aaagaaagtt acttcaaaaa aggatagcgc
     4561 acaaggtttt ccttccagga aggagcacaa agagaatcct gagctggtcg atgacctaag
     4621 tccgcgaaga gcatcctatg attcggttga agacagcagc gaaagtgaaa actcccctgt
     4681 tgctcgcagg aagcggagaa ctagcattgg ctcatcaagc agcgaggagt ataagcagga
     4741 agacagtcaa ggctcggggg aagacgagga cttcattcga aagcagatta tagagatgag
     4801 cgctgatgag gatgcttcag gctctgagga tgaagagttc atcaggagtc agctcaaaga
     4861 gattggtggt gttactgaga gccagaagag ggaggaaaca aaggggaaag gaaaaagccc
     4921 agcagggaaa cacagacgtc tgactcgaaa gagtagtacc agcttcgacg atgatgctgg
     4981 gagacgccat tcctggcatg atgaagatga cgaaactttt gatgaaagtc ctgaactcaa
     5041 attccgagaa actaaaagcc aggagagtga ggaactagtt gttgctggag gaggagggtt
     5101 acgtcgattt aagactatag aactcaacag tacagtaaca gataagtact ctgcagaatc
     5161 ctctcagaaa aagacaactt tgtattttga tgaagagcca gaattagaaa tggaaagtct
     5221 gacggactca cccgaggaca ggtctagagg agagggatcc tcgagtctcc atgcatccag
     5281 cttcactcct ggcacgtccc ctacatcagt atcatcactt gatgaagaca gtgacagcag
     5341 cccaagtcac aaaaaaggag aaagcaagca gcagcgcaaa gctcggcaca ggtcccatgg
     5401 ccctctttta cctaccattg aagattcttc cgaggaagaa gaactacgag aggaagagga
     5461 gttattaaaa gagcaagaga agcagagaga actggagcag cagcagagga agagttctag
     5521 taagaaatcg aagaaagaca aagatgaact tcgagctcag aggaggagag aaagaccaaa
     5581 gacgccaccc agtaatctct ctcccatcga ggatgcgtct cccacagaag aattgcgcca
     5641 ggcagcagaa atggaggagc tccataggtc ctcttgttct gaatactcgc ctagcataga
     5701 atcagaccca gaaggatttg agataagccc cgagaaaata atagaagttc aaaaagttta
     5761 taaattgccc acagctgtgt ctttatactc accaacagat gagcaatctg ttatgcagaa
     5821 agaaggtgct cagaaggctt taaaaagcgc tgaggagatg tatgaagaaa tgatgcacaa
     5881 accccacaaa tataaagctt ttccagctgc aaatgaacga gacgaagtgt ttgaaaaaga
     5941 gccattgtat ggtggtatgt taatagagga ctacatttat gaatctttag tagaggacac
     6001 atacaatgga tcagtagatg gcagcctgct caccaggcaa gatgaacaaa atggatttat
     6061 gcagcagaga ggaagagagc aaaaaataag actacaggag cagatttatg atgatcctat
     6121 gcagaaaatc acagacctcc agaaagagtt ttatgaatta gaaagcttac attctattgt
     6181 gcctcaagaa gacattgttt caagctctta tatcattcca gaaagccatg agatagtgga
     6241 tctgggtagc atggtaactt ctaccagcga agaaaagaaa ctgttagatg cagatgctgc
     6301 ttatgaagaa ctcatgaagc ggcaacagat gcaagtaaca gatggatcca gcctcataca
     6361 gactaccatg ggggatgaca tggctgagtc cactctggac tttgacaggg tacaagacgc
     6421 atctttgaca tcaagtatcc tttctggagc atctcttaca gactcaacca gcagtgcaac
     6481 tctctctatc ccagatgtta agataaccca acatttttca acagaagaat ttgaggatga
     6541 atatgtaact gattatacaa gagaaattca agagataatt gcccatgaat ctctgatatt
     6601 gacctactct gagccttcag agagtgctac gtctgtccca ccttctgaca caccttctct
     6661 cacatcatct atttcttcag tttgtaccac agatagctcc tcacctgtca ctaccctcga
     6721 tagcctaaca actgtttata cggagccagc agatgtaata actaaattta aagactctga
