LOCUS       NM_021543               3991 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus protocadherin 8 (Pcdh8), transcript variant 1, mRNA.
ACCESSION   NM_021543
VERSION     NM_021543.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Inoue,Y., Sawano,T., Yamaguchi,N., Inoue,S., Takayama,A.,
            Nakazawa,S., Inagaki,S., Nakatani,J. and Tanaka,H.
  TITLE     Comparative distribution of Arcadlin/Protocadherin-8 mRNA in the
            intact and ischemic brains of adult mice
  JOURNAL   J Comp Neurol 530 (11), 2033-2055 (2022)
   PUBMED   35368102
  REMARK    GeneRIF: Comparative distribution of Arcadlin/Protocadherin-8 mRNA
            in the intact and ischemic brains of adult mice.
REFERENCE   3  (bases 1 to 3991)
  AUTHORS   Takeuchi,C., Ishikawa,M., Sawano,T., Shin,Y., Mizuta,N.,
            Hasegawa,S., Tanaka,R., Tsuboi,Y., Nakatani,J., Sugiura,H.,
            Yamagata,K. and Tanaka,H.
  TITLE     Dendritic Spine Density is Increased in Arcadlin-deleted Mouse
            Hippocampus
  JOURNAL   Neuroscience 442, 296-310 (2020)
   PUBMED   32629153
REFERENCE   4  (bases 1 to 3991)
  AUTHORS   Wu,D., Schieren,I., Qian,Y., Zhang,C., Jessell,T.M. and de
            Nooij,J.C.
  TITLE     A Role for Sensory end Organ-Derived Signals in Regulating Muscle
            Spindle Proprioceptor Phenotype
  JOURNAL   J Neurosci 39 (22), 4252-4267 (2019)
   PUBMED   30926747
REFERENCE   5  (bases 1 to 3991)
  AUTHORS   Makarenkova,H., Sugiura,H., Yamagata,K. and Owens,G.
  TITLE     Alternatively spliced variants of protocadherin 8 exhibit distinct
            patterns of expression during mouse development
  JOURNAL   Biochim Biophys Acta 1681 (2-3), 150-156 (2005)
   PUBMED   15627506
REFERENCE   6  (bases 1 to 3991)
  AUTHORS   Frank,M. and Kemler,R.
  TITLE     Protocadherins
  JOURNAL   Curr Opin Cell Biol 14 (5), 557-562 (2002)
   PUBMED   12231349
  REMARK    Review article
REFERENCE   7  (bases 1 to 3991)
  AUTHORS   Nomura-Kitabayashi,A., Takahashi,Y., Kitajima,S., Inoue,T.,
            Takeda,H. and Saga,Y.
  TITLE     Hypomorphic Mesp allele distinguishes establishment of rostrocaudal
            polarity and segment border formation in somitogenesis
  JOURNAL   Development 129 (10), 2473-2481 (2002)
   PUBMED   11973278
REFERENCE   8  (bases 1 to 3991)
  AUTHORS   Ulbrich,M., Schmidt,V.C., Ronsiek,M., Mussmann,A., Bartsch,J.W.,
            Augustin,M., Jockusch,H. and Schmitt-John,T.
  TITLE     Genetic modifiers that aggravate the neurological phenotype of the
            wobbler mouse
  JOURNAL   Neuroreport 13 (4), 535-539 (2002)
   PUBMED   11930176
REFERENCE   9  (bases 1 to 3991)
  AUTHORS   Yamamoto,A., Kemp,C., Bachiller,D., Geissert,D. and De
            Robertis,E.M.
  TITLE     Mouse paraxial protocadherin is expressed in trunk mesoderm and is
            not essential for mouse development
  JOURNAL   Genesis 27 (2), 49-57 (2000)
   PUBMED   10890978
REFERENCE   10 (bases 1 to 3991)
  AUTHORS   Strehl,S., Glatt,K., Liu,Q.M., Glatt,H. and Lalande,M.
  TITLE     Characterization of two novel protocadherins (PCDH8 and PCDH9)
            localized on human chromosome 13 and mouse chromosome 14
  JOURNAL   Genomics 53 (1), 81-89 (1998)
   PUBMED   9787079
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151973.4.
            
            On Sep 13, 2022 this sequence version replaced NM_021543.4.
