LOCUS       NM_028994               3420 bp    mRNA    linear   ROD 24-OCT-2024
DEFINITION  Mus musculus phosphoenolpyruvate carboxykinase 2 (mitochondrial)
            (Pck2), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_028994
VERSION     NM_028994.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Duan,K.L., Wang,T.X., You,J.W., Wang,H.N., Wang,Z.Q., Huang,Z.X.,
            Zhang,J.Y., Sun,Y.P., Xiong,Y., Guan,K.L., Ye,D., Chen,L., Liu,R.
            and Yuan,H.X.
  TITLE     PCK2 maintains intestinal homeostasis and prevents colitis by
            protecting antibody-secreting cells from oxidative stress
  JOURNAL   Immunology 173 (2), 339-359 (2024)
   PUBMED   38934051
  REMARK    GeneRIF: PCK2 maintains intestinal homeostasis and prevents colitis
            by protecting antibody-secreting cells from oxidative stress.
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Zhao,Y., Yuan,W., Feng,Y. and Zhao,R.
  TITLE     DNA 5mC and RNA m6A Collaborate to Upregulate Phosphoenolpyruvate
            Carboxykinase 2 for Kupffer Cell Activation
  JOURNAL   Int J Mol Sci 25 (18), 9894 (2024)
   PUBMED   39337381
  REMARK    GeneRIF: DNA 5mC and RNA m[6]A Collaborate to Upregulate
            Phosphoenolpyruvate Carboxykinase 2 for Kupffer Cell Activation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3420)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 3420)
  AUTHORS   Gehlen-Breitbach,S., Schmid,T., Frob,F., Rodrian,G., Weider,M.,
            Wegner,M. and Golz,L.
  TITLE     The Tip60/Ep400 chromatin remodeling complex impacts basic cellular
            functions in cranial neural crest-derived tissue during early
            orofacial development
  JOURNAL   Int J Oral Sci 15 (1), 16 (2023)
   PUBMED   37024457
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3420)
  AUTHORS   Foster,H.R., Ho,T., Potapenko,E., Sdao,S.M., Huang,S.M.,
            Lewandowski,S.L., VanDeusen,H.R., Davidson,S.M., Cardone,R.L.,
            Prentki,M., Kibbey,R.G. and Merrins,M.J.
  TITLE     beta-cell deletion of the PKm1 and PKm2 isoforms of pyruvate kinase
            in mice reveals their essential role as nutrient sensors for the
            KATP channel
  JOURNAL   Elife 11, e79422 (2022)
   PUBMED   35997256
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3420)
  AUTHORS   Stark,R., Pasquel,F., Turcu,A., Pongratz,R.L., Roden,M.,
            Cline,G.W., Shulman,G.I. and Kibbey,R.G.
  TITLE     Phosphoenolpyruvate cycling via mitochondrial phosphoenolpyruvate
            carboxykinase links anaplerosis and mitochondrial GTP with insulin
            secretion
  JOURNAL   J Biol Chem 284 (39), 26578-26590 (2009)
   PUBMED   19635791
  REMARK    GeneRIF: Data show that PEPCK-M message and protein were detected
            in islets.
REFERENCE   7  (bases 1 to 3420)
  AUTHORS   Briancon,N. and Weiss,M.C.
  TITLE     In vivo role of the HNF4alpha AF-1 activation domain revealed by
            exon swapping
  JOURNAL   EMBO J 25 (6), 1253-1262 (2006)
   PUBMED   16498401
REFERENCE   8  (bases 1 to 3420)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   9  (bases 1 to 3420)
  AUTHORS   Detter,J.C., Zhang,Q., Mules,E.H., Novak,E.K., Mishra,V.S., Li,W.,
            McMurtrie,E.B., Tchernev,V.T., Wallace,M.R., Seabra,M.C.,
            Swank,R.T. and Kingsmore,S.F.
  TITLE     Rab geranylgeranyl transferase alpha mutation in the gunmetal mouse
            reduces Rab prenylation and platelet synthesis
  JOURNAL   Proc Natl Acad Sci U S A 97 (8), 4144-4149 (2000)
   PUBMED   10737774
REFERENCE   10 (bases 1 to 3420)
  AUTHORS   Zuberi,A.R., Nguyen,H.Q., Auman,H.J., Taylor,B.A. and
            Roopenian,D.C.
  TITLE     A genetic linkage map of mouse chromosome 2 extending from
            thrombospondin to paired box gene 1, including the H3 minor
            histocompatibility complex
  JOURNAL   Genomics 33 (1), 75-84 (1996)
   PUBMED   8617512
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK030501.1 and BU757365.1.