     6781 ggaaatttct tcaacttatt ttccaggcag cgttatcgac tatccagaag atataggtgt
     6841 atccttagat cgaaccatca caccagaaag tagaactaac gctgatcaga ttatgatttc
     6901 ttttcctggt atagcaccat ctatcacaga atctgtagca actaaacctg agaggccaca
     6961 agctgacacg atttccactg acttacctat atctgaaaag gagttgataa aaggcaagaa
     7021 agaaactggg gatggaatta ttctggaagt tttggatgct tacaaagata aaagggaaga
     7081 gtctgaggct gaactaacaa aaattagctt acctgagact gggttggccc caacaccttc
     7141 ttctcaaact aaggagcaac ctggatcccc acactctgta tctggagaga tcttgggtca
     7201 ggaaaagccc acatacaggt cgccgtctgg cggtcttcct gtttctaccc atccatccaa
     7261 atctcaccca tttttccgaa gttcttcttt ggacatatca gcccagccac cccctcctcc
     7321 tccacctcct cctcctcccc cgccaccccc tcctcctccc ccacccccac cgttgcctcc
     7381 agcaacttct cctaagccac ccacttatcc taaaagaaag ttggcagctg cagctccagt
     7441 ggctccaact gctattgtta cggcccatgc tgatgctatt cctactgtag aggccacagc
     7501 tgcccgcagg agcaatgggt tacctgcaac taaaatatgt gctgctgccc ctcctcctgt
     7561 ccctcctaag ccttcttcaa ttccaactgg acttgtattt acacatagac cagaggcaag
     7621 caaacctcca attgccccca agccagcagt ccctgagatt ccagtgacta cacagaaaac
     7681 aacagacacg tgccccaaac caacaggtct acctttaaca tcaaatatgt ccttaaattt
     7741 agtgacttca gcagactaca aactgccgtc ccctacctct ccactttccc cacactcgaa
     7801 taaatcctca ccgagatatt ctaagtctct catggagact tatgtggtca ttacattgcc
     7861 ctctgaacct gggactccaa cagattcttc tgctgcccaa gccattacaa gttggccctt
     7921 gggatcaccc ccaaaagatc tggtttccct tgaaacggta ttttctgtag ttcctcccat
     7981 gacatctaca gaaattccaa gtgcttcaca gccaacccta tatacatctg gagctttggg
     8041 gacattttct gttacccctg ctgtaacagc ctctctgttt caaacagtac caacttcact
     8101 tacacagttt cttccagcag aagcttcaaa acccgaagtc tctgcagtga gtagtgcagt
     8161 cccaagtgtt gctcccagga gtgtttccat accaattcct ccagagcctc ttgctctaga
     8221 caggcatcag tataaggaaa atggaaagtt gccacttatt ggagatgcca ttgatttgcg
     8281 tacaatacca aaatcagaag ttaaagtaac tgaaaaatgt atggatcttt ctgcttctgc
     8341 aatggatgtg aaaaggcaga ccacagcgaa tgaggtttac aggcgacaaa ttagtgctgt
     8401 ccaaccttct atcataaacc tcagtgcagc ttcctcctta ggaactccag tcaccatgga
     8461 ctctaagaca gtggctgttg tcacgtgcac agacactaca atttacacaa caggcacaga
     8521 aagccaagtg ggtatagaac atgcagtaac atcaccactg cagcttacta catcaaaaca
     8581 tactgagtta caatacagaa aacctagtag ccaggctttt cccatgattc gagatgaagc
     8641 accaataaac ttatcattag gtccttccac tcaggcagta acattggctg tcacaaagcc
     8701 tgtcactgta cctcctgttg gtgttacaaa tggatggact gacagcacca tatctcaggg
     8761 gatcactgat ggagaagtgg tagatctcag cacatcaaag tctcatagga ctgttgtgac
     8821 aatggatgaa tctacttcaa atgtagtgac taaaatcata gaagatgaag aaaagccagt
     8881 tgatttgact gcaggaagac gagctgtgtg ctgtgacatg gtttacaagt tgccttttgg
     8941 acggagctgc acagcacagc agcctgcaac tacccttcct gaagaccgtt ttggttatag