            
            Summary: This gene belongs to the protocadherin gene family, a
            subfamily of the cadherin superfamily. The gene encodes a type I
            transmembrane protein composed of an extracellular domain including
            6 cadherin ectodomains, a single-pass transmembrane domain and a
            cytoplasmic tail. Unlike classical cadherins, which are generally
            encoded by 15-17 exons, this gene includes only 3 exons with the
            first large exon encoding the extracellular and transmembrane
            region. Although this gene product is capable of homophilic
            interaction, it appears to affect cell-cell adhesion indirectly by
            initiating signaling events that regulate classical
            cadherin-mediated adhesion. Based on studies on this protein and
            its orthologs, this protocadherin mainly functions in developing
            embryos and the central nervous system, but can also function as a
            tumor suppressor. Alternative splicing yielding isoforms with
            unique cytoplasmic tails has been reported. [provided by RefSeq,
            Sep 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053008.1, AF231125.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2816              AC151973.4         71339-74154
            2817-3027           AC151973.4         74330-74540
            3028-3991           AC151973.4         74904-75867
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 42.76 cM"
     gene            1..3991
                     /gene="Pcdh8"
                     /gene_synonym="1700080P15Rik; Papc"
                     /note="protocadherin 8"
                     /db_xref="GeneID:18530"
                     /db_xref="MGI:MGI:1306800"
     exon            1..2816
                     /gene="Pcdh8"
                     /gene_synonym="1700080P15Rik; Papc"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..3404
                     /gene="Pcdh8"
                     /gene_synonym="1700080P15Rik; Papc"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; paraxial protocadherin; arcadlin"
                     /codon_start=1
                     /product="protocadherin-8 isoform 1 precursor"
                     /protein_id="NP_067518.2"
                     /db_xref="CCDS:CCDS27303.1"
                     /db_xref="GeneID:18530"
                     /db_xref="MGI:MGI:1306800"
                     /translation="MSPAKRWGSPCLFPLQLFSLCWVLSVAQSKTVRYSTFEEDAPGT
                     VIGTLAEDLHMKVSGDTSFRLMKQFNSSLLRVREGDGQLTVGDAGLDRERLCGPSPQC
                     VLAFDVVSFSQEQFRLVHVEVEVRDVNDHAPRFPRAQIPVEVSESAPVGTRIPLEVPV
                     DEDVGANGLQSVRLAEPHSPFRVELQTRADGAQCADLVLLQELDRESQASYSLELVAQ
                     DGGRPPRSATAALSVRVLDANDHSPAFPQGAVAEVELAEDAPVGSLLLDLDAADPDEG
                     PNGDVVFTFGARTPPEARHLFRLDPRSGRLTLAGQVDYERQDTYELDVRAQDRGPGPR
                     TATCKVIVRIRDVNDNAPEISITPLAAPGAPATSPFAAAAAAAALGGADAASSTGSGT
                     QEAGITSLVPEGAARESLVALVSTSDRDSGANGQVRCALYGHEHFRLQPAYAGSYLVV
                     TAASLDRERIAEYNLTLVAEDRGTPPLRTVRPYTVRVGDENDNAPIFTKPVYEVSVRE
                     NNPPGAYLATVAARDPDVGRNGQVTYRLVEAEVGRSGEAVSTYVSVDPATGAIYALRS