            
            On Jan 13, 2018 this sequence version replaced NM_028994.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849374,
                              SAMN00849375 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG   :: upstream AUG and CDS
                                                  extension is not conserved
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3400              AK030501.1         2-3401
            3401-3420           BU757365.1         1-20                c
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 28.19 cM"
     gene            1..3420
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /note="phosphoenolpyruvate carboxykinase 2
                     (mitochondrial)"
                     /db_xref="GeneID:74551"
                     /db_xref="MGI:MGI:1860456"
     exon            1..248
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..2142
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /EC_number="4.1.1.32"
                     /note="phosphoenolpyruvate carboxylase"
                     /codon_start=1
                     /product="phosphoenolpyruvate carboxykinase [GTP],
                     mitochondrial"
                     /protein_id="NP_083270.2"
                     /db_xref="CCDS:CCDS27114.2"
                     /db_xref="GeneID:74551"
                     /db_xref="MGI:MGI:1860456"
                     /translation="MAAMYLPGLRLSRHGLRPWCWSPCRSIQTLHVLSGDMSQLPAGV
                     RDFVARSAHLCQPEGIHICDGTEAENTAILALLEEQGLIRKLPKYKNCWLARTDPKDV
                     ARVESKTVIVTPSQRDTVPLLAGGARGQLGNWMSPDEFQRAVDERFPGCMQGRIMYVL
                     PFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFIKCLHSVGQPL
                     TGHGDPVGQWPCNPEKTLIGHVPDQREIVSFGSGYGGNSLLGKKCFALRIASRLARDE
                     GWLAEHMLILGITNPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMR
                     FDSEGQLRAINPENGFFGVAPGTSAATNPNAMATIQSNTLFTNVAETSDGGVYWEGID
                     QPLPPGVTITSWLGKPWKPGDKEPCAHPNSRFCVPARQCPIMDPAWEAPEGVPIDAII
                     FGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKTIMHDPFAMRPFFGYNF
                     GRYLEHWLSMEGQKGARLPRIFHVNWFRRDEAGRFLWPGFGENARVLDWICRRLEGED
                     SAQETPIGLVPKEGALDLSGLSAVDTSQLFSIPKDFWEQEVRDIRGYLTEQVNQDLPK
                     EVLAELEALEGRVQKM"
     exon            249..494
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            495..679
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            680..883
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1071
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1234
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1453
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1591
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1592..1687
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..3403
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2227..2232
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /note="hexamer: ATTAAA"
     polyA_site      2255
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /note="major polyA site"
     polyA_site      2304
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
     polyA_site      2318
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
     regulatory      2911..2916
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /note="hexamer: AATACA"
     polyA_site      2934
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
     regulatory      3183..3188
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /note="hexamer: AATAAA"
     polyA_site      3208
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
     regulatory      3383..3388
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
                     /note="hexamer: ATTAAA"
     polyA_site      3403
                     /gene="Pck2"
                     /gene_synonym="1810010O14Rik; 9130022B02Rik; PEPCK-M"
ORIGIN      
        1 acccagcccg gggccttgag cccgggctag tgagccggcc tacagaaggg cgagagaacc
       61 agctaggttc cgccccgggg actgcccgcc tcctttttaa gcgcctcccg cccaacctca
      121 actccctctg gctttgctat gctccttcct tccccgcctt ccttgtctcc ccgactgcgc