     9001 ggatgaccac tatcaatatg atagatcagg gccatatggt tacagaggga ttggtggaat
     9061 gaaaccttcc atgtcagaca ctaatttagc agaagctgga cattttttct ataaaagcaa
     9121 gaatgctttt gattattctg gaggaactga agcagcagta gatctaactt cagggagagt
     9181 gtctacaggt gaggtaatgg attattcaag caagactaca ggcccatacc cagaaacacg
     9241 ccaagtcatt tcgggagttg ggattagtac cccgcagtat tccacagcaa gaatgactcc
     9301 acctcctgga ccgcagtatg gtgtgggaag tgttttgagg tcatctaatg gtgttgtcta
     9361 ttcttcagta gcaactccaa tcccctccac atttgctatc accactcaac ctggctccat
     9421 tttcagcacc acggtcaggg atttatctgg cattcataca acagatgcaa taacttcatt
     9481 atcagccctg catcaaagcc agccaatgcc tagatcgtat ttcataacaa caggtgcatc
     9541 agaaacagac atctcagtaa ccagtattga catcaatgcc agcctgcaaa ctattactat
     9601 ggaaactctt cctgctgaga caatggactc tgttcctacc ttaaccaccg cctctgaggt
     9661 gttttctgag gtggttggag aggaaagcac tcttttgatt gtccctgatg aagacaaaca
     9721 acaacagcaa cttgacttgg agcgagagct cctggaactg gagaaaatta agcaacaacg
     9781 ctttgctgag gaactggagt gggaacgtca ggagattcaa aggttccgag aacaagaaaa
     9841 gatcatggtt caaaagaagc tagaagagct gcagtctatg aagcagcacc ttctctatca
     9901 acaagaggaa gagcggcagg cccagttcat gatgagacag gagacgctag ctcaacaaca
     9961 gttacaactt gagcagatcc aacagctgca acaacagctc catcagcagc ttgaggaaca
    10021 aaaacttcgc cagatctacc agtataacta cgaaccctct gggactgctt ctccacaaac
    10081 caccactgaa caggcaattt tggagggtca gtatgttgct acagagggca gtcagttttg
    10141 ggccactgaa gatgccacca caacagcgtc tactgtggta gccattgaaa taccacagag
    10201 ccaaggatgg tacacggttc agtctgacgg tgtgactcag tacattgccc cacctggcat
    10261 cttgagcact gtttcggaga tacctctgac agatgttgtt gtaaaagagg agaaacagcc
    10321 caaaaagaga agttctggag ctaaagttcg ggggcagtat gacgagatgg gggaaagcat
    10381 ggcagatgat ccacggaatt taaaaaagat agtggacagt ggtgtacaaa ctgacgatga
    10441 agaaactgct gatcggactt atgcaagtag gagaagaaga actaaaaaga gcgttgatac
    10501 cagtgtccaa actgatgacg aagatcagga tgagtgggat atgccttcta ggtccaggag
    10561 aaaagcgcga acagggaaat acggagatag cacggcagag ggtgacaaga ccaaaccccc
    10621 ttccaaagtc tccagtgtag cagttcaaac agtcgcagag atatctgtgc aaactgagcc
    10681 attgggaacc ataagaacac cttccatacg agcacgggtg gatgccaagg tagaaataat
    10741 taaacacatt tcagcgcctg aaaagactta caaagggggc agtttaggat gccaaacaga
    10801 aacagatcca gacacacaga gccctccata tatgggtgcc acatctccac ccaaagacaa
    10861 gaaacgccca acacctttag agattggtta ctcttcttct caccttcggg cagaccccac
    10921 agtccagctg gctccttccc cacccaaatc tccaaaagtc ctttactcac ccatctcacc
    10981 actttcccca ggccacgcct tagaaccagc ctttgtacct tatgaaaaac ccctccctga
    11041 tgacataagt ccacagaaag tactccatcc agatatggct aaagttcccc cggcaagccc
    11101 taagacagcc aagatgatgc agcgttcaat gtctgacccc aagcctctga gtccaacagc
    11161 agacgaaagt tccagggctc cttttcagta ttccgagggc ttcacggcta aaggttccca