                     FDYETLRQLDVRVQASDGGSPQLSSNALVQVRVLDQNDHSPILVHPAPANGSLEVAVP
                     GRSTKDTAVARIQARDADEGANGELAFDLLQQEPREAFSIGRHTGEIMLTGDLSQEPP
                     GRVFKALLVISDGGRPPLTTTATVSFVVTAGGGSAVPASSGSPEHSRPPGSRLAPSGP
                     SLQWDTPLIVIIVLAGSCTLLLAAIIAIATTCNRRKKEVRKGGALREERPGAAGGGAS
                     APGSPDETARGTGPRPNMFDVLTFPGSGKAPFGSPAADAPPPAVAAAEVPGSEGGSAT
                     GESACHFEGQQRLRGAHAEPYGASPGFGKEPAAPPVAVWKGHSFNTISGREAEKFSGK
                     DSGKGDSDFNDSDSDISGDALKKDLINHMQSGLWACTAECKILGHSDRCWSPSCAGPN
                     VHPPPHPPAQMSTFCKSTSLPRDPLRRDNYYQAQLPKTVGLQSVYEKVLHRDYDRTVT
                     LLSPPRPGRLPDLQEIGVPLYESPPGSRYVSPKKGINENV"
     exon            2817..3027
                     /gene="Pcdh8"
                     /gene_synonym="1700080P15Rik; Papc"
                     /inference="alignment:Splign:2.1.0"
     exon            3028..3991
                     /gene="Pcdh8"
                     /gene_synonym="1700080P15Rik; Papc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3972..3977
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Pcdh8"
                     /gene_synonym="1700080P15Rik; Papc"
                     /note="hexamer: AATAAA"
     polyA_site      3991
                     /gene="Pcdh8"
                     /gene_synonym="1700080P15Rik; Papc"
                     /note="major polyA site"
ORIGIN      
        1 agctccagcc cagggaaagg aggcggaggc gccgagtgag ggtgggtctg cgcgtcctcc
       61 gagccagccc gagccggagc tgcagctttt cccgcacggg gctcgcagag cctgggactc
      121 acgctccgat ctgcacgccg gggaagactc tttaggttag cctttcggaa tagcagttgg
      181 ggattggcga tatgagtcct gcgaagcgct ggggcagccc ttgccttttc cccttgcagc
      241 tatttagtct ctgctgggtg ctctcagtgg cccagagcaa gacagtccga tacagcacgt
      301 tcgaagagga tgcccctggc acagtcatcg gaacccttgc agaagacctg cacatgaaag
      361 tgtctggaga cacaagcttc cgcctgatga agcaattcaa cagctctctg ctccgggtac
      421 gcgagggcga cgggcagctg accgtcgggg atgcgggcct ggaccgtgag cgtctgtgtg
      481 gcccgtcccc acagtgcgtg ctggccttcg atgttgtcag cttctcgcag gaacagttcc
      541 gcctggtgca cgtggaggtg gaggtgaggg atgtaaacga ccacgcgccc cgctttcccc
      601 gagcccagat cccggtggag gtctcagaga gcgctccggt aggcacacgc atccccctgg
      661 aggtgcccgt ggacgaggac gtgggtgcca acgggctgca gagtgtacgc ctagccgagc
      721 ctcacagccc cttccgcgtg gagctgcaga cgcgcgcgga cggtgcccag tgtgcggacc
      781 tggtgctgct acaggagttg gaccgcgaga gccaggcctc ctacagcctg gagctggtgg
      841 ctcaggatgg cgggcgaccg ccgcgttccg ccacagcagc cctcagcgtg cgtgtgctag
      901 atgccaatga ccacagcccg gccttcccac agggtgccgt ggcagaggtg gaattggccg
      961 aggacgcacc tgtgggatcg ctgctgctcg acctggacgc tgccgatccc gacgaaggtc
     1021 ccaacggcga cgttgtgttc accttcggcg cccgcacccc tcccgaagcc cgccacctct
     1081 tccggctcga cccgcgctct ggccgcctca ccttggctgg gcaggtggac tacgagcgcc
     1141 aggacaccta cgagctggac gtgcgggctc aagaccgagg tccagggccc cggacagcca
     1201 cctgcaaggt tatcgtgcgc atccgagacg tcaacgacaa cgctcctgaa atttctatca
     1261 ctcctctggc cgccccgggg gctccagcca cctcgccctt cgccgccgcc gccgctgccg
     1321 ccgcactcgg aggagcggac gcggcctcgt cgactgggtc cgggacacag gaagccggta
     1381 tcacctcgct ggtgccagag ggggcggcgc gcgagagtct agtggcgctg gtcagcacct