      181 tcgttcctgt ggccatcccg cagcgccagt ccaggtgcca tggctgctat gtacctcccc
      241 ggcctgcggc ttagccggca cgggctaagg ccctggtgct ggtcaccgtg ccgtagcatc
      301 caaaccctgc atgtgctcag tggagatatg agtcagctgc cggctggagt tcgagacttt
      361 gtggcgcgca gtgcgcatct gtgccaacca gagggcatcc acatctgtga tgggaccgag
      421 gctgagaaca ctgccatact ggccctgctg gaagaacagg gtcttatccg caaactcccc
      481 aagtataaga actgctggct ggcccgcaca gaccccaagg atgtggcacg ggtagaaagc
      541 aagacggtga ttgtaactcc ttcgcagcgg gacacagtgc ctctcctggc tggtggggcc
      601 agggggcagc tgggcaactg gatgtcccca gatgagttcc agagagctgt ggacgagaga
      661 ttcccaggat gcatgcaggg ccgcattatg tatgtgcttc cattcagcat gggtcccgtg
      721 ggctccccac tctcccgcat tggagtgcag ctcactgact cggcttacgt ggtggccagt
      781 atgcgtatta tgacccgcct ggggacacct gtacttcagg ccctgggaga tggtgacttc
      841 atcaagtgtc tgcattcagt gggccagccc ctgactggac atggggatcc tgtgggccag
      901 tggccgtgca atccagaaaa aaccctgatt ggccacgtgc cagaccagcg ggagatcgtc
      961 tccttcggca gcggctatgg tggtaactcc ttgctgggca agaagtgctt tgccctgcgc
     1021 atcgcctctc gcctggccag ggatgagggc tggctggcag agcacatgct gattttgggc
     1081 atcaccaacc ctgcagggaa aaagcgctac gtggcagctg ctttccccag tgcctgtggc
     1141 aagaccaatc tggccatgat gaggcctgca ttgccgggct ggaaagtgga gtgtgtgggg
     1201 gatgacatcg cctggatgag gtttgacagt gaaggtcaac tccgggccat caaccctgag
     1261 aatggcttct ttggggtggc ccctggtacc tctgctgcca ccaatcccaa tgccatggcc
     1321 acaattcaga gtaatactct cttcaccaac gtggctgaga ccagtgatgg cggtgtgtac
     1381 tgggaaggca ttgaccagcc tcttccgcct ggtgtcacca taacctcgtg gctgggaaag
     1441 ccctggaaac ctggtgacaa ggaaccctgt gcacatccaa actcgcgctt ttgtgtcccg
     1501 gctcgccagt gccccatcat ggacccagcc tgggaagcac cagaaggtgt ccctattgat
     1561 gccatcatct ttggaggccg ccgacccaaa ggggtaccac tggtgtacga ggccttcaac
     1621 tggcgtcatg gggtgtttgt aggtagcgcc atgcgctctg agtccactgc cgctgcggag
     1681 cacaaaggaa agaccattat gcatgatccc tttgccatgc ggcctttttt tggctataac
     1741 tttggacgct acctggaaca ctggttgagc atggagggac aaaaaggtgc ccggctgcct
     1801 cgtatcttcc atgtcaattg gttccggaga gatgaagcag gccgcttcct gtggccaggc
     1861 tttggagaga atgctcgtgt gctagactgg atctgccgaa gattagaagg agaagacagt
     1921 gcccaagaga ctcccattgg gctagtacca aaggaaggag ccctggacct cagtggcctc
     1981 agcgcagtgg acaccagtca gctgttctcc atccccaagg acttctggga gcaggaggtt
     2041 cgtgatattc ggggctacct gacagagcaa gtcaaccagg acctgcccaa ggaggtgttg
     2101 gctgagctcg aggccctgga aggacgtgta caaaaaatgt gacctgaggc tctaggctag
     2161 caagagcaca gctcccccat ttgggtagag aacccccagg ctcacagaaa acatgaacaa
     2221 tctgacatta aaatgtgtga gtgttggaag gccaagccac aggactcccc atgctgtctg
     2281 ataagagatg cacactttta tacacgcgtt tgtttccact tccctgtgtc tgcataggct
     2341 tccttctaat cggtctcctg ttggctcgaa ggctcgaata gtaggaactt ctggaagaag
     2401 gcattcctct tctgaagaaa tgtaacctgg tacagtgatg caaaccttta atcccagagg
     2461 cagcctaacc tctgggagct ccaggcaagc ctgccaggtc tacatagcaa atgccaggtc
     2521 agccccctta atcccctccg ccaaaaagaa aaaaagaagg ccacagcaca gcaggctgct
     2581 ctgtgtttga gttagtattt ttgtcccaca tagacatgat gcagaagtcc ccttctccag
     2641 aggaagccat tccagaacca atttccgcct cctggtttcc tctcgcttcc tggaaccagg
     2701 agccacacct gttatttttt ccttgagaca aggtctcatg tagatcaggc tggctccaaa
     2761 ctcactgtgc agctaaagat aaccttggcc tcctgatcct cctctcccta cctctgacaa
     2821 tcgtgctacc aaagccagct agacaactaa cacttctaga acactatctg tattctagac
     2881 acattgtcag agtaccattt gcatttcaaa aatacagagt tggattgaac ataataacat
     2941 gtcttaaaat cctagcactt ggaaggaccc tatcacaagg caagagaatt gtatacagtg
     3001 accctgtctt caaaaaccac aaaccaaaga attatccagt gaatgcccca catctttgtg
     3061 tatatgggca gcagggggtt atttaaaaag aaaataagat ttgcagttca gagagagatg
     3121 ggaaatagga caggggaagt ggatttgatc aaacgacatt atgtatacat aaaattccca
     3181 ataataaaaa tatgaggtat tttttaaata ggcagttgga agaatggctc agtggttaag
     3241 agtaagttct gctctagaag aggactgaag ttcagcagcc acactgggct gctcacagcc
     3301 acctgtacct gcagctccag gagatctcac accctgatac tcacagggac ctcacgtgca
     3361 cacattcaca cacatacaca taattaaagt gaattttcct ataaaaaaaa aaaaaaaaaa
//