    11221 aacaacctct ggtactcaga aaaaagtgaa gagaacactg ccaaatcccc ctcctgagga
    11281 ggcgtccacg ggaacacagt caacttacag caccatgggc actgcttcta ggagaagaat
    11341 gtgcagaacc aataccatgg ctcgagccaa aattctccag gacatagacc gagagcttga
    11401 cctcgtggaa agagagtctg ccaagcttag aaagaagcaa gcagaacttg atgaagaaga
    11461 aaaggagatt gatgccaagc tacggtatct ggaaatggga attaacagga gaaaggaagc
    11521 actattgaag gagagagaaa aaagagagcg tgcctacctg caaggggtag ccgaggaccg
    11581 tgattatatg tctgacagtg aggtcagcag taccagaccc agccgagtag aaagccagca
    11641 cggcattgag cggccaagaa cagctcctca gactgaattc agccagttca taccaccaca
    11701 aacacaaaca gaagctcagc tagttcctcc gacaagtcct tacacacaat accagtactc
    11761 atcacccgct cttcccaccc aagccccgac cccatacacg caacagtctc attttcaaca
    11821 gcagacattg taccatcagc aagtgtcacc ttatcagact caaccaacct tccaagctgt
    11881 ggccaccatg tccttcacac cccaggctca acctacccca actccacagc catcctatca
    11941 attaccatca cagatgatgg tgatacaaca aaagcctcgg caaaccacat tgtatttgga
    12001 gcctaagata acttctacct atgaagtgat tcgcaaccaa cccctgatga ttgctccagt
    12061 ttctactgat aatacgtatg ctgtttccca tctcgggagt aagtacaata gtttagactt
    12121 gagaataggt ttggaggaaa gaagcagtat ggcaagcagt ccaatatcaa gcatatctgc
    12181 agactccttc tatgcagaca ttgaccacca tacttcaagg aattatgtcc taatagatga
    12241 cattggagac attaccaaag ggacagcagc tttgagctct gcatttagtc tccatgaaaa
    12301 ggatctgtcc aaaacagatc gtctccttag aaccactgag acacggaggt ctcaagaagt
    12361 gacagacttc ctagcgcctt tgcagacttc ctccagactg catagctatg tgaaggcaga
    12421 ggaggattcg atggaggatc cttatgagtt aaagcttctg aaacatcaga ttaagcaaga
    12481 attccgtagg ggcacagaga gcttagatca ccttgctggt ctctcacatt attaccatgc
    12541 tgatactagc tatagacact tccccaagtc tgaaaagtat agcatcagta gactcaccct
    12601 ggaaaaacaa gctgccaaac aattgcctgc agccatactt taccaaaagc aatcaaagca
    12661 taagaaagca ttaattgacc ctaaaatgtc taagttttca cctattcaag aaagtagaga
    12721 ccttgaacct gactatccca cttacttgag ttctagcact tcgtccattg gtggtatttc
    12781 ttctagggca aggcttcttc aagatgatat cacatttggc ctcagaaaaa atattacaga
    12841 tcaacaaaaa ttcatgggtt catcacttgg gtcaggactg ggcacgttag gaaataccat
    12901 ccggtcggct ctacaggatg aagcagataa gccatacagc agtgggagca ggtccaggcc
    12961 ttcctctagg ccttcctctg tctatgggct tgatttatca attaagaggg attcttccag
    13021 ctcatcacta agattgaaag ctcaagaggc tgaagctctg gatgtctctt ttggccattc
    13081 ttcatcttct gccagaacta agcccaccag cttaccaatc agccagagta gaggaagaat
    13141 accaattgtg gcccagaatt cagaagaaga aagtccacta agtcctgttg gccagccaat
    13201 gggaatggcc agggctgcag ctggaccttt gccgcccata tctgcagata caagggatca
    13261 atttggatca agtcactcat tgcctgaagt tcagcaacac atgagagaag agtcacggac
    13321 aaggggttat gaccgtgata tagcattcat catggatgac ttccaacatg ctatgtctga
    13381 cagtgaagcc tatcacttgc gtcgggagga aacggattgg tttgataaac ccagggagtc