     1441 cggacaggga ctcgggcgcc aacgggcagg tgcgctgcgc cctctacggg cacgagcact
     1501 ttaggctgca gccggcctat gctggcagct acctggtggt gaccgctgca tctctggacc
     1561 gggagcgcat cgcggagtac aacctgacgc tggtggccga agaccgtggc acaccccctc
     1621 ttcgcaccgt caggccctac accgtgcgag tgggggacga gaacgacaac gcaccgatct
     1681 tcaccaagcc agtctatgag gtgtcggtcc gcgaaaacaa ccctccaggc gcctacctgg
     1741 ccacagtggc agcccgcgac cctgacgtgg gtcgcaacgg tcaagtcacc taccgcctgg
     1801 tagaggccga agtgggccgc tctggggaag ccgtgtccac ttacgtctca gtagacccgg
     1861 ctaccggagc catctacgct ctccgtagtt ttgactatga gactctgcgc cagcttgacg
     1921 tgcgtgtcca ggccagcgac ggcggttccc ctcagctttc cagcaatgct ctggtgcaag
     1981 tgcgggtgct ggaccagaat gatcattctc cgatccttgt gcatccggcg ccggctaatg
     2041 gctccctaga agtagcggtc cctggacgtt ccaccaagga cacagctgtg gcgcgcatcc
     2101 aggcccggga tgctgacgaa ggagccaacg gggaactggc ctttgatctg ctgcagcagg
     2161 agccacgcga ggccttctcc ataggccgcc acacggggga gatcatgctt actggagacc
     2221 tctcgcagga gcctcctggc cgcgtgttca aggccctgct ggtcatatcc gacggcggcc
     2281 gtccccctct caccaccact gcaaccgtca gtttcgtggt aacagccggg ggcgggtcag
     2341 ctgtgcctgc cagctccggg agcccagagc actcccggcc acccggctct cgcctcgcgc
     2401 cgtcggggcc ttcgctgcag tgggacacgc cgctgatcgt catcatcgtg ttagccggga
     2461 gctgcacgct gctgcttgca gccatcatcg ccatcgccac cacctgcaac cgccgcaaga
     2521 aggaggtgcg caaagggggg gccctccggg aagagcggcc cggggcggcg ggcggcggag
     2581 cctcggcccc tggctccccg gatgaaaccg ccaggggaac tgggcccagg cccaacatgt
     2641 tcgacgtgct caccttccct ggcagcggca aagcgccctt tggcagcccc gcggccgacg
     2701 cgcccccgcc cgcggtcgcc gcggccgaag tgccgggctc ggagggcggc agcgccactg
     2761 gggaaagcgc ctgtcacttc gaggggcagc agcggctccg aggcgcgcac gccgagcctt
     2821 acggtgcctc tccaggcttc gggaaggagc ctgctgcgcc ccctgttgca gtctggaagg
     2881 gtcattcatt caacaccatc tcgggccgag aagctgagaa gttcagtggg aaagacagcg
     2941 gcaaaggaga cagtgatttc aatgacagtg actcggacat cagcggggac gctttgaaaa
     3001 aagacctcat caaccacatg cagagtggac tgtgggcgtg tactgctgag tgcaagatcc
     3061 taggtcattc tgatcgctgc tggagcccat cctgtgccgg acccaacgta cacccccctc
     3121 ctcacccacc agcccagatg tcaaccttct gtaagagcac gtccctgcct cgggatcctc
     3181 tgcgcaggga caattactat caggcccagc tccccaagac agtagggttg cagagcgtct
     3241 atgagaaagt actgcataga gactatgaca ggacagtcac tttactctcc cctccccgtc
     3301 caggaaggct cccagacctg caggagattg gagtacccct ctatgaatcc cctcctggta
     3361 gcagatacgt gtccccgaag aagggaatca atgaaaatgt gtaatcttat cctgcatgtc
     3421 ttcacacaca tatacaggtc accctgacac gtccctaagt tattgaccgg tttcagtgtt
     3481 gtatatataa atatgcaaga tgtgccttaa aatgaagttg ttggaagcta tttccaatca
     3541 cattggtact gtttggtatt tgcaaacaaa aaaaatgtag ttaatgtaat ttttatgaaa
     3601 tgtgtgcagt atttaatttt tcttatcatg ctattggctt taatttcatt tgtggcttgc
     3661 aattttctta gtgtttttaa cctgtacatt gtgtaatgta atgtttgtat atactgaaat
     3721 tttgttatat ttttatatat taaaagctaa gtggaagtta ctgccaaagg aattgtctgt
     3781 aagacaaaaa acaaaatgtg ttggaattgc tcattgggat tttatctttc accagaaaca
     3841 acctaatgtt tgagaagttt ttgccttgcc aagtctaaca acttgtagat cttgagtctg
     3901 tggtagattt caagttactg ttatttaatt acattcggtt ctctgtaagc tctgtcactt
     3961 ataagcacag taataaagga ttcgtcatgt a
//