    13441 tcggttggaa aatggccatg gtctggatcg gaaactgcca gaaagactgg ttcactctag
    13501 acccctcagt caacatcaag agcaaattct gcagatgaat gggaagacaa tgcactacat
    13561 ctttcctcat gcaaggataa aaataaccag agactctaag gatcacacag tttcaggtaa
    13621 tgggctagga attagaattg ttggtggtaa agaaattcct ggacatagtg gagaaattgg
    13681 agcctatatt gctaagattc ttcctggtgg aagtgcagaa cactcaggga aacttataga
    13741 agggatgcaa gttttggaat ggaatggaat cccgttaact tctaaaacat atgaagaagt
    13801 acagagcatc attaatcagc aaagtgggga agcagaaata tgtgtaagac tggacctcaa
    13861 tatgttgtca gactcggaaa atccacagca tctggaactt catgagccac caaaagttgt
    13921 ggataaggca aagtctccag gcgtggatcc caagcagctg gcggcagagc tgcagaaagt
    13981 ctcactccag cagtccccac tggtcatgtc atcagttgtg gagaaagggg ctcatgctca
    14041 ctcaggtccc acatcggcag gatccagttc tgttcccagc cccgggcagc cagggtcacc
    14101 ttcagtaagc aaaaagaagc acggcggcag caagcctact gatgtgtcca agactgcctc
    14161 tcacccaatt acaggagaga ttcagcttca aatcaactac gatcttggaa atcttataat
    14221 acatattctt caagcaagaa acctggtccc cagagacaac aatggctact cggacccatt
    14281 tgtgaaggtg tacctacttc caggacgagg tcaagtcatg gttgtccaga atgcaagtgt
    14341 tgagtacaag agaaggacta aatatgtcca gaaaagtctt aatcccgagt ggaatcaaac
    14401 agtaatttat aaaagtattt ccatggagca gctcatgaag aagaccttgg aggtgacagt
    14461 ttgggattat gatagattct catccaacga cttccttgga gaggtattga ttgatttatc
    14521 tagcacatct catctggaca acactcctcg gtggtatccg ctgaaagaac agactgaaag
    14581 cattgagcat ggcaagtctc actccagtca aaacagccag cagtccccaa agccctctgt
    14641 gatcaaaagc agaagccacg gtatcttccc cgacccatcc aaggatatgc aggttcccac
    14701 cattgagaaa tcccacagta gtcctggtag ttcaaaatca tcatctgaag gccatctccg
    14761 atctcatgga ccatctcgca gtcaaagcaa aaccagtgtc gcccagaccc acctggaaga
    14821 tgcaggggcg gccatagccg cagctgaagc cgctgtgcag caactccgca ttcaaccaac
    14881 aaagcctacc aaccaccggc ctgcagagac ctccgtgtcc actggctcct cgggcagcag
    14941 cgttggcagt gggtatagcg tggacagtga aggaagcagc tgtgtggcag gggagcctaa
    15001 tctacttcct atcccaagga taggaaagat gggacagaat ggccaagacc ctgtgaaaca
    15061 gccaggaatg ggagcagcag acacggaggc taaaactcaa gtaatgggag agatcaagct
    15121 agcactgaaa aaggaaatga aaaccgatgg tgaacaacta atagttgaaa ttctccagtg
    15181 cagaaatatt acctacaaat ttaagtctcc ggatcattta ccagatttat atgtgaaaat
    15241 atatgtgata aacattgcca cccaaaaaaa ggttattaaa aagaaaacaa gggtatgtcg
    15301 acatgatcga gagccttcct tcaatgaaac ctttagattc agcctaagtc ctgcaggcca
    15361 ctcacttcag attttacttt tctccaatgg cgggaagttt atgaagaaga ctttgattgg
    15421 agaagcttgt atctggcttg acaaagtgga tctgagaaaa agaatagtca attggcacaa
    15481 actgttgatg agtcctactc aaacacattg aagatgtgtc agctcagggc gaggaagcgg
    15541 agctccatag cctcagatcg agactgtagt tgaacactac tgtggtgatc tatgctttca
    15601 gaagcaaatg aggtcaaaac caagaagcaa gtggatggca tgactgtggg atacaaagag
    15661 actttaaaag ttcacatgtt gaaggcaaac cgatggaaat aggccagaga cactggattg
    15721 ctcatagctg tgaagagaaa ctgtgaaaga tgatttgatt ttttttaaag ataaatgtct
    15781 ttgatcagac tgagctagaa ctgtaaaaag aggcacagag gttactatgg tgttgaaagg
    15841 tagtgtgaaa gcaattgctt atacacacct aaccttgttt gaaaaaaaaa aaaaactgat
    15901 gtaatgaatc atggttttct aaatatcaaa actccttgtc ttatattctc ataaaacttc
    15961 ctgttctgga attctgacat aagattaaat tctgagttgt ctataggacc agaaggtgac
    16021 tgtgtttgtg ggggaaatta aaccagaatg atgaacaatg gattttaggt tacaaggtgc
    16081 atgctttccc tatgtctgtg ttagaaagct gtacaacagt aggtctgaat tctacgtggc
    16141 aaagcacatc agttttgctg taatttacaa tagtgaagag tgaagataat agtaggttaa
    16201 aaatgaaatt gtatttatcc agaaataaaa taaaaattag agactggata ttaaagaaga
    16261 atgttaaccc acaaaatatg taccttggtt gaaaacatta cagcctccat aattttcatc
    16321 aagtcaaggt gttctcaatt gtacacaaca aggtacctag attaatttgt gtgccctaag
    16381 aaaagggttg cttgttctct gaggaactgc tattacatgt atcatattgc ttatttcctt
    16441 tctcttgcat aatgtactgt cagagacgta aaattattgt tctgcagaca aactatattt
    16501 gtcatataac attgattaaa ctcttgtcat ttcctgattt tccatgtttt ttctctaaac
    16561 gcaatgccta cataatgaaa attctgatat caattctctt attctgcttt cttgctagtg
    16621 atatcagaat tagaaatctc ctatgataga aaggaatgta aaaagtcaat atttgccggg
    16681 cagtggtggc gcacgccttt aatcccagca cttgggaggc aaaggcaggc aaatttctga
    16741 gttcgaggcc agcctggtct acaaagtgag ttctaggaca cccagggcta cacagagaaa
    16801 ccctgtctca aaaaaccaaa aaaaaaaaaa aaaaaaaagg tcaatgtttg aaattaaacc
    16861 atatttgact tatattaata atattgactc aaacaaccag tatatgaatc atcaagatga
    16921 agcattctct ttcccatcaa ccgccaatgc ccataaagaa agtaacacag aaattgtggg
    16981 aaacacactc agctgggatg aaaatcctgg tttctagccc tgattttact ccttactttg
    17041 cacatattaa cttgcaaatg tcatttaaat tcactttccc ctgaaataat gatgtataaa
    17101 aagatatgag ataggaaagc acattcgcaa atatagcttt atatataaat ggagagttaa
    17161 tgattatgta ggtttttaat tctcagattt tatatgtgag aaacaactgc aaagcattgg
    17221 tgagcatcag catgggaaga cagtaaattg ttccacaact catttgtgtt tcgacatcag
    17281 cttccattta atatgaagaa gattgaatag taagtaacag ttatttgcca caccaaactt
    17341 attaaagtac atggttataa ccccattccc aaaccctacc caaaataaac taaattgctt
    17401 tgtggacttt ttaagctgaa aaatgttgct ttatttaaga tgaaattcta gtaggattac
    17461 aggaaatcat ctcaaactta ttggttgggt attgaatttt ctcatgttct ggagaaaaac
    17521 attaaatttt aaaacaacgt aaaacactag gagtaaagaa tttctagtat atgataagtt
    17581 gtatatctta cactgattta aataatatat aaattcacat tggttgtcct aaattaccac
    17641 cactctcttc cctccatctt ttggtccctc ctcccagata atattatgcc atttaccact
    17701 aatggtcttc taaactgaac aatgggagat gcaaattttc agaactaggg tattgctacc
    17761 attttagttt tagagactac tcgatctgct ggtgctttcc atggatgttt ccagcagaca
    17821 aatgcttgga gaagcctata actcagcaca atggaggggg gaagaagtgt aagaggatcc
    17881 tctgatatca aaaggctctg ccatttgctt ttcacagtag gctctggttc atccctgatt
    17941 ctgttccgca gcactggtac tccaagggtt ccctgaaagg ttgtatagca tggtagctta
    18001 ctccattcta gttacacctt acacagaggg accaccagag cgagattatc ctgagctatt
    18061 aagcctgaaa gaatggaagg cttcctaata ccgcttccct tccattctaa aaagctggcg
    18121 attggggtta gatcaatcag accaatcaag gaagtcattg ttggagcttg tgatcatgcg
    18181 ccagcattcc ccttgagaaa caaagatgct gccagtgtgc caagtttgca gctgacgaaa
    18241 ctcctaggtt gtaactagaa tcgctctatg caacaccagt tcttatatga atgcacttct
    18301 gagggacgct gattgccctt ctcacaacaa aactattcct tttttaattg caaatagggc
    18361 tcttagtatt ttttactttt ttgtaaataa catgatatat gatttttcat ttatatttta
    18421 ttttatgtta ttagaaatta gcttttttat cttaatagta acagtttata atctctaatt
    18481 actggataag taatcttgaa acactcaaac cttgagaagc atttcatttc cctactagtc
    18541 cattgagtca tcagggatga aaattacttg ttggtttaaa tttagtacta agaagtcttt
    18601 caacagagaa gaaaaagcac agagtgaatt tttataaaag atgggaaatt atagaatcat
    18661 tattatagat aaagataaca ctagtagcaa gaagttggtg ttttctctga ttttacttaa
    18721 tattaagaaa agttttgatg actctgaact catttgatta ccatttgtgt tttaaaagta
    18781 cagtgctcaa cattaaggaa tagtaattgc ccttgatcct taatatggtc catattttaa
    18841 tagatcatga tagttaagta tacattctct gcatttctat gaggttgact gggggaatca
    18901 tacacactag actagttgta tcctgggaat attatagctg gtaaccagct gatattgatt
    18961 ctcactatag aatggttgtt atgatgttgg tggtagtggt agtaacaaaa gtggtggcag
    19021 tgatatggag taaaatgaat tatttgttac tatattgtgc ccagtttctt agagatgatt
    19081 taatcaatac ttcatttcat tcaagtatca taaagatgtt tatagtattt ttttacttta
    19141 ttatttaaat cataactaac aatattttta aaaacttatt ttaattgcta taatgtcaaa
    19201 tagttcaaaa ttagccaact ccagctatac gtgtttataa ctatatgtat ggcagacatg
    19261 actttccctc cttttgagtt tgaactgaaa gtgaaagctc agtaaataat cctgcgcttt
    19321 tcccttaata attatttgcc tttagtgtaa cacaagaatg aatgaatgag tgaatcatat
    19381 attctcattg actatgacat attgatgttt tctgtatgtc tcatgtgact attattgaag
    19441 attcacatta aacaaatgtc atttaaccaa gttctttatc tgataggtag actgagagca
    19501 ttttctgagt ctgttatcct gtatataact agacatttgg taacacagca aactgtagag
    19561 ctatcctcat cctttggaag tcagcatgtg actgtgatta tcattcgact gtgtccgtat
    19621 atctgtttcg tgatgtgctc aggtgctccc aatactgatt aatgtgtgtg ctaataacac
    19681 atctgtttaa aatggtgttt cttgtctaag aaaagtttaa aacttaataa aatttatttc
    19741 caataaaaca tgaagttctt ggagatgcct ctcgtttctg acgatatgtt gcatgctttc
    19801 ttggtggttt gctaatattt ttattatttt tcttgtttac caaacagttt gaaaaattta
    19861 atgaacaaat gctaattctt gggctagtag ttgtataaac tgacctgaaa taaatgagaa
    19921 ttgtgtagag acaaaatgct tgtaaatctg tcttcagttt tattctatgt aaaaacatgt
    19981 cttggttttg tgactgtaca cagatatatc ttaataactt aatgtaaact gtgccgtata
    20041 aaggttgttg taccagcctt actaataaat actgaaacta tcaaaaaaaa aaaaaaaaa
//
